In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFR/PI3K/AKT Pathway
Context. About 15 million people worldwide suffer strokes each year and 5 million people are left with permanent disabilities which is due to the loss of local blood supply to the brain, resulting in a neurologic deficit. Panax notoginseng (Bruk.) F. H. Chen (Araliaceae) is a traditional Chinese her...
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| creator | Sun, Yikun Zhu, Lingqun Zhao, Yi-Zhou Gao, Yonghong Zhou, Dongrui Shi, Xiaowei Zou, Yihuai Cen, Kai Yang, Tiantian Wu, Shuang Chai, Limin |
| description | Context. About 15 million people worldwide suffer strokes each year and 5 million people are left with permanent disabilities which is due to the loss of local blood supply to the brain, resulting in a neurologic deficit. Panax notoginseng (Bruk.) F. H. Chen (Araliaceae) is a traditional Chinese herbal medicine widely used in the treatment of cardio-cerebrovascular diseases. Objective. This study investigated whether Panax notoginseng saponins (PNS) extracted from Panax notoginseng (Bruk.) F. H. Chen played a neuroprotective role by affecting the EGFR/PI3K/AKT pathway in oxygen-glucose deprived (OGD) SH-SY5Y cells. Materials and Methods. Different groups of OGD SH-SY5Y cells were treated with varying doses of PNS, PNS + AG1478 (a specific inhibitor of EGFR), or AG1478 for 16 hours. CCK8, Annexin V-FITC/PI apoptosis analysis, and LDH release analysis were used to determine cell viability, apoptosis rate, and amounts of LDH. Quantitative real-time PCR (q-RT-PCR) and western blotting were used to measure mRNA and proteins levels of p-EGFR/EGFR, p-PI3K/PI3K, and p-AKT/AKT in SH-SY5Y cells subjected to OGD. Results. PNS significantly enhanced cell viability, reduced apoptosis, and weakened cytotoxicity by inhibiting the release of LDH. The mRNA expression profiles of EGFR, PI3K, and AKT showed no difference between model and other groups. Additionally, ratios of p-EGFR, p-PI3K, and p-AKT to EGFR, PI3K, and AKT proteins expression, respectively, all increased significantly. Conclusions. These findings indicate that PNS enhanced neuroprotective effects by activating the EGFR/PI3K/AKT pathway and elevating phosphorylation levels in OGD SH-SY5Y cells. |
| doi_str_mv | 10.1155/2020/1403572 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7415117</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A639994653</galeid><sourcerecordid>A639994653</sourcerecordid><originalsourceid>FETCH-LOGICAL-c499t-db4cec5473dec2e5626905a14f1bd854e5c7c5e42587d4d79f85c8022858e7943</originalsourceid><addsrcrecordid>eNqNkU1vEzEQhlcIREvhxhlZ4oIEIf5cry-VoiotUSuooCBuluOdTVxt7OD1JuTf41VCCpw4eeR5_NjjtyheEvyeECHGFFM8JhwzIemj4pRITkacVtXjYy2_nxTPuu4eY6qklE-LE0YrTAlhp8V25tE3l2JA041pe5Nc8Cg0KC0BfYQ-hnUMCWxyG0DTpsnV0L013vxEPqSwcL4Dv0BfzDr4XKP5Dk0GPJvy9qCZXl1-Ht_O2PV4cn2Xj6bl1uyeF08a03bw4rCeFV8vp3cXH0Y3n65mF5ObkeVKpVE95xas4JLVYCmIkpYKC0N4Q-Z1JTgIK60ATkUla15L1VTC5tloJSqQirOz4nzvXffzFdQWfIqm1evoVibudDBO_93xbqkXYaPz1wlCZBa8OQhi-NFDl_TKdRba1ngIfacpZ1yKklOc0df_oPehjz6PN1A5DEWr8oFamBa0803I99pBqiclU0rxUrBMvdtTNoaui9Acn0ywHnLXQ-76kHvGX_055hH-HXQG3u6BpfO12br_1EFmoDEPNMlZKMF-AdrvvZc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2431749286</pqid></control><display><type>article</type><title>In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFR/PI3K/AKT Pathway</title><source>PubMed Central Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library (Open Access Collection)</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Sun, Yikun ; Zhu, Lingqun ; Zhao, Yi-Zhou ; Gao, Yonghong ; Zhou, Dongrui ; Shi, Xiaowei ; Zou, Yihuai ; Cen, Kai ; Yang, Tiantian ; Wu, Shuang ; Chai, Limin</creator><contributor>Gonçalves, Reggiani Vilela ; Reggiani Vilela Gonçalves</contributor><creatorcontrib>Sun, Yikun ; Zhu, Lingqun ; Zhao, Yi-Zhou ; Gao, Yonghong ; Zhou, Dongrui ; Shi, Xiaowei ; Zou, Yihuai ; Cen, Kai ; Yang, Tiantian ; Wu, Shuang ; Chai, Limin ; Gonçalves, Reggiani Vilela ; Reggiani Vilela Gonçalves</creatorcontrib><description>Context. About 15 million people worldwide suffer strokes each year and 5 million people are left with permanent disabilities which is due to the loss of local blood supply to the brain, resulting in a neurologic deficit. Panax notoginseng (Bruk.) F. H. Chen (Araliaceae) is a traditional Chinese herbal medicine widely used in the treatment of cardio-cerebrovascular diseases. Objective. This study investigated whether Panax notoginseng saponins (PNS) extracted from Panax notoginseng (Bruk.) F. H. Chen played a neuroprotective role by affecting the EGFR/PI3K/AKT pathway in oxygen-glucose deprived (OGD) SH-SY5Y cells. Materials and Methods. Different groups of OGD SH-SY5Y cells were treated with varying doses of PNS, PNS + AG1478 (a specific inhibitor of EGFR), or AG1478 for 16 hours. CCK8, Annexin V-FITC/PI apoptosis analysis, and LDH release analysis were used to determine cell viability, apoptosis rate, and amounts of LDH. Quantitative real-time PCR (q-RT-PCR) and western blotting were used to measure mRNA and proteins levels of p-EGFR/EGFR, p-PI3K/PI3K, and p-AKT/AKT in SH-SY5Y cells subjected to OGD. Results. PNS significantly enhanced cell viability, reduced apoptosis, and weakened cytotoxicity by inhibiting the release of LDH. The mRNA expression profiles of EGFR, PI3K, and AKT showed no difference between model and other groups. Additionally, ratios of p-EGFR, p-PI3K, and p-AKT to EGFR, PI3K, and AKT proteins expression, respectively, all increased significantly. Conclusions. These findings indicate that PNS enhanced neuroprotective effects by activating the EGFR/PI3K/AKT pathway and elevating phosphorylation levels in OGD SH-SY5Y cells.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2020/1403572</identifier><identifier>PMID: 32802113</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>1-Phosphatidylinositol 3-kinase ; AKT protein ; Analysis ; Annexin V ; Apoptosis ; Cardiovascular disease ; Cell culture ; Cell viability ; Cerebrovascular diseases ; Cholecystokinin ; Cytotoxicity ; Dehydrogenases ; Epidermal growth factor receptors ; Ethylenediaminetetraacetic acid ; Gene expression ; Glucose ; Herbal medicine ; Kinases ; Medicine, Chinese ; Neuroprotection ; Panax notoginseng ; Phosphorylation ; Polymerase chain reaction ; Proteins ; Saponins ; Software ; Statistical analysis ; Stroke ; Vascular diseases ; Western blotting</subject><ispartof>Evidence-based complementary and alternative medicine, 2020, Vol.2020 (2020), p.1-11</ispartof><rights>Copyright © 2020 Shuang Wu et al.</rights><rights>COPYRIGHT 2020 John Wiley & Sons, Inc.</rights><rights>Copyright © 2020 Shuang Wu et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2020 Shuang Wu et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-db4cec5473dec2e5626905a14f1bd854e5c7c5e42587d4d79f85c8022858e7943</citedby><cites>FETCH-LOGICAL-c499t-db4cec5473dec2e5626905a14f1bd854e5c7c5e42587d4d79f85c8022858e7943</cites><orcidid>0000-0001-8237-1734 ; 0000-0002-9207-1169 ; 0000-0002-4003-8244 ; 0000-0002-2852-5648 ; 0000-0002-3771-3528</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7415117/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7415117/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,27923,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32802113$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gonçalves, Reggiani Vilela</contributor><contributor>Reggiani Vilela Gonçalves</contributor><creatorcontrib>Sun, Yikun</creatorcontrib><creatorcontrib>Zhu, Lingqun</creatorcontrib><creatorcontrib>Zhao, Yi-Zhou</creatorcontrib><creatorcontrib>Gao, Yonghong</creatorcontrib><creatorcontrib>Zhou, Dongrui</creatorcontrib><creatorcontrib>Shi, Xiaowei</creatorcontrib><creatorcontrib>Zou, Yihuai</creatorcontrib><creatorcontrib>Cen, Kai</creatorcontrib><creatorcontrib>Yang, Tiantian</creatorcontrib><creatorcontrib>Wu, Shuang</creatorcontrib><creatorcontrib>Chai, Limin</creatorcontrib><title>In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFR/PI3K/AKT Pathway</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Context. About 15 million people worldwide suffer strokes each year and 5 million people are left with permanent disabilities which is due to the loss of local blood supply to the brain, resulting in a neurologic deficit. Panax notoginseng (Bruk.) F. H. Chen (Araliaceae) is a traditional Chinese herbal medicine widely used in the treatment of cardio-cerebrovascular diseases. Objective. This study investigated whether Panax notoginseng saponins (PNS) extracted from Panax notoginseng (Bruk.) F. H. Chen played a neuroprotective role by affecting the EGFR/PI3K/AKT pathway in oxygen-glucose deprived (OGD) SH-SY5Y cells. Materials and Methods. Different groups of OGD SH-SY5Y cells were treated with varying doses of PNS, PNS + AG1478 (a specific inhibitor of EGFR), or AG1478 for 16 hours. CCK8, Annexin V-FITC/PI apoptosis analysis, and LDH release analysis were used to determine cell viability, apoptosis rate, and amounts of LDH. Quantitative real-time PCR (q-RT-PCR) and western blotting were used to measure mRNA and proteins levels of p-EGFR/EGFR, p-PI3K/PI3K, and p-AKT/AKT in SH-SY5Y cells subjected to OGD. Results. PNS significantly enhanced cell viability, reduced apoptosis, and weakened cytotoxicity by inhibiting the release of LDH. The mRNA expression profiles of EGFR, PI3K, and AKT showed no difference between model and other groups. Additionally, ratios of p-EGFR, p-PI3K, and p-AKT to EGFR, PI3K, and AKT proteins expression, respectively, all increased significantly. Conclusions. These findings indicate that PNS enhanced neuroprotective effects by activating the EGFR/PI3K/AKT pathway and elevating phosphorylation levels in OGD SH-SY5Y cells.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>AKT protein</subject><subject>Analysis</subject><subject>Annexin V</subject><subject>Apoptosis</subject><subject>Cardiovascular disease</subject><subject>Cell culture</subject><subject>Cell viability</subject><subject>Cerebrovascular diseases</subject><subject>Cholecystokinin</subject><subject>Cytotoxicity</subject><subject>Dehydrogenases</subject><subject>Epidermal growth factor receptors</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Gene expression</subject><subject>Glucose</subject><subject>Herbal medicine</subject><subject>Kinases</subject><subject>Medicine, Chinese</subject><subject>Neuroprotection</subject><subject>Panax notoginseng</subject><subject>Phosphorylation</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Saponins</subject><subject>Software</subject><subject>Statistical analysis</subject><subject>Stroke</subject><subject>Vascular diseases</subject><subject>Western 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Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFR/PI3K/AKT Pathway</title><author>Sun, Yikun ; Zhu, Lingqun ; Zhao, Yi-Zhou ; Gao, Yonghong ; Zhou, Dongrui ; Shi, Xiaowei ; Zou, Yihuai ; Cen, Kai ; Yang, Tiantian ; Wu, Shuang ; Chai, Limin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-db4cec5473dec2e5626905a14f1bd854e5c7c5e42587d4d79f85c8022858e7943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>AKT protein</topic><topic>Analysis</topic><topic>Annexin V</topic><topic>Apoptosis</topic><topic>Cardiovascular disease</topic><topic>Cell culture</topic><topic>Cell viability</topic><topic>Cerebrovascular diseases</topic><topic>Cholecystokinin</topic><topic>Cytotoxicity</topic><topic>Dehydrogenases</topic><topic>Epidermal growth factor receptors</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Gene expression</topic><topic>Glucose</topic><topic>Herbal medicine</topic><topic>Kinases</topic><topic>Medicine, Chinese</topic><topic>Neuroprotection</topic><topic>Panax notoginseng</topic><topic>Phosphorylation</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Saponins</topic><topic>Software</topic><topic>Statistical analysis</topic><topic>Stroke</topic><topic>Vascular diseases</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Yikun</creatorcontrib><creatorcontrib>Zhu, Lingqun</creatorcontrib><creatorcontrib>Zhao, Yi-Zhou</creatorcontrib><creatorcontrib>Gao, Yonghong</creatorcontrib><creatorcontrib>Zhou, Dongrui</creatorcontrib><creatorcontrib>Shi, Xiaowei</creatorcontrib><creatorcontrib>Zou, Yihuai</creatorcontrib><creatorcontrib>Cen, Kai</creatorcontrib><creatorcontrib>Yang, 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Lingqun</au><au>Zhao, Yi-Zhou</au><au>Gao, Yonghong</au><au>Zhou, Dongrui</au><au>Shi, Xiaowei</au><au>Zou, Yihuai</au><au>Cen, Kai</au><au>Yang, Tiantian</au><au>Wu, Shuang</au><au>Chai, Limin</au><au>Gonçalves, Reggiani Vilela</au><au>Reggiani Vilela Gonçalves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFR/PI3K/AKT Pathway</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2020</date><risdate>2020</risdate><volume>2020</volume><issue>2020</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Context. About 15 million people worldwide suffer strokes each year and 5 million people are left with permanent disabilities which is due to the loss of local blood supply to the brain, resulting in a neurologic deficit. Panax notoginseng (Bruk.) F. H. Chen (Araliaceae) is a traditional Chinese herbal medicine widely used in the treatment of cardio-cerebrovascular diseases. Objective. This study investigated whether Panax notoginseng saponins (PNS) extracted from Panax notoginseng (Bruk.) F. H. Chen played a neuroprotective role by affecting the EGFR/PI3K/AKT pathway in oxygen-glucose deprived (OGD) SH-SY5Y cells. Materials and Methods. Different groups of OGD SH-SY5Y cells were treated with varying doses of PNS, PNS + AG1478 (a specific inhibitor of EGFR), or AG1478 for 16 hours. CCK8, Annexin V-FITC/PI apoptosis analysis, and LDH release analysis were used to determine cell viability, apoptosis rate, and amounts of LDH. Quantitative real-time PCR (q-RT-PCR) and western blotting were used to measure mRNA and proteins levels of p-EGFR/EGFR, p-PI3K/PI3K, and p-AKT/AKT in SH-SY5Y cells subjected to OGD. Results. PNS significantly enhanced cell viability, reduced apoptosis, and weakened cytotoxicity by inhibiting the release of LDH. The mRNA expression profiles of EGFR, PI3K, and AKT showed no difference between model and other groups. Additionally, ratios of p-EGFR, p-PI3K, and p-AKT to EGFR, PI3K, and AKT proteins expression, respectively, all increased significantly. Conclusions. These findings indicate that PNS enhanced neuroprotective effects by activating the EGFR/PI3K/AKT pathway and elevating phosphorylation levels in OGD SH-SY5Y cells.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>32802113</pmid><doi>10.1155/2020/1403572</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-8237-1734</orcidid><orcidid>https://orcid.org/0000-0002-9207-1169</orcidid><orcidid>https://orcid.org/0000-0002-4003-8244</orcidid><orcidid>https://orcid.org/0000-0002-2852-5648</orcidid><orcidid>https://orcid.org/0000-0002-3771-3528</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Evidence-based complementary and alternative medicine, 2020, Vol.2020 (2020), p.1-11 |
| issn | 1741-427X 1741-4288 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7415117 |
| source | PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); PubMed Central; Alma/SFX Local Collection |
| subjects | 1-Phosphatidylinositol 3-kinase AKT protein Analysis Annexin V Apoptosis Cardiovascular disease Cell culture Cell viability Cerebrovascular diseases Cholecystokinin Cytotoxicity Dehydrogenases Epidermal growth factor receptors Ethylenediaminetetraacetic acid Gene expression Glucose Herbal medicine Kinases Medicine, Chinese Neuroprotection Panax notoginseng Phosphorylation Polymerase chain reaction Proteins Saponins Software Statistical analysis Stroke Vascular diseases Western blotting |
| title | In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFR/PI3K/AKT Pathway |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T18%3A07%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20Vitro%20Evaluation%20of%20the%20Neuroprotective%20Effect%20of%20Panax%20notoginseng%20Saponins%20by%20Activating%20the%20EGFR/PI3K/AKT%20Pathway&rft.jtitle=Evidence-based%20complementary%20and%20alternative%20medicine&rft.au=Sun,%20Yikun&rft.date=2020&rft.volume=2020&rft.issue=2020&rft.spage=1&rft.epage=11&rft.pages=1-11&rft.issn=1741-427X&rft.eissn=1741-4288&rft_id=info:doi/10.1155/2020/1403572&rft_dat=%3Cgale_pubme%3EA639994653%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2431749286&rft_id=info:pmid/32802113&rft_galeid=A639994653&rfr_iscdi=true |