Mapping domains of ARS2 critical for its RNA decay capacity

Abstract ARS2 is a conserved protein centrally involved in both nuclear RNA productive and destructive processes. To map features of ARS2 promoting RNA decay, we utilized two different RNA reporters, one of which depends on direct ARS2 tethering for its degradation. In both cases, ARS2 triggers a de...

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Veröffentlicht in:Nucleic acids research 2020-07, Vol.48 (12), p.6943-6953
Hauptverfasser: Melko, Mireille, Winczura, Kinga, Rouvière, Jérôme Olivier, Oborská-Oplová, Michaela, Andersen, Pia K, Heick Jensen, Torben
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container_end_page 6953
container_issue 12
container_start_page 6943
container_title Nucleic acids research
container_volume 48
creator Melko, Mireille
Winczura, Kinga
Rouvière, Jérôme Olivier
Oborská-Oplová, Michaela
Andersen, Pia K
Heick Jensen, Torben
description Abstract ARS2 is a conserved protein centrally involved in both nuclear RNA productive and destructive processes. To map features of ARS2 promoting RNA decay, we utilized two different RNA reporters, one of which depends on direct ARS2 tethering for its degradation. In both cases, ARS2 triggers a degradation phenotype aided by its interaction with the poly(A) tail exosome targeting (PAXT) connection. Interestingly, C-terminal amino acids of ARS2, responsible for binding the RNA 5′cap binding complex (CBC), become dispensable when ARS2 is directly tethered to the reporter RNA. In contrast, the Zinc-finger (ZnF) domain of ARS2 is essential for the decay of both reporters and consistently co-immunoprecipitation analyses reveal a necessity of this domain for the interaction of ARS2 with the PAXT-associated RNA helicase MTR4. Taken together, our results map the domains of ARS2 underlying two essential properties of the protein: its RNP targeting ability and its capacity to recruit the RNA decay machinery.
doi_str_mv 10.1093/nar/gkaa445
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subjects Exosome Multienzyme Ribonuclease Complex - genetics
HEK293 Cells
Humans
Nuclear Cap-Binding Protein Complex - genetics
Nuclear Proteins - chemistry
Nuclear Proteins - genetics
Protein Domains - genetics
RNA and RNA-protein complexes
RNA Helicases - chemistry
RNA Helicases - genetics
RNA Stability - genetics
RNA, Messenger - chemistry
RNA, Messenger - genetics
RNA, Nuclear - chemistry
RNA, Nuclear - genetics
title Mapping domains of ARS2 critical for its RNA decay capacity
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