Simple, sensitive and specific quantification of diamine oxidase activity in complex matrices using newly discovered fluorophores derived from natural substrates

Objective To measure diamine oxidase (DAO) activity with high sensitivity in complex matrices like plasma or tissue extracts radioactive putrescine or horseradish peroxidase (HRP)/hydrogen peroxide (H 2 O 2 ) coupling must be used. The use of radioactive material should be avoided and HRP/H 2 O 2 co...

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Veröffentlicht in:	Inflammation research 2020-09, Vol.69 (9), p.937-950
Hauptverfasser:	Boehm, Thomas, Karer, Matthias, Gludovacz, Elisabeth, Petroczi, Karin, Resch, Marlene, Schuetzenberger, Kornelia, Klavins, Kristaps, Borth, Nicole, Jilma, Bernd
Format:	Artikel
Sprache:	eng
Schlagworte:	Absorption Allergology Antioxidants Biomedical and Life Sciences Biomedicine Blood plasma Cadaverine Chemical compounds Condensates Coupling DELTA protein Dermatology Detection limits Diamines Enzyme-linked immunosorbent assay Fluorescence Fluorophores Horseradish peroxidase Hydrogen peroxide Immunology Intravenous administration Neurology Original Original Research Article Oxidase Peroxidase Pharmacology/Toxicology Plasma Pregnancy Putrescine Radioactive materials Rheumatology Spiking Substrates Tissues
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