High-Throughput Screening for Substrate Specificity-Adapted Mutants of the Nisin Dehydratase NisB

Microbial lanthipeptides are formed by a two-step enzymatic introduction of (methyl)lanthionine rings. A dehydratase catalyzes the dehydration of serine and threonine residues, yielding dehydroalanine and dehydrobutyrine, respectively. Cyclase-catalyzed coupling of the formed dehydroresidues to cyst...

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Veröffentlicht in:	ACS synthetic biology 2020-06, Vol.9 (6), p.1468-1478
Hauptverfasser:	Zhao, Xinghong, Cebrián, Rubén, Fu, Yuxin, Rink, Rick, Bosma, Tjibbe, Moll, Gert N, Kuipers, Oscar P
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine - analogs & derivatives Alanine - chemistry Amino Acid Sequence Bacterial Proteins - genetics Bacterial Proteins - metabolism High-Throughput Screening Assays - methods Lactococcus lactis - enzymology Ligands Membrane Proteins - genetics Membrane Proteins - metabolism Mutagenesis, Site-Directed Peptides - chemistry Peptides - metabolism Protein Binding Streptavidin - chemistry Streptavidin - metabolism Substrate Specificity Sulfides - chemistry
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