Insights on the Larvicidal Mechanism of Action of Fractions and Compounds from Aerial Parts of Helicteres velutina K. Schum against Aedes aegypti L
Viral diseases transmitted by the femaleAedes aegyptiL. are considered a major public health problem. The aerial parts ofHelicteres velutinaK. Schum (Sterculiaceae) have demonstrated potential insecticidal and larvicidal activity against this vector. The objective of this research was to investigate...
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| Veröffentlicht in: | Molecules (Basel, Switzerland) Switzerland), 2020-07, Vol.25 (13), p.3015, Article 3015 |
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| creator | Fernandes, Diegina A. Oliveira, Louise H. G. Rique, Hyago L. de Souza, Maria de Fatima Vanderlei Nunes, Fabiola da Cruz |
| description | Viral diseases transmitted by the femaleAedes aegyptiL. are considered a major public health problem. The aerial parts ofHelicteres velutinaK. Schum (Sterculiaceae) have demonstrated potential insecticidal and larvicidal activity against this vector. The objective of this research was to investigate the mechanisms of action involved in the larvicidal activity of this species. The cytotoxicity activity ofH. velutinafractions and compounds of crude ethanolic extract of the aerial parts of this species was assessed by using fluorescence microscopy and propidium iodide staining. In addition, the production of nitric oxide (NO) and hemocyte recruitment were checked after different periods of exposure. The fluorescence microscopy revealed an increasing in larvae cell necrosis for the dichloromethane fraction, 7,4 '-di-O-methyl-8-O-sulphate flavone and hexane fraction (15.4, 11.0, and 7.0%, respectively). The tiliroside did not show necrotic cells, which showed the same result as that seen in the negative control. The NO concentration in hemolymph after 24 h exposure was significantly greater for the dichloromethane fraction and the 7,4 '-di-O-methyl-8-O-sulphate flavone (123.8 and 56.2 mu M, respectively) when compared to the hexane fraction and tiliroside (10.8 and 8.3 mu M, respectively). The presence of plasmocytes only in the dichloromethane fraction and 7,4 '-di-O-methyl-8-O-sulphate flavone treatments suggest that these would be the hemocytes responsible for the highest NO production, acting as a defense agent. Our results showed that the larvicidal activity developed byH. velutinacompounds is related to its hemocyte necrotizing activity and alteration in NO production. |
| doi_str_mv | 10.3390/molecules25133015 |
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Schum against Aedes aegypti L</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Fernandes, Diegina A. ; Oliveira, Louise H. G. ; Rique, Hyago L. ; de Souza, Maria de Fatima Vanderlei ; Nunes, Fabiola da Cruz</creator><creatorcontrib>Fernandes, Diegina A. ; Oliveira, Louise H. G. ; Rique, Hyago L. ; de Souza, Maria de Fatima Vanderlei ; Nunes, Fabiola da Cruz</creatorcontrib><description>Viral diseases transmitted by the femaleAedes aegyptiL. are considered a major public health problem. The aerial parts ofHelicteres velutinaK. Schum (Sterculiaceae) have demonstrated potential insecticidal and larvicidal activity against this vector. The objective of this research was to investigate the mechanisms of action involved in the larvicidal activity of this species. The cytotoxicity activity ofH. velutinafractions and compounds of crude ethanolic extract of the aerial parts of this species was assessed by using fluorescence microscopy and propidium iodide staining. In addition, the production of nitric oxide (NO) and hemocyte recruitment were checked after different periods of exposure. The fluorescence microscopy revealed an increasing in larvae cell necrosis for the dichloromethane fraction, 7,4 '-di-O-methyl-8-O-sulphate flavone and hexane fraction (15.4, 11.0, and 7.0%, respectively). The tiliroside did not show necrotic cells, which showed the same result as that seen in the negative control. The NO concentration in hemolymph after 24 h exposure was significantly greater for the dichloromethane fraction and the 7,4 '-di-O-methyl-8-O-sulphate flavone (123.8 and 56.2 mu M, respectively) when compared to the hexane fraction and tiliroside (10.8 and 8.3 mu M, respectively). The presence of plasmocytes only in the dichloromethane fraction and 7,4 '-di-O-methyl-8-O-sulphate flavone treatments suggest that these would be the hemocytes responsible for the highest NO production, acting as a defense agent. Our results showed that the larvicidal activity developed byH. velutinacompounds is related to its hemocyte necrotizing activity and alteration in NO production.</description><identifier>ISSN: 1420-3049</identifier><identifier>EISSN: 1420-3049</identifier><identifier>DOI: 10.3390/molecules25133015</identifier><identifier>PMID: 32630318</identifier><language>eng</language><publisher>BASEL: Mdpi</publisher><subject><![CDATA[7,4′-di-O-methyl-8-O-sulphate flavone ; Aedes - drug effects ; Aedes - growth & development ; Aedes aegypti ; Animals ; Biochemistry & Molecular Biology ; Chemistry ; Chemistry, Multidisciplinary ; Cytotoxicity ; Dichloromethane ; Disease transmission ; Exposure ; Female ; Flavonoids ; Fluorescence ; Fluorescence microscopy ; Helicteres velutina ; Hemocytes ; Hemolymph ; Immune system ; Insecticides - pharmacology ; Iodides ; Larva - drug effects ; Larva - growth & development ; Larvae ; larvicidal activity ; Larvicides ; Life Sciences & Biomedicine ; Malvaceae - chemistry ; Microscopy ; Morphology ; Mortality ; Mosquito Vectors - drug effects ; Mosquito Vectors - growth & development ; Mosquitoes ; Necrosis ; Nitric oxide ; Pest control ; Physical Sciences ; Plant Extracts - pharmacology ; Plant Leaves - chemistry ; Propidium iodide ; Public health ; Science & Technology ; Software ; Studies ; Sulfates ; tiliroside ; Viral diseases]]></subject><ispartof>Molecules (Basel, Switzerland), 2020-07, Vol.25 (13), p.3015, Article 3015</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>8</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000550309100001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c493t-1848b4bd06b1f9e344517e2c524d267584a71d524353d9293255b7faece823a93</citedby><cites>FETCH-LOGICAL-c493t-1848b4bd06b1f9e344517e2c524d267584a71d524353d9293255b7faece823a93</cites><orcidid>0000-0001-6120-761X ; 0000-0002-1014-2411 ; 0000-0002-9936-7130 ; 0000-0001-5874-8626</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412497/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412497/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2115,27929,27930,28253,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32630318$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fernandes, Diegina A.</creatorcontrib><creatorcontrib>Oliveira, Louise H. G.</creatorcontrib><creatorcontrib>Rique, Hyago L.</creatorcontrib><creatorcontrib>de Souza, Maria de Fatima Vanderlei</creatorcontrib><creatorcontrib>Nunes, Fabiola da Cruz</creatorcontrib><title>Insights on the Larvicidal Mechanism of Action of Fractions and Compounds from Aerial Parts of Helicteres velutina K. Schum against Aedes aegypti L</title><title>Molecules (Basel, Switzerland)</title><addtitle>MOLECULES</addtitle><addtitle>Molecules</addtitle><description>Viral diseases transmitted by the femaleAedes aegyptiL. are considered a major public health problem. The aerial parts ofHelicteres velutinaK. Schum (Sterculiaceae) have demonstrated potential insecticidal and larvicidal activity against this vector. The objective of this research was to investigate the mechanisms of action involved in the larvicidal activity of this species. The cytotoxicity activity ofH. velutinafractions and compounds of crude ethanolic extract of the aerial parts of this species was assessed by using fluorescence microscopy and propidium iodide staining. In addition, the production of nitric oxide (NO) and hemocyte recruitment were checked after different periods of exposure. The fluorescence microscopy revealed an increasing in larvae cell necrosis for the dichloromethane fraction, 7,4 '-di-O-methyl-8-O-sulphate flavone and hexane fraction (15.4, 11.0, and 7.0%, respectively). The tiliroside did not show necrotic cells, which showed the same result as that seen in the negative control. The NO concentration in hemolymph after 24 h exposure was significantly greater for the dichloromethane fraction and the 7,4 '-di-O-methyl-8-O-sulphate flavone (123.8 and 56.2 mu M, respectively) when compared to the hexane fraction and tiliroside (10.8 and 8.3 mu M, respectively). The presence of plasmocytes only in the dichloromethane fraction and 7,4 '-di-O-methyl-8-O-sulphate flavone treatments suggest that these would be the hemocytes responsible for the highest NO production, acting as a defense agent. Our results showed that the larvicidal activity developed byH. velutinacompounds is related to its hemocyte necrotizing activity and alteration in NO production.</description><subject>7,4′-di-O-methyl-8-O-sulphate flavone</subject><subject>Aedes - drug effects</subject><subject>Aedes - growth & development</subject><subject>Aedes aegypti</subject><subject>Animals</subject><subject>Biochemistry & Molecular Biology</subject><subject>Chemistry</subject><subject>Chemistry, Multidisciplinary</subject><subject>Cytotoxicity</subject><subject>Dichloromethane</subject><subject>Disease transmission</subject><subject>Exposure</subject><subject>Female</subject><subject>Flavonoids</subject><subject>Fluorescence</subject><subject>Fluorescence microscopy</subject><subject>Helicteres velutina</subject><subject>Hemocytes</subject><subject>Hemolymph</subject><subject>Immune system</subject><subject>Insecticides - pharmacology</subject><subject>Iodides</subject><subject>Larva - drug effects</subject><subject>Larva - growth & development</subject><subject>Larvae</subject><subject>larvicidal activity</subject><subject>Larvicides</subject><subject>Life Sciences & Biomedicine</subject><subject>Malvaceae - chemistry</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>Mortality</subject><subject>Mosquito Vectors - drug effects</subject><subject>Mosquito Vectors - growth & development</subject><subject>Mosquitoes</subject><subject>Necrosis</subject><subject>Nitric oxide</subject><subject>Pest control</subject><subject>Physical Sciences</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves - chemistry</subject><subject>Propidium iodide</subject><subject>Public health</subject><subject>Science & Technology</subject><subject>Software</subject><subject>Studies</subject><subject>Sulfates</subject><subject>tiliroside</subject><subject>Viral diseases</subject><issn>1420-3049</issn><issn>1420-3049</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNqNks1uEzEUhUcIREvhAdggSyxRin_jmQ1SNGppRBBIwNry2HcSRzN2sD1BfQ5eGKcpUSs2rHzt-51jX_lU1WuCLxlr8PsxDGCmARIVhDFMxJPqnHCKZwzz5umD-qx6kdIWY0o4Ec-rM0bnDDNSn1e_lz659SYnFDzKG0ArHffOOKsH9BnMRnuXRhR6tDDZFaRU11Hf1Qlpb1Ebxl2YvE2oj2FEC4iuSL_qeLDs0Q0MzmSIkNAehik7r9GnS_TNbKYR6bV2PuUisqWvYX27yw6tXlbPej0keHW_XlQ_rq--tzez1ZePy3axmhnesDwjNa873lk870jfAONcEAnUCMotnUtRcy2JLTsmmG1ow6gQnew1GKgp0w27qJZHXxv0Vu2iG3W8VUE7dXcQ4lqVMZwZQFFpJK814L4T3EjWEYwbAXJObCdJp4vXh6PXbupGsAZ8jnp4ZPq4491GrcNeSU4ob2QxeHtvEMPPCVJW2zBFX-ZXtHwjY7RmvFDkSJkYUorQn24gWB0yof7JRNG8efi0k-JvCArw7gj8gi70yTjwBk4YxlgIzHBTRsaYFLr-f7p1WR-y0paIZPYHkarWDA</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Fernandes, Diegina A.</creator><creator>Oliveira, Louise H. 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Schum against Aedes aegypti L</title><author>Fernandes, Diegina A. ; Oliveira, Louise H. G. ; Rique, Hyago L. ; de Souza, Maria de Fatima Vanderlei ; Nunes, Fabiola da Cruz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-1848b4bd06b1f9e344517e2c524d267584a71d524353d9293255b7faece823a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>7,4′-di-O-methyl-8-O-sulphate flavone</topic><topic>Aedes - drug effects</topic><topic>Aedes - growth & development</topic><topic>Aedes aegypti</topic><topic>Animals</topic><topic>Biochemistry & Molecular Biology</topic><topic>Chemistry</topic><topic>Chemistry, Multidisciplinary</topic><topic>Cytotoxicity</topic><topic>Dichloromethane</topic><topic>Disease transmission</topic><topic>Exposure</topic><topic>Female</topic><topic>Flavonoids</topic><topic>Fluorescence</topic><topic>Fluorescence microscopy</topic><topic>Helicteres velutina</topic><topic>Hemocytes</topic><topic>Hemolymph</topic><topic>Immune system</topic><topic>Insecticides - pharmacology</topic><topic>Iodides</topic><topic>Larva - drug effects</topic><topic>Larva - growth & development</topic><topic>Larvae</topic><topic>larvicidal activity</topic><topic>Larvicides</topic><topic>Life Sciences & Biomedicine</topic><topic>Malvaceae - chemistry</topic><topic>Microscopy</topic><topic>Morphology</topic><topic>Mortality</topic><topic>Mosquito Vectors - drug effects</topic><topic>Mosquito Vectors - growth & development</topic><topic>Mosquitoes</topic><topic>Necrosis</topic><topic>Nitric oxide</topic><topic>Pest control</topic><topic>Physical Sciences</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves - chemistry</topic><topic>Propidium iodide</topic><topic>Public health</topic><topic>Science & Technology</topic><topic>Software</topic><topic>Studies</topic><topic>Sulfates</topic><topic>tiliroside</topic><topic>Viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernandes, Diegina A.</creatorcontrib><creatorcontrib>Oliveira, Louise H. G.</creatorcontrib><creatorcontrib>Rique, Hyago L.</creatorcontrib><creatorcontrib>de Souza, Maria de Fatima Vanderlei</creatorcontrib><creatorcontrib>Nunes, Fabiola da Cruz</creatorcontrib><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Molecules (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernandes, Diegina A.</au><au>Oliveira, Louise H. G.</au><au>Rique, Hyago L.</au><au>de Souza, Maria de Fatima Vanderlei</au><au>Nunes, Fabiola da Cruz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insights on the Larvicidal Mechanism of Action of Fractions and Compounds from Aerial Parts of Helicteres velutina K. Schum against Aedes aegypti L</atitle><jtitle>Molecules (Basel, Switzerland)</jtitle><stitle>MOLECULES</stitle><addtitle>Molecules</addtitle><date>2020-07-01</date><risdate>2020</risdate><volume>25</volume><issue>13</issue><spage>3015</spage><pages>3015-</pages><artnum>3015</artnum><issn>1420-3049</issn><eissn>1420-3049</eissn><abstract>Viral diseases transmitted by the femaleAedes aegyptiL. are considered a major public health problem. The aerial parts ofHelicteres velutinaK. Schum (Sterculiaceae) have demonstrated potential insecticidal and larvicidal activity against this vector. The objective of this research was to investigate the mechanisms of action involved in the larvicidal activity of this species. The cytotoxicity activity ofH. velutinafractions and compounds of crude ethanolic extract of the aerial parts of this species was assessed by using fluorescence microscopy and propidium iodide staining. In addition, the production of nitric oxide (NO) and hemocyte recruitment were checked after different periods of exposure. The fluorescence microscopy revealed an increasing in larvae cell necrosis for the dichloromethane fraction, 7,4 '-di-O-methyl-8-O-sulphate flavone and hexane fraction (15.4, 11.0, and 7.0%, respectively). The tiliroside did not show necrotic cells, which showed the same result as that seen in the negative control. The NO concentration in hemolymph after 24 h exposure was significantly greater for the dichloromethane fraction and the 7,4 '-di-O-methyl-8-O-sulphate flavone (123.8 and 56.2 mu M, respectively) when compared to the hexane fraction and tiliroside (10.8 and 8.3 mu M, respectively). The presence of plasmocytes only in the dichloromethane fraction and 7,4 '-di-O-methyl-8-O-sulphate flavone treatments suggest that these would be the hemocytes responsible for the highest NO production, acting as a defense agent. Our results showed that the larvicidal activity developed byH. velutinacompounds is related to its hemocyte necrotizing activity and alteration in NO production.</abstract><cop>BASEL</cop><pub>Mdpi</pub><pmid>32630318</pmid><doi>10.3390/molecules25133015</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-6120-761X</orcidid><orcidid>https://orcid.org/0000-0002-1014-2411</orcidid><orcidid>https://orcid.org/0000-0002-9936-7130</orcidid><orcidid>https://orcid.org/0000-0001-5874-8626</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 7,4′-di-O-methyl-8-O-sulphate flavone Aedes - drug effects Aedes - growth & development Aedes aegypti Animals Biochemistry & Molecular Biology Chemistry Chemistry, Multidisciplinary Cytotoxicity Dichloromethane Disease transmission Exposure Female Flavonoids Fluorescence Fluorescence microscopy Helicteres velutina Hemocytes Hemolymph Immune system Insecticides - pharmacology Iodides Larva - drug effects Larva - growth & development Larvae larvicidal activity Larvicides Life Sciences & Biomedicine Malvaceae - chemistry Microscopy Morphology Mortality Mosquito Vectors - drug effects Mosquito Vectors - growth & development Mosquitoes Necrosis Nitric oxide Pest control Physical Sciences Plant Extracts - pharmacology Plant Leaves - chemistry Propidium iodide Public health Science & Technology Software Studies Sulfates tiliroside Viral diseases |
| title | Insights on the Larvicidal Mechanism of Action of Fractions and Compounds from Aerial Parts of Helicteres velutina K. Schum against Aedes aegypti L |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T18%3A11%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Insights%20on%20the%20Larvicidal%20Mechanism%20of%20Action%20of%20Fractions%20and%20Compounds%20from%20Aerial%20Parts%20of%20Helicteres%20velutina%20K.%20Schum%20against%20Aedes%20aegypti%20L&rft.jtitle=Molecules%20(Basel,%20Switzerland)&rft.au=Fernandes,%20Diegina%20A.&rft.date=2020-07-01&rft.volume=25&rft.issue=13&rft.spage=3015&rft.pages=3015-&rft.artnum=3015&rft.issn=1420-3049&rft.eissn=1420-3049&rft_id=info:doi/10.3390/molecules25133015&rft_dat=%3Cproquest_pubme%3E2420332834%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2420332834&rft_id=info:pmid/32630318&rft_doaj_id=oai_doaj_org_article_27c748ae0fb54c73b10095e761db71ba&rfr_iscdi=true |