Loss of hypoxia-inducible factor 1α affects hypoxia tolerance in larval and adult zebrafish ( Danio rerio )
The coordination of the hypoxic response is attributed, in part, to hypoxia-inducible factor 1α (Hif-1α), a regulator of hypoxia-induced transcription. After the teleost-specific genome duplication, most teleost fishes lost the duplicate copy of Hif-1α, except species in the cyprinid lineage that re...
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creator | Mandic, Milica Best, Carol Perry, Steve F |
description | The coordination of the hypoxic response is attributed, in part, to hypoxia-inducible factor 1α (Hif-1α), a regulator of hypoxia-induced transcription. After the teleost-specific genome duplication, most teleost fishes lost the duplicate copy of Hif-1α, except species in the cyprinid lineage that retained both paralogues of Hif-1α (Hif1aa and Hif1ab). Little is known about the contribution of Hif-1α, and specifically of each paralogue, to hypoxia tolerance. Here, we examined hypoxia tolerance in wild-type (Hif1aa
ab
) and Hif-1α knockout lines (Hif1aa
; Hif1ab
; Hif1aa
ab
) of zebrafish (
). Critical O
tension (
; the partial pressure of oxygen (PO
) at which O
consumption can no longer be maintained) and time to loss of equilibrium (LOE), two indices of hypoxia tolerance, were assessed in larvae and adults. Knockout of both paralogues significantly increased
(decreased hypoxia tolerance) in larval fish. Prior exposure of larvae to hypoxia decreased
in wild-type fish, an effect mediated by the Hif1aa paralogue. In adults, individuals with a knockout of either paralogue exhibited significantly decreased time to LOE but no difference in
. Together, these results demonstrate that in zebrafish, tolerance to hypoxia and improved hypoxia tolerance after pre-exposure to hypoxia (pre-conditioning) are mediated, at least in part, by Hif-1α. |
doi_str_mv | 10.1098/rspb.2020.0798 |
format | Article |
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ab
) and Hif-1α knockout lines (Hif1aa
; Hif1ab
; Hif1aa
ab
) of zebrafish (
). Critical O
tension (
; the partial pressure of oxygen (PO
) at which O
consumption can no longer be maintained) and time to loss of equilibrium (LOE), two indices of hypoxia tolerance, were assessed in larvae and adults. Knockout of both paralogues significantly increased
(decreased hypoxia tolerance) in larval fish. Prior exposure of larvae to hypoxia decreased
in wild-type fish, an effect mediated by the Hif1aa paralogue. In adults, individuals with a knockout of either paralogue exhibited significantly decreased time to LOE but no difference in
. Together, these results demonstrate that in zebrafish, tolerance to hypoxia and improved hypoxia tolerance after pre-exposure to hypoxia (pre-conditioning) are mediated, at least in part, by Hif-1α.</description><identifier>ISSN: 0962-8452</identifier><identifier>EISSN: 1471-2954</identifier><identifier>DOI: 10.1098/rspb.2020.0798</identifier><identifier>PMID: 32453991</identifier><language>eng</language><publisher>England: The Royal Society</publisher><subject>Development and Physiology</subject><ispartof>Proceedings of the Royal Society. B, Biological sciences, 2020-05, Vol.287 (1927), p.20200798-20200798</ispartof><rights>2020 The Author(s) 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-7f5a8ad39534f73ffee2030a1949370e368858572f51f5545d48797e0aa7ae7d3</citedby><cites>FETCH-LOGICAL-c434t-7f5a8ad39534f73ffee2030a1949370e368858572f51f5545d48797e0aa7ae7d3</cites><orcidid>0000-0002-9377-4173</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287360/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287360/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32453991$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mandic, Milica</creatorcontrib><creatorcontrib>Best, Carol</creatorcontrib><creatorcontrib>Perry, Steve F</creatorcontrib><title>Loss of hypoxia-inducible factor 1α affects hypoxia tolerance in larval and adult zebrafish ( Danio rerio )</title><title>Proceedings of the Royal Society. B, Biological sciences</title><addtitle>Proc Biol Sci</addtitle><description>The coordination of the hypoxic response is attributed, in part, to hypoxia-inducible factor 1α (Hif-1α), a regulator of hypoxia-induced transcription. After the teleost-specific genome duplication, most teleost fishes lost the duplicate copy of Hif-1α, except species in the cyprinid lineage that retained both paralogues of Hif-1α (Hif1aa and Hif1ab). Little is known about the contribution of Hif-1α, and specifically of each paralogue, to hypoxia tolerance. Here, we examined hypoxia tolerance in wild-type (Hif1aa
ab
) and Hif-1α knockout lines (Hif1aa
; Hif1ab
; Hif1aa
ab
) of zebrafish (
). Critical O
tension (
; the partial pressure of oxygen (PO
) at which O
consumption can no longer be maintained) and time to loss of equilibrium (LOE), two indices of hypoxia tolerance, were assessed in larvae and adults. Knockout of both paralogues significantly increased
(decreased hypoxia tolerance) in larval fish. Prior exposure of larvae to hypoxia decreased
in wild-type fish, an effect mediated by the Hif1aa paralogue. In adults, individuals with a knockout of either paralogue exhibited significantly decreased time to LOE but no difference in
. Together, these results demonstrate that in zebrafish, tolerance to hypoxia and improved hypoxia tolerance after pre-exposure to hypoxia (pre-conditioning) are mediated, at least in part, by Hif-1α.</description><subject>Development and Physiology</subject><issn>0962-8452</issn><issn>1471-2954</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpVkctuFDEQRS1ERIaELcvIy2TREz_H9gYJhQQijcSGrK2a7jJj5GlP7O6I8Ff5Eb4p3cpDsKla1K1bj0PIR86WnDl7Xup-sxRMsCUzzr4hC64Mb4TT6i1ZMLcSjVVaHJL3tf5ijDlt9TtyKIXS0jm-IGmda6U50O39Pv-O0MS-G9u4SUgDtEMulP99oBACtkN9EdEhJyzQt0hjTxOUO0gU-o5CN6aB_sFNgRDrlp7SL9DHTAuWKZ4dk4MAqeKH53xEbq4uf1x8a9bfv15ffF43rZJqaEzQYKGTTksVjJxmo2CSAXfKScNQrqyd7jAiaB60VrpT1jiDDMAAmk4ekU9Pvvtxs8OuxX4okPy-xB2Ue58h-v8rfdz6n_nOG2GNXLHJ4PTZoOTbEevgd7G2mBL0mMfqhWJGcr4Ss3T5JG3L9MmC4XUMZ35G5GdEfkbkZ0RTw8m_y73KX5jIR0v6jvY</recordid><startdate>20200527</startdate><enddate>20200527</enddate><creator>Mandic, Milica</creator><creator>Best, Carol</creator><creator>Perry, Steve F</creator><general>The Royal Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9377-4173</orcidid></search><sort><creationdate>20200527</creationdate><title>Loss of hypoxia-inducible factor 1α affects hypoxia tolerance in larval and adult zebrafish ( Danio rerio )</title><author>Mandic, Milica ; Best, Carol ; Perry, Steve F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-7f5a8ad39534f73ffee2030a1949370e368858572f51f5545d48797e0aa7ae7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Development and Physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mandic, Milica</creatorcontrib><creatorcontrib>Best, Carol</creatorcontrib><creatorcontrib>Perry, Steve F</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the Royal Society. B, Biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mandic, Milica</au><au>Best, Carol</au><au>Perry, Steve F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Loss of hypoxia-inducible factor 1α affects hypoxia tolerance in larval and adult zebrafish ( Danio rerio )</atitle><jtitle>Proceedings of the Royal Society. B, Biological sciences</jtitle><addtitle>Proc Biol Sci</addtitle><date>2020-05-27</date><risdate>2020</risdate><volume>287</volume><issue>1927</issue><spage>20200798</spage><epage>20200798</epage><pages>20200798-20200798</pages><issn>0962-8452</issn><eissn>1471-2954</eissn><abstract>The coordination of the hypoxic response is attributed, in part, to hypoxia-inducible factor 1α (Hif-1α), a regulator of hypoxia-induced transcription. After the teleost-specific genome duplication, most teleost fishes lost the duplicate copy of Hif-1α, except species in the cyprinid lineage that retained both paralogues of Hif-1α (Hif1aa and Hif1ab). Little is known about the contribution of Hif-1α, and specifically of each paralogue, to hypoxia tolerance. Here, we examined hypoxia tolerance in wild-type (Hif1aa
ab
) and Hif-1α knockout lines (Hif1aa
; Hif1ab
; Hif1aa
ab
) of zebrafish (
). Critical O
tension (
; the partial pressure of oxygen (PO
) at which O
consumption can no longer be maintained) and time to loss of equilibrium (LOE), two indices of hypoxia tolerance, were assessed in larvae and adults. Knockout of both paralogues significantly increased
(decreased hypoxia tolerance) in larval fish. Prior exposure of larvae to hypoxia decreased
in wild-type fish, an effect mediated by the Hif1aa paralogue. In adults, individuals with a knockout of either paralogue exhibited significantly decreased time to LOE but no difference in
. Together, these results demonstrate that in zebrafish, tolerance to hypoxia and improved hypoxia tolerance after pre-exposure to hypoxia (pre-conditioning) are mediated, at least in part, by Hif-1α.</abstract><cop>England</cop><pub>The Royal Society</pub><pmid>32453991</pmid><doi>10.1098/rspb.2020.0798</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-9377-4173</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Development and Physiology |
title | Loss of hypoxia-inducible factor 1α affects hypoxia tolerance in larval and adult zebrafish ( Danio rerio ) |
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