Cystine transporter expression is a marker to identify a subpopulation of canine adipose-derived stem cells
Adipose-derived stem cells (ADSCs) are promising cell sources for regenerative medicine due to the simplicity of their harvest and culture; however, their biological properties are not completely understood. Moreover, recent murine and human studies identified several functional subpopulations of AD...
Gespeichert in:
| Veröffentlicht in: | Journal of Veterinary Medical Science 2020, Vol.82(6), pp.713-720 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 720 |
|---|---|
| container_issue | 6 |
| container_start_page | 713 |
| container_title | Journal of Veterinary Medical Science |
| container_volume | 82 |
| creator | ITOH, Harumichi NISHIKAWA, Shimpei TANI, Kenji SUNAHARA, Hiroshi NAKAICHI, Munekazu ISERI, Toshie TAURA, Yasuho ITAMOTO, Kazuhito |
| description | Adipose-derived stem cells (ADSCs) are promising cell sources for regenerative medicine due to the simplicity of their harvest and culture; however, their biological properties are not completely understood. Moreover, recent murine and human studies identified several functional subpopulations of ADSCs varying in differentiation potential; however, there is a lack of research on canine ADSCs. Cystine transporter (xCT) is a stem cell marker in gastric and colon cancers that interacts with CD44 to enhance cystine uptake from the cell surface and subsequently accelerates intercellular glutathione levels. In this study, we identified a ~5% functional subpopulation of canine ADSCs with xCT+ expression (xCTHi). Compared with those of the xCT− subpopulation (xCTLo), the xCTHi subpopulation showed a significantly higher proliferation rate, higher expression of conventional stem cell markers (SOX2, KLF4, and c-Myc), and higher expression of adipogenic markers (FABP4 and PPARγ). By contrast, the xCTLo subpopulation showed significantly higher expression of osteogenic markers (BMP1 and SPP) than xCTHi cells. These results suggest xCT as a candidate marker for detecting a functional subpopulation of canine ADSCs. Mechanistically, xCT could increase the adipogenic potential while decreasing the osteogenic differentiation potential, which could serve as a valuable target marker in regenerative veterinary medicine. |
| doi_str_mv | 10.1292/jvms.19-0373 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7324832</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2419465502</sourcerecordid><originalsourceid>FETCH-LOGICAL-c618t-2ce8ccaf2d1428fd980fba8de4b6cd950c2325ddf24cc48814bdd37019d3d7053</originalsourceid><addsrcrecordid>eNpdkUtv1DAURi0EotPCjjWKxIZFU_xKYm-QyoiXVIkNrC3Hvmk9TeLg64yYf0-iGUbAxpZ8j47vp4-QV4zeMK75u91-wBumSyoa8YRsmJBN2Uihn5IN1awuG17RC3KJuKOUM1nr5-RCcF5rpusNedweMIcRipzsiFNMGVIBv6YEiCGORcDCFoNNj8tzjkXwMObQHZZHnNspTnNv88rFrnB2XEXWhykilB5S2IMvMMNQOOh7fEGedbZHeHm6r8iPTx-_b7-Ud98-f93e3pWuZiqX3IFyznbcM8lV57WiXWuVB9nWzuuKOi545X3HpXNSKSZb70VDmfbCN7QSV-T90TvN7QDeLSsn25sphSXIwUQbzL-TMTyY-7g3jeBSCb4I3p4EKf6cAbMZAq4R7AhxRsOFUpTSSq9_vfkP3cU5jUs8wyXTsq4qugqvj5RLETFBd16GUbO2aNYWDdNmbXHBX_8d4Az_qW0BPhyBHWZ7D2fAphxcD0eb4qZej5P1PHQPNhkYxW8h-LP1</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2419465502</pqid></control><display><type>article</type><title>Cystine transporter expression is a marker to identify a subpopulation of canine adipose-derived stem cells</title><source>PubMed (Medline)</source><source>J-STAGE Free</source><source>MEDLINE</source><source>EZB Electronic Journals Library</source><source>PubMed Central Open Access</source><creator>ITOH, Harumichi ; NISHIKAWA, Shimpei ; TANI, Kenji ; SUNAHARA, Hiroshi ; NAKAICHI, Munekazu ; ISERI, Toshie ; TAURA, Yasuho ; ITAMOTO, Kazuhito</creator><creatorcontrib>ITOH, Harumichi ; NISHIKAWA, Shimpei ; TANI, Kenji ; SUNAHARA, Hiroshi ; NAKAICHI, Munekazu ; ISERI, Toshie ; TAURA, Yasuho ; ITAMOTO, Kazuhito</creatorcontrib><description>Adipose-derived stem cells (ADSCs) are promising cell sources for regenerative medicine due to the simplicity of their harvest and culture; however, their biological properties are not completely understood. Moreover, recent murine and human studies identified several functional subpopulations of ADSCs varying in differentiation potential; however, there is a lack of research on canine ADSCs. Cystine transporter (xCT) is a stem cell marker in gastric and colon cancers that interacts with CD44 to enhance cystine uptake from the cell surface and subsequently accelerates intercellular glutathione levels. In this study, we identified a ~5% functional subpopulation of canine ADSCs with xCT+ expression (xCTHi). Compared with those of the xCT− subpopulation (xCTLo), the xCTHi subpopulation showed a significantly higher proliferation rate, higher expression of conventional stem cell markers (SOX2, KLF4, and c-Myc), and higher expression of adipogenic markers (FABP4 and PPARγ). By contrast, the xCTLo subpopulation showed significantly higher expression of osteogenic markers (BMP1 and SPP) than xCTHi cells. These results suggest xCT as a candidate marker for detecting a functional subpopulation of canine ADSCs. Mechanistically, xCT could increase the adipogenic potential while decreasing the osteogenic differentiation potential, which could serve as a valuable target marker in regenerative veterinary medicine.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.19-0373</identifier><identifier>PMID: 32269196</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Adipogenesis ; adipogenic differentiation ; Adipose Tissue - cytology ; adipose-derived stem cell ; Animals ; Antigens, Differentiation - metabolism ; c-Myc protein ; CD44 antigen ; Cell culture ; Cell Proliferation ; Cell Separation ; Cell surface ; Colon ; Cystine - metabolism ; cystine transporter ; Dogs - anatomy & histology ; flow cytometry ; Glutathione ; KLF4 protein ; Mesenchymal Stem Cells - metabolism ; Myc protein ; Osteogenesis ; osteogenic differentiation ; Regenerative medicine ; Stem cell transplantation ; Stem cells ; Surgery ; Veterinary medicine</subject><ispartof>Journal of Veterinary Medical Science, 2020, Vol.82(6), pp.713-720</ispartof><rights>2020 by the Japanese Society of Veterinary Science</rights><rights>2020. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 The Japanese Society of Veterinary Science 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c618t-2ce8ccaf2d1428fd980fba8de4b6cd950c2325ddf24cc48814bdd37019d3d7053</citedby><cites>FETCH-LOGICAL-c618t-2ce8ccaf2d1428fd980fba8de4b6cd950c2325ddf24cc48814bdd37019d3d7053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324832/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324832/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32269196$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ITOH, Harumichi</creatorcontrib><creatorcontrib>NISHIKAWA, Shimpei</creatorcontrib><creatorcontrib>TANI, Kenji</creatorcontrib><creatorcontrib>SUNAHARA, Hiroshi</creatorcontrib><creatorcontrib>NAKAICHI, Munekazu</creatorcontrib><creatorcontrib>ISERI, Toshie</creatorcontrib><creatorcontrib>TAURA, Yasuho</creatorcontrib><creatorcontrib>ITAMOTO, Kazuhito</creatorcontrib><title>Cystine transporter expression is a marker to identify a subpopulation of canine adipose-derived stem cells</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Adipose-derived stem cells (ADSCs) are promising cell sources for regenerative medicine due to the simplicity of their harvest and culture; however, their biological properties are not completely understood. Moreover, recent murine and human studies identified several functional subpopulations of ADSCs varying in differentiation potential; however, there is a lack of research on canine ADSCs. Cystine transporter (xCT) is a stem cell marker in gastric and colon cancers that interacts with CD44 to enhance cystine uptake from the cell surface and subsequently accelerates intercellular glutathione levels. In this study, we identified a ~5% functional subpopulation of canine ADSCs with xCT+ expression (xCTHi). Compared with those of the xCT− subpopulation (xCTLo), the xCTHi subpopulation showed a significantly higher proliferation rate, higher expression of conventional stem cell markers (SOX2, KLF4, and c-Myc), and higher expression of adipogenic markers (FABP4 and PPARγ). By contrast, the xCTLo subpopulation showed significantly higher expression of osteogenic markers (BMP1 and SPP) than xCTHi cells. These results suggest xCT as a candidate marker for detecting a functional subpopulation of canine ADSCs. Mechanistically, xCT could increase the adipogenic potential while decreasing the osteogenic differentiation potential, which could serve as a valuable target marker in regenerative veterinary medicine.</description><subject>Adipogenesis</subject><subject>adipogenic differentiation</subject><subject>Adipose Tissue - cytology</subject><subject>adipose-derived stem cell</subject><subject>Animals</subject><subject>Antigens, Differentiation - metabolism</subject><subject>c-Myc protein</subject><subject>CD44 antigen</subject><subject>Cell culture</subject><subject>Cell Proliferation</subject><subject>Cell Separation</subject><subject>Cell surface</subject><subject>Colon</subject><subject>Cystine - metabolism</subject><subject>cystine transporter</subject><subject>Dogs - anatomy & histology</subject><subject>flow cytometry</subject><subject>Glutathione</subject><subject>KLF4 protein</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>Myc protein</subject><subject>Osteogenesis</subject><subject>osteogenic differentiation</subject><subject>Regenerative medicine</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Surgery</subject><subject>Veterinary medicine</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtv1DAURi0EotPCjjWKxIZFU_xKYm-QyoiXVIkNrC3Hvmk9TeLg64yYf0-iGUbAxpZ8j47vp4-QV4zeMK75u91-wBumSyoa8YRsmJBN2Uihn5IN1awuG17RC3KJuKOUM1nr5-RCcF5rpusNedweMIcRipzsiFNMGVIBv6YEiCGORcDCFoNNj8tzjkXwMObQHZZHnNspTnNv88rFrnB2XEXWhykilB5S2IMvMMNQOOh7fEGedbZHeHm6r8iPTx-_b7-Ud98-f93e3pWuZiqX3IFyznbcM8lV57WiXWuVB9nWzuuKOi545X3HpXNSKSZb70VDmfbCN7QSV-T90TvN7QDeLSsn25sphSXIwUQbzL-TMTyY-7g3jeBSCb4I3p4EKf6cAbMZAq4R7AhxRsOFUpTSSq9_vfkP3cU5jUs8wyXTsq4qugqvj5RLETFBd16GUbO2aNYWDdNmbXHBX_8d4Az_qW0BPhyBHWZ7D2fAphxcD0eb4qZej5P1PHQPNhkYxW8h-LP1</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>ITOH, Harumichi</creator><creator>NISHIKAWA, Shimpei</creator><creator>TANI, Kenji</creator><creator>SUNAHARA, Hiroshi</creator><creator>NAKAICHI, Munekazu</creator><creator>ISERI, Toshie</creator><creator>TAURA, Yasuho</creator><creator>ITAMOTO, Kazuhito</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2020</creationdate><title>Cystine transporter expression is a marker to identify a subpopulation of canine adipose-derived stem cells</title><author>ITOH, Harumichi ; NISHIKAWA, Shimpei ; TANI, Kenji ; SUNAHARA, Hiroshi ; NAKAICHI, Munekazu ; ISERI, Toshie ; TAURA, Yasuho ; ITAMOTO, Kazuhito</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c618t-2ce8ccaf2d1428fd980fba8de4b6cd950c2325ddf24cc48814bdd37019d3d7053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adipogenesis</topic><topic>adipogenic differentiation</topic><topic>Adipose Tissue - cytology</topic><topic>adipose-derived stem cell</topic><topic>Animals</topic><topic>Antigens, Differentiation - metabolism</topic><topic>c-Myc protein</topic><topic>CD44 antigen</topic><topic>Cell culture</topic><topic>Cell Proliferation</topic><topic>Cell Separation</topic><topic>Cell surface</topic><topic>Colon</topic><topic>Cystine - metabolism</topic><topic>cystine transporter</topic><topic>Dogs - anatomy & histology</topic><topic>flow cytometry</topic><topic>Glutathione</topic><topic>KLF4 protein</topic><topic>Mesenchymal Stem Cells - metabolism</topic><topic>Myc protein</topic><topic>Osteogenesis</topic><topic>osteogenic differentiation</topic><topic>Regenerative medicine</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Surgery</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ITOH, Harumichi</creatorcontrib><creatorcontrib>NISHIKAWA, Shimpei</creatorcontrib><creatorcontrib>TANI, Kenji</creatorcontrib><creatorcontrib>SUNAHARA, Hiroshi</creatorcontrib><creatorcontrib>NAKAICHI, Munekazu</creatorcontrib><creatorcontrib>ISERI, Toshie</creatorcontrib><creatorcontrib>TAURA, Yasuho</creatorcontrib><creatorcontrib>ITAMOTO, Kazuhito</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ITOH, Harumichi</au><au>NISHIKAWA, Shimpei</au><au>TANI, Kenji</au><au>SUNAHARA, Hiroshi</au><au>NAKAICHI, Munekazu</au><au>ISERI, Toshie</au><au>TAURA, Yasuho</au><au>ITAMOTO, Kazuhito</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cystine transporter expression is a marker to identify a subpopulation of canine adipose-derived stem cells</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2020</date><risdate>2020</risdate><volume>82</volume><issue>6</issue><spage>713</spage><epage>720</epage><pages>713-720</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Adipose-derived stem cells (ADSCs) are promising cell sources for regenerative medicine due to the simplicity of their harvest and culture; however, their biological properties are not completely understood. Moreover, recent murine and human studies identified several functional subpopulations of ADSCs varying in differentiation potential; however, there is a lack of research on canine ADSCs. Cystine transporter (xCT) is a stem cell marker in gastric and colon cancers that interacts with CD44 to enhance cystine uptake from the cell surface and subsequently accelerates intercellular glutathione levels. In this study, we identified a ~5% functional subpopulation of canine ADSCs with xCT+ expression (xCTHi). Compared with those of the xCT− subpopulation (xCTLo), the xCTHi subpopulation showed a significantly higher proliferation rate, higher expression of conventional stem cell markers (SOX2, KLF4, and c-Myc), and higher expression of adipogenic markers (FABP4 and PPARγ). By contrast, the xCTLo subpopulation showed significantly higher expression of osteogenic markers (BMP1 and SPP) than xCTHi cells. These results suggest xCT as a candidate marker for detecting a functional subpopulation of canine ADSCs. Mechanistically, xCT could increase the adipogenic potential while decreasing the osteogenic differentiation potential, which could serve as a valuable target marker in regenerative veterinary medicine.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>32269196</pmid><doi>10.1292/jvms.19-0373</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-7250 |
| ispartof | Journal of Veterinary Medical Science, 2020, Vol.82(6), pp.713-720 |
| issn | 0916-7250 1347-7439 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7324832 |
| source | PubMed (Medline); J-STAGE Free; MEDLINE; EZB Electronic Journals Library; PubMed Central Open Access |
| subjects | Adipogenesis adipogenic differentiation Adipose Tissue - cytology adipose-derived stem cell Animals Antigens, Differentiation - metabolism c-Myc protein CD44 antigen Cell culture Cell Proliferation Cell Separation Cell surface Colon Cystine - metabolism cystine transporter Dogs - anatomy & histology flow cytometry Glutathione KLF4 protein Mesenchymal Stem Cells - metabolism Myc protein Osteogenesis osteogenic differentiation Regenerative medicine Stem cell transplantation Stem cells Surgery Veterinary medicine |
| title | Cystine transporter expression is a marker to identify a subpopulation of canine adipose-derived stem cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T17%3A30%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cystine%20transporter%20expression%20is%20a%20marker%20to%20identify%20a%20subpopulation%20of%20canine%20adipose-derived%20stem%20cells&rft.jtitle=Journal%20of%20Veterinary%20Medical%20Science&rft.au=ITOH,%20Harumichi&rft.date=2020&rft.volume=82&rft.issue=6&rft.spage=713&rft.epage=720&rft.pages=713-720&rft.issn=0916-7250&rft.eissn=1347-7439&rft_id=info:doi/10.1292/jvms.19-0373&rft_dat=%3Cproquest_pubme%3E2419465502%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2419465502&rft_id=info:pmid/32269196&rfr_iscdi=true |