Molecular characterization of Brucella ceti from a bottlenose dolphin (Tursiops truncatus) with osteomyelitis in the western Pacific
Although the presence of Brucella spp. in the western Pacific has been suggested by epidemiological studies on cetaceans, it has not been confirmed by bacterial isolation. Here, for the first time, we report that a marine Brucella strain was isolated in the western Pacific from a bottlenose dolphin...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2020, Vol.82(6), pp.754-758 |
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| creator | UENO, Yuichi YANAGISAWA, Makio KINO, Sayuri SHIGENO, Satoru OSAKI, Makoto TAKAMATSU, Daisuke KATSUDA, Ken MARUYAMA, Tadashi OHISHI, Kazue |
| description | Although the presence of Brucella spp. in the western Pacific has been suggested by epidemiological studies on cetaceans, it has not been confirmed by bacterial isolation. Here, for the first time, we report that a marine Brucella strain was isolated in the western Pacific from a bottlenose dolphin with osteomyelitis. The isolate from the lesion was confirmed to be B. ceti of sequence type 27 by multilocus sequence typing and Bruce-ladder PCR. Infrequent-restriction-site PCR and omp2 gene sequencing revealed that molecular characteristics of this isolate were similar to those of Brucella DNA previously detected from minke whales in the western North Pacific. These results suggest that genetically related Brucella strains circulate in cetacean species in this region. |
| doi_str_mv | 10.1292/jvms.20-0015 |
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Here, for the first time, we report that a marine Brucella strain was isolated in the western Pacific from a bottlenose dolphin with osteomyelitis. The isolate from the lesion was confirmed to be B. ceti of sequence type 27 by multilocus sequence typing and Bruce-ladder PCR. Infrequent-restriction-site PCR and omp2 gene sequencing revealed that molecular characteristics of this isolate were similar to those of Brucella DNA previously detected from minke whales in the western North Pacific. These results suggest that genetically related Brucella strains circulate in cetacean species in this region.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.20-0015</identifier><identifier>PMID: 32336742</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Animals ; Bacteriology ; Bottle-Nosed Dolphin - microbiology ; bottlenose dolphin ; Brucella ; Brucella - genetics ; Brucella - isolation & purification ; Brucella ceti ; brucellosis ; Brucellosis - diagnosis ; Brucellosis - microbiology ; Brucellosis - veterinary ; DNA, Bacterial ; Dolphins & porpoises ; Epidemiology ; Male ; molecular characterization ; Multilocus Sequence Typing ; Osteomyelitis ; Osteomyelitis - microbiology ; Osteomyelitis - veterinary ; Outer membrane proteins ; Pacific Ocean - epidemiology ; Polymerase Chain Reaction ; Tursiops truncatus ; western Pacific</subject><ispartof>Journal of Veterinary Medical Science, 2020, Vol.82(6), pp.754-758</ispartof><rights>2020 by the Japanese Society of Veterinary Science</rights><rights>2020. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja (the “License”). 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Vet. Med. Sci.</addtitle><description>Although the presence of Brucella spp. in the western Pacific has been suggested by epidemiological studies on cetaceans, it has not been confirmed by bacterial isolation. Here, for the first time, we report that a marine Brucella strain was isolated in the western Pacific from a bottlenose dolphin with osteomyelitis. The isolate from the lesion was confirmed to be B. ceti of sequence type 27 by multilocus sequence typing and Bruce-ladder PCR. Infrequent-restriction-site PCR and omp2 gene sequencing revealed that molecular characteristics of this isolate were similar to those of Brucella DNA previously detected from minke whales in the western North Pacific. These results suggest that genetically related Brucella strains circulate in cetacean species in this region.</description><subject>Animals</subject><subject>Bacteriology</subject><subject>Bottle-Nosed Dolphin - microbiology</subject><subject>bottlenose dolphin</subject><subject>Brucella</subject><subject>Brucella - genetics</subject><subject>Brucella - isolation & purification</subject><subject>Brucella ceti</subject><subject>brucellosis</subject><subject>Brucellosis - diagnosis</subject><subject>Brucellosis - microbiology</subject><subject>Brucellosis - veterinary</subject><subject>DNA, Bacterial</subject><subject>Dolphins & porpoises</subject><subject>Epidemiology</subject><subject>Male</subject><subject>molecular characterization</subject><subject>Multilocus Sequence Typing</subject><subject>Osteomyelitis</subject><subject>Osteomyelitis - microbiology</subject><subject>Osteomyelitis - veterinary</subject><subject>Outer membrane proteins</subject><subject>Pacific Ocean - epidemiology</subject><subject>Polymerase Chain Reaction</subject><subject>Tursiops truncatus</subject><subject>western Pacific</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdks9rFDEcxYNY7Fq9eZaAlwpOzY_J_LgUbKm1UNFDPYdM5judLJlkTTIt9dw_3Iy7XVQCCeR9eMnLC0JvKDmhrGUf13dTPGGkIISKZ2hFeVkXdcnb52hFWloVNRPkEL2McU0Io2XVvkCHnHFe1SVbocev3oKerQpYjyoonSCYXyoZ77Af8FmYNVirsIZk8BD8hBXufEoWnI-Ae283o3H4-GYO0fhNxCnMTqs0x_f43qQR-5jATw9gTTIRZzSNgO8h7waHvyttBqNfoYNB2Qivd-sR-vH54ub8S3H97fLq_NN1oQWvUyG0IqqGulW0ESD6pu_5QGhORTpQvS67XlREd1XVlD0TvCMto7TJkhB1P3B-hE63vpu5m6DX4FJQVm6CmVR4kF4Z-a_izChv_Z2sOSsb2maD451B8D_nHEJOJv55IAd-jpLxVrCKMLKc9e4_dO3n4HI8yUralpXII1MftpQOPsYAw_4ylMilXrnUKxmRS70Zf_t3gD381GcGzrbAOiZ1C3tAhWS0ha1bw2S1TDvXvbh8AAmO_waxELy3</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>UENO, Yuichi</creator><creator>YANAGISAWA, Makio</creator><creator>KINO, Sayuri</creator><creator>SHIGENO, Satoru</creator><creator>OSAKI, Makoto</creator><creator>TAKAMATSU, Daisuke</creator><creator>KATSUDA, Ken</creator><creator>MARUYAMA, Tadashi</creator><creator>OHISHI, Kazue</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200601</creationdate><title>Molecular characterization of Brucella ceti from a bottlenose dolphin (Tursiops truncatus) with osteomyelitis in the western Pacific</title><author>UENO, Yuichi ; 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Vet. Med. Sci.</addtitle><date>2020-06-01</date><risdate>2020</risdate><volume>82</volume><issue>6</issue><spage>754</spage><epage>758</epage><pages>754-758</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Although the presence of Brucella spp. in the western Pacific has been suggested by epidemiological studies on cetaceans, it has not been confirmed by bacterial isolation. Here, for the first time, we report that a marine Brucella strain was isolated in the western Pacific from a bottlenose dolphin with osteomyelitis. The isolate from the lesion was confirmed to be B. ceti of sequence type 27 by multilocus sequence typing and Bruce-ladder PCR. Infrequent-restriction-site PCR and omp2 gene sequencing revealed that molecular characteristics of this isolate were similar to those of Brucella DNA previously detected from minke whales in the western North Pacific. 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| subjects | Animals Bacteriology Bottle-Nosed Dolphin - microbiology bottlenose dolphin Brucella Brucella - genetics Brucella - isolation & purification Brucella ceti brucellosis Brucellosis - diagnosis Brucellosis - microbiology Brucellosis - veterinary DNA, Bacterial Dolphins & porpoises Epidemiology Male molecular characterization Multilocus Sequence Typing Osteomyelitis Osteomyelitis - microbiology Osteomyelitis - veterinary Outer membrane proteins Pacific Ocean - epidemiology Polymerase Chain Reaction Tursiops truncatus western Pacific |
| title | Molecular characterization of Brucella ceti from a bottlenose dolphin (Tursiops truncatus) with osteomyelitis in the western Pacific |
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