Increased Responses of Phenoloxidase in Chlorantraniliprole Resistance of Plutella xylostella (Lepidoptera: Plutellidae)
Abstract The diamondback moth (Plutella xylostella, DBM) is an important pest of cruciferous vegetables. The use of chlorantraniliprole has been essential in the management of the DBM. However, in many countries and areas, DBM has become highly resistant to chlorantraniliprole. Three different DBM s...
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| Veröffentlicht in: | Journal of insect science (Tucson, Ariz.) Ariz.), 2020-07, Vol.20 (4) |
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| creator | Wang, Nian-Meng Li, Jing-Jing Shang, Ze-Yu Yu, Qi-Tong Xue, Chao-Bin |
| description | Abstract
The diamondback moth (Plutella xylostella, DBM) is an important pest of cruciferous vegetables. The use of chlorantraniliprole has been essential in the management of the DBM. However, in many countries and areas, DBM has become highly resistant to chlorantraniliprole. Three different DBM strains, susceptible (S), chlorantraniliprole-selected (Rc), and field-collected (Rb) resistant strains/populations were studied for the role of phenoloxidase in resistance development to the insecticide. By assaying the activity of phenoloxidase (PO) in the three different DBM strains, the results showed that the PO activity in the Rc strain was increased significantly compared with the S strain. The synergistic effects of quercetin showed that the resistant ratio (RR) of the QRc larvae to chlorantraniliprole was decreased from 423.95 to 316.42-fold compared with the Rc larvae. Further studies demonstrated that the transcriptional and translational expression levels of PxPPO1 (P. xylostella prophenoloxidase-1 gene) and PxPPO2 (P. xylostella prophenoloxidase-2 gene) were increased to varying degrees compared with the S strain, such as the transcriptional expression levels of PxPPO2 were 24.02-fold that of the S strain. The responses of phenoloxidase were significantly different in chlorantraniliprole-resistant DBM.
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| doi_str_mv | 10.1093/jisesa/ieaa066 |
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The diamondback moth (Plutella xylostella, DBM) is an important pest of cruciferous vegetables. The use of chlorantraniliprole has been essential in the management of the DBM. However, in many countries and areas, DBM has become highly resistant to chlorantraniliprole. Three different DBM strains, susceptible (S), chlorantraniliprole-selected (Rc), and field-collected (Rb) resistant strains/populations were studied for the role of phenoloxidase in resistance development to the insecticide. By assaying the activity of phenoloxidase (PO) in the three different DBM strains, the results showed that the PO activity in the Rc strain was increased significantly compared with the S strain. The synergistic effects of quercetin showed that the resistant ratio (RR) of the QRc larvae to chlorantraniliprole was decreased from 423.95 to 316.42-fold compared with the Rc larvae. Further studies demonstrated that the transcriptional and translational expression levels of PxPPO1 (P. xylostella prophenoloxidase-1 gene) and PxPPO2 (P. xylostella prophenoloxidase-2 gene) were increased to varying degrees compared with the S strain, such as the transcriptional expression levels of PxPPO2 were 24.02-fold that of the S strain. The responses of phenoloxidase were significantly different in chlorantraniliprole-resistant DBM.
Graphical Abstract</description><identifier>ISSN: 1536-2442</identifier><identifier>EISSN: 1536-2442</identifier><identifier>DOI: 10.1093/jisesa/ieaa066</identifier><identifier>PMID: 32620012</identifier><language>eng</language><publisher>US: Oxford University Press</publisher><subject>Animals ; Control ; Diamond-back moth ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Insecticide resistance ; Insecticide Resistance - genetics ; Larva - drug effects ; Larva - enzymology ; Larva - genetics ; Larva - growth & development ; Monophenol Monooxygenase - genetics ; Monophenol Monooxygenase - metabolism ; Moths - drug effects ; Moths - enzymology ; Moths - genetics ; Moths - growth & development ; ortho-Aminobenzoates - pharmacology ; Oxidases ; Physiological aspects ; Zoological research</subject><ispartof>Journal of insect science (Tucson, Ariz.), 2020-07, Vol.20 (4)</ispartof><rights>The Author(s) 2020. Published by Oxford University Press on behalf of Entomological Society of America. 2020</rights><rights>The Author(s) 2020. Published by Oxford University Press on behalf of Entomological Society of America.</rights><rights>COPYRIGHT 2020 Oxford University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-f670e94ac037e7c7a6c0948e02a001c162d0059c9a01c3fde341003f900780303</citedby><cites>FETCH-LOGICAL-c493t-f670e94ac037e7c7a6c0948e02a001c162d0059c9a01c3fde341003f900780303</cites><orcidid>0000-0001-6891-7120</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334004/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334004/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1598,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32620012$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zhu, Yu-Cheng</contributor><creatorcontrib>Wang, Nian-Meng</creatorcontrib><creatorcontrib>Li, Jing-Jing</creatorcontrib><creatorcontrib>Shang, Ze-Yu</creatorcontrib><creatorcontrib>Yu, Qi-Tong</creatorcontrib><creatorcontrib>Xue, Chao-Bin</creatorcontrib><title>Increased Responses of Phenoloxidase in Chlorantraniliprole Resistance of Plutella xylostella (Lepidoptera: Plutellidae)</title><title>Journal of insect science (Tucson, Ariz.)</title><addtitle>J Insect Sci</addtitle><description>Abstract
The diamondback moth (Plutella xylostella, DBM) is an important pest of cruciferous vegetables. The use of chlorantraniliprole has been essential in the management of the DBM. However, in many countries and areas, DBM has become highly resistant to chlorantraniliprole. Three different DBM strains, susceptible (S), chlorantraniliprole-selected (Rc), and field-collected (Rb) resistant strains/populations were studied for the role of phenoloxidase in resistance development to the insecticide. By assaying the activity of phenoloxidase (PO) in the three different DBM strains, the results showed that the PO activity in the Rc strain was increased significantly compared with the S strain. The synergistic effects of quercetin showed that the resistant ratio (RR) of the QRc larvae to chlorantraniliprole was decreased from 423.95 to 316.42-fold compared with the Rc larvae. Further studies demonstrated that the transcriptional and translational expression levels of PxPPO1 (P. xylostella prophenoloxidase-1 gene) and PxPPO2 (P. xylostella prophenoloxidase-2 gene) were increased to varying degrees compared with the S strain, such as the transcriptional expression levels of PxPPO2 were 24.02-fold that of the S strain. The responses of phenoloxidase were significantly different in chlorantraniliprole-resistant DBM.
Graphical Abstract</description><subject>Animals</subject><subject>Control</subject><subject>Diamond-back moth</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Insecticide resistance</subject><subject>Insecticide Resistance - genetics</subject><subject>Larva - drug effects</subject><subject>Larva - enzymology</subject><subject>Larva - genetics</subject><subject>Larva - growth & development</subject><subject>Monophenol Monooxygenase - genetics</subject><subject>Monophenol Monooxygenase - metabolism</subject><subject>Moths - drug effects</subject><subject>Moths - enzymology</subject><subject>Moths - genetics</subject><subject>Moths - growth & development</subject><subject>ortho-Aminobenzoates - pharmacology</subject><subject>Oxidases</subject><subject>Physiological aspects</subject><subject>Zoological research</subject><issn>1536-2442</issn><issn>1536-2442</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>TOX</sourceid><sourceid>EIF</sourceid><recordid>eNqFkUtvGyEUhVHVqnlus6xm2Syc3AHMmC4qRVYeliy1SpM1umHu2EQYRsM4cv59cOw8vKoQ4gLnfHB1GDsp4awELc4fXaKE544QQakvbL8cCjXgUvKvn-o9dpDSIwAHOdLf2Z7gigOUfJ-tJsF2hInq4pZSG0PGFbEp_s4pRB9Xrs53hQvFeO5jh6HP03nXdtHT2uFSj8HSq8Uve_Iei9Wzj2lT_pxS6-rY9tThrzdFZtLpEfvWoE90vF0P2f3V5d34ZjD9cz0ZX0wHVmrRDxpVAWmJFkRFla1QWdByRMAxN2BLxWuAobYa8040NQlZAohGA1QjECAO2e8Nt10-LKi2tG7Bm7ZzC-yeTURndm-Cm5tZfDKVEBJAZsDZBjBDT8aFJmaZzaOmhbMxUOPy-YWSaqi41iobTncMWdPTqp_hMiUz-Xe7q93CbRdT6qh5_1gJZh2w2QRstgFnw4_P7bzL3xL9eD0u2__BXgD5kbQC</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Wang, Nian-Meng</creator><creator>Li, Jing-Jing</creator><creator>Shang, Ze-Yu</creator><creator>Yu, Qi-Tong</creator><creator>Xue, Chao-Bin</creator><general>Oxford University Press</general><scope>TOX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6891-7120</orcidid></search><sort><creationdate>20200701</creationdate><title>Increased Responses of Phenoloxidase in Chlorantraniliprole Resistance of Plutella xylostella (Lepidoptera: Plutellidae)</title><author>Wang, Nian-Meng ; Li, Jing-Jing ; Shang, Ze-Yu ; Yu, Qi-Tong ; Xue, Chao-Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-f670e94ac037e7c7a6c0948e02a001c162d0059c9a01c3fde341003f900780303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Control</topic><topic>Diamond-back moth</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>Insecticide resistance</topic><topic>Insecticide Resistance - genetics</topic><topic>Larva - drug effects</topic><topic>Larva - enzymology</topic><topic>Larva - genetics</topic><topic>Larva - growth & development</topic><topic>Monophenol Monooxygenase - genetics</topic><topic>Monophenol Monooxygenase - metabolism</topic><topic>Moths - drug effects</topic><topic>Moths - enzymology</topic><topic>Moths - genetics</topic><topic>Moths - growth & development</topic><topic>ortho-Aminobenzoates - pharmacology</topic><topic>Oxidases</topic><topic>Physiological aspects</topic><topic>Zoological research</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Nian-Meng</creatorcontrib><creatorcontrib>Li, Jing-Jing</creatorcontrib><creatorcontrib>Shang, Ze-Yu</creatorcontrib><creatorcontrib>Yu, Qi-Tong</creatorcontrib><creatorcontrib>Xue, Chao-Bin</creatorcontrib><collection>Oxford Journals Open Access Collection</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of insect science (Tucson, Ariz.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Nian-Meng</au><au>Li, Jing-Jing</au><au>Shang, Ze-Yu</au><au>Yu, Qi-Tong</au><au>Xue, Chao-Bin</au><au>Zhu, Yu-Cheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased Responses of Phenoloxidase in Chlorantraniliprole Resistance of Plutella xylostella (Lepidoptera: Plutellidae)</atitle><jtitle>Journal of insect science (Tucson, Ariz.)</jtitle><addtitle>J Insect Sci</addtitle><date>2020-07-01</date><risdate>2020</risdate><volume>20</volume><issue>4</issue><issn>1536-2442</issn><eissn>1536-2442</eissn><abstract>Abstract
The diamondback moth (Plutella xylostella, DBM) is an important pest of cruciferous vegetables. The use of chlorantraniliprole has been essential in the management of the DBM. However, in many countries and areas, DBM has become highly resistant to chlorantraniliprole. Three different DBM strains, susceptible (S), chlorantraniliprole-selected (Rc), and field-collected (Rb) resistant strains/populations were studied for the role of phenoloxidase in resistance development to the insecticide. By assaying the activity of phenoloxidase (PO) in the three different DBM strains, the results showed that the PO activity in the Rc strain was increased significantly compared with the S strain. The synergistic effects of quercetin showed that the resistant ratio (RR) of the QRc larvae to chlorantraniliprole was decreased from 423.95 to 316.42-fold compared with the Rc larvae. Further studies demonstrated that the transcriptional and translational expression levels of PxPPO1 (P. xylostella prophenoloxidase-1 gene) and PxPPO2 (P. xylostella prophenoloxidase-2 gene) were increased to varying degrees compared with the S strain, such as the transcriptional expression levels of PxPPO2 were 24.02-fold that of the S strain. The responses of phenoloxidase were significantly different in chlorantraniliprole-resistant DBM.
Graphical Abstract</abstract><cop>US</cop><pub>Oxford University Press</pub><pmid>32620012</pmid><doi>10.1093/jisesa/ieaa066</doi><orcidid>https://orcid.org/0000-0001-6891-7120</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Oxford Journals Open Access Collection; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Control Diamond-back moth Insect Proteins - genetics Insect Proteins - metabolism Insecticide resistance Insecticide Resistance - genetics Larva - drug effects Larva - enzymology Larva - genetics Larva - growth & development Monophenol Monooxygenase - genetics Monophenol Monooxygenase - metabolism Moths - drug effects Moths - enzymology Moths - genetics Moths - growth & development ortho-Aminobenzoates - pharmacology Oxidases Physiological aspects Zoological research |
| title | Increased Responses of Phenoloxidase in Chlorantraniliprole Resistance of Plutella xylostella (Lepidoptera: Plutellidae) |
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