An Investigation into the Rehabilitative Mechanism of Tuina in the Treatment of Sciatic Nerve Injury
Objective. To explore the effect of tuina on the gene expression at the point of nerve injury in rats with sciatic nerve injury (SNI) and to elucidate the repair mechanism of tuina promoting the functional recovery of peripheral nerve injury. Methods. In the Sham group, the right sciatic nerve was e...
Gespeichert in:
| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2020, Vol.2020 (2020), p.1-11 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 11 |
|---|---|
| container_issue | 2020 |
| container_start_page | 1 |
| container_title | Evidence-based complementary and alternative medicine |
| container_volume | 2020 |
| creator | Wong, Steven Lu, MengQian Luo, Yuting Shao, Shuai Zhang, Yumo Yu, TianYuan Mo, Yanjun Lv, TaoTao Shen, Yi |
| description | Objective. To explore the effect of tuina on the gene expression at the point of nerve injury in rats with sciatic nerve injury (SNI) and to elucidate the repair mechanism of tuina promoting the functional recovery of peripheral nerve injury. Methods. In the Sham group, the right sciatic nerve was exposed without clamping. The SNI model was established using the sciatic nerve clamp method on the right leg and then randomly divided into the SNI group and the Tuina group. Seven days after modeling, the Tuina group was treated daily with a “massage and tuina manipulation simulator” (Patent No. ZL 2007 0187403.1), which was used daily to stimulate Yinmen (BL37), Yanglingquan (GB34), and Chengshan (BL57) with point-pressing method, plucking method, and kneading method. The stimulating force was 4N, and the stimulating frequency was 60 times per minute; each method and each point were used for 1 minute, totaling 9 minutes (1 min/acupoint/method × 3 methods × 3 acupoints). Treatment was administered for 21 days, followed by a 1-day rest after the 10th treatment, for a total of 20 times of intervention. The sciatic function index (SFI) was used to evaluate the fine movements of the hind limbs of rats in each group. The ultrastructural changes at the point of nerve injury were observed by transmission electron microscopy, and the gene changes at the point of nerve injury were detected using RNA-sequencing (RNA-seq) technology. Results. Compared with the baseline, the SFI of the SNI group and the Tuina group decreased significantly at the 0th intervention (7 days after molding); compared with the SNI group, the SFI of the Tuina group increased at the 10th intervention (P |
| doi_str_mv | 10.1155/2020/5859298 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7382723</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A639548469</galeid><sourcerecordid>A639548469</sourcerecordid><originalsourceid>FETCH-LOGICAL-c499t-6808c4d227e130ac6a34ae955296b6898c905ae924d66440e2058730cd5b686a3</originalsourceid><addsrcrecordid>eNqNkcuP0zAQxiMEYh9w44wicUFaytqOnxekasWj0gISFImb5TqTxlVq7zpJ0f73TGjpAidOtmZ-_jzzfUXxjJLXlApxyQgjl0ILw4x-UJxSxemMM60fHu_q-0lx1vcbQphRSj0uTiqmGK-YPC3qeSwXcQf9ENZuCCmWIQ6pHFoov0DrVqELA9Z3UH4E37oY-m2ZmnI5hugQ_QUuM7hhC3GYOl99QN6XnyDjo0XcjPnuSfGocV0PTw_nefHt3dvl1YfZ9ef3i6v59cxzY4aZ1ER7XjOmgFbEeekq7sAIwYxcSW20N0RggfFaSs4JMCK0qoivBbaRPi_e7HVvxtUWao8jZdfZmxy2Lt_Z5IL9uxNDa9dpZ1Wl0ZEKBV4eBHK6HdEUuw29h65zEdLYW4bGcqqU1oi--AfdpDFHXG-iFKMGTb6n1q4DG2KT8F8_idq5rIzgmkuD1Ks95XPq-wzNcWRK7BSynUK2h5ARf_7nmkf4d6oIXOyBNsTa_Qj_KQfIQOPuaSqkkqT6CY7ptyM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2427219327</pqid></control><display><type>article</type><title>An Investigation into the Rehabilitative Mechanism of Tuina in the Treatment of Sciatic Nerve Injury</title><source>PubMed Central Open Access</source><source>Wiley-Blackwell Open Access Titles</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Wong, Steven ; Lu, MengQian ; Luo, Yuting ; Shao, Shuai ; Zhang, Yumo ; Yu, TianYuan ; Mo, Yanjun ; Lv, TaoTao ; Shen, Yi</creator><contributor>Duann, Jeng-Ren ; Jeng-Ren Duann</contributor><creatorcontrib>Wong, Steven ; Lu, MengQian ; Luo, Yuting ; Shao, Shuai ; Zhang, Yumo ; Yu, TianYuan ; Mo, Yanjun ; Lv, TaoTao ; Shen, Yi ; Duann, Jeng-Ren ; Jeng-Ren Duann</creatorcontrib><description>Objective. To explore the effect of tuina on the gene expression at the point of nerve injury in rats with sciatic nerve injury (SNI) and to elucidate the repair mechanism of tuina promoting the functional recovery of peripheral nerve injury. Methods. In the Sham group, the right sciatic nerve was exposed without clamping. The SNI model was established using the sciatic nerve clamp method on the right leg and then randomly divided into the SNI group and the Tuina group. Seven days after modeling, the Tuina group was treated daily with a “massage and tuina manipulation simulator” (Patent No. ZL 2007 0187403.1), which was used daily to stimulate Yinmen (BL37), Yanglingquan (GB34), and Chengshan (BL57) with point-pressing method, plucking method, and kneading method. The stimulating force was 4N, and the stimulating frequency was 60 times per minute; each method and each point were used for 1 minute, totaling 9 minutes (1 min/acupoint/method × 3 methods × 3 acupoints). Treatment was administered for 21 days, followed by a 1-day rest after the 10th treatment, for a total of 20 times of intervention. The sciatic function index (SFI) was used to evaluate the fine movements of the hind limbs of rats in each group. The ultrastructural changes at the point of nerve injury were observed by transmission electron microscopy, and the gene changes at the point of nerve injury were detected using RNA-sequencing (RNA-seq) technology. Results. Compared with the baseline, the SFI of the SNI group and the Tuina group decreased significantly at the 0th intervention (7 days after molding); compared with the SNI group, the SFI of the Tuina group increased at the 10th intervention (P<0.05) and increased significantly at the 15th and 20th intervention (P<0.01). Compared with the Sham group, the myelin sheath integrity of the sciatic nerve in the SNI group was destroyed and the myelin sheath collapsed seriously, even forming myelin sheath ball, accompanied with severe axonal atrophy and mitochondrial degeneration. The tuina intervention could significantly improve the ultrastructure of the nerve injury point, and the nerve fiber myelin sheath in the Tuina group remained intact, without obvious axonal swelling or atrophy. Atrophic thread granules could be seen in the axon, but there were no vacuolated mitochondria. RNA-seq results showed that there were differences at 221 genes at the point of nerve injury between the Tuina group and the SNI group and the differentially expressed genes (DEGs) are enriched in the biological processes related to the regulation of myocyte. Regulations include the regulation of striated muscle cell differentiation, myoblast differentiation, and myotube differentiation. Conclusion. Tuina can improve the fine motor recovery and protect the myelin integrity in rats with peripheral nerve injury, and this is achieved by changing the gene sequence at the injured point.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2020/5859298</identifier><identifier>PMID: 32724326</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Acupuncture ; Analysis ; Animals ; Atrophy ; Cell differentiation ; Chinese medicine ; Gene expression ; Intervention ; Investigations ; Methods ; Microscopy ; Mitochondria ; Myelin ; Myoblasts ; Myocytes ; Myotubes ; Neurodegeneration ; Peripheral nerves ; Recovery of function ; Ribonucleic acid ; RNA ; Sciatic nerve ; Skeletal muscle ; Skin ; Transmission electron microscopy ; Ultrastructure</subject><ispartof>Evidence-based complementary and alternative medicine, 2020, Vol.2020 (2020), p.1-11</ispartof><rights>Copyright © 2020 Taotao Lv et al.</rights><rights>COPYRIGHT 2020 John Wiley & Sons, Inc.</rights><rights>Copyright © 2020 Taotao Lv et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2020 Taotao Lv et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-6808c4d227e130ac6a34ae955296b6898c905ae924d66440e2058730cd5b686a3</citedby><cites>FETCH-LOGICAL-c499t-6808c4d227e130ac6a34ae955296b6898c905ae924d66440e2058730cd5b686a3</cites><orcidid>0000-0001-6838-3851 ; 0000-0003-2089-3101</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382723/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382723/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4021,27921,27922,27923,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32724326$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Duann, Jeng-Ren</contributor><contributor>Jeng-Ren Duann</contributor><creatorcontrib>Wong, Steven</creatorcontrib><creatorcontrib>Lu, MengQian</creatorcontrib><creatorcontrib>Luo, Yuting</creatorcontrib><creatorcontrib>Shao, Shuai</creatorcontrib><creatorcontrib>Zhang, Yumo</creatorcontrib><creatorcontrib>Yu, TianYuan</creatorcontrib><creatorcontrib>Mo, Yanjun</creatorcontrib><creatorcontrib>Lv, TaoTao</creatorcontrib><creatorcontrib>Shen, Yi</creatorcontrib><title>An Investigation into the Rehabilitative Mechanism of Tuina in the Treatment of Sciatic Nerve Injury</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Objective. To explore the effect of tuina on the gene expression at the point of nerve injury in rats with sciatic nerve injury (SNI) and to elucidate the repair mechanism of tuina promoting the functional recovery of peripheral nerve injury. Methods. In the Sham group, the right sciatic nerve was exposed without clamping. The SNI model was established using the sciatic nerve clamp method on the right leg and then randomly divided into the SNI group and the Tuina group. Seven days after modeling, the Tuina group was treated daily with a “massage and tuina manipulation simulator” (Patent No. ZL 2007 0187403.1), which was used daily to stimulate Yinmen (BL37), Yanglingquan (GB34), and Chengshan (BL57) with point-pressing method, plucking method, and kneading method. The stimulating force was 4N, and the stimulating frequency was 60 times per minute; each method and each point were used for 1 minute, totaling 9 minutes (1 min/acupoint/method × 3 methods × 3 acupoints). Treatment was administered for 21 days, followed by a 1-day rest after the 10th treatment, for a total of 20 times of intervention. The sciatic function index (SFI) was used to evaluate the fine movements of the hind limbs of rats in each group. The ultrastructural changes at the point of nerve injury were observed by transmission electron microscopy, and the gene changes at the point of nerve injury were detected using RNA-sequencing (RNA-seq) technology. Results. Compared with the baseline, the SFI of the SNI group and the Tuina group decreased significantly at the 0th intervention (7 days after molding); compared with the SNI group, the SFI of the Tuina group increased at the 10th intervention (P<0.05) and increased significantly at the 15th and 20th intervention (P<0.01). Compared with the Sham group, the myelin sheath integrity of the sciatic nerve in the SNI group was destroyed and the myelin sheath collapsed seriously, even forming myelin sheath ball, accompanied with severe axonal atrophy and mitochondrial degeneration. The tuina intervention could significantly improve the ultrastructure of the nerve injury point, and the nerve fiber myelin sheath in the Tuina group remained intact, without obvious axonal swelling or atrophy. Atrophic thread granules could be seen in the axon, but there were no vacuolated mitochondria. RNA-seq results showed that there were differences at 221 genes at the point of nerve injury between the Tuina group and the SNI group and the differentially expressed genes (DEGs) are enriched in the biological processes related to the regulation of myocyte. Regulations include the regulation of striated muscle cell differentiation, myoblast differentiation, and myotube differentiation. Conclusion. Tuina can improve the fine motor recovery and protect the myelin integrity in rats with peripheral nerve injury, and this is achieved by changing the gene sequence at the injured point.</description><subject>Acupuncture</subject><subject>Analysis</subject><subject>Animals</subject><subject>Atrophy</subject><subject>Cell differentiation</subject><subject>Chinese medicine</subject><subject>Gene expression</subject><subject>Intervention</subject><subject>Investigations</subject><subject>Methods</subject><subject>Microscopy</subject><subject>Mitochondria</subject><subject>Myelin</subject><subject>Myoblasts</subject><subject>Myocytes</subject><subject>Myotubes</subject><subject>Neurodegeneration</subject><subject>Peripheral nerves</subject><subject>Recovery of function</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Sciatic nerve</subject><subject>Skeletal muscle</subject><subject>Skin</subject><subject>Transmission electron microscopy</subject><subject>Ultrastructure</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkcuP0zAQxiMEYh9w44wicUFaytqOnxekasWj0gISFImb5TqTxlVq7zpJ0f73TGjpAidOtmZ-_jzzfUXxjJLXlApxyQgjl0ILw4x-UJxSxemMM60fHu_q-0lx1vcbQphRSj0uTiqmGK-YPC3qeSwXcQf9ENZuCCmWIQ6pHFoov0DrVqELA9Z3UH4E37oY-m2ZmnI5hugQ_QUuM7hhC3GYOl99QN6XnyDjo0XcjPnuSfGocV0PTw_nefHt3dvl1YfZ9ef3i6v59cxzY4aZ1ER7XjOmgFbEeekq7sAIwYxcSW20N0RggfFaSs4JMCK0qoivBbaRPi_e7HVvxtUWao8jZdfZmxy2Lt_Z5IL9uxNDa9dpZ1Wl0ZEKBV4eBHK6HdEUuw29h65zEdLYW4bGcqqU1oi--AfdpDFHXG-iFKMGTb6n1q4DG2KT8F8_idq5rIzgmkuD1Ks95XPq-wzNcWRK7BSynUK2h5ARf_7nmkf4d6oIXOyBNsTa_Qj_KQfIQOPuaSqkkqT6CY7ptyM</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Wong, Steven</creator><creator>Lu, MengQian</creator><creator>Luo, Yuting</creator><creator>Shao, Shuai</creator><creator>Zhang, Yumo</creator><creator>Yu, TianYuan</creator><creator>Mo, Yanjun</creator><creator>Lv, TaoTao</creator><creator>Shen, Yi</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6838-3851</orcidid><orcidid>https://orcid.org/0000-0003-2089-3101</orcidid></search><sort><creationdate>2020</creationdate><title>An Investigation into the Rehabilitative Mechanism of Tuina in the Treatment of Sciatic Nerve Injury</title><author>Wong, Steven ; Lu, MengQian ; Luo, Yuting ; Shao, Shuai ; Zhang, Yumo ; Yu, TianYuan ; Mo, Yanjun ; Lv, TaoTao ; Shen, Yi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-6808c4d227e130ac6a34ae955296b6898c905ae924d66440e2058730cd5b686a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acupuncture</topic><topic>Analysis</topic><topic>Animals</topic><topic>Atrophy</topic><topic>Cell differentiation</topic><topic>Chinese medicine</topic><topic>Gene expression</topic><topic>Intervention</topic><topic>Investigations</topic><topic>Methods</topic><topic>Microscopy</topic><topic>Mitochondria</topic><topic>Myelin</topic><topic>Myoblasts</topic><topic>Myocytes</topic><topic>Myotubes</topic><topic>Neurodegeneration</topic><topic>Peripheral nerves</topic><topic>Recovery of function</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Sciatic nerve</topic><topic>Skeletal muscle</topic><topic>Skin</topic><topic>Transmission electron microscopy</topic><topic>Ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wong, Steven</creatorcontrib><creatorcontrib>Lu, MengQian</creatorcontrib><creatorcontrib>Luo, Yuting</creatorcontrib><creatorcontrib>Shao, Shuai</creatorcontrib><creatorcontrib>Zhang, Yumo</creatorcontrib><creatorcontrib>Yu, TianYuan</creatorcontrib><creatorcontrib>Mo, Yanjun</creatorcontrib><creatorcontrib>Lv, TaoTao</creatorcontrib><creatorcontrib>Shen, Yi</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Psychology Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wong, Steven</au><au>Lu, MengQian</au><au>Luo, Yuting</au><au>Shao, Shuai</au><au>Zhang, Yumo</au><au>Yu, TianYuan</au><au>Mo, Yanjun</au><au>Lv, TaoTao</au><au>Shen, Yi</au><au>Duann, Jeng-Ren</au><au>Jeng-Ren Duann</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Investigation into the Rehabilitative Mechanism of Tuina in the Treatment of Sciatic Nerve Injury</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2020</date><risdate>2020</risdate><volume>2020</volume><issue>2020</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Objective. To explore the effect of tuina on the gene expression at the point of nerve injury in rats with sciatic nerve injury (SNI) and to elucidate the repair mechanism of tuina promoting the functional recovery of peripheral nerve injury. Methods. In the Sham group, the right sciatic nerve was exposed without clamping. The SNI model was established using the sciatic nerve clamp method on the right leg and then randomly divided into the SNI group and the Tuina group. Seven days after modeling, the Tuina group was treated daily with a “massage and tuina manipulation simulator” (Patent No. ZL 2007 0187403.1), which was used daily to stimulate Yinmen (BL37), Yanglingquan (GB34), and Chengshan (BL57) with point-pressing method, plucking method, and kneading method. The stimulating force was 4N, and the stimulating frequency was 60 times per minute; each method and each point were used for 1 minute, totaling 9 minutes (1 min/acupoint/method × 3 methods × 3 acupoints). Treatment was administered for 21 days, followed by a 1-day rest after the 10th treatment, for a total of 20 times of intervention. The sciatic function index (SFI) was used to evaluate the fine movements of the hind limbs of rats in each group. The ultrastructural changes at the point of nerve injury were observed by transmission electron microscopy, and the gene changes at the point of nerve injury were detected using RNA-sequencing (RNA-seq) technology. Results. Compared with the baseline, the SFI of the SNI group and the Tuina group decreased significantly at the 0th intervention (7 days after molding); compared with the SNI group, the SFI of the Tuina group increased at the 10th intervention (P<0.05) and increased significantly at the 15th and 20th intervention (P<0.01). Compared with the Sham group, the myelin sheath integrity of the sciatic nerve in the SNI group was destroyed and the myelin sheath collapsed seriously, even forming myelin sheath ball, accompanied with severe axonal atrophy and mitochondrial degeneration. The tuina intervention could significantly improve the ultrastructure of the nerve injury point, and the nerve fiber myelin sheath in the Tuina group remained intact, without obvious axonal swelling or atrophy. Atrophic thread granules could be seen in the axon, but there were no vacuolated mitochondria. RNA-seq results showed that there were differences at 221 genes at the point of nerve injury between the Tuina group and the SNI group and the differentially expressed genes (DEGs) are enriched in the biological processes related to the regulation of myocyte. Regulations include the regulation of striated muscle cell differentiation, myoblast differentiation, and myotube differentiation. Conclusion. Tuina can improve the fine motor recovery and protect the myelin integrity in rats with peripheral nerve injury, and this is achieved by changing the gene sequence at the injured point.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>32724326</pmid><doi>10.1155/2020/5859298</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-6838-3851</orcidid><orcidid>https://orcid.org/0000-0003-2089-3101</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1741-427X |
| ispartof | Evidence-based complementary and alternative medicine, 2020, Vol.2020 (2020), p.1-11 |
| issn | 1741-427X 1741-4288 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7382723 |
| source | PubMed Central Open Access; Wiley-Blackwell Open Access Titles; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Acupuncture Analysis Animals Atrophy Cell differentiation Chinese medicine Gene expression Intervention Investigations Methods Microscopy Mitochondria Myelin Myoblasts Myocytes Myotubes Neurodegeneration Peripheral nerves Recovery of function Ribonucleic acid RNA Sciatic nerve Skeletal muscle Skin Transmission electron microscopy Ultrastructure |
| title | An Investigation into the Rehabilitative Mechanism of Tuina in the Treatment of Sciatic Nerve Injury |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T15%3A24%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20Investigation%20into%20the%20Rehabilitative%20Mechanism%20of%20Tuina%20in%20the%20Treatment%20of%20Sciatic%20Nerve%20Injury&rft.jtitle=Evidence-based%20complementary%20and%20alternative%20medicine&rft.au=Wong,%20Steven&rft.date=2020&rft.volume=2020&rft.issue=2020&rft.spage=1&rft.epage=11&rft.pages=1-11&rft.issn=1741-427X&rft.eissn=1741-4288&rft_id=info:doi/10.1155/2020/5859298&rft_dat=%3Cgale_pubme%3EA639548469%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2427219327&rft_id=info:pmid/32724326&rft_galeid=A639548469&rfr_iscdi=true |