p66shc siRNA-Encapsulated PLGA Nanoparticles Ameliorate Neuropathic Pain Following Spinal Nerve Ligation
p66shc, a member of the shc adaptor protein family, has been shown to participate in regulation of mitochondrial homeostasis, apoptosis, and autophagosome formation. The present study was performed to investigate whether p66shc siRNA-encapsulated poly(d,l-lactic- -glycolic acid) nanoparticles (p66sh...
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| Veröffentlicht in: | Polymers 2020-04, Vol.12 (5), p.1014 |
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| description | p66shc, a member of the shc adaptor protein family, has been shown to participate in regulation of mitochondrial homeostasis, apoptosis, and autophagosome formation. The present study was performed to investigate whether p66shc siRNA-encapsulated poly(d,l-lactic-
-glycolic acid) nanoparticles (p66shc siRNA-PLGA NPs) can attenuate spinal nerve ligation (SNL)-induced neuropathic pain in rats. The SNL-induced pain behavior was decreased in the p66shc siRNA-PLGA NP-treated group compared with the scrambled siRNA-PLGA NP-treated group. In the L5 spinal cord of the p66shc siRNA-PLGA NP-treated group, expression levels of phosphorylated p66shc, cleaved caspase-3, p62, and PINK1, as well as microglial activation, were also decreased. In addition, p66shc knockdown using p66shc siRNA reduced the expression levels of cleaved caspase-3, p62, and PINK1, as well as proinflammatory mediators in the H
O
-treated HT22 neuronal cells. These results suggest that downregulation of p66shc expression in the spinal cord using p66shc siRNA-PLGA NPs could reduce the SNL-induced neuropathic pain by attenuating the SNL-induced aberrant autophagic, mitophagic, and neuroinflammatory processes in rats. |
| doi_str_mv | 10.3390/polym12051014 |
| format | Article |
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-glycolic acid) nanoparticles (p66shc siRNA-PLGA NPs) can attenuate spinal nerve ligation (SNL)-induced neuropathic pain in rats. The SNL-induced pain behavior was decreased in the p66shc siRNA-PLGA NP-treated group compared with the scrambled siRNA-PLGA NP-treated group. In the L5 spinal cord of the p66shc siRNA-PLGA NP-treated group, expression levels of phosphorylated p66shc, cleaved caspase-3, p62, and PINK1, as well as microglial activation, were also decreased. In addition, p66shc knockdown using p66shc siRNA reduced the expression levels of cleaved caspase-3, p62, and PINK1, as well as proinflammatory mediators in the H
O
-treated HT22 neuronal cells. These results suggest that downregulation of p66shc expression in the spinal cord using p66shc siRNA-PLGA NPs could reduce the SNL-induced neuropathic pain by attenuating the SNL-induced aberrant autophagic, mitophagic, and neuroinflammatory processes in rats.</description><identifier>ISSN: 2073-4360</identifier><identifier>EISSN: 2073-4360</identifier><identifier>DOI: 10.3390/polym12051014</identifier><identifier>PMID: 32365512</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Apoptosis ; Autophagy ; Cytochrome ; Efficiency ; Encapsulation ; Glycolic acid ; Homeostasis ; Hydrogen peroxide ; Nanoparticles ; Oxidative stress ; Pain ; Polyvinyl alcohol ; Proteins ; Spinal cord ; Surgery</subject><ispartof>Polymers, 2020-04, Vol.12 (5), p.1014</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-913a13362dd576c6e2eb2e9f8a354bc902338c0d527dc0bc7c1a5d3071ac1e113</citedby><cites>FETCH-LOGICAL-c415t-913a13362dd576c6e2eb2e9f8a354bc902338c0d527dc0bc7c1a5d3071ac1e113</cites><orcidid>0000-0001-5508-9004 ; 0000-0001-7691-1394 ; 0000-0003-3122-9417 ; 0000-0002-3530-101X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284875/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284875/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32365512$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shin, Nara</creatorcontrib><creatorcontrib>Shin, Hyo Jung</creatorcontrib><creatorcontrib>Yi, Yoonyoung</creatorcontrib><creatorcontrib>Beom, Jaewon</creatorcontrib><creatorcontrib>Lee, Wonhyung</creatorcontrib><creatorcontrib>Lee, Choong-Hyun</creatorcontrib><creatorcontrib>Kim, Dong Woon</creatorcontrib><title>p66shc siRNA-Encapsulated PLGA Nanoparticles Ameliorate Neuropathic Pain Following Spinal Nerve Ligation</title><title>Polymers</title><addtitle>Polymers (Basel)</addtitle><description>p66shc, a member of the shc adaptor protein family, has been shown to participate in regulation of mitochondrial homeostasis, apoptosis, and autophagosome formation. The present study was performed to investigate whether p66shc siRNA-encapsulated poly(d,l-lactic-
-glycolic acid) nanoparticles (p66shc siRNA-PLGA NPs) can attenuate spinal nerve ligation (SNL)-induced neuropathic pain in rats. The SNL-induced pain behavior was decreased in the p66shc siRNA-PLGA NP-treated group compared with the scrambled siRNA-PLGA NP-treated group. In the L5 spinal cord of the p66shc siRNA-PLGA NP-treated group, expression levels of phosphorylated p66shc, cleaved caspase-3, p62, and PINK1, as well as microglial activation, were also decreased. In addition, p66shc knockdown using p66shc siRNA reduced the expression levels of cleaved caspase-3, p62, and PINK1, as well as proinflammatory mediators in the H
O
-treated HT22 neuronal cells. These results suggest that downregulation of p66shc expression in the spinal cord using p66shc siRNA-PLGA NPs could reduce the SNL-induced neuropathic pain by attenuating the SNL-induced aberrant autophagic, mitophagic, and neuroinflammatory processes in rats.</description><subject>Apoptosis</subject><subject>Autophagy</subject><subject>Cytochrome</subject><subject>Efficiency</subject><subject>Encapsulation</subject><subject>Glycolic acid</subject><subject>Homeostasis</subject><subject>Hydrogen peroxide</subject><subject>Nanoparticles</subject><subject>Oxidative stress</subject><subject>Pain</subject><subject>Polyvinyl alcohol</subject><subject>Proteins</subject><subject>Spinal cord</subject><subject>Surgery</subject><issn>2073-4360</issn><issn>2073-4360</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkUtLJDEUhcMwMoq6nO0QcDOb0iQ3j6rNQCO-oGnFmVmHdCrdHUklZVKl-O8tn6h3cy-cj8M9HIR-UnII0JCjPoWHjjIiKKH8G9phREHFQZLvH-5ttF_KDZmGCymp-oG2gYEUgrIdtOmlLBuLi79ezKqTaE1fxmAG1-Kr-dkML0xMvcmDt8EVPOtc8ClPMl64MU_KsPEWXxkf8WkKId37uMZ_ex9NmIh85_Dcr83gU9xDWysTitt_3bvo_-nJv-Pzan55dnE8m1eWUzFUDQVDASRrW6GklY65JXPNqjYg-NI2hAHUlrSCqdaSpVWWGtECUdRY6iiFXfTnxbcfl51rrYtDNkH32XcmP-hkvP6sRL_R63SnFat5rcRk8PvVIKfb0ZVBd75YF4KJLo1FM2hqCRIYn9CDL-hNGvOU_ZlSnFP5bFi9UDanUrJbvT9DiX6qUX-qceJ_fUzwTr-VBo-c2pl2</recordid><startdate>20200429</startdate><enddate>20200429</enddate><creator>Shin, Nara</creator><creator>Shin, Hyo Jung</creator><creator>Yi, Yoonyoung</creator><creator>Beom, Jaewon</creator><creator>Lee, Wonhyung</creator><creator>Lee, Choong-Hyun</creator><creator>Kim, Dong Woon</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5508-9004</orcidid><orcidid>https://orcid.org/0000-0001-7691-1394</orcidid><orcidid>https://orcid.org/0000-0003-3122-9417</orcidid><orcidid>https://orcid.org/0000-0002-3530-101X</orcidid></search><sort><creationdate>20200429</creationdate><title>p66shc siRNA-Encapsulated PLGA Nanoparticles Ameliorate Neuropathic Pain Following Spinal Nerve Ligation</title><author>Shin, Nara ; Shin, Hyo Jung ; Yi, Yoonyoung ; Beom, Jaewon ; Lee, Wonhyung ; Lee, Choong-Hyun ; Kim, Dong Woon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-913a13362dd576c6e2eb2e9f8a354bc902338c0d527dc0bc7c1a5d3071ac1e113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Apoptosis</topic><topic>Autophagy</topic><topic>Cytochrome</topic><topic>Efficiency</topic><topic>Encapsulation</topic><topic>Glycolic acid</topic><topic>Homeostasis</topic><topic>Hydrogen peroxide</topic><topic>Nanoparticles</topic><topic>Oxidative stress</topic><topic>Pain</topic><topic>Polyvinyl alcohol</topic><topic>Proteins</topic><topic>Spinal cord</topic><topic>Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shin, Nara</creatorcontrib><creatorcontrib>Shin, Hyo Jung</creatorcontrib><creatorcontrib>Yi, Yoonyoung</creatorcontrib><creatorcontrib>Beom, Jaewon</creatorcontrib><creatorcontrib>Lee, Wonhyung</creatorcontrib><creatorcontrib>Lee, Choong-Hyun</creatorcontrib><creatorcontrib>Kim, Dong Woon</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shin, Nara</au><au>Shin, Hyo Jung</au><au>Yi, Yoonyoung</au><au>Beom, Jaewon</au><au>Lee, Wonhyung</au><au>Lee, Choong-Hyun</au><au>Kim, Dong Woon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>p66shc siRNA-Encapsulated PLGA Nanoparticles Ameliorate Neuropathic Pain Following Spinal Nerve Ligation</atitle><jtitle>Polymers</jtitle><addtitle>Polymers (Basel)</addtitle><date>2020-04-29</date><risdate>2020</risdate><volume>12</volume><issue>5</issue><spage>1014</spage><pages>1014-</pages><issn>2073-4360</issn><eissn>2073-4360</eissn><abstract>p66shc, a member of the shc adaptor protein family, has been shown to participate in regulation of mitochondrial homeostasis, apoptosis, and autophagosome formation. The present study was performed to investigate whether p66shc siRNA-encapsulated poly(d,l-lactic-
-glycolic acid) nanoparticles (p66shc siRNA-PLGA NPs) can attenuate spinal nerve ligation (SNL)-induced neuropathic pain in rats. The SNL-induced pain behavior was decreased in the p66shc siRNA-PLGA NP-treated group compared with the scrambled siRNA-PLGA NP-treated group. In the L5 spinal cord of the p66shc siRNA-PLGA NP-treated group, expression levels of phosphorylated p66shc, cleaved caspase-3, p62, and PINK1, as well as microglial activation, were also decreased. In addition, p66shc knockdown using p66shc siRNA reduced the expression levels of cleaved caspase-3, p62, and PINK1, as well as proinflammatory mediators in the H
O
-treated HT22 neuronal cells. These results suggest that downregulation of p66shc expression in the spinal cord using p66shc siRNA-PLGA NPs could reduce the SNL-induced neuropathic pain by attenuating the SNL-induced aberrant autophagic, mitophagic, and neuroinflammatory processes in rats.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>32365512</pmid><doi>10.3390/polym12051014</doi><orcidid>https://orcid.org/0000-0001-5508-9004</orcidid><orcidid>https://orcid.org/0000-0001-7691-1394</orcidid><orcidid>https://orcid.org/0000-0003-3122-9417</orcidid><orcidid>https://orcid.org/0000-0002-3530-101X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Apoptosis Autophagy Cytochrome Efficiency Encapsulation Glycolic acid Homeostasis Hydrogen peroxide Nanoparticles Oxidative stress Pain Polyvinyl alcohol Proteins Spinal cord Surgery |
| title | p66shc siRNA-Encapsulated PLGA Nanoparticles Ameliorate Neuropathic Pain Following Spinal Nerve Ligation |
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