Combined Selective Plane Illumination Microscopy and FRAP Maps Intranuclear Diffusion of NLS-GFP

Since its initial development in 1976, fluorescence recovery after photobleaching (FRAP) has been one of the most popular tools for studying diffusion and protein dynamics in living cells. Its popularity is derived from the widespread availability of confocal microscopes and the relative ease of the...

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Veröffentlicht in:Biophysical journal 2020-08, Vol.119 (3), p.514-524
Hauptverfasser: Hobson, Chad M., O’Brien, E. Timothy, Falvo, Michael R., Superfine, Richard
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Sprache:eng
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