Determining calcium concentration in heterogeneous model systems using multiple indicators

Abstract Intracellular free calcium concentrations ([Ca2+ ] i ) are assessed by measuring indicator fluorescence in entire cells or subcellular regions using fluorescence microscopy. [Ca2+ ] i is calculated using equations which link fluorescence intensities (or intensity ratios) to calcium concentr...

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Veröffentlicht in:	Cell calcium (Edinburgh) 2007-12, Vol.42 (6), p.576-589
Hauptverfasser:	Hyrc, Krzysztof L, Rzeszotnik, Ziemowit, Kennedy, Bryan R, Goldberg, Mark P
Format:	Artikel
Sprache:	eng
Schlagworte:	Advanced Basic Science Algorithms Calcium - analysis Calcium - metabolism Calcium domains Fluorescence Fluorescent indicators Indicator affinity Intracellular Fluid - metabolism Intracellular free calcium concentration measurements Microscope resolution Microscopy, Fluorescence Models, Theoretical
Online-Zugang:	Volltext
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