A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos

Further refinement of culture media is needed to improve the quality of embryos generated in vitro . Previous results from our laboratory demonstrated that uptake of nutrients by the embryo is significantly less than what is supplied in traditional culture media. Our objective was to determine the i...

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Veröffentlicht in:	Scientific reports 2020-06, Vol.10 (1), p.9263, Article 9263
Hauptverfasser:	Ermisch, Alison F., Herrick, Jason R., Pasquariello, Rolando, Dyer, McKenna C., Lyons, Sarah M., Broeckling, Corey D., Rajput, Sandeep K., Schoolcraft, William B., Krisher, Rebecca L.
Format:	Artikel
Sprache:	eng
Schlagworte:	13/106 38/77 45/90 631/136/1455 631/443/494/2732/1577 64/60 692/308/575 82/80 Amino acids Amino Acids, Essential - pharmacology Animals Blastocyst - cytology Blastocyst - drug effects Blastocyst - physiology Blastocysts Carbohydrates Cell culture Cell number Culture media Culture Media - chemistry Culture Media - pharmacology Dipeptides - pharmacology Edetic Acid - pharmacology Embryo Culture Techniques - methods Embryo Transfer Embryos Female Fertilization in Vitro Fetuses Gene Expression Regulation, Developmental - drug effects Glucose - pharmacology Hatching Humanities and Social Sciences Lactic acid Lactic Acid - pharmacology Metabolic pathways Metabolism Mice multidisciplinary Nutrient concentrations Nutrient requirements Nutrient uptake Nutrients Pyruvic acid Pyruvic Acid - pharmacology Science Science (multidisciplinary) Vitamins
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