Chemical Evaluation of the Effects of Storage Conditions on the Botanical Goldenseal using Marker-based and Metabolomics Approaches
commonly known as goldenseal, is a botanical native to the southeastern United States that has been used for the treatment of infection. The activity of goldenseal is often attributed to the presence of alkaloids (cyclic, nitrogen-containing compounds) present within its roots. Chemical components o...
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| Veröffentlicht in: | The Yale journal of biology & medicine 2020-06, Vol.93 (2), p.265-275 |
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| description | commonly known as goldenseal, is a botanical native to the southeastern United States that has been used for the treatment of infection. The activity of goldenseal is often attributed to the presence of alkaloids (cyclic, nitrogen-containing compounds) present within its roots. Chemical components of botanical supplements like goldenseal may face degradation if not stored properly. The purpose of the research was to analyze the stability of known and unknown metabolites of
during exposure to different storage conditions using mass spectrometry. Three abundant metabolites of
, berberine, canadine, and hydrastine, were chosen for targeted analysis, and the stability of unknown metabolites was evaluated using untargeted metabolomics. The analysis and evaluation of
samples were performed utilizing LC-MS and Principal Component Analysis (PCA). The research project focused on identifying the chemical changes in the metabolite content of
under different temperature conditions (40°C ± 5°C, 20°C ± 5°C , and 4°C ± 5°C), different light:dark (hr:hr) cycles (16:8, 12:12, and 0:24), and different sample conditions (powdered roots versus whole roots) over a six month period. The results of this 6-month study revealed that the storage conditions evaluated had no significant effects on the chemical composition of
roots. Hence, as long as
roots are stored within the storage conditions tested in the study, no significant changes in chemical compositions of metabolites are expected. |
| format | Article |
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during exposure to different storage conditions using mass spectrometry. Three abundant metabolites of
, berberine, canadine, and hydrastine, were chosen for targeted analysis, and the stability of unknown metabolites was evaluated using untargeted metabolomics. The analysis and evaluation of
samples were performed utilizing LC-MS and Principal Component Analysis (PCA). The research project focused on identifying the chemical changes in the metabolite content of
under different temperature conditions (40°C ± 5°C, 20°C ± 5°C , and 4°C ± 5°C), different light:dark (hr:hr) cycles (16:8, 12:12, and 0:24), and different sample conditions (powdered roots versus whole roots) over a six month period. The results of this 6-month study revealed that the storage conditions evaluated had no significant effects on the chemical composition of
roots. Hence, as long as
roots are stored within the storage conditions tested in the study, no significant changes in chemical compositions of metabolites are expected.</description><identifier>ISSN: 0044-0086</identifier><identifier>EISSN: 1551-4056</identifier><identifier>PMID: 32607087</identifier><language>eng</language><publisher>United States: Yale Journal of Biology and Medicine</publisher><subject>Benzylisoquinolines - analysis ; Berberine ; Berberine - analogs & derivatives ; Berberine - analysis ; Berberine Alkaloids - analysis ; Berberine Alkaloids - pharmacology ; Dietary supplements ; Drug Stability ; Drug Storage - methods ; Drug Storage - standards ; Gas flow ; Herbal medicine ; High temperature ; Humans ; Hydrastis ; Hydrastis canadensis ; Infections - drug therapy ; Mass spectrometry ; Mass Spectrometry - methods ; Mass spectroscopy ; Metabolites ; Metabolomics ; Original Contribution ; Plant Preparations - chemistry ; Plant Preparations - pharmacology ; Plant Roots - chemistry ; Principal Component Analysis - methods ; Principal components analysis ; Retention ; Roots ; Scientific imaging ; Software ; Storage conditions</subject><ispartof>The Yale journal of biology & medicine, 2020-06, Vol.93 (2), p.265-275</ispartof><rights>Copyright ©2020, Yale Journal of Biology and Medicine.</rights><rights>2020. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Sourced from the United States National Library of Medicine® (NLM). This work may not reflect the most current or accurate data available from NLM.</rights><rights>Copyright ©2020, Yale Journal of Biology and Medicine 2020 Yale Journal of Biology and Medicine</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309669/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309669/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32607087$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khin, Manead</creatorcontrib><creatorcontrib>Cech, Nadja B</creatorcontrib><creatorcontrib>Kellogg, Joshua J</creatorcontrib><creatorcontrib>Caesar, Lindsay K</creatorcontrib><title>Chemical Evaluation of the Effects of Storage Conditions on the Botanical Goldenseal using Marker-based and Metabolomics Approaches</title><title>The Yale journal of biology & medicine</title><addtitle>Yale J Biol Med</addtitle><description>commonly known as goldenseal, is a botanical native to the southeastern United States that has been used for the treatment of infection. The activity of goldenseal is often attributed to the presence of alkaloids (cyclic, nitrogen-containing compounds) present within its roots. Chemical components of botanical supplements like goldenseal may face degradation if not stored properly. The purpose of the research was to analyze the stability of known and unknown metabolites of
during exposure to different storage conditions using mass spectrometry. Three abundant metabolites of
, berberine, canadine, and hydrastine, were chosen for targeted analysis, and the stability of unknown metabolites was evaluated using untargeted metabolomics. The analysis and evaluation of
samples were performed utilizing LC-MS and Principal Component Analysis (PCA). The research project focused on identifying the chemical changes in the metabolite content of
under different temperature conditions (40°C ± 5°C, 20°C ± 5°C , and 4°C ± 5°C), different light:dark (hr:hr) cycles (16:8, 12:12, and 0:24), and different sample conditions (powdered roots versus whole roots) over a six month period. The results of this 6-month study revealed that the storage conditions evaluated had no significant effects on the chemical composition of
roots. Hence, as long as
roots are stored within the storage conditions tested in the study, no significant changes in chemical compositions of metabolites are expected.</description><subject>Benzylisoquinolines - analysis</subject><subject>Berberine</subject><subject>Berberine - analogs & derivatives</subject><subject>Berberine - analysis</subject><subject>Berberine Alkaloids - analysis</subject><subject>Berberine Alkaloids - pharmacology</subject><subject>Dietary supplements</subject><subject>Drug Stability</subject><subject>Drug Storage - methods</subject><subject>Drug Storage - standards</subject><subject>Gas flow</subject><subject>Herbal medicine</subject><subject>High temperature</subject><subject>Humans</subject><subject>Hydrastis</subject><subject>Hydrastis canadensis</subject><subject>Infections - drug therapy</subject><subject>Mass spectrometry</subject><subject>Mass Spectrometry - methods</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Original Contribution</subject><subject>Plant Preparations - chemistry</subject><subject>Plant Preparations - pharmacology</subject><subject>Plant Roots - chemistry</subject><subject>Principal Component Analysis - methods</subject><subject>Principal components analysis</subject><subject>Retention</subject><subject>Roots</subject><subject>Scientific imaging</subject><subject>Software</subject><subject>Storage conditions</subject><issn>0044-0086</issn><issn>1551-4056</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkUtLxDAQgIsouj7-ggS8eCmkadI0F0GX9QErHtRzmTaT3a7dpCap4Nk_btcX6inz-Phmhmwlk0yILOVUFNvJhFLOU0rLYi_ZD2FFaS4yke8mezkrqKSlnCRv0yWu2wY6MnuBboDYOkucIXGJZGYMNjFs0vvoPCyQTJ3V7YYZq_YDunAR7IfgynUabcAxHEJrF-QW_BP6tIaAmoDV5BYj1K5z48BAzvveO2iWGA6THQNdwKOv9yB5vJw9TK_T-d3VzfR8nvZM8ZiWuhaaUyaMzHMQrNC1kqiVKRvORC244UwrZURhZIO8wRozWtQ6k6CUUDo_SM4-vf1Qr1E3aKOHrup9uwb_Wjloq78d2y6rhXupZE5VUahRcPol8O55wBCrdRsa7Dqw6IZQMZ4pnlHJN-jJP3TlBm_H8yomGR9lsuQjdfx7o59Vvv8nfwdN-4_E</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>Khin, Manead</creator><creator>Cech, Nadja B</creator><creator>Kellogg, Joshua J</creator><creator>Caesar, Lindsay K</creator><general>Yale Journal of Biology and Medicine</general><general>YJBM</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200601</creationdate><title>Chemical Evaluation of the Effects of Storage Conditions on the Botanical Goldenseal using Marker-based and Metabolomics Approaches</title><author>Khin, Manead ; Cech, Nadja B ; Kellogg, Joshua J ; Caesar, Lindsay K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p294t-8db5d4025f733a526db97ed9f8c425b54f42d99f56f7ce4cebe106bd17a9959d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Benzylisoquinolines - analysis</topic><topic>Berberine</topic><topic>Berberine - analogs & derivatives</topic><topic>Berberine - analysis</topic><topic>Berberine Alkaloids - analysis</topic><topic>Berberine Alkaloids - pharmacology</topic><topic>Dietary supplements</topic><topic>Drug Stability</topic><topic>Drug Storage - methods</topic><topic>Drug Storage - standards</topic><topic>Gas flow</topic><topic>Herbal medicine</topic><topic>High temperature</topic><topic>Humans</topic><topic>Hydrastis</topic><topic>Hydrastis canadensis</topic><topic>Infections - drug therapy</topic><topic>Mass spectrometry</topic><topic>Mass Spectrometry - methods</topic><topic>Mass spectroscopy</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Original Contribution</topic><topic>Plant Preparations - chemistry</topic><topic>Plant Preparations - pharmacology</topic><topic>Plant Roots - chemistry</topic><topic>Principal Component Analysis - methods</topic><topic>Principal components analysis</topic><topic>Retention</topic><topic>Roots</topic><topic>Scientific imaging</topic><topic>Software</topic><topic>Storage conditions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khin, Manead</creatorcontrib><creatorcontrib>Cech, Nadja B</creatorcontrib><creatorcontrib>Kellogg, Joshua J</creatorcontrib><creatorcontrib>Caesar, Lindsay K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Yale journal of biology & medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khin, Manead</au><au>Cech, Nadja B</au><au>Kellogg, Joshua J</au><au>Caesar, Lindsay K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemical Evaluation of the Effects of Storage Conditions on the Botanical Goldenseal using Marker-based and Metabolomics Approaches</atitle><jtitle>The Yale journal of biology & medicine</jtitle><addtitle>Yale J Biol Med</addtitle><date>2020-06-01</date><risdate>2020</risdate><volume>93</volume><issue>2</issue><spage>265</spage><epage>275</epage><pages>265-275</pages><issn>0044-0086</issn><eissn>1551-4056</eissn><abstract>commonly known as goldenseal, is a botanical native to the southeastern United States that has been used for the treatment of infection. The activity of goldenseal is often attributed to the presence of alkaloids (cyclic, nitrogen-containing compounds) present within its roots. Chemical components of botanical supplements like goldenseal may face degradation if not stored properly. The purpose of the research was to analyze the stability of known and unknown metabolites of
during exposure to different storage conditions using mass spectrometry. Three abundant metabolites of
, berberine, canadine, and hydrastine, were chosen for targeted analysis, and the stability of unknown metabolites was evaluated using untargeted metabolomics. The analysis and evaluation of
samples were performed utilizing LC-MS and Principal Component Analysis (PCA). The research project focused on identifying the chemical changes in the metabolite content of
under different temperature conditions (40°C ± 5°C, 20°C ± 5°C , and 4°C ± 5°C), different light:dark (hr:hr) cycles (16:8, 12:12, and 0:24), and different sample conditions (powdered roots versus whole roots) over a six month period. The results of this 6-month study revealed that the storage conditions evaluated had no significant effects on the chemical composition of
roots. Hence, as long as
roots are stored within the storage conditions tested in the study, no significant changes in chemical compositions of metabolites are expected.</abstract><cop>United States</cop><pub>Yale Journal of Biology and Medicine</pub><pmid>32607087</pmid><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Benzylisoquinolines - analysis Berberine Berberine - analogs & derivatives Berberine - analysis Berberine Alkaloids - analysis Berberine Alkaloids - pharmacology Dietary supplements Drug Stability Drug Storage - methods Drug Storage - standards Gas flow Herbal medicine High temperature Humans Hydrastis Hydrastis canadensis Infections - drug therapy Mass spectrometry Mass Spectrometry - methods Mass spectroscopy Metabolites Metabolomics Original Contribution Plant Preparations - chemistry Plant Preparations - pharmacology Plant Roots - chemistry Principal Component Analysis - methods Principal components analysis Retention Roots Scientific imaging Software Storage conditions |
| title | Chemical Evaluation of the Effects of Storage Conditions on the Botanical Goldenseal using Marker-based and Metabolomics Approaches |
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