Biotechnological Strategies for Chitosan Production by Mucoralean Strains and Dimorphism Using Renewable Substrates
We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by UCP 1262 and UCP 1266. The highest biomass yields of 4.832 g/L ( UCP 1262) and 6.345 g/L ( UCP 1266) were produced in assa...
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| Veröffentlicht in: | International journal of molecular sciences 2020-06, Vol.21 (12), p.4286 |
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| creator | de Souza, Adriana Ferreira Galindo, Hugo Marques de Lima, Marcos Antônio Barbosa Ribeaux, Daylin Rubio Rodríguez, Dayana Montero da Silva Andrade, Rosileide Fontenele Gusmão, Norma Buarque de Campos-Takaki, Galba Maria |
| description | We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by
UCP 1262 and
UCP 1266. The highest biomass yields of 4.832 g/L (
UCP 1262) and 6.345 g/L (
UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 2
, and also favored higher chitosan production (32.471 mg/g) for
. The highest chitosan production (44.91 mg/g) by
(UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL). The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains. The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by
(UCP 1262) and
(UCP 1266), respectively.
(UCP 1262) showed dimorphism in assay 4-6% CSL and 8% CWW and central point.
(UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW. The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production. |
| doi_str_mv | 10.3390/ijms21124286 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7352823</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2415175476</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-d781c0f0afda06f142f8631a57ab0de24db9639d91e6cce342b76080d69251643</originalsourceid><addsrcrecordid>eNpVUctOwzAQtBCIlsKNM7LElYAfiZNckKA8pSIQpWfLsZ3WVWIXOwH170lpqcppVtrZmVkNAKcYXVKaoyszrwPBmMQkY3ugj2NCIoRYur8z98BRCHOECCVJfgh6HTBEMO2DcGtco-XMuspNjRQVHDdeNHpqdICl83A4M40LwsI371QrG-MsLJbwpZXOi0p3i9WBsQEKq-CdqZ1fzEyo4SQYO4Xv2upvUVQajtsi_EqHY3BQiirokw0OwOTh_mP4FI1eH5-HN6NIxpg0kUozLFGJRKkEYmX3TJkxikWSigIpTWJV5IzmKseaSalpTIqUoQwplpMEs5gOwPVad9EWtVZS286_4gtvauGX3AnD_2-smfGp--IpTUhGaCdwvhHw7rPVoeFz13rbZeYkxglOkzhlHetizZLeheB1uXXAiK8q4rsVdfSz3VRb8l8n9AdUso-t</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2415175476</pqid></control><display><type>article</type><title>Biotechnological Strategies for Chitosan Production by Mucoralean Strains and Dimorphism Using Renewable Substrates</title><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>de Souza, Adriana Ferreira ; Galindo, Hugo Marques ; de Lima, Marcos Antônio Barbosa ; Ribeaux, Daylin Rubio ; Rodríguez, Dayana Montero ; da Silva Andrade, Rosileide Fontenele ; Gusmão, Norma Buarque ; de Campos-Takaki, Galba Maria</creator><creatorcontrib>de Souza, Adriana Ferreira ; Galindo, Hugo Marques ; de Lima, Marcos Antônio Barbosa ; Ribeaux, Daylin Rubio ; Rodríguez, Dayana Montero ; da Silva Andrade, Rosileide Fontenele ; Gusmão, Norma Buarque ; de Campos-Takaki, Galba Maria</creatorcontrib><description>We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by
UCP 1262 and
UCP 1266. The highest biomass yields of 4.832 g/L (
UCP 1262) and 6.345 g/L (
UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 2
, and also favored higher chitosan production (32.471 mg/g) for
. The highest chitosan production (44.91 mg/g) by
(UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL). The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains. The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by
(UCP 1262) and
(UCP 1266), respectively.
(UCP 1262) showed dimorphism in assay 4-6% CSL and 8% CWW and central point.
(UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW. The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms21124286</identifier><identifier>PMID: 32560213</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Acetylation ; Antimicrobial agents ; Biocompatibility ; Biomass ; Carbon ; Carbon - metabolism ; Carbon sources ; Cassava ; Chitosan ; Chitosan - metabolism ; Crustaceans ; Deacetylation ; Dimorphism ; Factorial design ; Fungi ; Industrial wastes ; Liquor ; Manihot - chemistry ; Morphology ; Mucor - growth & development ; Mucor - metabolism ; Mucorales - growth & development ; Mucorales - metabolism ; Nitrogen ; Nitrogen - metabolism ; Nitrogen sources ; Spectroscopy, Fourier Transform Infrared ; Statistical analysis ; Waste Water - chemistry ; Wastewater ; Zea mays - chemistry</subject><ispartof>International journal of molecular sciences, 2020-06, Vol.21 (12), p.4286</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-d781c0f0afda06f142f8631a57ab0de24db9639d91e6cce342b76080d69251643</citedby><cites>FETCH-LOGICAL-c412t-d781c0f0afda06f142f8631a57ab0de24db9639d91e6cce342b76080d69251643</cites><orcidid>0000-0001-8954-7309 ; 0000-0002-0519-0849 ; 0000-0002-5872-5659</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352823/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352823/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32560213$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Souza, Adriana Ferreira</creatorcontrib><creatorcontrib>Galindo, Hugo Marques</creatorcontrib><creatorcontrib>de Lima, Marcos Antônio Barbosa</creatorcontrib><creatorcontrib>Ribeaux, Daylin Rubio</creatorcontrib><creatorcontrib>Rodríguez, Dayana Montero</creatorcontrib><creatorcontrib>da Silva Andrade, Rosileide Fontenele</creatorcontrib><creatorcontrib>Gusmão, Norma Buarque</creatorcontrib><creatorcontrib>de Campos-Takaki, Galba Maria</creatorcontrib><title>Biotechnological Strategies for Chitosan Production by Mucoralean Strains and Dimorphism Using Renewable Substrates</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by
UCP 1262 and
UCP 1266. The highest biomass yields of 4.832 g/L (
UCP 1262) and 6.345 g/L (
UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 2
, and also favored higher chitosan production (32.471 mg/g) for
. The highest chitosan production (44.91 mg/g) by
(UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL). The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains. The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by
(UCP 1262) and
(UCP 1266), respectively.
(UCP 1262) showed dimorphism in assay 4-6% CSL and 8% CWW and central point.
(UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW. The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production.</description><subject>Acetylation</subject><subject>Antimicrobial agents</subject><subject>Biocompatibility</subject><subject>Biomass</subject><subject>Carbon</subject><subject>Carbon - metabolism</subject><subject>Carbon sources</subject><subject>Cassava</subject><subject>Chitosan</subject><subject>Chitosan - metabolism</subject><subject>Crustaceans</subject><subject>Deacetylation</subject><subject>Dimorphism</subject><subject>Factorial design</subject><subject>Fungi</subject><subject>Industrial wastes</subject><subject>Liquor</subject><subject>Manihot - chemistry</subject><subject>Morphology</subject><subject>Mucor - growth & development</subject><subject>Mucor - metabolism</subject><subject>Mucorales - growth & development</subject><subject>Mucorales - metabolism</subject><subject>Nitrogen</subject><subject>Nitrogen - metabolism</subject><subject>Nitrogen sources</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Statistical analysis</subject><subject>Waste Water - 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UCP 1262 and
UCP 1266. The highest biomass yields of 4.832 g/L (
UCP 1262) and 6.345 g/L (
UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 2
, and also favored higher chitosan production (32.471 mg/g) for
. The highest chitosan production (44.91 mg/g) by
(UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL). The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains. The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by
(UCP 1262) and
(UCP 1266), respectively.
(UCP 1262) showed dimorphism in assay 4-6% CSL and 8% CWW and central point.
(UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW. The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>32560213</pmid><doi>10.3390/ijms21124286</doi><orcidid>https://orcid.org/0000-0001-8954-7309</orcidid><orcidid>https://orcid.org/0000-0002-0519-0849</orcidid><orcidid>https://orcid.org/0000-0002-5872-5659</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of molecular sciences, 2020-06, Vol.21 (12), p.4286 |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Acetylation Antimicrobial agents Biocompatibility Biomass Carbon Carbon - metabolism Carbon sources Cassava Chitosan Chitosan - metabolism Crustaceans Deacetylation Dimorphism Factorial design Fungi Industrial wastes Liquor Manihot - chemistry Morphology Mucor - growth & development Mucor - metabolism Mucorales - growth & development Mucorales - metabolism Nitrogen Nitrogen - metabolism Nitrogen sources Spectroscopy, Fourier Transform Infrared Statistical analysis Waste Water - chemistry Wastewater Zea mays - chemistry |
| title | Biotechnological Strategies for Chitosan Production by Mucoralean Strains and Dimorphism Using Renewable Substrates |
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