Circular RNA LPAR3 sponges microRNA‐198 to facilitate esophageal cancer migration, invasion, and metastasis
In this study, we explored expression and functions of circular RNA LPAR3 (circLPAR3) in esophageal squamous cell carcinoma (ESCC). The differential expression of circular RNAs (circRNAs) in 10 ESCC and corresponding paracarcinoma tissues was analyzed through circRNA microarray, then the candidate c...
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| Veröffentlicht in: | Cancer science 2020-08, Vol.111 (8), p.2824-2836 |
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| creator | Shi, Yijun Fang, Na Li, Yadong Guo, Zizhang Jiang, Wei He, Yaozhou Ma, Zijian Chen, Yijiang |
| description | In this study, we explored expression and functions of circular RNA LPAR3 (circLPAR3) in esophageal squamous cell carcinoma (ESCC). The differential expression of circular RNAs (circRNAs) in 10 ESCC and corresponding paracarcinoma tissues was analyzed through circRNA microarray, then the candidate circRNAs were detected and verified through quantitative RT‐PCR, and a novel circRNA was screened, which was circLPAR3. Circular RNA LPAR3 showed apparently high expression in ESCC tissues and cells, which was closely correlated with the clinical stage and lymph node metastasis of ESCC patients. Circular RNA LPAR3 was mainly located in the cytoplasm of ESCC cells, which was more stable than the baseline gene. Circular RNA LPAR3 upregulated MET gene expression through sponge adsorption of microRNA (miR)‐198, activated the RAS/MAPK and the PI3K/Akt pathways, and promoted ESCC cell migration, invasion, and metastasis in vivo and in vitro. However, it had no effect on ESCC cell proliferation. Circular RNA LPAR3 can regulate the miR‐198‐MET signal axis to promote the migration, invasion, and metastasis of esophageal cancer cells, which can thereby serve as a potential diagnostic and therapeutic target of esophageal cancer.
In esophageal squamous cell carcinoma (ESCC), high circular RNA LPAR3 expression is positively correlated with lymph node metastasis and advanced tumor TNM stage. Circular RNA LPAR3 serves as a sponge of microRNA‐198 to regulate MET expression, thus promoting ESCC cell migration, invasion, and metastasis. |
| doi_str_mv | 10.1111/cas.14511 |
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In esophageal squamous cell carcinoma (ESCC), high circular RNA LPAR3 expression is positively correlated with lymph node metastasis and advanced tumor TNM stage. Circular RNA LPAR3 serves as a sponge of microRNA‐198 to regulate MET expression, thus promoting ESCC cell migration, invasion, and metastasis.</description><identifier>ISSN: 1347-9032</identifier><identifier>EISSN: 1349-7006</identifier><identifier>DOI: 10.1111/cas.14511</identifier><identifier>PMID: 32495982</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>1-Phosphatidylinositol 3-kinase ; AKT protein ; Biotechnology ; Cancer therapies ; Cell adhesion & migration ; Cell Line, Tumor ; Cell migration ; Cell Movement - genetics ; Cell proliferation ; circLPAR3 ; Circular RNA ; Cytoplasm ; DNA microarrays ; Esophageal cancer ; Esophageal Neoplasms - genetics ; Esophageal Neoplasms - pathology ; Esophageal Neoplasms - surgery ; esophageal squamous cell carcinoma ; Esophageal Squamous Cell Carcinoma - genetics ; Esophageal Squamous Cell Carcinoma - pathology ; Esophageal Squamous Cell Carcinoma - secondary ; Esophageal Squamous Cell Carcinoma - surgery ; Esophagectomy ; Esophagus ; Esophagus - pathology ; Esophagus - surgery ; Experiments ; Female ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Lung - pathology ; Lung Neoplasms - genetics ; Lung Neoplasms - secondary ; Lymph nodes ; Lymphatic Metastasis - genetics ; Male ; MAP kinase ; Medical prognosis ; MET ; MET protein ; Metastases ; Metastasis ; MicroRNAs ; MicroRNAs - metabolism ; Middle Aged ; miRNA ; miR‐198 ; Neoplasm Invasiveness - genetics ; Oligonucleotide Array Sequence Analysis ; Original ; Patients ; Proteins ; Proto-Oncogene Proteins c-met - genetics ; Receptors, Lysophosphatidic Acid - genetics ; Ribonucleic acid ; RNA ; RNA, Circular - genetics ; RNA, Circular - metabolism ; Software ; Squamous cell carcinoma ; Xenograft Model Antitumor Assays</subject><ispartof>Cancer science, 2020-08, Vol.111 (8), p.2824-2836</ispartof><rights>2020 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association</rights><rights>2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.</rights><rights>2020. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c5211-65844869385e484d53f8f4f50425b3b331c62b4214a8276e120e0279f70b67e23</cites><orcidid>0000-0002-9628-0208 ; 0000-0002-2434-5455</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7419039/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7419039/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32495982$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, Yijun</creatorcontrib><creatorcontrib>Fang, Na</creatorcontrib><creatorcontrib>Li, Yadong</creatorcontrib><creatorcontrib>Guo, Zizhang</creatorcontrib><creatorcontrib>Jiang, Wei</creatorcontrib><creatorcontrib>He, Yaozhou</creatorcontrib><creatorcontrib>Ma, Zijian</creatorcontrib><creatorcontrib>Chen, Yijiang</creatorcontrib><title>Circular RNA LPAR3 sponges microRNA‐198 to facilitate esophageal cancer migration, invasion, and metastasis</title><title>Cancer science</title><addtitle>Cancer Sci</addtitle><description>In this study, we explored expression and functions of circular RNA LPAR3 (circLPAR3) in esophageal squamous cell carcinoma (ESCC). The differential expression of circular RNAs (circRNAs) in 10 ESCC and corresponding paracarcinoma tissues was analyzed through circRNA microarray, then the candidate circRNAs were detected and verified through quantitative RT‐PCR, and a novel circRNA was screened, which was circLPAR3. Circular RNA LPAR3 showed apparently high expression in ESCC tissues and cells, which was closely correlated with the clinical stage and lymph node metastasis of ESCC patients. Circular RNA LPAR3 was mainly located in the cytoplasm of ESCC cells, which was more stable than the baseline gene. Circular RNA LPAR3 upregulated MET gene expression through sponge adsorption of microRNA (miR)‐198, activated the RAS/MAPK and the PI3K/Akt pathways, and promoted ESCC cell migration, invasion, and metastasis in vivo and in vitro. However, it had no effect on ESCC cell proliferation. Circular RNA LPAR3 can regulate the miR‐198‐MET signal axis to promote the migration, invasion, and metastasis of esophageal cancer cells, which can thereby serve as a potential diagnostic and therapeutic target of esophageal cancer.
In esophageal squamous cell carcinoma (ESCC), high circular RNA LPAR3 expression is positively correlated with lymph node metastasis and advanced tumor TNM stage. Circular RNA LPAR3 serves as a sponge of microRNA‐198 to regulate MET expression, thus promoting ESCC cell migration, invasion, and metastasis.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>AKT protein</subject><subject>Biotechnology</subject><subject>Cancer therapies</subject><subject>Cell adhesion & migration</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement - genetics</subject><subject>Cell proliferation</subject><subject>circLPAR3</subject><subject>Circular RNA</subject><subject>Cytoplasm</subject><subject>DNA microarrays</subject><subject>Esophageal cancer</subject><subject>Esophageal Neoplasms - genetics</subject><subject>Esophageal Neoplasms - pathology</subject><subject>Esophageal Neoplasms - surgery</subject><subject>esophageal squamous cell carcinoma</subject><subject>Esophageal Squamous Cell Carcinoma - genetics</subject><subject>Esophageal Squamous Cell Carcinoma - pathology</subject><subject>Esophageal Squamous Cell Carcinoma - secondary</subject><subject>Esophageal Squamous Cell Carcinoma - surgery</subject><subject>Esophagectomy</subject><subject>Esophagus</subject><subject>Esophagus - pathology</subject><subject>Esophagus - surgery</subject><subject>Experiments</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Lung - pathology</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - secondary</subject><subject>Lymph nodes</subject><subject>Lymphatic Metastasis - genetics</subject><subject>Male</subject><subject>MAP kinase</subject><subject>Medical prognosis</subject><subject>MET</subject><subject>MET protein</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>MicroRNAs</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>miRNA</subject><subject>miR‐198</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Original</subject><subject>Patients</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins c-met - genetics</subject><subject>Receptors, Lysophosphatidic Acid - genetics</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Circular - genetics</subject><subject>RNA, Circular - metabolism</subject><subject>Software</subject><subject>Squamous cell carcinoma</subject><subject>Xenograft Model Antitumor Assays</subject><issn>1347-9032</issn><issn>1349-7006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1qGzEUhUVJaJy0i75AEGQVyCT6H2kTMKb5AZMWt10LjaxxZGZGjjR28K6PkGfMk0SxU9MuUnFBl3s_Dod7APiC0TnO78KadI4Zx_gDGGDKVFEiJPY2fVkoRMkBOExpjhAVTLGP4IASpriSZADakY922ZgIJ3dDOP4-nFCYFqGbuQRbb2PI4-ffT1hJ2AdYG-sb35veQZfC4t7MnGmgNZ11MeOzaHofujPou5VJm850U9i63qRcPn0C-7Vpkvv89h-BX1dff45uivG369vRcFxYTjAuBJeMSaGo5I5JNuW0ljWrOWKEV7SiFFtBKkYwM5KUwmGCHCKlqktUidIRegQut7qLZdW6qXVdH02jF9G3Jq51MF7_u-n8vZ6FlS4ZzvdSWeDkTSCGh6VLvZ6HZeyyZ004UUIKXvL_UowSzhHGIlOnWypfM6Xo6p0PjPRrfjrnpzf5Zfb4b-M78k9gGbjYAo--cev3lfRo-GMr-QKQA6Mj</recordid><startdate>202008</startdate><enddate>202008</enddate><creator>Shi, Yijun</creator><creator>Fang, Na</creator><creator>Li, Yadong</creator><creator>Guo, Zizhang</creator><creator>Jiang, Wei</creator><creator>He, Yaozhou</creator><creator>Ma, Zijian</creator><creator>Chen, Yijiang</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9628-0208</orcidid><orcidid>https://orcid.org/0000-0002-2434-5455</orcidid></search><sort><creationdate>202008</creationdate><title>Circular RNA LPAR3 sponges microRNA‐198 to facilitate esophageal cancer migration, invasion, and metastasis</title><author>Shi, Yijun ; Fang, Na ; Li, Yadong ; Guo, Zizhang ; Jiang, Wei ; He, Yaozhou ; Ma, Zijian ; Chen, Yijiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5211-65844869385e484d53f8f4f50425b3b331c62b4214a8276e120e0279f70b67e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>AKT protein</topic><topic>Biotechnology</topic><topic>Cancer therapies</topic><topic>Cell adhesion & migration</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cell Movement - genetics</topic><topic>Cell proliferation</topic><topic>circLPAR3</topic><topic>Circular RNA</topic><topic>Cytoplasm</topic><topic>DNA microarrays</topic><topic>Esophageal cancer</topic><topic>Esophageal Neoplasms - genetics</topic><topic>Esophageal Neoplasms - pathology</topic><topic>Esophageal Neoplasms - surgery</topic><topic>esophageal squamous cell carcinoma</topic><topic>Esophageal Squamous Cell Carcinoma - genetics</topic><topic>Esophageal Squamous Cell Carcinoma - pathology</topic><topic>Esophageal Squamous Cell Carcinoma - secondary</topic><topic>Esophageal Squamous Cell Carcinoma - surgery</topic><topic>Esophagectomy</topic><topic>Esophagus</topic><topic>Esophagus - pathology</topic><topic>Esophagus - surgery</topic><topic>Experiments</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Lung - pathology</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - secondary</topic><topic>Lymph nodes</topic><topic>Lymphatic Metastasis - genetics</topic><topic>Male</topic><topic>MAP kinase</topic><topic>Medical prognosis</topic><topic>MET</topic><topic>MET protein</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>MicroRNAs</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>miRNA</topic><topic>miR‐198</topic><topic>Neoplasm Invasiveness - genetics</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Original</topic><topic>Patients</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins c-met - genetics</topic><topic>Receptors, Lysophosphatidic Acid - genetics</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Circular - genetics</topic><topic>RNA, Circular - metabolism</topic><topic>Software</topic><topic>Squamous cell carcinoma</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shi, Yijun</creatorcontrib><creatorcontrib>Fang, Na</creatorcontrib><creatorcontrib>Li, Yadong</creatorcontrib><creatorcontrib>Guo, Zizhang</creatorcontrib><creatorcontrib>Jiang, Wei</creatorcontrib><creatorcontrib>He, Yaozhou</creatorcontrib><creatorcontrib>Ma, Zijian</creatorcontrib><creatorcontrib>Chen, Yijiang</creatorcontrib><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, Yijun</au><au>Fang, Na</au><au>Li, Yadong</au><au>Guo, Zizhang</au><au>Jiang, Wei</au><au>He, Yaozhou</au><au>Ma, Zijian</au><au>Chen, Yijiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circular RNA LPAR3 sponges microRNA‐198 to facilitate esophageal cancer migration, invasion, and metastasis</atitle><jtitle>Cancer science</jtitle><addtitle>Cancer Sci</addtitle><date>2020-08</date><risdate>2020</risdate><volume>111</volume><issue>8</issue><spage>2824</spage><epage>2836</epage><pages>2824-2836</pages><issn>1347-9032</issn><eissn>1349-7006</eissn><abstract>In this study, we explored expression and functions of circular RNA LPAR3 (circLPAR3) in esophageal squamous cell carcinoma (ESCC). The differential expression of circular RNAs (circRNAs) in 10 ESCC and corresponding paracarcinoma tissues was analyzed through circRNA microarray, then the candidate circRNAs were detected and verified through quantitative RT‐PCR, and a novel circRNA was screened, which was circLPAR3. Circular RNA LPAR3 showed apparently high expression in ESCC tissues and cells, which was closely correlated with the clinical stage and lymph node metastasis of ESCC patients. Circular RNA LPAR3 was mainly located in the cytoplasm of ESCC cells, which was more stable than the baseline gene. Circular RNA LPAR3 upregulated MET gene expression through sponge adsorption of microRNA (miR)‐198, activated the RAS/MAPK and the PI3K/Akt pathways, and promoted ESCC cell migration, invasion, and metastasis in vivo and in vitro. However, it had no effect on ESCC cell proliferation. Circular RNA LPAR3 can regulate the miR‐198‐MET signal axis to promote the migration, invasion, and metastasis of esophageal cancer cells, which can thereby serve as a potential diagnostic and therapeutic target of esophageal cancer.
In esophageal squamous cell carcinoma (ESCC), high circular RNA LPAR3 expression is positively correlated with lymph node metastasis and advanced tumor TNM stage. Circular RNA LPAR3 serves as a sponge of microRNA‐198 to regulate MET expression, thus promoting ESCC cell migration, invasion, and metastasis.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>32495982</pmid><doi>10.1111/cas.14511</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-9628-0208</orcidid><orcidid>https://orcid.org/0000-0002-2434-5455</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 1-Phosphatidylinositol 3-kinase AKT protein Biotechnology Cancer therapies Cell adhesion & migration Cell Line, Tumor Cell migration Cell Movement - genetics Cell proliferation circLPAR3 Circular RNA Cytoplasm DNA microarrays Esophageal cancer Esophageal Neoplasms - genetics Esophageal Neoplasms - pathology Esophageal Neoplasms - surgery esophageal squamous cell carcinoma Esophageal Squamous Cell Carcinoma - genetics Esophageal Squamous Cell Carcinoma - pathology Esophageal Squamous Cell Carcinoma - secondary Esophageal Squamous Cell Carcinoma - surgery Esophagectomy Esophagus Esophagus - pathology Esophagus - surgery Experiments Female Gene expression Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Lung - pathology Lung Neoplasms - genetics Lung Neoplasms - secondary Lymph nodes Lymphatic Metastasis - genetics Male MAP kinase Medical prognosis MET MET protein Metastases Metastasis MicroRNAs MicroRNAs - metabolism Middle Aged miRNA miR‐198 Neoplasm Invasiveness - genetics Oligonucleotide Array Sequence Analysis Original Patients Proteins Proto-Oncogene Proteins c-met - genetics Receptors, Lysophosphatidic Acid - genetics Ribonucleic acid RNA RNA, Circular - genetics RNA, Circular - metabolism Software Squamous cell carcinoma Xenograft Model Antitumor Assays |
| title | Circular RNA LPAR3 sponges microRNA‐198 to facilitate esophageal cancer migration, invasion, and metastasis |
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