Quantification of native mRNA dynamics in living neurons using fluorescence correlation spectroscopy and reduction-triggered fluorescent probes

RNA localization in subcellular compartments is essential for spatial and temporal regulation of protein expression in neurons. Several techniques have been developed to visualize mRNAs inside cells, but the study of the behavior of endogenous and nonengineered mRNAs in living neurons has just start...

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Veröffentlicht in:The Journal of biological chemistry 2020-06, Vol.295 (23), p.7923-7940
Hauptverfasser: Fujita, Hirotaka, Oikawa, Ryota, Hayakawa, Mayu, Tomoike, Fumiaki, Kimura, Yasuaki, Okuno, Hiroyuki, Hatashita, Yoshiki, Fiallos Oliveros, Carolina, Bito, Haruhiko, Ohshima, Toshio, Tsuneda, Satoshi, Abe, Hiroshi, Inoue, Takafumi
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Sprache:eng
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