Promising abscopal effect of combination therapy with thermal tumour ablation and intratumoural OK-432 injection in the rat osteosarcoma model
Treatment options for metastatic osteosarcoma are limited. The present study aimed to evaluate whether radiofrequency ablation (RFA) combined with intratumoural OK-432 injection induces systemic anti-tumour immunity in rat osteosarcoma model. Eighty of 145 rats were assigned to four groups to evalua...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2020-06, Vol.10 (1), p.9679-9679, Article 9679 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 9679 |
|---|---|
| container_issue | 1 |
| container_start_page | 9679 |
| container_title | Scientific reports |
| container_volume | 10 |
| creator | Iwai, Tadashi Oebisu, Naoto Hoshi, Manabu Orita, Kumi Yamamoto, Akira Hamamoto, Shinichi Kageyama, Ken Nakamura, Hiroaki |
| description | Treatment options for metastatic osteosarcoma are limited. The present study aimed to evaluate whether radiofrequency ablation (RFA) combined with intratumoural OK-432 injection induces systemic anti-tumour immunity in rat osteosarcoma model. Eighty of 145 rats were assigned to four groups to evaluate overall survival and tumour size: control (no treatment), RFA-only, OK-432, and RFA-OK-432. The remaining 65 were assigned for histological examination. Maximum diameters of tibial and lung tumours were determined. Tumour samples were histologically examined using haematoxylin-eosin and immunohistochemical staining. Overall survival was significantly prolonged in the RFA-OK-432 group compared to the RFA-only and OK-432 groups. Only rats in the RFA-OK-432 group exhibited significant decreases in maximum tumour diameter after treatment. Ki-67-positive tumour cells in the RFA-OK-432 group were significantly stained negative on immunohistochemical analysis as opposed to those in the RFA-only and OK-432 groups. The number of CD11c+, OX-62+, CD4+, and CD8 + cells significantly increased in the RFA-OK-432 group compared to the RFA-only group. RFA with intratumoural OK-432 injection resulted in distant tumour suppression, prolonged survival, and increased dendritic cells, cytotoxic T cells, IFN-γ, and TNF-α, whereas RFA or OK-432 alone did not produce this effect. This combination may induce an abscopal effect in human osteosarcoma. |
| doi_str_mv | 10.1038/s41598-020-66934-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7296025</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2414006907</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-c4e249aae39f5a5306d627ded557d481e84930173d9a4a534226584ff7a2c6fc3</originalsourceid><addsrcrecordid>eNp9kctu1DAUhi1ERavSF2CBLLFhE_A98QYJVdAiKrWLsrY8jj3jURIPtgPqS_DMnJm0pbDAC9_Od36f4x-hV5S8o4R374ugUncNYaRRSnPRqGfohBEhG8YZe_5kf4zOStkSGJJpQfULdMyZhI2gJ-jXTU5jLHFaY7sqLu3sgH0I3lWcAnZpXMXJ1pgmXDc-290d_hnr5nAYAa3zmOYMqcMC2anHcarZLgEgrr82gjO43ILmHokHKQwITqX6VGyGZyweU--Hl-go2KH4s_v1FH37_On2_LK5ur74cv7xqnGiFRVmz4S21nMdpJWcqF6xtve9lG0vOuo7oTmhLe-1FRAXjCnZiRBay5wKjp-iD4vubl6Nvnd-X_NgdjmONt-ZZKP5OzLFjVmnH6ZlWhEmQeDtvUBO32dfqoFfdH4Y7OTTXAwTVBCiNGkBffMPuoWvmaC9PcUZp2ANUGyhXE6lZB8ei6HE7B03i-MGHDcHx42CpNdP23hMefAXAL4ABULT2uc_b_9H9jeh2rkm</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2413231000</pqid></control><display><type>article</type><title>Promising abscopal effect of combination therapy with thermal tumour ablation and intratumoural OK-432 injection in the rat osteosarcoma model</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Iwai, Tadashi ; Oebisu, Naoto ; Hoshi, Manabu ; Orita, Kumi ; Yamamoto, Akira ; Hamamoto, Shinichi ; Kageyama, Ken ; Nakamura, Hiroaki</creator><creatorcontrib>Iwai, Tadashi ; Oebisu, Naoto ; Hoshi, Manabu ; Orita, Kumi ; Yamamoto, Akira ; Hamamoto, Shinichi ; Kageyama, Ken ; Nakamura, Hiroaki</creatorcontrib><description>Treatment options for metastatic osteosarcoma are limited. The present study aimed to evaluate whether radiofrequency ablation (RFA) combined with intratumoural OK-432 injection induces systemic anti-tumour immunity in rat osteosarcoma model. Eighty of 145 rats were assigned to four groups to evaluate overall survival and tumour size: control (no treatment), RFA-only, OK-432, and RFA-OK-432. The remaining 65 were assigned for histological examination. Maximum diameters of tibial and lung tumours were determined. Tumour samples were histologically examined using haematoxylin-eosin and immunohistochemical staining. Overall survival was significantly prolonged in the RFA-OK-432 group compared to the RFA-only and OK-432 groups. Only rats in the RFA-OK-432 group exhibited significant decreases in maximum tumour diameter after treatment. Ki-67-positive tumour cells in the RFA-OK-432 group were significantly stained negative on immunohistochemical analysis as opposed to those in the RFA-only and OK-432 groups. The number of CD11c+, OX-62+, CD4+, and CD8 + cells significantly increased in the RFA-OK-432 group compared to the RFA-only group. RFA with intratumoural OK-432 injection resulted in distant tumour suppression, prolonged survival, and increased dendritic cells, cytotoxic T cells, IFN-γ, and TNF-α, whereas RFA or OK-432 alone did not produce this effect. This combination may induce an abscopal effect in human osteosarcoma.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-66934-6</identifier><identifier>PMID: 32541941</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/67 ; 692/4028 ; Ablation ; Animals ; Antineoplastic Agents - administration & dosage ; Antineoplastic Agents - pharmacology ; Bone cancer ; Bone Neoplasms - pathology ; Bone Neoplasms - therapy ; CD11c antigen ; CD4 antigen ; CD8 antigen ; Cell Line, Tumor ; Combination therapy ; Combined Modality Therapy ; Cytotoxicity ; Dendritic cells ; Humanities and Social Sciences ; Injection ; Lymphocytes T ; Metastases ; multidisciplinary ; Osteosarcoma ; Osteosarcoma - pathology ; Osteosarcoma - therapy ; Picibanil - administration & dosage ; Picibanil - pharmacology ; Radiofrequency Ablation - methods ; Rats ; Sarcoma ; Science ; Science (multidisciplinary) ; Treatment Outcome ; Tumor Burden ; Tumor necrosis factor-α ; Tumors ; Xenograft Model Antitumor Assays ; γ-Interferon</subject><ispartof>Scientific reports, 2020-06, Vol.10 (1), p.9679-9679, Article 9679</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-c4e249aae39f5a5306d627ded557d481e84930173d9a4a534226584ff7a2c6fc3</citedby><cites>FETCH-LOGICAL-c474t-c4e249aae39f5a5306d627ded557d481e84930173d9a4a534226584ff7a2c6fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296025/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296025/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32541941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Iwai, Tadashi</creatorcontrib><creatorcontrib>Oebisu, Naoto</creatorcontrib><creatorcontrib>Hoshi, Manabu</creatorcontrib><creatorcontrib>Orita, Kumi</creatorcontrib><creatorcontrib>Yamamoto, Akira</creatorcontrib><creatorcontrib>Hamamoto, Shinichi</creatorcontrib><creatorcontrib>Kageyama, Ken</creatorcontrib><creatorcontrib>Nakamura, Hiroaki</creatorcontrib><title>Promising abscopal effect of combination therapy with thermal tumour ablation and intratumoural OK-432 injection in the rat osteosarcoma model</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Treatment options for metastatic osteosarcoma are limited. The present study aimed to evaluate whether radiofrequency ablation (RFA) combined with intratumoural OK-432 injection induces systemic anti-tumour immunity in rat osteosarcoma model. Eighty of 145 rats were assigned to four groups to evaluate overall survival and tumour size: control (no treatment), RFA-only, OK-432, and RFA-OK-432. The remaining 65 were assigned for histological examination. Maximum diameters of tibial and lung tumours were determined. Tumour samples were histologically examined using haematoxylin-eosin and immunohistochemical staining. Overall survival was significantly prolonged in the RFA-OK-432 group compared to the RFA-only and OK-432 groups. Only rats in the RFA-OK-432 group exhibited significant decreases in maximum tumour diameter after treatment. Ki-67-positive tumour cells in the RFA-OK-432 group were significantly stained negative on immunohistochemical analysis as opposed to those in the RFA-only and OK-432 groups. The number of CD11c+, OX-62+, CD4+, and CD8 + cells significantly increased in the RFA-OK-432 group compared to the RFA-only group. RFA with intratumoural OK-432 injection resulted in distant tumour suppression, prolonged survival, and increased dendritic cells, cytotoxic T cells, IFN-γ, and TNF-α, whereas RFA or OK-432 alone did not produce this effect. This combination may induce an abscopal effect in human osteosarcoma.</description><subject>631/67</subject><subject>692/4028</subject><subject>Ablation</subject><subject>Animals</subject><subject>Antineoplastic Agents - administration & dosage</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Bone cancer</subject><subject>Bone Neoplasms - pathology</subject><subject>Bone Neoplasms - therapy</subject><subject>CD11c antigen</subject><subject>CD4 antigen</subject><subject>CD8 antigen</subject><subject>Cell Line, Tumor</subject><subject>Combination therapy</subject><subject>Combined Modality Therapy</subject><subject>Cytotoxicity</subject><subject>Dendritic cells</subject><subject>Humanities and Social Sciences</subject><subject>Injection</subject><subject>Lymphocytes T</subject><subject>Metastases</subject><subject>multidisciplinary</subject><subject>Osteosarcoma</subject><subject>Osteosarcoma - pathology</subject><subject>Osteosarcoma - therapy</subject><subject>Picibanil - administration & dosage</subject><subject>Picibanil - pharmacology</subject><subject>Radiofrequency Ablation - methods</subject><subject>Rats</subject><subject>Sarcoma</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Treatment Outcome</subject><subject>Tumor Burden</subject><subject>Tumor necrosis factor-α</subject><subject>Tumors</subject><subject>Xenograft Model Antitumor Assays</subject><subject>γ-Interferon</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kctu1DAUhi1ERavSF2CBLLFhE_A98QYJVdAiKrWLsrY8jj3jURIPtgPqS_DMnJm0pbDAC9_Od36f4x-hV5S8o4R374ugUncNYaRRSnPRqGfohBEhG8YZe_5kf4zOStkSGJJpQfULdMyZhI2gJ-jXTU5jLHFaY7sqLu3sgH0I3lWcAnZpXMXJ1pgmXDc-290d_hnr5nAYAa3zmOYMqcMC2anHcarZLgEgrr82gjO43ILmHokHKQwITqX6VGyGZyweU--Hl-go2KH4s_v1FH37_On2_LK5ur74cv7xqnGiFRVmz4S21nMdpJWcqF6xtve9lG0vOuo7oTmhLe-1FRAXjCnZiRBay5wKjp-iD4vubl6Nvnd-X_NgdjmONt-ZZKP5OzLFjVmnH6ZlWhEmQeDtvUBO32dfqoFfdH4Y7OTTXAwTVBCiNGkBffMPuoWvmaC9PcUZp2ANUGyhXE6lZB8ei6HE7B03i-MGHDcHx42CpNdP23hMefAXAL4ABULT2uc_b_9H9jeh2rkm</recordid><startdate>20200615</startdate><enddate>20200615</enddate><creator>Iwai, Tadashi</creator><creator>Oebisu, Naoto</creator><creator>Hoshi, Manabu</creator><creator>Orita, Kumi</creator><creator>Yamamoto, Akira</creator><creator>Hamamoto, Shinichi</creator><creator>Kageyama, Ken</creator><creator>Nakamura, Hiroaki</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200615</creationdate><title>Promising abscopal effect of combination therapy with thermal tumour ablation and intratumoural OK-432 injection in the rat osteosarcoma model</title><author>Iwai, Tadashi ; Oebisu, Naoto ; Hoshi, Manabu ; Orita, Kumi ; Yamamoto, Akira ; Hamamoto, Shinichi ; Kageyama, Ken ; Nakamura, Hiroaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-c4e249aae39f5a5306d627ded557d481e84930173d9a4a534226584ff7a2c6fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>631/67</topic><topic>692/4028</topic><topic>Ablation</topic><topic>Animals</topic><topic>Antineoplastic Agents - administration & dosage</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Bone cancer</topic><topic>Bone Neoplasms - pathology</topic><topic>Bone Neoplasms - therapy</topic><topic>CD11c antigen</topic><topic>CD4 antigen</topic><topic>CD8 antigen</topic><topic>Cell Line, Tumor</topic><topic>Combination therapy</topic><topic>Combined Modality Therapy</topic><topic>Cytotoxicity</topic><topic>Dendritic cells</topic><topic>Humanities and Social Sciences</topic><topic>Injection</topic><topic>Lymphocytes T</topic><topic>Metastases</topic><topic>multidisciplinary</topic><topic>Osteosarcoma</topic><topic>Osteosarcoma - pathology</topic><topic>Osteosarcoma - therapy</topic><topic>Picibanil - administration & dosage</topic><topic>Picibanil - pharmacology</topic><topic>Radiofrequency Ablation - methods</topic><topic>Rats</topic><topic>Sarcoma</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Treatment Outcome</topic><topic>Tumor Burden</topic><topic>Tumor necrosis factor-α</topic><topic>Tumors</topic><topic>Xenograft Model Antitumor Assays</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iwai, Tadashi</creatorcontrib><creatorcontrib>Oebisu, Naoto</creatorcontrib><creatorcontrib>Hoshi, Manabu</creatorcontrib><creatorcontrib>Orita, Kumi</creatorcontrib><creatorcontrib>Yamamoto, Akira</creatorcontrib><creatorcontrib>Hamamoto, Shinichi</creatorcontrib><creatorcontrib>Kageyama, Ken</creatorcontrib><creatorcontrib>Nakamura, Hiroaki</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iwai, Tadashi</au><au>Oebisu, Naoto</au><au>Hoshi, Manabu</au><au>Orita, Kumi</au><au>Yamamoto, Akira</au><au>Hamamoto, Shinichi</au><au>Kageyama, Ken</au><au>Nakamura, Hiroaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Promising abscopal effect of combination therapy with thermal tumour ablation and intratumoural OK-432 injection in the rat osteosarcoma model</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-06-15</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>9679</spage><epage>9679</epage><pages>9679-9679</pages><artnum>9679</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Treatment options for metastatic osteosarcoma are limited. The present study aimed to evaluate whether radiofrequency ablation (RFA) combined with intratumoural OK-432 injection induces systemic anti-tumour immunity in rat osteosarcoma model. Eighty of 145 rats were assigned to four groups to evaluate overall survival and tumour size: control (no treatment), RFA-only, OK-432, and RFA-OK-432. The remaining 65 were assigned for histological examination. Maximum diameters of tibial and lung tumours were determined. Tumour samples were histologically examined using haematoxylin-eosin and immunohistochemical staining. Overall survival was significantly prolonged in the RFA-OK-432 group compared to the RFA-only and OK-432 groups. Only rats in the RFA-OK-432 group exhibited significant decreases in maximum tumour diameter after treatment. Ki-67-positive tumour cells in the RFA-OK-432 group were significantly stained negative on immunohistochemical analysis as opposed to those in the RFA-only and OK-432 groups. The number of CD11c+, OX-62+, CD4+, and CD8 + cells significantly increased in the RFA-OK-432 group compared to the RFA-only group. RFA with intratumoural OK-432 injection resulted in distant tumour suppression, prolonged survival, and increased dendritic cells, cytotoxic T cells, IFN-γ, and TNF-α, whereas RFA or OK-432 alone did not produce this effect. This combination may induce an abscopal effect in human osteosarcoma.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32541941</pmid><doi>10.1038/s41598-020-66934-6</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2020-06, Vol.10 (1), p.9679-9679, Article 9679 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7296025 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature OA Free Journals; Nature Free; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 631/67 692/4028 Ablation Animals Antineoplastic Agents - administration & dosage Antineoplastic Agents - pharmacology Bone cancer Bone Neoplasms - pathology Bone Neoplasms - therapy CD11c antigen CD4 antigen CD8 antigen Cell Line, Tumor Combination therapy Combined Modality Therapy Cytotoxicity Dendritic cells Humanities and Social Sciences Injection Lymphocytes T Metastases multidisciplinary Osteosarcoma Osteosarcoma - pathology Osteosarcoma - therapy Picibanil - administration & dosage Picibanil - pharmacology Radiofrequency Ablation - methods Rats Sarcoma Science Science (multidisciplinary) Treatment Outcome Tumor Burden Tumor necrosis factor-α Tumors Xenograft Model Antitumor Assays γ-Interferon |
| title | Promising abscopal effect of combination therapy with thermal tumour ablation and intratumoural OK-432 injection in the rat osteosarcoma model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T04%3A26%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Promising%20abscopal%20effect%20of%20combination%20therapy%20with%20thermal%20tumour%20ablation%20and%20intratumoural%20OK-432%20injection%20in%20the%20rat%20osteosarcoma%20model&rft.jtitle=Scientific%20reports&rft.au=Iwai,%20Tadashi&rft.date=2020-06-15&rft.volume=10&rft.issue=1&rft.spage=9679&rft.epage=9679&rft.pages=9679-9679&rft.artnum=9679&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-020-66934-6&rft_dat=%3Cproquest_pubme%3E2414006907%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2413231000&rft_id=info:pmid/32541941&rfr_iscdi=true |