Prevalence of Bartonella spp. Infection in Patients with Sickle Cell Disease
Background: The inherent characteristics of the sickle cell disease (SCD), the most common genetic hematological disorder, increase the propensity of infections. Bartonella spp. are emerging and neglected bacteria. A large spectrum of clinical manifestations has been linked to bartonella bloodstream...
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| Veröffentlicht in: | Vector borne and zoonotic diseases (Larchmont, N.Y.) N.Y.), 2020-07, Vol.20 (7), p.59-512 |
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| creator | Soares, Tânia Cristina Benetti Isaias, Gustavo Alves Brito Almeida, Amanda Roberta de Drummond, Marina Rovani da Silva, Marilene Neves Lania, Bruno Grosselli Vieira-Damiani, Gislaine Saad, Sara Teresinha Olalla Ericson, Marna Elise Gupta, Kalpna Velho, Paulo Eduardo Neves Ferreira |
| description | Background:
The inherent characteristics of the sickle cell disease (SCD), the most common genetic hematological disorder, increase the propensity of infections.
Bartonella
spp. are emerging and neglected bacteria. A large spectrum of clinical manifestations has been linked to bartonella bloodstream infection in the last two decades that can cause fatal outcomes, especially in immunodeficient patients. The goal of this study was to evaluate the prevalence of bartonella infection in SCD patients.
Materials and Methods:
We evaluated
Bartonella
spp. prevalence in 107 SCD patients. Blood samples and enrichment blood cultures were analyzed by molecular detection of
Bartonella
spp. DNA. Bartonella DNA was amplified using conventional genus-specific
Bartonella
PCR which amplifies the Intergenic Transcribed Spacer region and
Bartonella henselae-
specific nested PCR which amplifies the
FtsZ
gene. Positive patient DNAs were tested with ssrA conventional PCR. All amplicons were sequenced.
Findings:
Ten of 107 patients tested positive for
B. henselae
infection in at least one molecular test. All obtained amplicons were sequenced and similar to
B. henselae
sequences deposited in GenBank (accession number BX897699). Based on statistical results, bloodstream infection with
B. henselae
was not associated with animal contact or blood transfusions.
Conclusion:
We detected
B. henselae
DNA in 10 (9.3%) SCD studied patients. These patients were notified and treatment was offered to them. |
| doi_str_mv | 10.1089/vbz.2019.2545 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7336878</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2350906011</sourcerecordid><originalsourceid>FETCH-LOGICAL-c459t-8605b4cfb77888f7cb47089db5810605620244c7fb50fcc37508c64b141069483</originalsourceid><addsrcrecordid>eNqFkc1LBCEAxSWKvo9dQ-jSZTYddXQuQW2fsFBQnUVdp6xZ3XR2o_76HHaL6tJJ8f18vMcDYA-jAUaiPprrj0GJcD0oGWUrYBMzxgvOWb3a3wkqSFXxDbCV0jNCJRaYrYMNkn8QzsUmGN1GO1et9cbC0MBTFbvgbdsqmKbTAbz2jTWdCx46D29V56zvEnxz3RO8c-altXCYYXjmklXJ7oC1RrXJ7i7PbfBwcX4_vCpGN5fXw5NRYSiru0JUiGlqGp0TCNFwoynPVcaaCYyyVpWopNTwRjPUGEM4Q8JUVGOa5ZoKsg2OF77TmZ7YscmhomrlNLqJiu8yKCd_K949yccwl5yQSvDe4HBpEMPrzKZOTlwyfW1vwyzJkjBU5ygYZ_TgD_ocZtHnerKkJapyWoIyVSwoE0NK0TbfYTCS_U4y7yT7nWS_U-b3fzb4pr-GyQBZAP2z8r51VtvY_WP7CeQfniU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2420656230</pqid></control><display><type>article</type><title>Prevalence of Bartonella spp. Infection in Patients with Sickle Cell Disease</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Soares, Tânia Cristina Benetti ; Isaias, Gustavo Alves Brito ; Almeida, Amanda Roberta de ; Drummond, Marina Rovani ; da Silva, Marilene Neves ; Lania, Bruno Grosselli ; Vieira-Damiani, Gislaine ; Saad, Sara Teresinha Olalla ; Ericson, Marna Elise ; Gupta, Kalpna ; Velho, Paulo Eduardo Neves Ferreira</creator><creatorcontrib>Soares, Tânia Cristina Benetti ; Isaias, Gustavo Alves Brito ; Almeida, Amanda Roberta de ; Drummond, Marina Rovani ; da Silva, Marilene Neves ; Lania, Bruno Grosselli ; Vieira-Damiani, Gislaine ; Saad, Sara Teresinha Olalla ; Ericson, Marna Elise ; Gupta, Kalpna ; Velho, Paulo Eduardo Neves Ferreira</creatorcontrib><description>Background:
The inherent characteristics of the sickle cell disease (SCD), the most common genetic hematological disorder, increase the propensity of infections.
Bartonella
spp. are emerging and neglected bacteria. A large spectrum of clinical manifestations has been linked to bartonella bloodstream infection in the last two decades that can cause fatal outcomes, especially in immunodeficient patients. The goal of this study was to evaluate the prevalence of bartonella infection in SCD patients.
Materials and Methods:
We evaluated
Bartonella
spp. prevalence in 107 SCD patients. Blood samples and enrichment blood cultures were analyzed by molecular detection of
Bartonella
spp. DNA. Bartonella DNA was amplified using conventional genus-specific
Bartonella
PCR which amplifies the Intergenic Transcribed Spacer region and
Bartonella henselae-
specific nested PCR which amplifies the
FtsZ
gene. Positive patient DNAs were tested with ssrA conventional PCR. All amplicons were sequenced.
Findings:
Ten of 107 patients tested positive for
B. henselae
infection in at least one molecular test. All obtained amplicons were sequenced and similar to
B. henselae
sequences deposited in GenBank (accession number BX897699). Based on statistical results, bloodstream infection with
B. henselae
was not associated with animal contact or blood transfusions.
Conclusion:
We detected
B. henselae
DNA in 10 (9.3%) SCD studied patients. These patients were notified and treatment was offered to them.</description><identifier>ISSN: 1530-3667</identifier><identifier>EISSN: 1557-7759</identifier><identifier>DOI: 10.1089/vbz.2019.2545</identifier><identifier>PMID: 32013778</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Amplification ; Anemia, Sickle Cell - complications ; Bartonella ; Bartonella - isolation & purification ; Bartonella Infections - complications ; Bartonella Infections - epidemiology ; Blood transfusion ; Deoxyribonucleic acid ; DNA ; FtsZ gene ; Hematology ; Humans ; Immunodeficiency ; Infections ; Original ; Original Articles ; Patients ; Polymerase Chain Reaction ; Prevalence ; Risk Factors ; Sequences ; Sickle cell disease ; Spacer region ; Statistical methods</subject><ispartof>Vector borne and zoonotic diseases (Larchmont, N.Y.), 2020-07, Vol.20 (7), p.59-512</ispartof><rights>2020, Mary Ann Liebert, Inc., publishers</rights><rights>Copyright Mary Ann Liebert, Inc. Jul 2020</rights><rights>Copyright 2020, Mary Ann Liebert, Inc., publishers 2020 Mary Ann Liebert, Inc., publishers</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-8605b4cfb77888f7cb47089db5810605620244c7fb50fcc37508c64b141069483</citedby><cites>FETCH-LOGICAL-c459t-8605b4cfb77888f7cb47089db5810605620244c7fb50fcc37508c64b141069483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32013778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soares, Tânia Cristina Benetti</creatorcontrib><creatorcontrib>Isaias, Gustavo Alves Brito</creatorcontrib><creatorcontrib>Almeida, Amanda Roberta de</creatorcontrib><creatorcontrib>Drummond, Marina Rovani</creatorcontrib><creatorcontrib>da Silva, Marilene Neves</creatorcontrib><creatorcontrib>Lania, Bruno Grosselli</creatorcontrib><creatorcontrib>Vieira-Damiani, Gislaine</creatorcontrib><creatorcontrib>Saad, Sara Teresinha Olalla</creatorcontrib><creatorcontrib>Ericson, Marna Elise</creatorcontrib><creatorcontrib>Gupta, Kalpna</creatorcontrib><creatorcontrib>Velho, Paulo Eduardo Neves Ferreira</creatorcontrib><title>Prevalence of Bartonella spp. Infection in Patients with Sickle Cell Disease</title><title>Vector borne and zoonotic diseases (Larchmont, N.Y.)</title><addtitle>Vector Borne Zoonotic Dis</addtitle><description>Background:
The inherent characteristics of the sickle cell disease (SCD), the most common genetic hematological disorder, increase the propensity of infections.
Bartonella
spp. are emerging and neglected bacteria. A large spectrum of clinical manifestations has been linked to bartonella bloodstream infection in the last two decades that can cause fatal outcomes, especially in immunodeficient patients. The goal of this study was to evaluate the prevalence of bartonella infection in SCD patients.
Materials and Methods:
We evaluated
Bartonella
spp. prevalence in 107 SCD patients. Blood samples and enrichment blood cultures were analyzed by molecular detection of
Bartonella
spp. DNA. Bartonella DNA was amplified using conventional genus-specific
Bartonella
PCR which amplifies the Intergenic Transcribed Spacer region and
Bartonella henselae-
specific nested PCR which amplifies the
FtsZ
gene. Positive patient DNAs were tested with ssrA conventional PCR. All amplicons were sequenced.
Findings:
Ten of 107 patients tested positive for
B. henselae
infection in at least one molecular test. All obtained amplicons were sequenced and similar to
B. henselae
sequences deposited in GenBank (accession number BX897699). Based on statistical results, bloodstream infection with
B. henselae
was not associated with animal contact or blood transfusions.
Conclusion:
We detected
B. henselae
DNA in 10 (9.3%) SCD studied patients. These patients were notified and treatment was offered to them.</description><subject>Amplification</subject><subject>Anemia, Sickle Cell - complications</subject><subject>Bartonella</subject><subject>Bartonella - isolation & purification</subject><subject>Bartonella Infections - complications</subject><subject>Bartonella Infections - epidemiology</subject><subject>Blood transfusion</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>FtsZ gene</subject><subject>Hematology</subject><subject>Humans</subject><subject>Immunodeficiency</subject><subject>Infections</subject><subject>Original</subject><subject>Original Articles</subject><subject>Patients</subject><subject>Polymerase Chain Reaction</subject><subject>Prevalence</subject><subject>Risk Factors</subject><subject>Sequences</subject><subject>Sickle cell disease</subject><subject>Spacer region</subject><subject>Statistical methods</subject><issn>1530-3667</issn><issn>1557-7759</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1LBCEAxSWKvo9dQ-jSZTYddXQuQW2fsFBQnUVdp6xZ3XR2o_76HHaL6tJJ8f18vMcDYA-jAUaiPprrj0GJcD0oGWUrYBMzxgvOWb3a3wkqSFXxDbCV0jNCJRaYrYMNkn8QzsUmGN1GO1et9cbC0MBTFbvgbdsqmKbTAbz2jTWdCx46D29V56zvEnxz3RO8c-altXCYYXjmklXJ7oC1RrXJ7i7PbfBwcX4_vCpGN5fXw5NRYSiru0JUiGlqGp0TCNFwoynPVcaaCYyyVpWopNTwRjPUGEM4Q8JUVGOa5ZoKsg2OF77TmZ7YscmhomrlNLqJiu8yKCd_K949yccwl5yQSvDe4HBpEMPrzKZOTlwyfW1vwyzJkjBU5ygYZ_TgD_ocZtHnerKkJapyWoIyVSwoE0NK0TbfYTCS_U4y7yT7nWS_U-b3fzb4pr-GyQBZAP2z8r51VtvY_WP7CeQfniU</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Soares, Tânia Cristina Benetti</creator><creator>Isaias, Gustavo Alves Brito</creator><creator>Almeida, Amanda Roberta de</creator><creator>Drummond, Marina Rovani</creator><creator>da Silva, Marilene Neves</creator><creator>Lania, Bruno Grosselli</creator><creator>Vieira-Damiani, Gislaine</creator><creator>Saad, Sara Teresinha Olalla</creator><creator>Ericson, Marna Elise</creator><creator>Gupta, Kalpna</creator><creator>Velho, Paulo Eduardo Neves Ferreira</creator><general>Mary Ann Liebert, Inc., publishers</general><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SS</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200701</creationdate><title>Prevalence of Bartonella spp. Infection in Patients with Sickle Cell Disease</title><author>Soares, Tânia Cristina Benetti ; Isaias, Gustavo Alves Brito ; Almeida, Amanda Roberta de ; Drummond, Marina Rovani ; da Silva, Marilene Neves ; Lania, Bruno Grosselli ; Vieira-Damiani, Gislaine ; Saad, Sara Teresinha Olalla ; Ericson, Marna Elise ; Gupta, Kalpna ; Velho, Paulo Eduardo Neves Ferreira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-8605b4cfb77888f7cb47089db5810605620244c7fb50fcc37508c64b141069483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Amplification</topic><topic>Anemia, Sickle Cell - complications</topic><topic>Bartonella</topic><topic>Bartonella - isolation & purification</topic><topic>Bartonella Infections - complications</topic><topic>Bartonella Infections - epidemiology</topic><topic>Blood transfusion</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>FtsZ gene</topic><topic>Hematology</topic><topic>Humans</topic><topic>Immunodeficiency</topic><topic>Infections</topic><topic>Original</topic><topic>Original Articles</topic><topic>Patients</topic><topic>Polymerase Chain Reaction</topic><topic>Prevalence</topic><topic>Risk Factors</topic><topic>Sequences</topic><topic>Sickle cell disease</topic><topic>Spacer region</topic><topic>Statistical methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soares, Tânia Cristina Benetti</creatorcontrib><creatorcontrib>Isaias, Gustavo Alves Brito</creatorcontrib><creatorcontrib>Almeida, Amanda Roberta de</creatorcontrib><creatorcontrib>Drummond, Marina Rovani</creatorcontrib><creatorcontrib>da Silva, Marilene Neves</creatorcontrib><creatorcontrib>Lania, Bruno Grosselli</creatorcontrib><creatorcontrib>Vieira-Damiani, Gislaine</creatorcontrib><creatorcontrib>Saad, Sara Teresinha Olalla</creatorcontrib><creatorcontrib>Ericson, Marna Elise</creatorcontrib><creatorcontrib>Gupta, Kalpna</creatorcontrib><creatorcontrib>Velho, Paulo Eduardo Neves Ferreira</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soares, Tânia Cristina Benetti</au><au>Isaias, Gustavo Alves Brito</au><au>Almeida, Amanda Roberta de</au><au>Drummond, Marina Rovani</au><au>da Silva, Marilene Neves</au><au>Lania, Bruno Grosselli</au><au>Vieira-Damiani, Gislaine</au><au>Saad, Sara Teresinha Olalla</au><au>Ericson, Marna Elise</au><au>Gupta, Kalpna</au><au>Velho, Paulo Eduardo Neves Ferreira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence of Bartonella spp. Infection in Patients with Sickle Cell Disease</atitle><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle><addtitle>Vector Borne Zoonotic Dis</addtitle><date>2020-07-01</date><risdate>2020</risdate><volume>20</volume><issue>7</issue><spage>59</spage><epage>512</epage><pages>59-512</pages><issn>1530-3667</issn><eissn>1557-7759</eissn><abstract>Background:
The inherent characteristics of the sickle cell disease (SCD), the most common genetic hematological disorder, increase the propensity of infections.
Bartonella
spp. are emerging and neglected bacteria. A large spectrum of clinical manifestations has been linked to bartonella bloodstream infection in the last two decades that can cause fatal outcomes, especially in immunodeficient patients. The goal of this study was to evaluate the prevalence of bartonella infection in SCD patients.
Materials and Methods:
We evaluated
Bartonella
spp. prevalence in 107 SCD patients. Blood samples and enrichment blood cultures were analyzed by molecular detection of
Bartonella
spp. DNA. Bartonella DNA was amplified using conventional genus-specific
Bartonella
PCR which amplifies the Intergenic Transcribed Spacer region and
Bartonella henselae-
specific nested PCR which amplifies the
FtsZ
gene. Positive patient DNAs were tested with ssrA conventional PCR. All amplicons were sequenced.
Findings:
Ten of 107 patients tested positive for
B. henselae
infection in at least one molecular test. All obtained amplicons were sequenced and similar to
B. henselae
sequences deposited in GenBank (accession number BX897699). Based on statistical results, bloodstream infection with
B. henselae
was not associated with animal contact or blood transfusions.
Conclusion:
We detected
B. henselae
DNA in 10 (9.3%) SCD studied patients. These patients were notified and treatment was offered to them.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>32013778</pmid><doi>10.1089/vbz.2019.2545</doi><tpages>454</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1530-3667 |
| ispartof | Vector borne and zoonotic diseases (Larchmont, N.Y.), 2020-07, Vol.20 (7), p.59-512 |
| issn | 1530-3667 1557-7759 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7336878 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Amplification Anemia, Sickle Cell - complications Bartonella Bartonella - isolation & purification Bartonella Infections - complications Bartonella Infections - epidemiology Blood transfusion Deoxyribonucleic acid DNA FtsZ gene Hematology Humans Immunodeficiency Infections Original Original Articles Patients Polymerase Chain Reaction Prevalence Risk Factors Sequences Sickle cell disease Spacer region Statistical methods |
| title | Prevalence of Bartonella spp. Infection in Patients with Sickle Cell Disease |
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