Selection of a malignant subpopulation from a colorectal cancer cell line
Colorectal cancer (CRC) is a leading cause of cancer-associated mortality worldwide; therefore, there is an emerging need for novel experimental models that allow for the identification and validation of biomarkers for CRC-specific progression. In the present study, a repeated sphere-forming assay w...
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| Veröffentlicht in: | Oncology letters 2020-09, Vol.20 (3), p.2937-2945 |
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| container_title | Oncology letters |
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| creator | Lai, Pei-Lun Chen, Ting-Chun Feng, Chun-Yen Lin, Hsuan Ng, Chi-Hou Chen, Yun Hsiao, Michael Lu, Jean Huang, Hsiao-Chun |
| description | Colorectal cancer (CRC) is a leading cause of cancer-associated mortality worldwide; therefore, there is an emerging need for novel experimental models that allow for the identification and validation of biomarkers for CRC-specific progression. In the present study, a repeated sphere-forming assay was used as a strategy to select a malignant subpopulation from a CRC cell line, namely HCT116. The assay was validated by confirming that canonical stemness markers were upregulated in the sphere state at every generation of the selection assay. The resulting subpopulation, after eight rounds of selection, exhibited increased sphere-forming capacity in vitro and increased tumorigenicity in vivo. Furthermore, dipeptidase 1 (DPEP1) was identified as the major differentially expressed gene in the selected clone, and its depletion suppressed the elevated sphere-forming capacity in vitro and tumorigenicity in vivo. Overall, the present study established an experimental strategy to isolate a malignant subpopulation from a CRC cell line. Additionally, results from the present model revealed that DPEP1 may serve as a promising prognostic biomarker for CRC. Key words: cell line, colorectal cancer, DPEP1, malignant subpopulation, sphere-forming |
| doi_str_mv | 10.3892/ol.2020.11829 |
| format | Article |
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In the present study, a repeated sphere-forming assay was used as a strategy to select a malignant subpopulation from a CRC cell line, namely HCT116. The assay was validated by confirming that canonical stemness markers were upregulated in the sphere state at every generation of the selection assay. The resulting subpopulation, after eight rounds of selection, exhibited increased sphere-forming capacity in vitro and increased tumorigenicity in vivo. Furthermore, dipeptidase 1 (DPEP1) was identified as the major differentially expressed gene in the selected clone, and its depletion suppressed the elevated sphere-forming capacity in vitro and tumorigenicity in vivo. Overall, the present study established an experimental strategy to isolate a malignant subpopulation from a CRC cell line. Additionally, results from the present model revealed that DPEP1 may serve as a promising prognostic biomarker for CRC. Key words: cell line, colorectal cancer, DPEP1, malignant subpopulation, sphere-forming</description><identifier>ISSN: 1792-1074</identifier><identifier>EISSN: 1792-1082</identifier><identifier>DOI: 10.3892/ol.2020.11829</identifier><identifier>PMID: 32782610</identifier><language>eng</language><publisher>Athens: Spandidos Publications</publisher><subject>Analysis ; Cloning ; Colorectal cancer ; Gene expression ; Growth factors ; Health aspects ; Laboratories ; Metastasis ; Oncology ; Proteins ; Stem cells</subject><ispartof>Oncology letters, 2020-09, Vol.20 (3), p.2937-2945</ispartof><rights>COPYRIGHT 2020 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2020</rights><rights>Copyright: © Lai et al. 2020</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-f6572ecaf0a6b57f307c641053ba428509dec57c8df21efe4db73d1872525f823</citedby><cites>FETCH-LOGICAL-c490t-f6572ecaf0a6b57f307c641053ba428509dec57c8df21efe4db73d1872525f823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399770/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399770/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids></links><search><creatorcontrib>Lai, Pei-Lun</creatorcontrib><creatorcontrib>Chen, Ting-Chun</creatorcontrib><creatorcontrib>Feng, Chun-Yen</creatorcontrib><creatorcontrib>Lin, Hsuan</creatorcontrib><creatorcontrib>Ng, Chi-Hou</creatorcontrib><creatorcontrib>Chen, Yun</creatorcontrib><creatorcontrib>Hsiao, Michael</creatorcontrib><creatorcontrib>Lu, Jean</creatorcontrib><creatorcontrib>Huang, Hsiao-Chun</creatorcontrib><title>Selection of a malignant subpopulation from a colorectal cancer cell line</title><title>Oncology letters</title><description>Colorectal cancer (CRC) is a leading cause of cancer-associated mortality worldwide; therefore, there is an emerging need for novel experimental models that allow for the identification and validation of biomarkers for CRC-specific progression. In the present study, a repeated sphere-forming assay was used as a strategy to select a malignant subpopulation from a CRC cell line, namely HCT116. The assay was validated by confirming that canonical stemness markers were upregulated in the sphere state at every generation of the selection assay. The resulting subpopulation, after eight rounds of selection, exhibited increased sphere-forming capacity in vitro and increased tumorigenicity in vivo. Furthermore, dipeptidase 1 (DPEP1) was identified as the major differentially expressed gene in the selected clone, and its depletion suppressed the elevated sphere-forming capacity in vitro and tumorigenicity in vivo. Overall, the present study established an experimental strategy to isolate a malignant subpopulation from a CRC cell line. Additionally, results from the present model revealed that DPEP1 may serve as a promising prognostic biomarker for CRC. 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In the present study, a repeated sphere-forming assay was used as a strategy to select a malignant subpopulation from a CRC cell line, namely HCT116. The assay was validated by confirming that canonical stemness markers were upregulated in the sphere state at every generation of the selection assay. The resulting subpopulation, after eight rounds of selection, exhibited increased sphere-forming capacity in vitro and increased tumorigenicity in vivo. Furthermore, dipeptidase 1 (DPEP1) was identified as the major differentially expressed gene in the selected clone, and its depletion suppressed the elevated sphere-forming capacity in vitro and tumorigenicity in vivo. Overall, the present study established an experimental strategy to isolate a malignant subpopulation from a CRC cell line. Additionally, results from the present model revealed that DPEP1 may serve as a promising prognostic biomarker for CRC. 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| source | Spandidos Publications Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Analysis Cloning Colorectal cancer Gene expression Growth factors Health aspects Laboratories Metastasis Oncology Proteins Stem cells |
| title | Selection of a malignant subpopulation from a colorectal cancer cell line |
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