Biodegradation of Bisphenol A by Sphingobium sp. YC-JY1 and the Essential Role of Cytochrome P450 Monooxygenase
Bisphenol A (BPA) is a widespread pollutant threatening the ecosystem and human health. An effective BPA degrader YC-JY1 was isolated and identified as sp. The optimal temperature and pH for the degradation of BPA by strain YC-JY1 were 30 °C and 6.5, respectively. The biodegradation pathway was prop...
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| Veröffentlicht in: | International journal of molecular sciences 2020-05, Vol.21 (10), p.3588 |
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| creator | Jia, Yang Eltoukhy, Adel Wang, Junhuan Li, Xianjun Hlaing, Thet Su Aung, Mar Mar Nwe, May Thet Lamraoui, Imane Yan, Yanchun |
| description | Bisphenol A (BPA) is a widespread pollutant threatening the ecosystem and human health. An effective BPA degrader YC-JY1 was isolated and identified as
sp. The optimal temperature and pH for the degradation of BPA by strain YC-JY1 were 30 °C and 6.5, respectively. The biodegradation pathway was proposed based on the identification of the metabolites. The addition of cytochrome P450 (CYP) inhibitor 1-aminobenzotriazole significantly decreased the degradation of BPA by
sp. YC-JY1.
BL21 (DE3) cells harboring pET28a-
achieved the ability to degrade BPA. The
gene knockout strain YC-JY1Δ
was unable to degrade BPA indicating that P450
was an essential initiator of BPA metabolism in strain YC-JY1. For BPA polluted soil remediation, strain YC-JY1 considerably stimulated biodegradation of BPA associated with the soil microbial community. These results point out that strain YC-JY1 is a promising microbe for BPA removal and possesses great application potential. |
| doi_str_mv | 10.3390/ijms21103588 |
| format | Article |
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sp. The optimal temperature and pH for the degradation of BPA by strain YC-JY1 were 30 °C and 6.5, respectively. The biodegradation pathway was proposed based on the identification of the metabolites. The addition of cytochrome P450 (CYP) inhibitor 1-aminobenzotriazole significantly decreased the degradation of BPA by
sp. YC-JY1.
BL21 (DE3) cells harboring pET28a-
achieved the ability to degrade BPA. The
gene knockout strain YC-JY1Δ
was unable to degrade BPA indicating that P450
was an essential initiator of BPA metabolism in strain YC-JY1. For BPA polluted soil remediation, strain YC-JY1 considerably stimulated biodegradation of BPA associated with the soil microbial community. These results point out that strain YC-JY1 is a promising microbe for BPA removal and possesses great application potential.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms21103588</identifier><identifier>PMID: 32438730</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Bacteria ; Benzhydryl Compounds - metabolism ; Biodegradation ; Biodegradation, Environmental ; Bisphenol A ; Carbon ; Cytochrome ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P-450 Enzyme System - metabolism ; Cytochrome P450 ; Cytochrome P450 monooxygenase ; Cytochromes P450 ; Efficiency ; Enzymes ; Genes ; Hydrogen-Ion Concentration ; Metabolism ; Metabolites ; Metabolome ; Microorganisms ; Phenols - metabolism ; Phylogenetics ; Phylogeny ; Pollutants ; Retention ; Soil microorganisms ; Soil Pollutants ; Soil pollution ; Soil remediation ; Sphingobium ; Sphingomonadaceae - enzymology ; Sphingomonadaceae - genetics ; Temperature</subject><ispartof>International journal of molecular sciences, 2020-05, Vol.21 (10), p.3588</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-b256048b7ce67e67d8d6b082692963adfc0a0a2dd1b2beaf5fd8bb5c10f245963</citedby><cites>FETCH-LOGICAL-c412t-b256048b7ce67e67d8d6b082692963adfc0a0a2dd1b2beaf5fd8bb5c10f245963</cites><orcidid>0000-0002-9590-7646</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278973/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278973/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32438730$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jia, Yang</creatorcontrib><creatorcontrib>Eltoukhy, Adel</creatorcontrib><creatorcontrib>Wang, Junhuan</creatorcontrib><creatorcontrib>Li, Xianjun</creatorcontrib><creatorcontrib>Hlaing, Thet Su</creatorcontrib><creatorcontrib>Aung, Mar Mar</creatorcontrib><creatorcontrib>Nwe, May Thet</creatorcontrib><creatorcontrib>Lamraoui, Imane</creatorcontrib><creatorcontrib>Yan, Yanchun</creatorcontrib><title>Biodegradation of Bisphenol A by Sphingobium sp. YC-JY1 and the Essential Role of Cytochrome P450 Monooxygenase</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Bisphenol A (BPA) is a widespread pollutant threatening the ecosystem and human health. An effective BPA degrader YC-JY1 was isolated and identified as
sp. The optimal temperature and pH for the degradation of BPA by strain YC-JY1 were 30 °C and 6.5, respectively. The biodegradation pathway was proposed based on the identification of the metabolites. The addition of cytochrome P450 (CYP) inhibitor 1-aminobenzotriazole significantly decreased the degradation of BPA by
sp. YC-JY1.
BL21 (DE3) cells harboring pET28a-
achieved the ability to degrade BPA. The
gene knockout strain YC-JY1Δ
was unable to degrade BPA indicating that P450
was an essential initiator of BPA metabolism in strain YC-JY1. For BPA polluted soil remediation, strain YC-JY1 considerably stimulated biodegradation of BPA associated with the soil microbial community. These results point out that strain YC-JY1 is a promising microbe for BPA removal and possesses great application potential.</description><subject>Bacteria</subject><subject>Benzhydryl Compounds - metabolism</subject><subject>Biodegradation</subject><subject>Biodegradation, Environmental</subject><subject>Bisphenol A</subject><subject>Carbon</subject><subject>Cytochrome</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Cytochrome P450</subject><subject>Cytochrome P450 monooxygenase</subject><subject>Cytochromes P450</subject><subject>Efficiency</subject><subject>Enzymes</subject><subject>Genes</subject><subject>Hydrogen-Ion Concentration</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolome</subject><subject>Microorganisms</subject><subject>Phenols - metabolism</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Pollutants</subject><subject>Retention</subject><subject>Soil microorganisms</subject><subject>Soil Pollutants</subject><subject>Soil pollution</subject><subject>Soil remediation</subject><subject>Sphingobium</subject><subject>Sphingomonadaceae - enzymology</subject><subject>Sphingomonadaceae - genetics</subject><subject>Temperature</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpVkctr3DAQxkVp6CZpbz0XQa91MpL8vBSSJU8SUvo47ElIlrzWYmscyRu6_30cslk2MDAD85tvPvgI-crgRIgKTt2qj5wxEFlZfiCHLOU8AciLj3vzjBzFuALggmfVJzITPBVlIeCQ4LlDY5dBGTU69BQbeu7i0FqPHT2jekP_DK3zS9Ru3dM4nNDFPLldMKq8oWNr6UWM1o9OdfQ3dvblfr4ZsW4D9pb-SjOg9-gR_2-W1qtoP5ODRnXRftn2Y_Lv8uLv_Dq5e7i6mZ_dJXXK-JhonuWQlrqobV5MZUqTayh5XvEqF8o0NShQ3BimubaqyRpTap3VDBqeZhNyTH6-6g5r3VtTTx6D6uQQXK_CRqJy8v3Gu1Yu8UkWvCirQkwC37cCAR_XNo5yhevgJ8-Sp5BzISBPJ-rHK1UHjDHYZveBgXyJR-7HM-Hf9l3t4Lc8xDN-oowj</recordid><startdate>20200519</startdate><enddate>20200519</enddate><creator>Jia, Yang</creator><creator>Eltoukhy, Adel</creator><creator>Wang, Junhuan</creator><creator>Li, Xianjun</creator><creator>Hlaing, Thet Su</creator><creator>Aung, Mar Mar</creator><creator>Nwe, May Thet</creator><creator>Lamraoui, Imane</creator><creator>Yan, Yanchun</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9590-7646</orcidid></search><sort><creationdate>20200519</creationdate><title>Biodegradation of Bisphenol A by Sphingobium sp. 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YC-JY1 and the Essential Role of Cytochrome P450 Monooxygenase</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2020-05-19</date><risdate>2020</risdate><volume>21</volume><issue>10</issue><spage>3588</spage><pages>3588-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Bisphenol A (BPA) is a widespread pollutant threatening the ecosystem and human health. An effective BPA degrader YC-JY1 was isolated and identified as
sp. The optimal temperature and pH for the degradation of BPA by strain YC-JY1 were 30 °C and 6.5, respectively. The biodegradation pathway was proposed based on the identification of the metabolites. The addition of cytochrome P450 (CYP) inhibitor 1-aminobenzotriazole significantly decreased the degradation of BPA by
sp. YC-JY1.
BL21 (DE3) cells harboring pET28a-
achieved the ability to degrade BPA. The
gene knockout strain YC-JY1Δ
was unable to degrade BPA indicating that P450
was an essential initiator of BPA metabolism in strain YC-JY1. For BPA polluted soil remediation, strain YC-JY1 considerably stimulated biodegradation of BPA associated with the soil microbial community. These results point out that strain YC-JY1 is a promising microbe for BPA removal and possesses great application potential.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>32438730</pmid><doi>10.3390/ijms21103588</doi><orcidid>https://orcid.org/0000-0002-9590-7646</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Bacteria Benzhydryl Compounds - metabolism Biodegradation Biodegradation, Environmental Bisphenol A Carbon Cytochrome Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Cytochrome P450 Cytochrome P450 monooxygenase Cytochromes P450 Efficiency Enzymes Genes Hydrogen-Ion Concentration Metabolism Metabolites Metabolome Microorganisms Phenols - metabolism Phylogenetics Phylogeny Pollutants Retention Soil microorganisms Soil Pollutants Soil pollution Soil remediation Sphingobium Sphingomonadaceae - enzymology Sphingomonadaceae - genetics Temperature |
| title | Biodegradation of Bisphenol A by Sphingobium sp. YC-JY1 and the Essential Role of Cytochrome P450 Monooxygenase |
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