Accelerated cryo-EM-guided determination of three-dimensional RNA-only structures

The discovery and design of biologically important RNA molecules is outpacing three-dimensional structural characterization. Here, we demonstrate that cryo-electron microscopy can routinely resolve maps of RNA-only systems and that these maps enable subnanometer-resolution coordinate estimation when...

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Veröffentlicht in:	Nature methods 2020-07, Vol.17 (7), p.699-707
Hauptverfasser:	Kappel, Kalli, Zhang, Kaiming, Su, Zhaoming, Watkins, Andrew M., Kladwang, Wipapat, Li, Shanshan, Pintilie, Grigore, Topkar, Ved V., Rangan, Ramya, Zheludev, Ivan N., Yesselman, Joseph D., Chiu, Wah, Das, Rhiju
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2258/1258/1259 631/45/500 631/57/2266 Accelerated tests Accuracy Adenosine triphosphate Adenosylmethionine AMP Aptamers BASIC BIOLOGICAL SCIENCES Benchmarks Biochemical analysis Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Catalytic RNA Cholera toxin Computational biophysics Computer applications Computer Simulation Computer-generated environments cryoelectron microscopy Cryoelectron Microscopy - methods Crystallography Design Electron microscopy Gene expression Glycine Homology Laboratories Life Sciences Ligases Mapping Microscopy Models, Molecular Mutagenesis Nucleic Acid Conformation Nucleotides Peptide mapping Proteins Proteomics Ribonucleic acid Riboswitch RNA RNA - chemistry RNA polymerase RNA, Catalytic - chemistry S-Adenosylmethionine Structural analysis Substrates Three dimensional analysis Three dimensional models Waterborne diseases
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