A comparison of microfluidic methods for high-throughput cell deformability measurements
The mechanical phenotype of a cell is an inherent biophysical marker of its state and function, with many applications in basic and applied biological research. Microfluidics-based methods have enabled single-cell mechanophenotyping at throughputs comparable to those of flow cytometry. Here, we pres...
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| Veröffentlicht in: | Nature methods 2020-06, Vol.17 (6), p.587-593 |
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| description | The mechanical phenotype of a cell is an inherent biophysical marker of its state and function, with many applications in basic and applied biological research. Microfluidics-based methods have enabled single-cell mechanophenotyping at throughputs comparable to those of flow cytometry. Here, we present a standardized cross-laboratory study comparing three microfluidics-based approaches for measuring cell mechanical phenotype: constriction-based deformability cytometry (cDC), shear flow deformability cytometry (sDC) and extensional flow deformability cytometry (xDC). All three methods detect cell deformability changes induced by exposure to altered osmolarity. However, a dose-dependent deformability increase upon latrunculin B-induced actin disassembly was detected only with cDC and sDC, which suggests that when exposing cells to the higher strain rate imposed by xDC, cellular components other than the actin cytoskeleton dominate the response. The direct comparison presented here furthers our understanding of the applicability of the different deformability cytometry methods and provides context for the interpretation of deformability measurements performed using different platforms.
This Analysis compares microfluidics-based methods for assessing mechanical properties of cells in high throughput. |
| doi_str_mv | 10.1038/s41592-020-0818-8 |
| format | Article |
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Josephine</au><au>Otto, Oliver</au><au>Manalis, Scott R.</au><au>Di Carlo, Dino</au><au>Guck, Jochen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison of microfluidic methods for high-throughput cell deformability measurements</atitle><jtitle>Nature methods</jtitle><stitle>Nat Methods</stitle><addtitle>Nat Methods</addtitle><date>2020-06-01</date><risdate>2020</risdate><volume>17</volume><issue>6</issue><spage>587</spage><epage>593</epage><pages>587-593</pages><issn>1548-7091</issn><eissn>1548-7105</eissn><abstract>The mechanical phenotype of a cell is an inherent biophysical marker of its state and function, with many applications in basic and applied biological research. Microfluidics-based methods have enabled single-cell mechanophenotyping at throughputs comparable to those of flow cytometry. Here, we present a standardized cross-laboratory study comparing three microfluidics-based approaches for measuring cell mechanical phenotype: constriction-based deformability cytometry (cDC), shear flow deformability cytometry (sDC) and extensional flow deformability cytometry (xDC). All three methods detect cell deformability changes induced by exposure to altered osmolarity. However, a dose-dependent deformability increase upon latrunculin B-induced actin disassembly was detected only with cDC and sDC, which suggests that when exposing cells to the higher strain rate imposed by xDC, cellular components other than the actin cytoskeleton dominate the response. The direct comparison presented here furthers our understanding of the applicability of the different deformability cytometry methods and provides context for the interpretation of deformability measurements performed using different platforms.
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| subjects | 631/1647/2204 631/1647/277 Actin Actins - drug effects Analysis Bioengineering Bioinformatics Biological Microscopy Biological research Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Bridged Bicyclo Compounds, Heterocyclic - administration & dosage Cancer Cell cycle Cell Shape - drug effects Cell Size - drug effects Change detection Comparative analysis Cytoskeleton Cytoskeleton - drug effects Deformability Deformation Dose-Response Relationship, Drug Flow cytometry Flow Cytometry - methods Flow velocity Formability Genotype & phenotype HL-60 Cells Humans Image Processing, Computer-Assisted Latrunculin B Life Sciences Mechanical properties Mechanics Medical laboratories Medical research Methods Microfluidics Microfluidics - methods Muscle proteins Osmolarity Phenotypes Proteomics Reynolds number Shear flow Strain rate Thiazolidines - administration & dosage Viscosity |
| title | A comparison of microfluidic methods for high-throughput cell deformability measurements |
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