In-vitro propagation, callus culture and bioactive lignan production in Phyllanthus tenellus Roxb: a new source of phyllanthin, hypophyllanthin and phyltetralin

This is the first report on identification and quantification of important hepatoprotective and anticancer polyphenolic lignans such as phyllanthin (PH), hypophyllanthin (HPH), niranthin (NH) and phyltetralin (PT) in natural plant and in vitro cultures of Phyllanthus tenellus Roxb. The identificatio...

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Veröffentlicht in:Scientific reports 2020-06, Vol.10 (1), p.10668-10668, Article 10668
Hauptverfasser: Nikule, Harichandra A., Nitnaware, Kirti M., Chambhare, Mahadev R., Kadam, Nitin S., Borde, Mahesh Y., Nikam, Tukaram D.
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container_title Scientific reports
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creator Nikule, Harichandra A.
Nitnaware, Kirti M.
Chambhare, Mahadev R.
Kadam, Nitin S.
Borde, Mahesh Y.
Nikam, Tukaram D.
description This is the first report on identification and quantification of important hepatoprotective and anticancer polyphenolic lignans such as phyllanthin (PH), hypophyllanthin (HPH), niranthin (NH) and phyltetralin (PT) in natural plant and in vitro cultures of Phyllanthus tenellus Roxb. The identification of lignans was carried out by Liquid Chromatography–High Resolution Mass Spectrometry (LC–HRMS) and quantified using High-Performance Liquid Chromatography (HPLC). In addition, an efficient protocol has been developed for multiple shoot induction in nodal explants of in vitro derived shoots of P. tenellus . Maximum number of shoot regeneration (7.83 ± 0.15) was achieved on medium incorporated with 1.0 mg/l 6-Benzylaminopurine (BAP). The medium containing Indole-3-acetic acid (IAA) 2 mg/l was superior for induction of rooting in in vitro raised shoots. The plantlets were acclimatized to the field condition with 100% survival. The quantitative HPLC analysis showed that the lignan content was variable with the auxins and cytokinins incorporated in the medium. The lignan content was higher in callus grown on Murashige and Skoog (MS) medium + 2.0 mg/l Naphthaleneacetic acid (NAA). The reported protocol can be used for mass propagation and application of biotechnological approaches for improvement of P. tenellus . The results indicate intriguing possibilities for the utilization of P. tenellus plant parts as an alternative source and of callus culture to scale up bioactive lignan production for pharmaceutical applications.
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subjects 631/449
631/449/1736
631/449/447
Acetic acid
Auxins
Benzyl Compounds - metabolism
Benzyladenine
Biotechnology
Callus
Callus culture
Chromatography
Culture Media - metabolism
Cytokinins
Cytokinins - metabolism
Explants
High-performance liquid chromatography
Humanities and Social Sciences
Indoleacetic acid
Indoleacetic Acids - metabolism
Lignans - metabolism
Liquid chromatography
Mass spectrometry
Mass spectroscopy
multidisciplinary
Naphthaleneacetic acid
Phyllanthus - metabolism
Phyllanthus tenellus
Plant propagation
Plant Roots - metabolism
Plant Shoots - metabolism
Plantlets
Propagation
Purines - metabolism
Rooting
Science
Science (multidisciplinary)
Shoots
title In-vitro propagation, callus culture and bioactive lignan production in Phyllanthus tenellus Roxb: a new source of phyllanthin, hypophyllanthin and phyltetralin
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