A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos
Further refinement of culture media is needed to improve the quality of embryos generated in vitro . Previous results from our laboratory demonstrated that uptake of nutrients by the embryo is significantly less than what is supplied in traditional culture media. Our objective was to determine the i...
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| Veröffentlicht in: | Scientific reports 2020-06, Vol.10 (1), p.9263, Article 9263 |
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| creator | Ermisch, Alison F. Herrick, Jason R. Pasquariello, Rolando Dyer, McKenna C. Lyons, Sarah M. Broeckling, Corey D. Rajput, Sandeep K. Schoolcraft, William B. Krisher, Rebecca L. |
| description | Further refinement of culture media is needed to improve the quality of embryos generated
in vitro
. Previous results from our laboratory demonstrated that uptake of nutrients by the embryo is significantly less than what is supplied in traditional culture media. Our objective was to determine the impact of reduced nutrient concentrations in culture medium on mouse embryo development, metabolism, and quality as a possible platform for next generation medium formulation. Concentrations of carbohydrates, amino acids, and vitamins could be reduced by 50% with no detrimental effects, but blastocyst development was impaired at 25% of standard nutrient provision (reduced nutrient medium; RN). Addition of pyruvate and L-lactate (+PL) to RN at 50% of standard concentrations restored blastocyst development, hatching, and cell number. In addition, blastocysts produced in RN + PL contained more ICM cells and ATP than blastocysts cultured in our control (100% nutrient) medium; however, metabolic activity was altered. Similarly, embryos produced in the RN medium with elevated (50% control) concentrations of pyruvate and lactate in the first step medium and EAA and Glu in the second step medium were competent to implant and develop into fetuses at a similar rate as embryos produced in the control medium. This novel approach to culture medium formulation could help define the optimal nutrient requirements of embryos in culture and provide a means of shifting metabolic activity towards the utilization of specific metabolic pathways that may be beneficial for embryo viability. |
| doi_str_mv | 10.1038/s41598-020-66019-4 |
| format | Article |
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. Previous results from our laboratory demonstrated that uptake of nutrients by the embryo is significantly less than what is supplied in traditional culture media. Our objective was to determine the impact of reduced nutrient concentrations in culture medium on mouse embryo development, metabolism, and quality as a possible platform for next generation medium formulation. Concentrations of carbohydrates, amino acids, and vitamins could be reduced by 50% with no detrimental effects, but blastocyst development was impaired at 25% of standard nutrient provision (reduced nutrient medium; RN). Addition of pyruvate and L-lactate (+PL) to RN at 50% of standard concentrations restored blastocyst development, hatching, and cell number. In addition, blastocysts produced in RN + PL contained more ICM cells and ATP than blastocysts cultured in our control (100% nutrient) medium; however, metabolic activity was altered. Similarly, embryos produced in the RN medium with elevated (50% control) concentrations of pyruvate and lactate in the first step medium and EAA and Glu in the second step medium were competent to implant and develop into fetuses at a similar rate as embryos produced in the control medium. This novel approach to culture medium formulation could help define the optimal nutrient requirements of embryos in culture and provide a means of shifting metabolic activity towards the utilization of specific metabolic pathways that may be beneficial for embryo viability.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-66019-4</identifier><identifier>PMID: 32518371</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/106 ; 38/77 ; 45/90 ; 631/136/1455 ; 631/443/494/2732/1577 ; 64/60 ; 692/308/575 ; 82/80 ; Amino acids ; Amino Acids, Essential - pharmacology ; Animals ; Blastocyst - cytology ; Blastocyst - drug effects ; Blastocyst - physiology ; Blastocysts ; Carbohydrates ; Cell culture ; Cell number ; Culture media ; Culture Media - chemistry ; Culture Media - pharmacology ; Dipeptides - pharmacology ; Edetic Acid - pharmacology ; Embryo Culture Techniques - methods ; Embryo Transfer ; Embryos ; Female ; Fertilization in Vitro ; Fetuses ; Gene Expression Regulation, Developmental - drug effects ; Glucose - pharmacology ; Hatching ; Humanities and Social Sciences ; Lactic acid ; Lactic Acid - pharmacology ; Metabolic pathways ; Metabolism ; Mice ; multidisciplinary ; Nutrient concentrations ; Nutrient requirements ; Nutrient uptake ; Nutrients ; Pyruvic acid ; Pyruvic Acid - pharmacology ; Science ; Science (multidisciplinary) ; Vitamins</subject><ispartof>Scientific reports, 2020-06, Vol.10 (1), p.9263, Article 9263</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-3b8106f1f8b4665bffb6f6fb6825f277208fec4f9b9d49357fb5d8f4a6ce35b43</citedby><cites>FETCH-LOGICAL-c511t-3b8106f1f8b4665bffb6f6fb6825f277208fec4f9b9d49357fb5d8f4a6ce35b43</cites><orcidid>0000-0003-3027-9324 ; 0000-0003-3191-7009 ; 0000-0002-6158-827X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283311/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283311/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27922,27923,41118,42187,51574,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32518371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ermisch, Alison F.</creatorcontrib><creatorcontrib>Herrick, Jason R.</creatorcontrib><creatorcontrib>Pasquariello, Rolando</creatorcontrib><creatorcontrib>Dyer, McKenna C.</creatorcontrib><creatorcontrib>Lyons, Sarah M.</creatorcontrib><creatorcontrib>Broeckling, Corey D.</creatorcontrib><creatorcontrib>Rajput, Sandeep K.</creatorcontrib><creatorcontrib>Schoolcraft, William B.</creatorcontrib><creatorcontrib>Krisher, Rebecca L.</creatorcontrib><title>A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Further refinement of culture media is needed to improve the quality of embryos generated
in vitro
. Previous results from our laboratory demonstrated that uptake of nutrients by the embryo is significantly less than what is supplied in traditional culture media. Our objective was to determine the impact of reduced nutrient concentrations in culture medium on mouse embryo development, metabolism, and quality as a possible platform for next generation medium formulation. Concentrations of carbohydrates, amino acids, and vitamins could be reduced by 50% with no detrimental effects, but blastocyst development was impaired at 25% of standard nutrient provision (reduced nutrient medium; RN). Addition of pyruvate and L-lactate (+PL) to RN at 50% of standard concentrations restored blastocyst development, hatching, and cell number. In addition, blastocysts produced in RN + PL contained more ICM cells and ATP than blastocysts cultured in our control (100% nutrient) medium; however, metabolic activity was altered. Similarly, embryos produced in the RN medium with elevated (50% control) concentrations of pyruvate and lactate in the first step medium and EAA and Glu in the second step medium were competent to implant and develop into fetuses at a similar rate as embryos produced in the control medium. This novel approach to culture medium formulation could help define the optimal nutrient requirements of embryos in culture and provide a means of shifting metabolic activity towards the utilization of specific metabolic pathways that may be beneficial for embryo viability.</description><subject>13/106</subject><subject>38/77</subject><subject>45/90</subject><subject>631/136/1455</subject><subject>631/443/494/2732/1577</subject><subject>64/60</subject><subject>692/308/575</subject><subject>82/80</subject><subject>Amino acids</subject><subject>Amino Acids, Essential - pharmacology</subject><subject>Animals</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - drug effects</subject><subject>Blastocyst - physiology</subject><subject>Blastocysts</subject><subject>Carbohydrates</subject><subject>Cell culture</subject><subject>Cell number</subject><subject>Culture media</subject><subject>Culture Media - chemistry</subject><subject>Culture Media - pharmacology</subject><subject>Dipeptides - 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in vitro
. Previous results from our laboratory demonstrated that uptake of nutrients by the embryo is significantly less than what is supplied in traditional culture media. Our objective was to determine the impact of reduced nutrient concentrations in culture medium on mouse embryo development, metabolism, and quality as a possible platform for next generation medium formulation. Concentrations of carbohydrates, amino acids, and vitamins could be reduced by 50% with no detrimental effects, but blastocyst development was impaired at 25% of standard nutrient provision (reduced nutrient medium; RN). Addition of pyruvate and L-lactate (+PL) to RN at 50% of standard concentrations restored blastocyst development, hatching, and cell number. In addition, blastocysts produced in RN + PL contained more ICM cells and ATP than blastocysts cultured in our control (100% nutrient) medium; however, metabolic activity was altered. Similarly, embryos produced in the RN medium with elevated (50% control) concentrations of pyruvate and lactate in the first step medium and EAA and Glu in the second step medium were competent to implant and develop into fetuses at a similar rate as embryos produced in the control medium. This novel approach to culture medium formulation could help define the optimal nutrient requirements of embryos in culture and provide a means of shifting metabolic activity towards the utilization of specific metabolic pathways that may be beneficial for embryo viability.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32518371</pmid><doi>10.1038/s41598-020-66019-4</doi><orcidid>https://orcid.org/0000-0003-3027-9324</orcidid><orcidid>https://orcid.org/0000-0003-3191-7009</orcidid><orcidid>https://orcid.org/0000-0002-6158-827X</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Scientific reports, 2020-06, Vol.10 (1), p.9263, Article 9263 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 13/106 38/77 45/90 631/136/1455 631/443/494/2732/1577 64/60 692/308/575 82/80 Amino acids Amino Acids, Essential - pharmacology Animals Blastocyst - cytology Blastocyst - drug effects Blastocyst - physiology Blastocysts Carbohydrates Cell culture Cell number Culture media Culture Media - chemistry Culture Media - pharmacology Dipeptides - pharmacology Edetic Acid - pharmacology Embryo Culture Techniques - methods Embryo Transfer Embryos Female Fertilization in Vitro Fetuses Gene Expression Regulation, Developmental - drug effects Glucose - pharmacology Hatching Humanities and Social Sciences Lactic acid Lactic Acid - pharmacology Metabolic pathways Metabolism Mice multidisciplinary Nutrient concentrations Nutrient requirements Nutrient uptake Nutrients Pyruvic acid Pyruvic Acid - pharmacology Science Science (multidisciplinary) Vitamins |
| title | A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos |
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