Suppressing HOXA-10 Gene Expression by MicroRNA 135b During the Window of Implantation in Infertile Women
Implantation failure has long been identified as a common problem underlying low success rate of IVF. Currently, endometrial receptivity has gained expert attention as it is demonstrated to contribute to successful embryo implantation. MicroRNAs (miRNAs) is known to affect endometrial receptivity th...
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| Veröffentlicht in: | Journal of reproduction & infertility 2020-07, Vol.21 (3), p.217-221 |
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| container_title | Journal of reproduction & infertility |
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| creator | Riyanti, Aida Febri, Ririn Rahmala Zakirah, Sarah Chairani Harzif, Achmad Kemal Rajuddin, Rajuddin Muharam, Raden Asmarinah, Asmarinah Wiweko, Budi |
| description | Implantation failure has long been identified as a common problem underlying low success rate of IVF. Currently, endometrial receptivity has gained expert attention as it is demonstrated to contribute to successful embryo implantation. MicroRNAs (miRNAs) is known to affect endometrial receptivity through post-transcriptional gene expression regulation. This study aimed to evaluate the expression of miRNA 135b and HOXA-10 during the implantation window in endometrial tissue of infertile women.
A total of 14 patients diagnosed with infertility in the gynaecology clinic of Cipto Mangunkusumo and Daya Medika hospitals Jakart, Indonesia were selected as the observed group, and 9 fertile patients were enrolled in the control group. Total RNA was isolated from endometrial tissues collected at the secretory phase of the menstrual cycle. The miRNA 135b and HOXA-10 mRNA expression were measured using quantitative real-time PCR (qPCR). The correlation between these variables was then determined using Pearson's correlation coefficient.
The expression of miRNA 135b in the infertile group was significantly higher by 1.81-fold compared to the control group (p |
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A total of 14 patients diagnosed with infertility in the gynaecology clinic of Cipto Mangunkusumo and Daya Medika hospitals Jakart, Indonesia were selected as the observed group, and 9 fertile patients were enrolled in the control group. Total RNA was isolated from endometrial tissues collected at the secretory phase of the menstrual cycle. The miRNA 135b and HOXA-10 mRNA expression were measured using quantitative real-time PCR (qPCR). The correlation between these variables was then determined using Pearson's correlation coefficient.
The expression of miRNA 135b in the infertile group was significantly higher by 1.81-fold compared to the control group (p<0.01), whereas, expression of HOXA-10 mRNA was significantly lower in the infertile group compared to the controls (p=0.047). Significant negative correlation was observed between the expression of miRNA 135b and HOXA-10 mRNA in infertile women (p=0.021; r=-0.607).
Taken together, this study provides that alteration of miRNA expression is involved in regulating the implantation process partly via modulation of the expression of gene required for implantation.</description><identifier>ISSN: 2228-5482</identifier><identifier>EISSN: 2251-676X</identifier><identifier>PMID: 32685419</identifier><language>eng</language><publisher>Iran: Office for Scientific Journals</publisher><subject>Correlation coefficient ; Correlation coefficients ; Embryos ; Endometrium ; Gene expression ; Gene regulation ; Implantation ; Infertility ; Menstrual cycle ; MicroRNAs ; miRNA ; Patients ; Post-transcription ; Ribonucleic acid ; RNA ; Short Communication</subject><ispartof>Journal of reproduction & infertility, 2020-07, Vol.21 (3), p.217-221</ispartof><rights>Copyright© 2020, Avicenna Research Institute.</rights><rights>Copyright Office for Scientific Journals 2020</rights><rights>Copyright© 2020, Avicenna Research Institute. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7362087/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7362087/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32685419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Riyanti, Aida</creatorcontrib><creatorcontrib>Febri, Ririn Rahmala</creatorcontrib><creatorcontrib>Zakirah, Sarah Chairani</creatorcontrib><creatorcontrib>Harzif, Achmad Kemal</creatorcontrib><creatorcontrib>Rajuddin, Rajuddin</creatorcontrib><creatorcontrib>Muharam, Raden</creatorcontrib><creatorcontrib>Asmarinah, Asmarinah</creatorcontrib><creatorcontrib>Wiweko, Budi</creatorcontrib><title>Suppressing HOXA-10 Gene Expression by MicroRNA 135b During the Window of Implantation in Infertile Women</title><title>Journal of reproduction & infertility</title><addtitle>J Reprod Infertil</addtitle><description>Implantation failure has long been identified as a common problem underlying low success rate of IVF. Currently, endometrial receptivity has gained expert attention as it is demonstrated to contribute to successful embryo implantation. MicroRNAs (miRNAs) is known to affect endometrial receptivity through post-transcriptional gene expression regulation. This study aimed to evaluate the expression of miRNA 135b and HOXA-10 during the implantation window in endometrial tissue of infertile women.
A total of 14 patients diagnosed with infertility in the gynaecology clinic of Cipto Mangunkusumo and Daya Medika hospitals Jakart, Indonesia were selected as the observed group, and 9 fertile patients were enrolled in the control group. Total RNA was isolated from endometrial tissues collected at the secretory phase of the menstrual cycle. The miRNA 135b and HOXA-10 mRNA expression were measured using quantitative real-time PCR (qPCR). The correlation between these variables was then determined using Pearson's correlation coefficient.
The expression of miRNA 135b in the infertile group was significantly higher by 1.81-fold compared to the control group (p<0.01), whereas, expression of HOXA-10 mRNA was significantly lower in the infertile group compared to the controls (p=0.047). Significant negative correlation was observed between the expression of miRNA 135b and HOXA-10 mRNA in infertile women (p=0.021; r=-0.607).
Taken together, this study provides that alteration of miRNA expression is involved in regulating the implantation process partly via modulation of the expression of gene required for implantation.</description><subject>Correlation coefficient</subject><subject>Correlation coefficients</subject><subject>Embryos</subject><subject>Endometrium</subject><subject>Gene expression</subject><subject>Gene regulation</subject><subject>Implantation</subject><subject>Infertility</subject><subject>Menstrual cycle</subject><subject>MicroRNAs</subject><subject>miRNA</subject><subject>Patients</subject><subject>Post-transcription</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Short Communication</subject><issn>2228-5482</issn><issn>2251-676X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdUU1Lw0AQDaLYUvsXZMGLl0D2K9m9CKXWtlAt-IG9hU2ybbcku3E3Ufvv3dAq6lxmmHnz5j3mJOgjRGEYJ_HqtKsRCylhqBcMndtFPjiHCPPzoIdRzCiBvB-op7aurXRO6Q2YLVejEEZgKrUEk89D32iQ7cG9yq15fBgBiGkGblvb4ZutBK9KF-YDmDWYV3UpdCOabkVpMNdraRtVeoyppL4IztaidHJ4zIPg5W7yPJ6Fi-V0Ph4twhpFvAnlWpKoICgXIsE4RlSwOKE0EYIxSSijnGbYS895RAQrOGW4IJz5tYTEkFA8CG4OvHWbVbLIpW6sKNPaqkrYfWqESv9OtNqmG_OeJv5axBJPcH0ksOatla5JK-VyWXpz0rQuRQRRygmKsYde_YPuTGu1t5eiGEaYM8g6RZe_Ff1I-f4C_gJanoPY</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Riyanti, Aida</creator><creator>Febri, Ririn Rahmala</creator><creator>Zakirah, Sarah Chairani</creator><creator>Harzif, Achmad Kemal</creator><creator>Rajuddin, Rajuddin</creator><creator>Muharam, Raden</creator><creator>Asmarinah, Asmarinah</creator><creator>Wiweko, Budi</creator><general>Office for Scientific Journals</general><general>Avicenna Research Institute</general><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T5</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P64</scope><scope>PADUT</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200701</creationdate><title>Suppressing HOXA-10 Gene Expression by MicroRNA 135b During the Window of Implantation in Infertile Women</title><author>Riyanti, Aida ; Febri, Ririn Rahmala ; Zakirah, Sarah Chairani ; Harzif, Achmad Kemal ; Rajuddin, Rajuddin ; Muharam, Raden ; Asmarinah, Asmarinah ; Wiweko, Budi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p209t-efe40d42caa733625a867557aa88e458595b3419c904a8d9583d498efe7461453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Correlation coefficient</topic><topic>Correlation coefficients</topic><topic>Embryos</topic><topic>Endometrium</topic><topic>Gene expression</topic><topic>Gene regulation</topic><topic>Implantation</topic><topic>Infertility</topic><topic>Menstrual cycle</topic><topic>MicroRNAs</topic><topic>miRNA</topic><topic>Patients</topic><topic>Post-transcription</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Short Communication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Riyanti, Aida</creatorcontrib><creatorcontrib>Febri, Ririn Rahmala</creatorcontrib><creatorcontrib>Zakirah, Sarah Chairani</creatorcontrib><creatorcontrib>Harzif, Achmad Kemal</creatorcontrib><creatorcontrib>Rajuddin, Rajuddin</creatorcontrib><creatorcontrib>Muharam, Raden</creatorcontrib><creatorcontrib>Asmarinah, Asmarinah</creatorcontrib><creatorcontrib>Wiweko, Budi</creatorcontrib><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Research Library China</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of reproduction & infertility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Riyanti, Aida</au><au>Febri, Ririn Rahmala</au><au>Zakirah, Sarah Chairani</au><au>Harzif, Achmad Kemal</au><au>Rajuddin, Rajuddin</au><au>Muharam, Raden</au><au>Asmarinah, Asmarinah</au><au>Wiweko, Budi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suppressing HOXA-10 Gene Expression by MicroRNA 135b During the Window of Implantation in Infertile Women</atitle><jtitle>Journal of reproduction & infertility</jtitle><addtitle>J Reprod Infertil</addtitle><date>2020-07-01</date><risdate>2020</risdate><volume>21</volume><issue>3</issue><spage>217</spage><epage>221</epage><pages>217-221</pages><issn>2228-5482</issn><eissn>2251-676X</eissn><abstract>Implantation failure has long been identified as a common problem underlying low success rate of IVF. Currently, endometrial receptivity has gained expert attention as it is demonstrated to contribute to successful embryo implantation. MicroRNAs (miRNAs) is known to affect endometrial receptivity through post-transcriptional gene expression regulation. This study aimed to evaluate the expression of miRNA 135b and HOXA-10 during the implantation window in endometrial tissue of infertile women.
A total of 14 patients diagnosed with infertility in the gynaecology clinic of Cipto Mangunkusumo and Daya Medika hospitals Jakart, Indonesia were selected as the observed group, and 9 fertile patients were enrolled in the control group. Total RNA was isolated from endometrial tissues collected at the secretory phase of the menstrual cycle. The miRNA 135b and HOXA-10 mRNA expression were measured using quantitative real-time PCR (qPCR). The correlation between these variables was then determined using Pearson's correlation coefficient.
The expression of miRNA 135b in the infertile group was significantly higher by 1.81-fold compared to the control group (p<0.01), whereas, expression of HOXA-10 mRNA was significantly lower in the infertile group compared to the controls (p=0.047). Significant negative correlation was observed between the expression of miRNA 135b and HOXA-10 mRNA in infertile women (p=0.021; r=-0.607).
Taken together, this study provides that alteration of miRNA expression is involved in regulating the implantation process partly via modulation of the expression of gene required for implantation.</abstract><cop>Iran</cop><pub>Office for Scientific Journals</pub><pmid>32685419</pmid><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of reproduction & infertility, 2020-07, Vol.21 (3), p.217-221 |
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| subjects | Correlation coefficient Correlation coefficients Embryos Endometrium Gene expression Gene regulation Implantation Infertility Menstrual cycle MicroRNAs miRNA Patients Post-transcription Ribonucleic acid RNA Short Communication |
| title | Suppressing HOXA-10 Gene Expression by MicroRNA 135b During the Window of Implantation in Infertile Women |
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