Lactobacillus helveticus -Fermented Milk Whey Suppresses Melanin Production by Inhibiting Tyrosinase through Decreasing MITF Expression
Whey obtained from milk fermented by the CM4 strain (LHMW) has been shown to improve skin barrier function and increase skin-moisturizing factors. In this study, we investigated the effects of LHMW on melanin production to explore the additional impacts of LHMW on the skin. We treated mouse B16 mela...
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| Veröffentlicht in: | Nutrients 2020-07, Vol.12 (7), p.2082 |
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| creator | Ikarashi, Nobutomo Fukuda, Natsuko Ochiai, Makiba Sasaki, Mami Kon, Risako Sakai, Hiroyasu Hatanaka, Misaki Kamei, Junzo |
| description | Whey obtained from milk fermented by the
CM4 strain (LHMW) has been shown to improve skin barrier function and increase skin-moisturizing factors. In this study, we investigated the effects of LHMW on melanin production to explore the additional impacts of LHMW on the skin. We treated mouse B16 melanoma cells with α-melanocyte-stimulating hormone (α-MSH) alone or simultaneously with LHMW and measured the amount of melanin. The amount of melanin in B16 cells treated with α-MSH significantly increased by 2-fold compared with that in control cells, and tyrosinase activity was also elevated. Moreover, treatment with LHMW significantly suppressed the increase in melanin content and elevation of tyrosinase activity due to α-MSH. LHMW also suppressed the α-MSH-induced increased expression of tyrosinase, tyrosinase-related protein 1 (TRP1), and dopachrome tautomerase (DCT) at the protein and mRNA levels. Furthermore, the mRNA and protein microphthalmia-associated transcription factor (MITF) expression levels were significantly increased with treatment with α-MSH alone, which were also suppressed by LHMW addition. LHMW suppression of melanin production is suggested to involve inhibition of the expression of the tyrosinase gene family by lowering the MITF expression level. LHMW may have promise as a material for cosmetics with expected clinical application in humans. |
| doi_str_mv | 10.3390/nu12072082 |
| format | Article |
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CM4 strain (LHMW) has been shown to improve skin barrier function and increase skin-moisturizing factors. In this study, we investigated the effects of LHMW on melanin production to explore the additional impacts of LHMW on the skin. We treated mouse B16 melanoma cells with α-melanocyte-stimulating hormone (α-MSH) alone or simultaneously with LHMW and measured the amount of melanin. The amount of melanin in B16 cells treated with α-MSH significantly increased by 2-fold compared with that in control cells, and tyrosinase activity was also elevated. Moreover, treatment with LHMW significantly suppressed the increase in melanin content and elevation of tyrosinase activity due to α-MSH. LHMW also suppressed the α-MSH-induced increased expression of tyrosinase, tyrosinase-related protein 1 (TRP1), and dopachrome tautomerase (DCT) at the protein and mRNA levels. Furthermore, the mRNA and protein microphthalmia-associated transcription factor (MITF) expression levels were significantly increased with treatment with α-MSH alone, which were also suppressed by LHMW addition. LHMW suppression of melanin production is suggested to involve inhibition of the expression of the tyrosinase gene family by lowering the MITF expression level. LHMW may have promise as a material for cosmetics with expected clinical application in humans.</description><identifier>ISSN: 2072-6643</identifier><identifier>EISSN: 2072-6643</identifier><identifier>DOI: 10.3390/nu12072082</identifier><identifier>PMID: 32674403</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>alpha-MSH - pharmacology ; Animals ; Antibodies ; Cell Line, Tumor ; Cosmetics ; Cultured Milk Products ; Dopachrome isomerase ; Enzymes ; Fermentation ; Fermented milk products ; Gene Expression ; Lactobacilli ; Lactobacillus helveticus ; Lactobacillus helveticus - metabolism ; Levels ; Melanin ; Melanins - biosynthesis ; Melanocyte-stimulating hormone ; Melanoma ; Melanoma, Experimental - metabolism ; Mice ; Microphthalmia-associated transcription factor ; Microphthalmia-Associated Transcription Factor - genetics ; Microphthalmia-Associated Transcription Factor - metabolism ; Milk ; Monophenol Monooxygenase - metabolism ; Proteins ; Skin ; Tyrosinase ; Tyrosinase gene ; Tyrosinase-related protein 1 ; Whey</subject><ispartof>Nutrients, 2020-07, Vol.12 (7), p.2082</ispartof><rights>2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-faf1da1fd2e4d0c392174dcf4be103ca470fe2277e97f37ff103558f14ea7aeb3</citedby><cites>FETCH-LOGICAL-c472t-faf1da1fd2e4d0c392174dcf4be103ca470fe2277e97f37ff103558f14ea7aeb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400678/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400678/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32674403$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ikarashi, Nobutomo</creatorcontrib><creatorcontrib>Fukuda, Natsuko</creatorcontrib><creatorcontrib>Ochiai, Makiba</creatorcontrib><creatorcontrib>Sasaki, Mami</creatorcontrib><creatorcontrib>Kon, Risako</creatorcontrib><creatorcontrib>Sakai, Hiroyasu</creatorcontrib><creatorcontrib>Hatanaka, Misaki</creatorcontrib><creatorcontrib>Kamei, Junzo</creatorcontrib><title>Lactobacillus helveticus -Fermented Milk Whey Suppresses Melanin Production by Inhibiting Tyrosinase through Decreasing MITF Expression</title><title>Nutrients</title><addtitle>Nutrients</addtitle><description>Whey obtained from milk fermented by the
CM4 strain (LHMW) has been shown to improve skin barrier function and increase skin-moisturizing factors. In this study, we investigated the effects of LHMW on melanin production to explore the additional impacts of LHMW on the skin. We treated mouse B16 melanoma cells with α-melanocyte-stimulating hormone (α-MSH) alone or simultaneously with LHMW and measured the amount of melanin. The amount of melanin in B16 cells treated with α-MSH significantly increased by 2-fold compared with that in control cells, and tyrosinase activity was also elevated. Moreover, treatment with LHMW significantly suppressed the increase in melanin content and elevation of tyrosinase activity due to α-MSH. LHMW also suppressed the α-MSH-induced increased expression of tyrosinase, tyrosinase-related protein 1 (TRP1), and dopachrome tautomerase (DCT) at the protein and mRNA levels. Furthermore, the mRNA and protein microphthalmia-associated transcription factor (MITF) expression levels were significantly increased with treatment with α-MSH alone, which were also suppressed by LHMW addition. LHMW suppression of melanin production is suggested to involve inhibition of the expression of the tyrosinase gene family by lowering the MITF expression level. LHMW may have promise as a material for cosmetics with expected clinical application in humans.</description><subject>alpha-MSH - pharmacology</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Cell Line, Tumor</subject><subject>Cosmetics</subject><subject>Cultured Milk Products</subject><subject>Dopachrome isomerase</subject><subject>Enzymes</subject><subject>Fermentation</subject><subject>Fermented milk products</subject><subject>Gene Expression</subject><subject>Lactobacilli</subject><subject>Lactobacillus helveticus</subject><subject>Lactobacillus helveticus - metabolism</subject><subject>Levels</subject><subject>Melanin</subject><subject>Melanins - biosynthesis</subject><subject>Melanocyte-stimulating hormone</subject><subject>Melanoma</subject><subject>Melanoma, Experimental - metabolism</subject><subject>Mice</subject><subject>Microphthalmia-associated transcription factor</subject><subject>Microphthalmia-Associated Transcription Factor - genetics</subject><subject>Microphthalmia-Associated Transcription Factor - metabolism</subject><subject>Milk</subject><subject>Monophenol Monooxygenase - metabolism</subject><subject>Proteins</subject><subject>Skin</subject><subject>Tyrosinase</subject><subject>Tyrosinase gene</subject><subject>Tyrosinase-related protein 1</subject><subject>Whey</subject><issn>2072-6643</issn><issn>2072-6643</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkd9qFDEUxoNYbNn2xgeQgDciTM2_TmZuBKldXdhFwRUvQyZzspM6m6zJpLhP4GubbWut5iYfOb98nHM-hJ5Tcs55S974TBmRjDTsCTo5qKquBX_6SB-js5SuyeFIImv-DB1zVkshCD9Bv5baTKHTxo1jTniA8QYmZ4qs5hC34Cfo8cqN3_G3Afb4S97tIqQECa9g1N55_DmGPpvJBY-7PV74wXVucn6D1_sYkvM6AZ6GGPJmwO_BRNDpUF0t1nN89fPWrfw9RUdWjwnO7u8Z-jq_Wl9-rJafPiwu3y0rIySbKqst7TW1PQPRE8NbRqXojRUdUMKNFpJYYExKaKXl0tryenHRWCpASw0dn6G3d7673G2hN2XAqEe1i26r414F7dS_Fe8GtQk3SgpCatkUg1f3BjH8yJAmtXXJwFiWASEnxQSnDeU15wV9-R96HXL0ZbxCMVG3bVPimaHXd5Qp60oR7EMzlKhDxOpvxAV-8bj9B_RPoPw3cUKk0g</recordid><startdate>20200714</startdate><enddate>20200714</enddate><creator>Ikarashi, Nobutomo</creator><creator>Fukuda, Natsuko</creator><creator>Ochiai, Makiba</creator><creator>Sasaki, Mami</creator><creator>Kon, Risako</creator><creator>Sakai, Hiroyasu</creator><creator>Hatanaka, Misaki</creator><creator>Kamei, Junzo</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200714</creationdate><title>Lactobacillus helveticus -Fermented Milk Whey Suppresses Melanin Production by Inhibiting Tyrosinase through Decreasing MITF Expression</title><author>Ikarashi, Nobutomo ; 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CM4 strain (LHMW) has been shown to improve skin barrier function and increase skin-moisturizing factors. In this study, we investigated the effects of LHMW on melanin production to explore the additional impacts of LHMW on the skin. We treated mouse B16 melanoma cells with α-melanocyte-stimulating hormone (α-MSH) alone or simultaneously with LHMW and measured the amount of melanin. The amount of melanin in B16 cells treated with α-MSH significantly increased by 2-fold compared with that in control cells, and tyrosinase activity was also elevated. Moreover, treatment with LHMW significantly suppressed the increase in melanin content and elevation of tyrosinase activity due to α-MSH. LHMW also suppressed the α-MSH-induced increased expression of tyrosinase, tyrosinase-related protein 1 (TRP1), and dopachrome tautomerase (DCT) at the protein and mRNA levels. Furthermore, the mRNA and protein microphthalmia-associated transcription factor (MITF) expression levels were significantly increased with treatment with α-MSH alone, which were also suppressed by LHMW addition. LHMW suppression of melanin production is suggested to involve inhibition of the expression of the tyrosinase gene family by lowering the MITF expression level. LHMW may have promise as a material for cosmetics with expected clinical application in humans.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>32674403</pmid><doi>10.3390/nu12072082</doi><oa>free_for_read</oa></addata></record> |
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| subjects | alpha-MSH - pharmacology Animals Antibodies Cell Line, Tumor Cosmetics Cultured Milk Products Dopachrome isomerase Enzymes Fermentation Fermented milk products Gene Expression Lactobacilli Lactobacillus helveticus Lactobacillus helveticus - metabolism Levels Melanin Melanins - biosynthesis Melanocyte-stimulating hormone Melanoma Melanoma, Experimental - metabolism Mice Microphthalmia-associated transcription factor Microphthalmia-Associated Transcription Factor - genetics Microphthalmia-Associated Transcription Factor - metabolism Milk Monophenol Monooxygenase - metabolism Proteins Skin Tyrosinase Tyrosinase gene Tyrosinase-related protein 1 Whey |
| title | Lactobacillus helveticus -Fermented Milk Whey Suppresses Melanin Production by Inhibiting Tyrosinase through Decreasing MITF Expression |
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