Antinociceptive Effects of Shenling Baizhu through PI3K-Akt-mTOR Signaling Pathway in a Mouse Model of Bone Metastasis with Small-Cell Lung Cancer
Shenling Baizhu additive powder (SLBZ-AP), a formulation of a variety of natural medicinal plants, has clinical efficacy in treating cancers in previous studies. We explored the effect of SLBZ-AP in bone metastasis of lung cancer (BMLC) mice, and the possible mechanism involved was further investiga...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2020, Vol.2020 (2020), p.1-12 |
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| creator | Huang, Peng Huang, Long Cui, Zhenwei Feng, Sitan Feng, XinJian Mo, Jian Su, Bo Cheng, Weimin Han, Jie Feng, Ziyi Feng, Zhe Chen, GuoJian |
| description | Shenling Baizhu additive powder (SLBZ-AP), a formulation of a variety of natural medicinal plants, has clinical efficacy in treating cancers in previous studies. We explored the effect of SLBZ-AP in bone metastasis of lung cancer (BMLC) mice, and the possible mechanism involved was further investigated in the present study. Mice model of BMLC was made and treated with SLBZ-AP. Pain behavioral tests were performed to explore the effect on BMLC-induced pain in mice. TUNEL staining was used to investigate apoptosis. The mRNA expression of markers in the PI3K/Akt/mTOR pathway was measured by qPCR, and protein expression was detected by western blotting and immunohistochemistry analysis. SLBZ-AP relieved BMLC-induced pain and prolonged animals’ survival, promoted cell apoptosis in the marrow from the tibia of BMLC mice, and inhibited mRNA and protein expression of AKT, mTOR, P70S6, and VEGF, as well as protein expression of p-AKT, p-mTOR, p-P70S6, and VEGF upregulation in the marrow of tibia induced by BMLC, an effect which was similar to rapamycin. Our results suggested that SLBZ-AP may have antinociceptive effect and prolong survival of BMLC mice at least partially by inhibiting cell proliferation and promoting apoptosis through the PI3K/Akt/mTOR signaling pathway. SLBZ-AP may be a potential candidate for BMLC therapy. |
| doi_str_mv | 10.1155/2020/4121483 |
| format | Article |
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We explored the effect of SLBZ-AP in bone metastasis of lung cancer (BMLC) mice, and the possible mechanism involved was further investigated in the present study. Mice model of BMLC was made and treated with SLBZ-AP. Pain behavioral tests were performed to explore the effect on BMLC-induced pain in mice. TUNEL staining was used to investigate apoptosis. The mRNA expression of markers in the PI3K/Akt/mTOR pathway was measured by qPCR, and protein expression was detected by western blotting and immunohistochemistry analysis. SLBZ-AP relieved BMLC-induced pain and prolonged animals’ survival, promoted cell apoptosis in the marrow from the tibia of BMLC mice, and inhibited mRNA and protein expression of AKT, mTOR, P70S6, and VEGF, as well as protein expression of p-AKT, p-mTOR, p-P70S6, and VEGF upregulation in the marrow of tibia induced by BMLC, an effect which was similar to rapamycin. Our results suggested that SLBZ-AP may have antinociceptive effect and prolong survival of BMLC mice at least partially by inhibiting cell proliferation and promoting apoptosis through the PI3K/Akt/mTOR signaling pathway. SLBZ-AP may be a potential candidate for BMLC therapy.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2020/4121483</identifier><identifier>PMID: 32655659</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>1-Phosphatidylinositol 3-kinase ; AKT protein ; Analysis ; Animals ; Apoptosis ; Bone cancer ; Cancer ; Cancer therapies ; Care and treatment ; Cell proliferation ; Cell survival ; Chinese medicine ; Drug dosages ; Drug withdrawal ; Gene expression ; Immunohistochemistry ; Lung cancer ; Medical prognosis ; Medicinal plants ; Medicine, Botanic ; Medicine, Herbal ; Metastases ; Metastasis ; Oncology, Experimental ; Pain ; Pain perception ; Pharmacy ; Quality of life ; Rapamycin ; Ribosomal protein S6 kinase ; RNA ; Signal transduction ; Small cell lung carcinoma ; Tibia ; TOR protein ; Vascular endothelial growth factor ; Western blotting</subject><ispartof>Evidence-based complementary and alternative medicine, 2020, Vol.2020 (2020), p.1-12</ispartof><rights>Copyright © 2020 Zhe Feng et al.</rights><rights>COPYRIGHT 2020 John Wiley & Sons, Inc.</rights><rights>Copyright © 2020 Zhe Feng et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2020 Zhe Feng et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-c6f007c10d9beab635a46ef98e0943ff589a4f373f98f00eb919ab8ec96f5da03</citedby><cites>FETCH-LOGICAL-c476t-c6f007c10d9beab635a46ef98e0943ff589a4f373f98f00eb919ab8ec96f5da03</cites><orcidid>0000-0003-0069-2564 ; 0000-0003-1750-4400 ; 0000-0001-5526-0238 ; 0000-0003-3299-4567 ; 0000-0002-2430-8312 ; 0000-0001-8261-5559 ; 0000-0002-7309-8083 ; 0000-0002-4206-5385 ; 0000-0001-5625-1501 ; 0000-0002-9923-7774 ; 0000-0002-1942-3105</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327581/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327581/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids></links><search><contributor>Makino, Toshiaki</contributor><creatorcontrib>Huang, Peng</creatorcontrib><creatorcontrib>Huang, Long</creatorcontrib><creatorcontrib>Cui, Zhenwei</creatorcontrib><creatorcontrib>Feng, Sitan</creatorcontrib><creatorcontrib>Feng, XinJian</creatorcontrib><creatorcontrib>Mo, Jian</creatorcontrib><creatorcontrib>Su, Bo</creatorcontrib><creatorcontrib>Cheng, Weimin</creatorcontrib><creatorcontrib>Han, Jie</creatorcontrib><creatorcontrib>Feng, Ziyi</creatorcontrib><creatorcontrib>Feng, Zhe</creatorcontrib><creatorcontrib>Chen, GuoJian</creatorcontrib><title>Antinociceptive Effects of Shenling Baizhu through PI3K-Akt-mTOR Signaling Pathway in a Mouse Model of Bone Metastasis with Small-Cell Lung Cancer</title><title>Evidence-based complementary and alternative medicine</title><description>Shenling Baizhu additive powder (SLBZ-AP), a formulation of a variety of natural medicinal plants, has clinical efficacy in treating cancers in previous studies. We explored the effect of SLBZ-AP in bone metastasis of lung cancer (BMLC) mice, and the possible mechanism involved was further investigated in the present study. Mice model of BMLC was made and treated with SLBZ-AP. Pain behavioral tests were performed to explore the effect on BMLC-induced pain in mice. TUNEL staining was used to investigate apoptosis. The mRNA expression of markers in the PI3K/Akt/mTOR pathway was measured by qPCR, and protein expression was detected by western blotting and immunohistochemistry analysis. SLBZ-AP relieved BMLC-induced pain and prolonged animals’ survival, promoted cell apoptosis in the marrow from the tibia of BMLC mice, and inhibited mRNA and protein expression of AKT, mTOR, P70S6, and VEGF, as well as protein expression of p-AKT, p-mTOR, p-P70S6, and VEGF upregulation in the marrow of tibia induced by BMLC, an effect which was similar to rapamycin. Our results suggested that SLBZ-AP may have antinociceptive effect and prolong survival of BMLC mice at least partially by inhibiting cell proliferation and promoting apoptosis through the PI3K/Akt/mTOR signaling pathway. SLBZ-AP may be a potential candidate for BMLC therapy.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>AKT protein</subject><subject>Analysis</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Bone cancer</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Care and treatment</subject><subject>Cell proliferation</subject><subject>Cell survival</subject><subject>Chinese medicine</subject><subject>Drug dosages</subject><subject>Drug withdrawal</subject><subject>Gene expression</subject><subject>Immunohistochemistry</subject><subject>Lung cancer</subject><subject>Medical prognosis</subject><subject>Medicinal plants</subject><subject>Medicine, Botanic</subject><subject>Medicine, Herbal</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Oncology, Experimental</subject><subject>Pain</subject><subject>Pain perception</subject><subject>Pharmacy</subject><subject>Quality of life</subject><subject>Rapamycin</subject><subject>Ribosomal protein S6 kinase</subject><subject>RNA</subject><subject>Signal transduction</subject><subject>Small cell lung carcinoma</subject><subject>Tibia</subject><subject>TOR protein</subject><subject>Vascular endothelial growth factor</subject><subject>Western 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Effects of Shenling Baizhu through PI3K-Akt-mTOR Signaling Pathway in a Mouse Model of Bone Metastasis with Small-Cell Lung Cancer</title><author>Huang, Peng ; Huang, Long ; Cui, Zhenwei ; Feng, Sitan ; Feng, XinJian ; Mo, Jian ; Su, Bo ; Cheng, Weimin ; Han, Jie ; Feng, Ziyi ; Feng, Zhe ; Chen, GuoJian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-c6f007c10d9beab635a46ef98e0943ff589a4f373f98f00eb919ab8ec96f5da03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>AKT protein</topic><topic>Analysis</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Bone cancer</topic><topic>Cancer</topic><topic>Cancer therapies</topic><topic>Care and treatment</topic><topic>Cell proliferation</topic><topic>Cell survival</topic><topic>Chinese medicine</topic><topic>Drug dosages</topic><topic>Drug withdrawal</topic><topic>Gene expression</topic><topic>Immunohistochemistry</topic><topic>Lung cancer</topic><topic>Medical prognosis</topic><topic>Medicinal plants</topic><topic>Medicine, Botanic</topic><topic>Medicine, Herbal</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Oncology, Experimental</topic><topic>Pain</topic><topic>Pain perception</topic><topic>Pharmacy</topic><topic>Quality of life</topic><topic>Rapamycin</topic><topic>Ribosomal protein S6 kinase</topic><topic>RNA</topic><topic>Signal transduction</topic><topic>Small cell lung carcinoma</topic><topic>Tibia</topic><topic>TOR protein</topic><topic>Vascular endothelial growth factor</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Peng</creatorcontrib><creatorcontrib>Huang, Long</creatorcontrib><creatorcontrib>Cui, Zhenwei</creatorcontrib><creatorcontrib>Feng, Sitan</creatorcontrib><creatorcontrib>Feng, XinJian</creatorcontrib><creatorcontrib>Mo, 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Peng</au><au>Huang, Long</au><au>Cui, Zhenwei</au><au>Feng, Sitan</au><au>Feng, XinJian</au><au>Mo, Jian</au><au>Su, Bo</au><au>Cheng, Weimin</au><au>Han, Jie</au><au>Feng, Ziyi</au><au>Feng, Zhe</au><au>Chen, GuoJian</au><au>Makino, Toshiaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antinociceptive Effects of Shenling Baizhu through PI3K-Akt-mTOR Signaling Pathway in a Mouse Model of Bone Metastasis with Small-Cell Lung Cancer</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><date>2020</date><risdate>2020</risdate><volume>2020</volume><issue>2020</issue><spage>1</spage><epage>12</epage><pages>1-12</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Shenling Baizhu additive powder (SLBZ-AP), a formulation of a variety of natural medicinal plants, has clinical efficacy in treating cancers in previous studies. We explored the effect of SLBZ-AP in bone metastasis of lung cancer (BMLC) mice, and the possible mechanism involved was further investigated in the present study. Mice model of BMLC was made and treated with SLBZ-AP. Pain behavioral tests were performed to explore the effect on BMLC-induced pain in mice. TUNEL staining was used to investigate apoptosis. The mRNA expression of markers in the PI3K/Akt/mTOR pathway was measured by qPCR, and protein expression was detected by western blotting and immunohistochemistry analysis. SLBZ-AP relieved BMLC-induced pain and prolonged animals’ survival, promoted cell apoptosis in the marrow from the tibia of BMLC mice, and inhibited mRNA and protein expression of AKT, mTOR, P70S6, and VEGF, as well as protein expression of p-AKT, p-mTOR, p-P70S6, and VEGF upregulation in the marrow of tibia induced by BMLC, an effect which was similar to rapamycin. Our results suggested that SLBZ-AP may have antinociceptive effect and prolong survival of BMLC mice at least partially by inhibiting cell proliferation and promoting apoptosis through the PI3K/Akt/mTOR signaling pathway. SLBZ-AP may be a potential candidate for BMLC therapy.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>32655659</pmid><doi>10.1155/2020/4121483</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-0069-2564</orcidid><orcidid>https://orcid.org/0000-0003-1750-4400</orcidid><orcidid>https://orcid.org/0000-0001-5526-0238</orcidid><orcidid>https://orcid.org/0000-0003-3299-4567</orcidid><orcidid>https://orcid.org/0000-0002-2430-8312</orcidid><orcidid>https://orcid.org/0000-0001-8261-5559</orcidid><orcidid>https://orcid.org/0000-0002-7309-8083</orcidid><orcidid>https://orcid.org/0000-0002-4206-5385</orcidid><orcidid>https://orcid.org/0000-0001-5625-1501</orcidid><orcidid>https://orcid.org/0000-0002-9923-7774</orcidid><orcidid>https://orcid.org/0000-0002-1942-3105</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1741-427X |
| ispartof | Evidence-based complementary and alternative medicine, 2020, Vol.2020 (2020), p.1-12 |
| issn | 1741-427X 1741-4288 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7327581 |
| source | Wiley Online Library Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | 1-Phosphatidylinositol 3-kinase AKT protein Analysis Animals Apoptosis Bone cancer Cancer Cancer therapies Care and treatment Cell proliferation Cell survival Chinese medicine Drug dosages Drug withdrawal Gene expression Immunohistochemistry Lung cancer Medical prognosis Medicinal plants Medicine, Botanic Medicine, Herbal Metastases Metastasis Oncology, Experimental Pain Pain perception Pharmacy Quality of life Rapamycin Ribosomal protein S6 kinase RNA Signal transduction Small cell lung carcinoma Tibia TOR protein Vascular endothelial growth factor Western blotting |
| title | Antinociceptive Effects of Shenling Baizhu through PI3K-Akt-mTOR Signaling Pathway in a Mouse Model of Bone Metastasis with Small-Cell Lung Cancer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-18T23%3A05%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antinociceptive%20Effects%20of%20Shenling%20Baizhu%20through%20PI3K-Akt-mTOR%20Signaling%20Pathway%20in%20a%20Mouse%20Model%20of%20Bone%20Metastasis%20with%20Small-Cell%20Lung%20Cancer&rft.jtitle=Evidence-based%20complementary%20and%20alternative%20medicine&rft.au=Huang,%20Peng&rft.date=2020&rft.volume=2020&rft.issue=2020&rft.spage=1&rft.epage=12&rft.pages=1-12&rft.issn=1741-427X&rft.eissn=1741-4288&rft_id=info:doi/10.1155/2020/4121483&rft_dat=%3Cgale_pubme%3EA639548454%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2420064217&rft_id=info:pmid/32655659&rft_galeid=A639548454&rfr_iscdi=true |