Gastroprotective effect of phytoncide extract from Pinus koraiensis pinecone in Helicobacter pylori infection
For centuries, herbs have been used by traditional therapists around the world to treat gastrointestinal tract disorders, such as gastritis. We hypothesized that the anti- Helicobacter pylori properties of phytoncide, which is extracted from pinecone waste, would facilitate use as a natural gastropr...
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| description | For centuries, herbs have been used by traditional therapists around the world to treat gastrointestinal tract disorders, such as gastritis. We hypothesized that the anti-
Helicobacter pylori
properties of phytoncide, which is extracted from pinecone waste, would facilitate use as a natural gastroprotective product to treat gastrointestinal tract disorders. Thus, we investigated
in vitro
antibacterial efficacy against
H. pylori
by agar diffusion assay. To determine the gastroprotective properties of phytoncide, we conducted hematoxylin and eosin staining, performed assays for the detection of the cytotoxin gene, and evaluated pro-inflammatory cytokine expression in
H. pylori
-infected C57BL/6 mice. Phytoncide significantly inhibited the survival of
H. pylor
i in the gastrointestinal system of C57BL/6 mice. Reduction of gastric severity in
H. pylori
-infected mice was associated with reductions in the expression levels of pro-inflammatory cytokines in the gastric mucosa, and of the cytotoxin CagA gene in phytoncide treated groups (
P
|
| doi_str_mv | 10.1038/s41598-020-66603-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7293342</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2412985070</sourcerecordid><originalsourceid>FETCH-LOGICAL-c577t-ec6c48de75881e44dd368a82b7c93e039ea95ff106a028b457904c8f75079add3</originalsourceid><addsrcrecordid>eNp9kc1u1TAQhS0EolXpC7BAltiwCTj-SewNEqqgrVQJFrC2fJ1x65LYwU6q3rdnbm9pCwu88Wjm85kZH0Jet-x9y4T-UGWrjG4YZ03XdUw0-hk55EyqhgvOnz-JD8hxrdcMj-JGtuYlORBcCcEEPyTTqatLyXPJC_gl3gCFEDCiOdD5arvk5OOAydulOMyGkif6Laa10p-5uAipxkrnmMDnBDQmegZj9HmDMBQ6b8dcIqZ3kjGnV-RFcGOF4_v7iPz48vn7yVlz8fX0_OTTReNV3y8N-M5LPUCvtG5BymEQnXaab3pvBDBhwBkVQss6x7jeSNUbJr0OvWK9cUgfkY973XndTDB4SDj-aOcSJ1e2Nrto_66keGUv843tuRFCchR4dy9Q8q8V6mKnWD2Mo0uQ12q5bLnR2I4h-vYf9DqvJeF6dxR-uOo0UnxP-ZJrLRAehmmZ3Rlq94ZaNNTeGWp3j948XePhyR_7EBB7oGIpXUJ57P0f2d_XW64c</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2412419568</pqid></control><display><type>article</type><title>Gastroprotective effect of phytoncide extract from Pinus koraiensis pinecone in Helicobacter pylori infection</title><source>MEDLINE</source><source>Nature Free</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature OA Free Journals</source><creator>Kim, Se-eun ; Memon, Azra ; Kim, Bae Yong ; Jeon, Hyelin ; Lee, Woon Kyu ; Kang, Se Chan</creator><creatorcontrib>Kim, Se-eun ; Memon, Azra ; Kim, Bae Yong ; Jeon, Hyelin ; Lee, Woon Kyu ; Kang, Se Chan</creatorcontrib><description>For centuries, herbs have been used by traditional therapists around the world to treat gastrointestinal tract disorders, such as gastritis. We hypothesized that the anti-
Helicobacter pylori
properties of phytoncide, which is extracted from pinecone waste, would facilitate use as a natural gastroprotective product to treat gastrointestinal tract disorders. Thus, we investigated
in vitro
antibacterial efficacy against
H. pylori
by agar diffusion assay. To determine the gastroprotective properties of phytoncide, we conducted hematoxylin and eosin staining, performed assays for the detection of the cytotoxin gene, and evaluated pro-inflammatory cytokine expression in
H. pylori
-infected C57BL/6 mice. Phytoncide significantly inhibited the survival of
H. pylor
i in the gastrointestinal system of C57BL/6 mice. Reduction of gastric severity in
H. pylori
-infected mice was associated with reductions in the expression levels of pro-inflammatory cytokines in the gastric mucosa, and of the cytotoxin CagA gene in phytoncide treated groups (
P
< 0.05 and
P
< 0.01). In conclusion, phytoncide significantly inhibited the growth of
H. pylori
in gastro tissue, possibly due to the abundant α-pinene present in the phytoncide as detected by HPLC analysis. Further studies are needed to validate our findings, but we suggest that phytoncide has the potential to be used as a natural ingredient in anti-
H. pylori
products.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-66603-8</identifier><identifier>PMID: 32533032</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 13/1 ; 59 ; 631/1647/767/2199 ; 64 ; 64/60 ; 692/699/1503/1501 ; 82 ; 82/1 ; Amoxicillin - pharmacology ; Amoxicillin - therapeutic use ; Animals ; Anti-Bacterial Agents - isolation & purification ; Anti-Bacterial Agents - pharmacology ; Anti-Bacterial Agents - therapeutic use ; Antibodies ; Antibodies, Bacterial - blood ; Antigens, Bacterial - biosynthesis ; Antigens, Bacterial - genetics ; Antimicrobial agents ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; CagA protein ; Chromatography, High Pressure Liquid ; Clarithromycin - pharmacology ; Clarithromycin - therapeutic use ; Cytokines ; Cytokines - biosynthesis ; Cytokines - genetics ; DNA, Bacterial - genetics ; Drug Evaluation, Preclinical ; Experiments ; Flowers & plants ; Flowers - chemistry ; Gastric mucosa ; Gastric Mucosa - drug effects ; Gastric Mucosa - metabolism ; Gastritis ; Gastritis - microbiology ; Gastritis - prevention & control ; Gastrointestinal diseases ; Gastrointestinal tract ; Gene expression ; Glycyrrhiza ; Helicobacter Infections - drug therapy ; Helicobacter Infections - prevention & control ; Helicobacter pylori ; Helicobacter pylori - drug effects ; Helicobacter pylori - genetics ; Helicobacter pylori - immunology ; High-performance liquid chromatography ; Humanities and Social Sciences ; Immunoglobulin G - blood ; Infections ; Inflammation ; Liquid chromatography ; Male ; Medical research ; Mice ; Mice, Inbred C57BL ; Monoterpenes - isolation & purification ; Monoterpenes - pharmacology ; Monoterpenes - therapeutic use ; multidisciplinary ; Natural gas ; Omeprazole - pharmacology ; Omeprazole - therapeutic use ; Pinus - chemistry ; Plant Extracts - therapeutic use ; Science ; Science (multidisciplinary) ; Specific Pathogen-Free Organisms ; α-Pinene</subject><ispartof>Scientific reports, 2020-06, Vol.10 (1), p.9547-9547, Article 9547</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c577t-ec6c48de75881e44dd368a82b7c93e039ea95ff106a028b457904c8f75079add3</citedby><cites>FETCH-LOGICAL-c577t-ec6c48de75881e44dd368a82b7c93e039ea95ff106a028b457904c8f75079add3</cites><orcidid>0000-0001-9469-3677</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293342/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293342/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32533032$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Se-eun</creatorcontrib><creatorcontrib>Memon, Azra</creatorcontrib><creatorcontrib>Kim, Bae Yong</creatorcontrib><creatorcontrib>Jeon, Hyelin</creatorcontrib><creatorcontrib>Lee, Woon Kyu</creatorcontrib><creatorcontrib>Kang, Se Chan</creatorcontrib><title>Gastroprotective effect of phytoncide extract from Pinus koraiensis pinecone in Helicobacter pylori infection</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>For centuries, herbs have been used by traditional therapists around the world to treat gastrointestinal tract disorders, such as gastritis. We hypothesized that the anti-
Helicobacter pylori
properties of phytoncide, which is extracted from pinecone waste, would facilitate use as a natural gastroprotective product to treat gastrointestinal tract disorders. Thus, we investigated
in vitro
antibacterial efficacy against
H. pylori
by agar diffusion assay. To determine the gastroprotective properties of phytoncide, we conducted hematoxylin and eosin staining, performed assays for the detection of the cytotoxin gene, and evaluated pro-inflammatory cytokine expression in
H. pylori
-infected C57BL/6 mice. Phytoncide significantly inhibited the survival of
H. pylor
i in the gastrointestinal system of C57BL/6 mice. Reduction of gastric severity in
H. pylori
-infected mice was associated with reductions in the expression levels of pro-inflammatory cytokines in the gastric mucosa, and of the cytotoxin CagA gene in phytoncide treated groups (
P
< 0.05 and
P
< 0.01). In conclusion, phytoncide significantly inhibited the growth of
H. pylori
in gastro tissue, possibly due to the abundant α-pinene present in the phytoncide as detected by HPLC analysis. Further studies are needed to validate our findings, but we suggest that phytoncide has the potential to be used as a natural ingredient in anti-
H. pylori
products.</description><subject>13</subject><subject>13/1</subject><subject>59</subject><subject>631/1647/767/2199</subject><subject>64</subject><subject>64/60</subject><subject>692/699/1503/1501</subject><subject>82</subject><subject>82/1</subject><subject>Amoxicillin - pharmacology</subject><subject>Amoxicillin - therapeutic use</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - isolation & purification</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Antibodies</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antigens, Bacterial - biosynthesis</subject><subject>Antigens, Bacterial - genetics</subject><subject>Antimicrobial agents</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>CagA protein</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Clarithromycin - pharmacology</subject><subject>Clarithromycin - therapeutic use</subject><subject>Cytokines</subject><subject>Cytokines - biosynthesis</subject><subject>Cytokines - genetics</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug Evaluation, Preclinical</subject><subject>Experiments</subject><subject>Flowers & plants</subject><subject>Flowers - chemistry</subject><subject>Gastric mucosa</subject><subject>Gastric Mucosa - drug effects</subject><subject>Gastric Mucosa - metabolism</subject><subject>Gastritis</subject><subject>Gastritis - microbiology</subject><subject>Gastritis - prevention & control</subject><subject>Gastrointestinal diseases</subject><subject>Gastrointestinal tract</subject><subject>Gene expression</subject><subject>Glycyrrhiza</subject><subject>Helicobacter Infections - drug therapy</subject><subject>Helicobacter Infections - prevention & control</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - drug effects</subject><subject>Helicobacter pylori - genetics</subject><subject>Helicobacter pylori - immunology</subject><subject>High-performance liquid chromatography</subject><subject>Humanities and Social Sciences</subject><subject>Immunoglobulin G - blood</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Liquid chromatography</subject><subject>Male</subject><subject>Medical research</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Monoterpenes - isolation & purification</subject><subject>Monoterpenes - pharmacology</subject><subject>Monoterpenes - therapeutic use</subject><subject>multidisciplinary</subject><subject>Natural gas</subject><subject>Omeprazole - pharmacology</subject><subject>Omeprazole - therapeutic use</subject><subject>Pinus - chemistry</subject><subject>Plant Extracts - therapeutic use</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Specific Pathogen-Free Organisms</subject><subject>α-Pinene</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1u1TAQhS0EolXpC7BAltiwCTj-SewNEqqgrVQJFrC2fJ1x65LYwU6q3rdnbm9pCwu88Wjm85kZH0Jet-x9y4T-UGWrjG4YZ03XdUw0-hk55EyqhgvOnz-JD8hxrdcMj-JGtuYlORBcCcEEPyTTqatLyXPJC_gl3gCFEDCiOdD5arvk5OOAydulOMyGkif6Laa10p-5uAipxkrnmMDnBDQmegZj9HmDMBQ6b8dcIqZ3kjGnV-RFcGOF4_v7iPz48vn7yVlz8fX0_OTTReNV3y8N-M5LPUCvtG5BymEQnXaab3pvBDBhwBkVQss6x7jeSNUbJr0OvWK9cUgfkY973XndTDB4SDj-aOcSJ1e2Nrto_66keGUv843tuRFCchR4dy9Q8q8V6mKnWD2Mo0uQ12q5bLnR2I4h-vYf9DqvJeF6dxR-uOo0UnxP-ZJrLRAehmmZ3Rlq94ZaNNTeGWp3j948XePhyR_7EBB7oGIpXUJ57P0f2d_XW64c</recordid><startdate>20200612</startdate><enddate>20200612</enddate><creator>Kim, Se-eun</creator><creator>Memon, Azra</creator><creator>Kim, Bae Yong</creator><creator>Jeon, Hyelin</creator><creator>Lee, Woon Kyu</creator><creator>Kang, Se Chan</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9469-3677</orcidid></search><sort><creationdate>20200612</creationdate><title>Gastroprotective effect of phytoncide extract from Pinus koraiensis pinecone in Helicobacter pylori infection</title><author>Kim, Se-eun ; Memon, Azra ; Kim, Bae Yong ; Jeon, Hyelin ; Lee, Woon Kyu ; Kang, Se Chan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c577t-ec6c48de75881e44dd368a82b7c93e039ea95ff106a028b457904c8f75079add3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>13</topic><topic>13/1</topic><topic>59</topic><topic>631/1647/767/2199</topic><topic>64</topic><topic>64/60</topic><topic>692/699/1503/1501</topic><topic>82</topic><topic>82/1</topic><topic>Amoxicillin - pharmacology</topic><topic>Amoxicillin - therapeutic use</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - isolation & purification</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Anti-Bacterial Agents - therapeutic use</topic><topic>Antibodies</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antigens, Bacterial - biosynthesis</topic><topic>Antigens, Bacterial - genetics</topic><topic>Antimicrobial agents</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>CagA protein</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Clarithromycin - pharmacology</topic><topic>Clarithromycin - therapeutic use</topic><topic>Cytokines</topic><topic>Cytokines - biosynthesis</topic><topic>Cytokines - genetics</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug Evaluation, Preclinical</topic><topic>Experiments</topic><topic>Flowers & plants</topic><topic>Flowers - chemistry</topic><topic>Gastric mucosa</topic><topic>Gastric Mucosa - drug effects</topic><topic>Gastric Mucosa - metabolism</topic><topic>Gastritis</topic><topic>Gastritis - microbiology</topic><topic>Gastritis - prevention & control</topic><topic>Gastrointestinal diseases</topic><topic>Gastrointestinal tract</topic><topic>Gene expression</topic><topic>Glycyrrhiza</topic><topic>Helicobacter Infections - drug therapy</topic><topic>Helicobacter Infections - prevention & control</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - drug effects</topic><topic>Helicobacter pylori - genetics</topic><topic>Helicobacter pylori - immunology</topic><topic>High-performance liquid chromatography</topic><topic>Humanities and Social Sciences</topic><topic>Immunoglobulin G - blood</topic><topic>Infections</topic><topic>Inflammation</topic><topic>Liquid chromatography</topic><topic>Male</topic><topic>Medical research</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Monoterpenes - isolation & purification</topic><topic>Monoterpenes - pharmacology</topic><topic>Monoterpenes - therapeutic use</topic><topic>multidisciplinary</topic><topic>Natural gas</topic><topic>Omeprazole - pharmacology</topic><topic>Omeprazole - therapeutic use</topic><topic>Pinus - chemistry</topic><topic>Plant Extracts - therapeutic use</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Specific Pathogen-Free Organisms</topic><topic>α-Pinene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Se-eun</creatorcontrib><creatorcontrib>Memon, Azra</creatorcontrib><creatorcontrib>Kim, Bae Yong</creatorcontrib><creatorcontrib>Jeon, Hyelin</creatorcontrib><creatorcontrib>Lee, Woon Kyu</creatorcontrib><creatorcontrib>Kang, Se Chan</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Se-eun</au><au>Memon, Azra</au><au>Kim, Bae Yong</au><au>Jeon, Hyelin</au><au>Lee, Woon Kyu</au><au>Kang, Se Chan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gastroprotective effect of phytoncide extract from Pinus koraiensis pinecone in Helicobacter pylori infection</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-06-12</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>9547</spage><epage>9547</epage><pages>9547-9547</pages><artnum>9547</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>For centuries, herbs have been used by traditional therapists around the world to treat gastrointestinal tract disorders, such as gastritis. We hypothesized that the anti-
Helicobacter pylori
properties of phytoncide, which is extracted from pinecone waste, would facilitate use as a natural gastroprotective product to treat gastrointestinal tract disorders. Thus, we investigated
in vitro
antibacterial efficacy against
H. pylori
by agar diffusion assay. To determine the gastroprotective properties of phytoncide, we conducted hematoxylin and eosin staining, performed assays for the detection of the cytotoxin gene, and evaluated pro-inflammatory cytokine expression in
H. pylori
-infected C57BL/6 mice. Phytoncide significantly inhibited the survival of
H. pylor
i in the gastrointestinal system of C57BL/6 mice. Reduction of gastric severity in
H. pylori
-infected mice was associated with reductions in the expression levels of pro-inflammatory cytokines in the gastric mucosa, and of the cytotoxin CagA gene in phytoncide treated groups (
P
< 0.05 and
P
< 0.01). In conclusion, phytoncide significantly inhibited the growth of
H. pylori
in gastro tissue, possibly due to the abundant α-pinene present in the phytoncide as detected by HPLC analysis. Further studies are needed to validate our findings, but we suggest that phytoncide has the potential to be used as a natural ingredient in anti-
H. pylori
products.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32533032</pmid><doi>10.1038/s41598-020-66603-8</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-9469-3677</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Nature Free; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 13 13/1 59 631/1647/767/2199 64 64/60 692/699/1503/1501 82 82/1 Amoxicillin - pharmacology Amoxicillin - therapeutic use Animals Anti-Bacterial Agents - isolation & purification Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - therapeutic use Antibodies Antibodies, Bacterial - blood Antigens, Bacterial - biosynthesis Antigens, Bacterial - genetics Antimicrobial agents Bacterial Proteins - biosynthesis Bacterial Proteins - genetics CagA protein Chromatography, High Pressure Liquid Clarithromycin - pharmacology Clarithromycin - therapeutic use Cytokines Cytokines - biosynthesis Cytokines - genetics DNA, Bacterial - genetics Drug Evaluation, Preclinical Experiments Flowers & plants Flowers - chemistry Gastric mucosa Gastric Mucosa - drug effects Gastric Mucosa - metabolism Gastritis Gastritis - microbiology Gastritis - prevention & control Gastrointestinal diseases Gastrointestinal tract Gene expression Glycyrrhiza Helicobacter Infections - drug therapy Helicobacter Infections - prevention & control Helicobacter pylori Helicobacter pylori - drug effects Helicobacter pylori - genetics Helicobacter pylori - immunology High-performance liquid chromatography Humanities and Social Sciences Immunoglobulin G - blood Infections Inflammation Liquid chromatography Male Medical research Mice Mice, Inbred C57BL Monoterpenes - isolation & purification Monoterpenes - pharmacology Monoterpenes - therapeutic use multidisciplinary Natural gas Omeprazole - pharmacology Omeprazole - therapeutic use Pinus - chemistry Plant Extracts - therapeutic use Science Science (multidisciplinary) Specific Pathogen-Free Organisms α-Pinene |
| title | Gastroprotective effect of phytoncide extract from Pinus koraiensis pinecone in Helicobacter pylori infection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T04%3A04%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Gastroprotective%20effect%20of%20phytoncide%20extract%20from%20Pinus%20koraiensis%20pinecone%20in%20Helicobacter%20pylori%20infection&rft.jtitle=Scientific%20reports&rft.au=Kim,%20Se-eun&rft.date=2020-06-12&rft.volume=10&rft.issue=1&rft.spage=9547&rft.epage=9547&rft.pages=9547-9547&rft.artnum=9547&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-020-66603-8&rft_dat=%3Cproquest_pubme%3E2412985070%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2412419568&rft_id=info:pmid/32533032&rfr_iscdi=true |