Diagnostic benefits of adding EspC, EspF and Rv2348-B to the QuantiFERON Gold In-tube antigen combination
Interferon (IFN)-γ release assays (IGRAs) are used to diagnose latent tuberculosis (TB) infection (LTBI). To improve the accuracy of these tests, different approaches, such as alternative cytokine detection and using different antigens, are considered. Following this purpose, this study aims to eval...
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| creator | Villar-Hernández, R. Blauenfeldt, T. García-García, E. Muriel-Moreno, B. De Souza-Galvão, M. L. Millet, J. P. Sabriá, F. Sánchez-Montalvá, A. Ruiz-Manzano, J. Pilarte, J. Jiménez, M. A. Centeno, C. Martos, C. Molina-Pinargote, I. González-Díaz, Y. D. Santiago, J. Cantos, A. Casas, I. Guerola, R. M. Prat, C. Andersen, P. Latorre, I. Ruhwald, M. Domínguez, J. |
| description | Interferon (IFN)-γ release assays (IGRAs) are used to diagnose latent tuberculosis (TB) infection (LTBI). To improve the accuracy of these tests, different approaches, such as alternative cytokine detection and using different antigens, are considered. Following this purpose, this study aims to evaluate the addition of EspC, EspF and Rv2348-B to those present in the QuantiFERON-TB Gold In-Tube (QFN-G-IT). We included 115 subjects: 74 active TB patients, 17 LTBI individuals and 24 healthy controls. Whole blood samples were collected in QFN-G-IT and in-house tubes containing different combinations of EspC, EspF and Rv2348-B, together with ESAT-6, CFP-10, and TB7.7. After overnight incubation at 37 ºC, plasma was harvested and IFN-γ quantified. IFN-γ levels in the QFN-G-IT and in-house tubes correlated very good (Spearman Rho(r) > 0.86). In-house antigen combinations distinguished healthy individuals from those with active TB and LTBI (specificities and sensitivities higher than 87.5% and 96.3%, respectively [AUC > 0.938]). Adding EspC, EspF and Rv2348-B, increased the sensitivity of the test, being the addition of EspC and Rv2348-B the combination that yielded a higher sensitivity with no specificity loss. Addition of these antigens could improve diagnosis in patients with impaired or immature immune response who are at high risk of developing TB. |
| doi_str_mv | 10.1038/s41598-020-70204-w |
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L. ; Millet, J. P. ; Sabriá, F. ; Sánchez-Montalvá, A. ; Ruiz-Manzano, J. ; Pilarte, J. ; Jiménez, M. A. ; Centeno, C. ; Martos, C. ; Molina-Pinargote, I. ; González-Díaz, Y. D. ; Santiago, J. ; Cantos, A. ; Casas, I. ; Guerola, R. M. ; Prat, C. ; Andersen, P. ; Latorre, I. ; Ruhwald, M. ; Domínguez, J.</creator><creatorcontrib>Villar-Hernández, R. ; Blauenfeldt, T. ; García-García, E. ; Muriel-Moreno, B. ; De Souza-Galvão, M. L. ; Millet, J. P. ; Sabriá, F. ; Sánchez-Montalvá, A. ; Ruiz-Manzano, J. ; Pilarte, J. ; Jiménez, M. A. ; Centeno, C. ; Martos, C. ; Molina-Pinargote, I. ; González-Díaz, Y. D. ; Santiago, J. ; Cantos, A. ; Casas, I. ; Guerola, R. M. ; Prat, C. ; Andersen, P. ; Latorre, I. ; Ruhwald, M. ; Domínguez, J.</creatorcontrib><description>Interferon (IFN)-γ release assays (IGRAs) are used to diagnose latent tuberculosis (TB) infection (LTBI). To improve the accuracy of these tests, different approaches, such as alternative cytokine detection and using different antigens, are considered. Following this purpose, this study aims to evaluate the addition of EspC, EspF and Rv2348-B to those present in the QuantiFERON-TB Gold In-Tube (QFN-G-IT). We included 115 subjects: 74 active TB patients, 17 LTBI individuals and 24 healthy controls. Whole blood samples were collected in QFN-G-IT and in-house tubes containing different combinations of EspC, EspF and Rv2348-B, together with ESAT-6, CFP-10, and TB7.7. After overnight incubation at 37 ºC, plasma was harvested and IFN-γ quantified. IFN-γ levels in the QFN-G-IT and in-house tubes correlated very good (Spearman Rho(r) > 0.86). In-house antigen combinations distinguished healthy individuals from those with active TB and LTBI (specificities and sensitivities higher than 87.5% and 96.3%, respectively [AUC > 0.938]). Adding EspC, EspF and Rv2348-B, increased the sensitivity of the test, being the addition of EspC and Rv2348-B the combination that yielded a higher sensitivity with no specificity loss. Addition of these antigens could improve diagnosis in patients with impaired or immature immune response who are at high risk of developing TB.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-70204-w</identifier><identifier>PMID: 32764560</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>692/53/2421 ; 692/699/255/1856 ; Adult ; Antigens ; Antigens, Bacterial - immunology ; Case-Control Studies ; Early Diagnosis ; ESAT-6 antigen ; Female ; Gold ; Humanities and Social Sciences ; Humans ; Immune response ; Interferon-gamma Release Tests ; Latent Tuberculosis - diagnosis ; Male ; Middle Aged ; multidisciplinary ; Mycobacterium tuberculosis - immunology ; Science ; Science (multidisciplinary) ; Sensitivity analysis ; Sensitivity and Specificity ; Spain ; Tuberculin Test ; Tuberculosis ; Tuberculosis - diagnosis ; Tuberculosis - immunology ; γ-Interferon</subject><ispartof>Scientific reports, 2020-08, Vol.10 (1), p.13234, Article 13234</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-388a9e2f3eaadf9a03ef4750fa398bdd7176151c51673020433eeccd7e698cca3</citedby><cites>FETCH-LOGICAL-c474t-388a9e2f3eaadf9a03ef4750fa398bdd7176151c51673020433eeccd7e698cca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7413380/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7413380/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,41099,42168,51555,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32764560$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Villar-Hernández, R.</creatorcontrib><creatorcontrib>Blauenfeldt, T.</creatorcontrib><creatorcontrib>García-García, E.</creatorcontrib><creatorcontrib>Muriel-Moreno, B.</creatorcontrib><creatorcontrib>De Souza-Galvão, M. L.</creatorcontrib><creatorcontrib>Millet, J. P.</creatorcontrib><creatorcontrib>Sabriá, F.</creatorcontrib><creatorcontrib>Sánchez-Montalvá, A.</creatorcontrib><creatorcontrib>Ruiz-Manzano, J.</creatorcontrib><creatorcontrib>Pilarte, J.</creatorcontrib><creatorcontrib>Jiménez, M. A.</creatorcontrib><creatorcontrib>Centeno, C.</creatorcontrib><creatorcontrib>Martos, C.</creatorcontrib><creatorcontrib>Molina-Pinargote, I.</creatorcontrib><creatorcontrib>González-Díaz, Y. D.</creatorcontrib><creatorcontrib>Santiago, J.</creatorcontrib><creatorcontrib>Cantos, A.</creatorcontrib><creatorcontrib>Casas, I.</creatorcontrib><creatorcontrib>Guerola, R. 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Whole blood samples were collected in QFN-G-IT and in-house tubes containing different combinations of EspC, EspF and Rv2348-B, together with ESAT-6, CFP-10, and TB7.7. After overnight incubation at 37 ºC, plasma was harvested and IFN-γ quantified. IFN-γ levels in the QFN-G-IT and in-house tubes correlated very good (Spearman Rho(r) > 0.86). In-house antigen combinations distinguished healthy individuals from those with active TB and LTBI (specificities and sensitivities higher than 87.5% and 96.3%, respectively [AUC > 0.938]). Adding EspC, EspF and Rv2348-B, increased the sensitivity of the test, being the addition of EspC and Rv2348-B the combination that yielded a higher sensitivity with no specificity loss. 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We included 115 subjects: 74 active TB patients, 17 LTBI individuals and 24 healthy controls. Whole blood samples were collected in QFN-G-IT and in-house tubes containing different combinations of EspC, EspF and Rv2348-B, together with ESAT-6, CFP-10, and TB7.7. After overnight incubation at 37 ºC, plasma was harvested and IFN-γ quantified. IFN-γ levels in the QFN-G-IT and in-house tubes correlated very good (Spearman Rho(r) > 0.86). In-house antigen combinations distinguished healthy individuals from those with active TB and LTBI (specificities and sensitivities higher than 87.5% and 96.3%, respectively [AUC > 0.938]). Adding EspC, EspF and Rv2348-B, increased the sensitivity of the test, being the addition of EspC and Rv2348-B the combination that yielded a higher sensitivity with no specificity loss. Addition of these antigens could improve diagnosis in patients with impaired or immature immune response who are at high risk of developing TB.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32764560</pmid><doi>10.1038/s41598-020-70204-w</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 692/53/2421 692/699/255/1856 Adult Antigens Antigens, Bacterial - immunology Case-Control Studies Early Diagnosis ESAT-6 antigen Female Gold Humanities and Social Sciences Humans Immune response Interferon-gamma Release Tests Latent Tuberculosis - diagnosis Male Middle Aged multidisciplinary Mycobacterium tuberculosis - immunology Science Science (multidisciplinary) Sensitivity analysis Sensitivity and Specificity Spain Tuberculin Test Tuberculosis Tuberculosis - diagnosis Tuberculosis - immunology γ-Interferon |
| title | Diagnostic benefits of adding EspC, EspF and Rv2348-B to the QuantiFERON Gold In-tube antigen combination |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T22%3A44%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Diagnostic%20benefits%20of%20adding%20EspC,%20EspF%20and%20Rv2348-B%20to%20the%20QuantiFERON%20Gold%20In-tube%20antigen%20combination&rft.jtitle=Scientific%20reports&rft.au=Villar-Hern%C3%A1ndez,%20R.&rft.date=2020-08-06&rft.volume=10&rft.issue=1&rft.spage=13234&rft.pages=13234-&rft.artnum=13234&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-020-70204-w&rft_dat=%3Cproquest_pubme%3E2430817741%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2430817741&rft_id=info:pmid/32764560&rfr_iscdi=true |