TWIST-1 Is Overexpressed in Neoplastic Choroid Plexus Epithelial Cells and Promotes Proliferation and Invasion
The pathogenesis of choroid plexus papillomas, intraventricular papillary neoplasms most often occurring sporadically in children and young adults, remains poorly understood. To identify pathways operative in the development of choroid plexus papillomas, gene expression profiles obtained from laser-...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 2009-03, Vol.69 (6), p.2219-2223 |
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| creator | HASSELBLATT, Martin MERTSCH, Sonja WEI ZHENG PAULUS, Werner KOOS, Björn RIESMEIER, Barbara STEGEMANN, Heike JEIBMANN, Astrid TOMM, Manuel SCHMITZ, Nicole WREDE, Brigitte WOLFF, Johannes E |
| description | The pathogenesis of choroid plexus papillomas, intraventricular papillary neoplasms most often occurring sporadically in children and young adults, remains poorly understood. To identify pathways operative in the development of choroid plexus papillomas, gene expression profiles obtained from laser-microdissected human choroid plexus papilloma cells (n = 7) were compared with that of normal choroid plexus epithelial cells laser microdissected from autopsy tissue (n = 8). On DNA microarray data analysis, 53 probe sets were differentially expressed in choroid plexus papilloma tumor cells (>7-fold). Up-regulation of TWIST1, WIF1, TRPM3, BCLAF1, and AJAP1, as well as down-regulation of IL6ST was confirmed using quantitative reverse transcription-PCR. Knockdown of Twist1 gene expression in the rat choroid plexus epithelial cell line Z310 significantly reduced proliferation as assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cell invasion in a Matrigel assay, whereas cell migration was not affected. Screening for expressional changes of cancer-related genes upon Twist1 knockdown revealed up-regulation of Cdkn1a, Cflar, and Serpinb2 and down-regulation of Figf. To conclude, using gene expression profiling, several genes differentially expressed in human choroid plexus papillomas could be identified. Among those, TWIST1 is highly expressed in choroid plexus papillomas and promotes proliferation and invasion. |
| doi_str_mv | 10.1158/0008-5472.CAN-08-3176 |
| format | Article |
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To identify pathways operative in the development of choroid plexus papillomas, gene expression profiles obtained from laser-microdissected human choroid plexus papilloma cells (n = 7) were compared with that of normal choroid plexus epithelial cells laser microdissected from autopsy tissue (n = 8). On DNA microarray data analysis, 53 probe sets were differentially expressed in choroid plexus papilloma tumor cells (>7-fold). Up-regulation of TWIST1, WIF1, TRPM3, BCLAF1, and AJAP1, as well as down-regulation of IL6ST was confirmed using quantitative reverse transcription-PCR. Knockdown of Twist1 gene expression in the rat choroid plexus epithelial cell line Z310 significantly reduced proliferation as assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cell invasion in a Matrigel assay, whereas cell migration was not affected. Screening for expressional changes of cancer-related genes upon Twist1 knockdown revealed up-regulation of Cdkn1a, Cflar, and Serpinb2 and down-regulation of Figf. To conclude, using gene expression profiling, several genes differentially expressed in human choroid plexus papillomas could be identified. Among those, TWIST1 is highly expressed in choroid plexus papillomas and promotes proliferation and invasion.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>DOI: 10.1158/0008-5472.CAN-08-3176</identifier><identifier>PMID: 19276370</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Adolescent ; Adult ; Animals ; Antineoplastic agents ; Biological and medical sciences ; Cell Growth Processes - physiology ; Cell Movement - physiology ; Child ; Child, Preschool ; Epithelial Cells - metabolism ; Epithelial Cells - pathology ; Female ; Gene Expression Profiling ; Humans ; Infant ; Infant, Newborn ; Male ; Medical sciences ; Microdissection ; Middle Aged ; Neoplasm Invasiveness ; Nuclear Proteins - biosynthesis ; Nuclear Proteins - genetics ; Papilloma, Choroid Plexus - genetics ; Papilloma, Choroid Plexus - metabolism ; Papilloma, Choroid Plexus - pathology ; Pharmacology. Drug treatments ; Rats ; Tumors ; Twist-Related Protein 1 - biosynthesis ; Twist-Related Protein 1 - genetics ; Young Adult</subject><ispartof>Cancer research (Chicago, Ill.), 2009-03, Vol.69 (6), p.2219-2223</ispartof><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-d41e9ea62c44f19751bc0e1eecb2a74d9bf927dcdcb74bae9ad72cb9742625b33</citedby><cites>FETCH-LOGICAL-c472t-d41e9ea62c44f19751bc0e1eecb2a74d9bf927dcdcb74bae9ad72cb9742625b33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,3343,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21883606$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19276370$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HASSELBLATT, Martin</creatorcontrib><creatorcontrib>MERTSCH, Sonja</creatorcontrib><creatorcontrib>WEI ZHENG</creatorcontrib><creatorcontrib>PAULUS, Werner</creatorcontrib><creatorcontrib>KOOS, Björn</creatorcontrib><creatorcontrib>RIESMEIER, Barbara</creatorcontrib><creatorcontrib>STEGEMANN, Heike</creatorcontrib><creatorcontrib>JEIBMANN, Astrid</creatorcontrib><creatorcontrib>TOMM, Manuel</creatorcontrib><creatorcontrib>SCHMITZ, Nicole</creatorcontrib><creatorcontrib>WREDE, Brigitte</creatorcontrib><creatorcontrib>WOLFF, Johannes E</creatorcontrib><title>TWIST-1 Is Overexpressed in Neoplastic Choroid Plexus Epithelial Cells and Promotes Proliferation and Invasion</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>The pathogenesis of choroid plexus papillomas, intraventricular papillary neoplasms most often occurring sporadically in children and young adults, remains poorly understood. To identify pathways operative in the development of choroid plexus papillomas, gene expression profiles obtained from laser-microdissected human choroid plexus papilloma cells (n = 7) were compared with that of normal choroid plexus epithelial cells laser microdissected from autopsy tissue (n = 8). On DNA microarray data analysis, 53 probe sets were differentially expressed in choroid plexus papilloma tumor cells (>7-fold). Up-regulation of TWIST1, WIF1, TRPM3, BCLAF1, and AJAP1, as well as down-regulation of IL6ST was confirmed using quantitative reverse transcription-PCR. Knockdown of Twist1 gene expression in the rat choroid plexus epithelial cell line Z310 significantly reduced proliferation as assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cell invasion in a Matrigel assay, whereas cell migration was not affected. Screening for expressional changes of cancer-related genes upon Twist1 knockdown revealed up-regulation of Cdkn1a, Cflar, and Serpinb2 and down-regulation of Figf. To conclude, using gene expression profiling, several genes differentially expressed in human choroid plexus papillomas could be identified. Among those, TWIST1 is highly expressed in choroid plexus papillomas and promotes proliferation and invasion.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Animals</subject><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>Cell Growth Processes - physiology</subject><subject>Cell Movement - physiology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - pathology</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microdissection</subject><subject>Middle Aged</subject><subject>Neoplasm Invasiveness</subject><subject>Nuclear Proteins - biosynthesis</subject><subject>Nuclear Proteins - genetics</subject><subject>Papilloma, Choroid Plexus - genetics</subject><subject>Papilloma, Choroid Plexus - metabolism</subject><subject>Papilloma, Choroid Plexus - pathology</subject><subject>Pharmacology. Drug treatments</subject><subject>Rats</subject><subject>Tumors</subject><subject>Twist-Related Protein 1 - biosynthesis</subject><subject>Twist-Related Protein 1 - genetics</subject><subject>Young Adult</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU9v1DAQxS0EokvhI4B8obcU_42TC1IVFbpS1SKxiKPlOBPWyGsHO7sq3x6nXS1w8hu9N-MZ_RB6S8klpbL5QAhpKikUu-yu7qqiOVX1M7SikjeVEkI-R6tT5gy9yvlnKSUl8iU6oy1TNVdkhcLm-_rrpqJ4nfH9ARI8TAlyhgG7gO8gTt7k2VncbWOKbsBfPDzsM76e3LwF74zHHXifsQnFS3EXZ8iL8G6EZGYXw6O1DgeTS_EavRiNz_Dm-J6jb5-uN91NdXv_ed1d3Va2bDtXg6DQgqmZFWKkrZK0twQogO2ZUWJo-7FcMNjB9kr0BlozKGb7VglWM9lzfo4-Ps2d9v0OBgthTsbrKbmdSb91NE7_7wS31T_iQSvOpahZGXBxHJDirz3kWe9ctuVUEyDus64VEVTSpgTlU9CmmHOC8fQJJXohpRcKeqGgCyld9EKq9L37d8O_XUc0JfD-GDDZGj8mE6zLpxyjTcNrUvM_GU-fJw</recordid><startdate>20090315</startdate><enddate>20090315</enddate><creator>HASSELBLATT, Martin</creator><creator>MERTSCH, Sonja</creator><creator>WEI ZHENG</creator><creator>PAULUS, Werner</creator><creator>KOOS, Björn</creator><creator>RIESMEIER, Barbara</creator><creator>STEGEMANN, Heike</creator><creator>JEIBMANN, Astrid</creator><creator>TOMM, Manuel</creator><creator>SCHMITZ, Nicole</creator><creator>WREDE, Brigitte</creator><creator>WOLFF, Johannes E</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090315</creationdate><title>TWIST-1 Is Overexpressed in Neoplastic Choroid Plexus Epithelial Cells and Promotes Proliferation and Invasion</title><author>HASSELBLATT, Martin ; MERTSCH, Sonja ; WEI ZHENG ; PAULUS, Werner ; KOOS, Björn ; RIESMEIER, Barbara ; STEGEMANN, Heike ; JEIBMANN, Astrid ; TOMM, Manuel ; SCHMITZ, Nicole ; WREDE, Brigitte ; WOLFF, Johannes E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-d41e9ea62c44f19751bc0e1eecb2a74d9bf927dcdcb74bae9ad72cb9742625b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Animals</topic><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>Cell Growth Processes - physiology</topic><topic>Cell Movement - physiology</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial Cells - pathology</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microdissection</topic><topic>Middle Aged</topic><topic>Neoplasm Invasiveness</topic><topic>Nuclear Proteins - biosynthesis</topic><topic>Nuclear Proteins - genetics</topic><topic>Papilloma, Choroid Plexus - genetics</topic><topic>Papilloma, Choroid Plexus - metabolism</topic><topic>Papilloma, Choroid Plexus - pathology</topic><topic>Pharmacology. 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To identify pathways operative in the development of choroid plexus papillomas, gene expression profiles obtained from laser-microdissected human choroid plexus papilloma cells (n = 7) were compared with that of normal choroid plexus epithelial cells laser microdissected from autopsy tissue (n = 8). On DNA microarray data analysis, 53 probe sets were differentially expressed in choroid plexus papilloma tumor cells (>7-fold). Up-regulation of TWIST1, WIF1, TRPM3, BCLAF1, and AJAP1, as well as down-regulation of IL6ST was confirmed using quantitative reverse transcription-PCR. Knockdown of Twist1 gene expression in the rat choroid plexus epithelial cell line Z310 significantly reduced proliferation as assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cell invasion in a Matrigel assay, whereas cell migration was not affected. Screening for expressional changes of cancer-related genes upon Twist1 knockdown revealed up-regulation of Cdkn1a, Cflar, and Serpinb2 and down-regulation of Figf. To conclude, using gene expression profiling, several genes differentially expressed in human choroid plexus papillomas could be identified. Among those, TWIST1 is highly expressed in choroid plexus papillomas and promotes proliferation and invasion.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>19276370</pmid><doi>10.1158/0008-5472.CAN-08-3176</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Animals Antineoplastic agents Biological and medical sciences Cell Growth Processes - physiology Cell Movement - physiology Child Child, Preschool Epithelial Cells - metabolism Epithelial Cells - pathology Female Gene Expression Profiling Humans Infant Infant, Newborn Male Medical sciences Microdissection Middle Aged Neoplasm Invasiveness Nuclear Proteins - biosynthesis Nuclear Proteins - genetics Papilloma, Choroid Plexus - genetics Papilloma, Choroid Plexus - metabolism Papilloma, Choroid Plexus - pathology Pharmacology. Drug treatments Rats Tumors Twist-Related Protein 1 - biosynthesis Twist-Related Protein 1 - genetics Young Adult |
| title | TWIST-1 Is Overexpressed in Neoplastic Choroid Plexus Epithelial Cells and Promotes Proliferation and Invasion |
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