Evaluation of In Vivo Antidiabetic, In Vitro α-Amylase Inhibitory, and In Vitro Antioxidant Activity of Leaves Crude Extract and Solvent Fractions of Bersama abyssinica Fresen (Melianthaceae)
Background. The leaves of Bersama abyssinica are used for the treatment of diabetes mellitus in folk medicine system of Ethiopia. The present study was done based on the traditional claim of B abyssinica for the treatment of diabetes mellitus. Methods. The α-amylase inhibition and antioxidant activi...
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| Veröffentlicht in: | Journal of evidence-based integrative medicine 2020, Vol.25, p.2515690X20935827-2515690X20935827 |
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| creator | Kifle, Zemene Demelash Enyew, Engidaw Fentahun |
| description | Background.
The leaves of Bersama abyssinica are used for the treatment of diabetes mellitus in folk medicine system of Ethiopia. The present study was done based on the traditional claim of B abyssinica for the treatment of diabetes mellitus.
Methods.
The α-amylase inhibition and antioxidant activities of B abyssinica extracts were evaluated by using 3,5-dinitrosalicylic acid method and diphenyl-2-picrylhydrazyl assay model, respectively. Blood glucose lowering activity of the extracts was studied in 4 animal models; normoglycemic, oral glucose loaded, and streptozotocin-induced diabetic mice models.
Results.
Among the extracts, the crude extract showed the highest α-amylase enzyme inhibition activity with an IC50 of 6.57 μg/mL. The water fraction showed the strongest antioxidant activity with an IC50 of 3.43 μg/mL. The crude extract at doses of 200, and 400 mg/kg showed significant (P < .05) hypoglycemic activity in normoglycemic mice. All doses of the crude extract significantly (P < .05) reduced blood glucose levels of oral glucose-loaded mice. In streptozotocin-induced diabetic mice models, both the crude and solvent fractions showed a significant (P < .05) blood glucose lowering effect as compared with the negative control group post 8 hour treatment.
Conclusion.
The results demonstrated the beneficial biochemical effects of B abyssinica extract by inhibiting α-amylase and scavenging the free radicals. The crude extract and solvent fractions of B abyssinica had significant blood glucose lowering effect in all animal models. |
| doi_str_mv | 10.1177/2515690X20935827 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7388106</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_2515690X20935827</sage_id><sourcerecordid>2473692663</sourcerecordid><originalsourceid>FETCH-LOGICAL-c420t-1899d0cc219b40be0b844f10e199636a00a25bc5049fcc9823d4f336f1a7b7f13</originalsourceid><addsrcrecordid>eNp1kstu1TAQhiMEolXpnhWyxKZIDfiSmzdIh6NTqHQQCy5iF02cSY-rHLu1nah5LN6AJ-gz1eGUtlRiZeuf7_9nRpokecnoW8bK8h3PWV5I-pNTKfKKl0-S_VlKZ-3pg_9ecuj9OaWUyUpmefE82RO8ZFXM2E9-r0boBwjaGmI7cmrIDz1asjBBtxoaDFod79TgLLn-lS62Uw8eo7bRjQ7WTccETHvPzFZ7pVswgSxU0KMO0xy9RhjRk6UbWiSrq-BAhT_Or7YfMcInsxLn8DP9AZ2HLRBoJu-10QpiHT0acvQZex3DN6AQ8M2L5FkHvcfD2_cg-X6y-rb8lK6_fDxdLtapyjgNKaukbKlSnMkmow3SpsqyjlFkUhaiAEqB543KaSY7pWTFRZt1QhQdg7IpOyYOkve73Iuh2WKr4sQO-vrC6S24qbag638rRm_qMzvWpagqRosYcHQb4OzlgD7UW-0V9j0YtIOvecariDEhIvr6EXpuB2fiepEqRSF5UcwU3VHKWe8ddnfDMFrPF1I_vpBoefVwiTvD33uIQLoDPJzhfdf_Bt4AcRrGdQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2473692663</pqid></control><display><type>article</type><title>Evaluation of In Vivo Antidiabetic, In Vitro α-Amylase Inhibitory, and In Vitro Antioxidant Activity of Leaves Crude Extract and Solvent Fractions of Bersama abyssinica Fresen (Melianthaceae)</title><source>Applied Social Sciences Index & Abstracts (ASSIA)</source><source>MEDLINE</source><source>SAGE Open Access</source><source>PubMed Central</source><source>Directory of Open Access Journals</source><creator>Kifle, Zemene Demelash ; Enyew, Engidaw Fentahun</creator><creatorcontrib>Kifle, Zemene Demelash ; Enyew, Engidaw Fentahun</creatorcontrib><description>Background.
The leaves of Bersama abyssinica are used for the treatment of diabetes mellitus in folk medicine system of Ethiopia. The present study was done based on the traditional claim of B abyssinica for the treatment of diabetes mellitus.
Methods.
The α-amylase inhibition and antioxidant activities of B abyssinica extracts were evaluated by using 3,5-dinitrosalicylic acid method and diphenyl-2-picrylhydrazyl assay model, respectively. Blood glucose lowering activity of the extracts was studied in 4 animal models; normoglycemic, oral glucose loaded, and streptozotocin-induced diabetic mice models.
Results.
Among the extracts, the crude extract showed the highest α-amylase enzyme inhibition activity with an IC50 of 6.57 μg/mL. The water fraction showed the strongest antioxidant activity with an IC50 of 3.43 μg/mL. The crude extract at doses of 200, and 400 mg/kg showed significant (P < .05) hypoglycemic activity in normoglycemic mice. All doses of the crude extract significantly (P < .05) reduced blood glucose levels of oral glucose-loaded mice. In streptozotocin-induced diabetic mice models, both the crude and solvent fractions showed a significant (P < .05) blood glucose lowering effect as compared with the negative control group post 8 hour treatment.
Conclusion.
The results demonstrated the beneficial biochemical effects of B abyssinica extract by inhibiting α-amylase and scavenging the free radicals. The crude extract and solvent fractions of B abyssinica had significant blood glucose lowering effect in all animal models.</description><identifier>ISSN: 2515-690X</identifier><identifier>EISSN: 2515-690X</identifier><identifier>DOI: 10.1177/2515690X20935827</identifier><identifier>PMID: 32718177</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>alpha-Amylases - antagonists & inhibitors ; Animals ; Antidiabetics ; Antioxidants ; Antioxidants - pharmacology ; Blood ; Blood Glucose - drug effects ; Diabetes ; Diabetes Mellitus, Experimental - drug therapy ; Disease Models, Animal ; Dosage ; Ethiopia ; Glucose ; Herbal medicine ; Hypoglycemic Agents - pharmacology ; Inhibition ; Male ; Mice ; Original ; Plant Extracts - pharmacology ; Plant Leaves ; Solvents ; Streptozocin</subject><ispartof>Journal of evidence-based integrative medicine, 2020, Vol.25, p.2515690X20935827-2515690X20935827</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. This work is licensed under the Creative Commons Attribution – Non-Commercial License https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2020 2020 SAGE Publications Inc unless otherwise noted. Manuscript content on this site is licensed under Creative Commons Licenses</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-1899d0cc219b40be0b844f10e199636a00a25bc5049fcc9823d4f336f1a7b7f13</citedby><cites>FETCH-LOGICAL-c420t-1899d0cc219b40be0b844f10e199636a00a25bc5049fcc9823d4f336f1a7b7f13</cites><orcidid>0000-0001-7030-2782</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388106/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388106/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,4024,12846,21966,27853,27923,27924,27925,30999,44945,45333,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32718177$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kifle, Zemene Demelash</creatorcontrib><creatorcontrib>Enyew, Engidaw Fentahun</creatorcontrib><title>Evaluation of In Vivo Antidiabetic, In Vitro α-Amylase Inhibitory, and In Vitro Antioxidant Activity of Leaves Crude Extract and Solvent Fractions of Bersama abyssinica Fresen (Melianthaceae)</title><title>Journal of evidence-based integrative medicine</title><addtitle>J Evid Based Integr Med</addtitle><description>Background.
The leaves of Bersama abyssinica are used for the treatment of diabetes mellitus in folk medicine system of Ethiopia. The present study was done based on the traditional claim of B abyssinica for the treatment of diabetes mellitus.
Methods.
The α-amylase inhibition and antioxidant activities of B abyssinica extracts were evaluated by using 3,5-dinitrosalicylic acid method and diphenyl-2-picrylhydrazyl assay model, respectively. Blood glucose lowering activity of the extracts was studied in 4 animal models; normoglycemic, oral glucose loaded, and streptozotocin-induced diabetic mice models.
Results.
Among the extracts, the crude extract showed the highest α-amylase enzyme inhibition activity with an IC50 of 6.57 μg/mL. The water fraction showed the strongest antioxidant activity with an IC50 of 3.43 μg/mL. The crude extract at doses of 200, and 400 mg/kg showed significant (P < .05) hypoglycemic activity in normoglycemic mice. All doses of the crude extract significantly (P < .05) reduced blood glucose levels of oral glucose-loaded mice. In streptozotocin-induced diabetic mice models, both the crude and solvent fractions showed a significant (P < .05) blood glucose lowering effect as compared with the negative control group post 8 hour treatment.
Conclusion.
The results demonstrated the beneficial biochemical effects of B abyssinica extract by inhibiting α-amylase and scavenging the free radicals. The crude extract and solvent fractions of B abyssinica had significant blood glucose lowering effect in all animal models.</description><subject>alpha-Amylases - antagonists & inhibitors</subject><subject>Animals</subject><subject>Antidiabetics</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Blood</subject><subject>Blood Glucose - drug effects</subject><subject>Diabetes</subject><subject>Diabetes Mellitus, Experimental - drug therapy</subject><subject>Disease Models, Animal</subject><subject>Dosage</subject><subject>Ethiopia</subject><subject>Glucose</subject><subject>Herbal medicine</subject><subject>Hypoglycemic Agents - pharmacology</subject><subject>Inhibition</subject><subject>Male</subject><subject>Mice</subject><subject>Original</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves</subject><subject>Solvents</subject><subject>Streptozocin</subject><issn>2515-690X</issn><issn>2515-690X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AFRWT</sourceid><sourceid>EIF</sourceid><sourceid>7QJ</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kstu1TAQhiMEolXpnhWyxKZIDfiSmzdIh6NTqHQQCy5iF02cSY-rHLu1nah5LN6AJ-gz1eGUtlRiZeuf7_9nRpokecnoW8bK8h3PWV5I-pNTKfKKl0-S_VlKZ-3pg_9ecuj9OaWUyUpmefE82RO8ZFXM2E9-r0boBwjaGmI7cmrIDz1asjBBtxoaDFod79TgLLn-lS62Uw8eo7bRjQ7WTccETHvPzFZ7pVswgSxU0KMO0xy9RhjRk6UbWiSrq-BAhT_Or7YfMcInsxLn8DP9AZ2HLRBoJu-10QpiHT0acvQZex3DN6AQ8M2L5FkHvcfD2_cg-X6y-rb8lK6_fDxdLtapyjgNKaukbKlSnMkmow3SpsqyjlFkUhaiAEqB543KaSY7pWTFRZt1QhQdg7IpOyYOkve73Iuh2WKr4sQO-vrC6S24qbag638rRm_qMzvWpagqRosYcHQb4OzlgD7UW-0V9j0YtIOvecariDEhIvr6EXpuB2fiepEqRSF5UcwU3VHKWe8ddnfDMFrPF1I_vpBoefVwiTvD33uIQLoDPJzhfdf_Bt4AcRrGdQ</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Kifle, Zemene Demelash</creator><creator>Enyew, Engidaw Fentahun</creator><general>SAGE Publications</general><general>SAGE PUBLICATIONS, INC</general><scope>AFRWT</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QJ</scope><scope>7RV</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>KB0</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7030-2782</orcidid></search><sort><creationdate>2020</creationdate><title>Evaluation of In Vivo Antidiabetic, In Vitro α-Amylase Inhibitory, and In Vitro Antioxidant Activity of Leaves Crude Extract and Solvent Fractions of Bersama abyssinica Fresen (Melianthaceae)</title><author>Kifle, Zemene Demelash ; Enyew, Engidaw Fentahun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-1899d0cc219b40be0b844f10e199636a00a25bc5049fcc9823d4f336f1a7b7f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>alpha-Amylases - antagonists & inhibitors</topic><topic>Animals</topic><topic>Antidiabetics</topic><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Blood</topic><topic>Blood Glucose - drug effects</topic><topic>Diabetes</topic><topic>Diabetes Mellitus, Experimental - drug therapy</topic><topic>Disease Models, Animal</topic><topic>Dosage</topic><topic>Ethiopia</topic><topic>Glucose</topic><topic>Herbal medicine</topic><topic>Hypoglycemic Agents - pharmacology</topic><topic>Inhibition</topic><topic>Male</topic><topic>Mice</topic><topic>Original</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves</topic><topic>Solvents</topic><topic>Streptozocin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kifle, Zemene Demelash</creatorcontrib><creatorcontrib>Enyew, Engidaw Fentahun</creatorcontrib><collection>SAGE Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Applied Social Sciences Index & Abstracts (ASSIA)</collection><collection>ProQuest Nursing & Allied Health Database</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of evidence-based integrative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kifle, Zemene Demelash</au><au>Enyew, Engidaw Fentahun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of In Vivo Antidiabetic, In Vitro α-Amylase Inhibitory, and In Vitro Antioxidant Activity of Leaves Crude Extract and Solvent Fractions of Bersama abyssinica Fresen (Melianthaceae)</atitle><jtitle>Journal of evidence-based integrative medicine</jtitle><addtitle>J Evid Based Integr Med</addtitle><date>2020</date><risdate>2020</risdate><volume>25</volume><spage>2515690X20935827</spage><epage>2515690X20935827</epage><pages>2515690X20935827-2515690X20935827</pages><issn>2515-690X</issn><eissn>2515-690X</eissn><abstract>Background.
The leaves of Bersama abyssinica are used for the treatment of diabetes mellitus in folk medicine system of Ethiopia. The present study was done based on the traditional claim of B abyssinica for the treatment of diabetes mellitus.
Methods.
The α-amylase inhibition and antioxidant activities of B abyssinica extracts were evaluated by using 3,5-dinitrosalicylic acid method and diphenyl-2-picrylhydrazyl assay model, respectively. Blood glucose lowering activity of the extracts was studied in 4 animal models; normoglycemic, oral glucose loaded, and streptozotocin-induced diabetic mice models.
Results.
Among the extracts, the crude extract showed the highest α-amylase enzyme inhibition activity with an IC50 of 6.57 μg/mL. The water fraction showed the strongest antioxidant activity with an IC50 of 3.43 μg/mL. The crude extract at doses of 200, and 400 mg/kg showed significant (P < .05) hypoglycemic activity in normoglycemic mice. All doses of the crude extract significantly (P < .05) reduced blood glucose levels of oral glucose-loaded mice. In streptozotocin-induced diabetic mice models, both the crude and solvent fractions showed a significant (P < .05) blood glucose lowering effect as compared with the negative control group post 8 hour treatment.
Conclusion.
The results demonstrated the beneficial biochemical effects of B abyssinica extract by inhibiting α-amylase and scavenging the free radicals. The crude extract and solvent fractions of B abyssinica had significant blood glucose lowering effect in all animal models.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>32718177</pmid><doi>10.1177/2515690X20935827</doi><orcidid>https://orcid.org/0000-0001-7030-2782</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | alpha-Amylases - antagonists & inhibitors Animals Antidiabetics Antioxidants Antioxidants - pharmacology Blood Blood Glucose - drug effects Diabetes Diabetes Mellitus, Experimental - drug therapy Disease Models, Animal Dosage Ethiopia Glucose Herbal medicine Hypoglycemic Agents - pharmacology Inhibition Male Mice Original Plant Extracts - pharmacology Plant Leaves Solvents Streptozocin |
| title | Evaluation of In Vivo Antidiabetic, In Vitro α-Amylase Inhibitory, and In Vitro Antioxidant Activity of Leaves Crude Extract and Solvent Fractions of Bersama abyssinica Fresen (Melianthaceae) |
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