Temporal Sampling of Enzymes from Live Cells by Localized Electroporation and Quantification of Activity by SAMDI Mass Spectrometry

Measuring changes in enzymatic activity over time from small numbers of cells remains a significant technical challenge. In this work, a method for sampling the cytoplasm of cells is introduced to extract enzymes and measure their activity at multiple time points. A microfluidic device, termed the l...

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Veröffentlicht in:	Small (Weinheim an der Bergstrasse, Germany) Germany), 2020-07, Vol.16 (26), p.e2000584-n/a
Hauptverfasser:	Mukherjee, Prithvijit, Berns, Eric J., Patino, Cesar A., Hakim Moully, Elamar, Chang, Lingqian, Nathamgari, S. Shiva P., Kessler, John A., Mrksich, Milan, Espinosa, Horacio D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Line, Tumor Cell membranes cell sampling Cells - enzymology Chambers Cytoplasm Electroporation enzyme activities Enzyme Assays - instrumentation Enzyme Assays - methods Enzymes Enzymes - analysis Enzymes - metabolism Humans Hydrogen peroxide Kinases Mass spectrometry Microfluidic devices microfluidics Nanochannels nanotechnologies Nanotechnology Oxidation Sampling Scientific imaging Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectroscopy Substrates Time
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