CRISPR off-target detection with DISCOVER-seq

DISCOVER-seq (discovery of in situ Cas off-targets and verification by sequencing) is a broadly applicable approach for unbiased CRISPR–Cas off-target identification in cells and tissues. It leverages the recruitment of DNA repair factors to double-strand breaks (DSBs) after genome editing with CRIS...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nature protocols 2020-05, Vol.15 (5), p.1775-1799
Hauptverfasser:	Wienert, Beeke, Wyman, Stacia K., Yeh, Charles D., Conklin, Bruce R., Corn, Jacob E.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2217/2088 631/337/1427/2122 631/337/4041/3196 631/61/201/2110 Analysis Analytical Chemistry Animal models Animals Bioinformatics Biological Techniques Biomedical and Life Sciences Cell culture Chromatin Clustered Regularly Interspaced Short Palindromic Repeats Computational Biology/Bioinformatics CRISPR CRISPR-Cas Systems Deoxyribonucleic acid DNA DNA binding proteins DNA Breaks, Double-Stranded DNA Repair DNA sequencing Editing Gene Editing - methods Gene sequencing Genome editing Genomes Genomics Humans Immunoprecipitation K562 Cells Life Sciences Methods Mice Microarrays MRE11 protein Next-generation sequencing Nuclease Nucleases Nucleotide sequencing Organic Chemistry Protocol Recruitment Repair Sequence Analysis, DNA Target detection Target recognition
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!