Imaging flowers: a guide to current microscopy and tomography techniques to study flower development
We review modern light microscopy and computed projection tomography methods, their capabilities and limitations, and their current and potential applications to the study of flower development and fertilization. Abstract Developmental biology relies heavily on our ability to generate three-dimensio...
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| Veröffentlicht in: | Journal of experimental botany 2020-05, Vol.71 (10), p.2898-2909 |
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| container_title | Journal of experimental botany |
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| creator | Prunet, Nathanaël Duncan, Keith |
| description | We review modern light microscopy and computed projection tomography methods, their capabilities and limitations, and their current and potential applications to the study of flower development and fertilization.
Abstract
Developmental biology relies heavily on our ability to generate three-dimensional images of live biological specimens through time, and to map gene expression and hormone response in these specimens as they undergo development. The last two decades have seen an explosion of new bioimaging technologies that have pushed the limits of spatial and temporal resolution and provided biologists with invaluable new tools. However, plant tissues are difficult to image, and no single technology fits all purposes; choosing between many bioimaging techniques is not trivial. Here, we review modern light microscopy and computed projection tomography methods, their capabilities and limitations, and we discuss their current and potential applications to the study of flower development and fertilization. |
| doi_str_mv | 10.1093/jxb/eraa094 |
| format | Article |
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Abstract
Developmental biology relies heavily on our ability to generate three-dimensional images of live biological specimens through time, and to map gene expression and hormone response in these specimens as they undergo development. The last two decades have seen an explosion of new bioimaging technologies that have pushed the limits of spatial and temporal resolution and provided biologists with invaluable new tools. However, plant tissues are difficult to image, and no single technology fits all purposes; choosing between many bioimaging techniques is not trivial. Here, we review modern light microscopy and computed projection tomography methods, their capabilities and limitations, and we discuss their current and potential applications to the study of flower development and fertilization.</description><identifier>ISSN: 0022-0957</identifier><identifier>EISSN: 1460-2431</identifier><identifier>DOI: 10.1093/jxb/eraa094</identifier><identifier>PMID: 32383442</identifier><language>eng</language><publisher>UK: Oxford University Press</publisher><subject>Flowering letter Reviews ; Flowers ; Imaging, Three-Dimensional ; Life Sciences & Biomedicine ; Microscopy ; Plant Sciences ; Science & Technology ; Tomography</subject><ispartof>Journal of experimental botany, 2020-05, Vol.71 (10), p.2898-2909</ispartof><rights>The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com 2020</rights><rights>The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>21</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000538790700008</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c478t-669d56aa05691568acfae4cf9370cbd9fb5465c8f0545b3fba83847a208d30e3</citedby><cites>FETCH-LOGICAL-c478t-669d56aa05691568acfae4cf9370cbd9fb5465c8f0545b3fba83847a208d30e3</cites><orcidid>0000-0002-8939-5920</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,781,785,886,1585,27929,27930,28253</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32383442$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wellmer, Frank</contributor><creatorcontrib>Prunet, Nathanaël</creatorcontrib><creatorcontrib>Duncan, Keith</creatorcontrib><title>Imaging flowers: a guide to current microscopy and tomography techniques to study flower development</title><title>Journal of experimental botany</title><addtitle>J EXP BOT</addtitle><addtitle>J Exp Bot</addtitle><description>We review modern light microscopy and computed projection tomography methods, their capabilities and limitations, and their current and potential applications to the study of flower development and fertilization.
Abstract
Developmental biology relies heavily on our ability to generate three-dimensional images of live biological specimens through time, and to map gene expression and hormone response in these specimens as they undergo development. The last two decades have seen an explosion of new bioimaging technologies that have pushed the limits of spatial and temporal resolution and provided biologists with invaluable new tools. However, plant tissues are difficult to image, and no single technology fits all purposes; choosing between many bioimaging techniques is not trivial. Here, we review modern light microscopy and computed projection tomography methods, their capabilities and limitations, and we discuss their current and potential applications to the study of flower development and fertilization.</description><subject>Flowering letter Reviews</subject><subject>Flowers</subject><subject>Imaging, Three-Dimensional</subject><subject>Life Sciences & Biomedicine</subject><subject>Microscopy</subject><subject>Plant Sciences</subject><subject>Science & Technology</subject><subject>Tomography</subject><issn>0022-0957</issn><issn>1460-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>TOX</sourceid><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><recordid>eNqNkctv1DAQxi0EokvhxB3lhJBQ6CS2E4cDElrxqFSpl94tx4-sq8QOttOy_z2OdrvApeppRprffPP4EHpbwacKOnxx-7u_0EEI6MgztKlIA2VNcPUcbQDquoSOtmfoVYy3AECB0pfoDNeYYULqDVKXkxisGwoz-nsd4udCFMNilS6SL-QSgnapmKwMPko_7wvhVK5Mfghi3u2LpOXO2V-Ljisf06L2R6VC6Ts9-nnKAq_RCyPGqN8c4zm6-f7tZvuzvLr-cbn9elVK0rJUNk2naJMPoU1X0YYJaYQm0nS4BdmrzvSUNFQyA5TQHpteMMxIK2pgCoPG5-jLQXZe-kkrmScHMfI52EmEPffC8v8rzu744O94WzfQVpAFPhwFgl9vSnyyUepxFE77JfKa5A8SVtEuox8P6PqZGLQ5jamAr7bwbAs_2pLpd_9udmIffPgrd697b6K02kl9wlbjMGs7aHMGLNPs6fTWJpGsd1u_uJRb3x9a_TI_uvIfQ4-6yg</recordid><startdate>20200530</startdate><enddate>20200530</enddate><creator>Prunet, Nathanaël</creator><creator>Duncan, Keith</creator><general>Oxford University Press</general><general>Oxford Univ Press</general><scope>TOX</scope><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8939-5920</orcidid></search><sort><creationdate>20200530</creationdate><title>Imaging flowers: a guide to current microscopy and tomography techniques to study flower development</title><author>Prunet, Nathanaël ; Duncan, Keith</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-669d56aa05691568acfae4cf9370cbd9fb5465c8f0545b3fba83847a208d30e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Flowering letter Reviews</topic><topic>Flowers</topic><topic>Imaging, Three-Dimensional</topic><topic>Life Sciences & Biomedicine</topic><topic>Microscopy</topic><topic>Plant Sciences</topic><topic>Science & Technology</topic><topic>Tomography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prunet, Nathanaël</creatorcontrib><creatorcontrib>Duncan, Keith</creatorcontrib><collection>Access via Oxford University Press (Open Access Collection)</collection><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of experimental botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prunet, Nathanaël</au><au>Duncan, Keith</au><au>Wellmer, Frank</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Imaging flowers: a guide to current microscopy and tomography techniques to study flower development</atitle><jtitle>Journal of experimental botany</jtitle><stitle>J EXP BOT</stitle><addtitle>J Exp Bot</addtitle><date>2020-05-30</date><risdate>2020</risdate><volume>71</volume><issue>10</issue><spage>2898</spage><epage>2909</epage><pages>2898-2909</pages><issn>0022-0957</issn><eissn>1460-2431</eissn><abstract>We review modern light microscopy and computed projection tomography methods, their capabilities and limitations, and their current and potential applications to the study of flower development and fertilization.
Abstract
Developmental biology relies heavily on our ability to generate three-dimensional images of live biological specimens through time, and to map gene expression and hormone response in these specimens as they undergo development. The last two decades have seen an explosion of new bioimaging technologies that have pushed the limits of spatial and temporal resolution and provided biologists with invaluable new tools. However, plant tissues are difficult to image, and no single technology fits all purposes; choosing between many bioimaging techniques is not trivial. Here, we review modern light microscopy and computed projection tomography methods, their capabilities and limitations, and we discuss their current and potential applications to the study of flower development and fertilization.</abstract><cop>UK</cop><pub>Oxford University Press</pub><pmid>32383442</pmid><doi>10.1093/jxb/eraa094</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-8939-5920</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Web of Science - Science Citation Index Expanded - 2020<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Flowering letter Reviews Flowers Imaging, Three-Dimensional Life Sciences & Biomedicine Microscopy Plant Sciences Science & Technology Tomography |
| title | Imaging flowers: a guide to current microscopy and tomography techniques to study flower development |
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