Evaluation of C-C Chemokine Ligand 5 (CCL5) Chemokine, Interleukin 5 (IL-5) Cytokine, and Eosinophil Counts as Potential Biomarkers in Saudi Patients with Chronic Asthma During Sandstorms
Background and objectives Asthma is a common chronic inflammatory disorder of the lung that can be exacerbated by environmental triggers during sandstorms. This study aimed to evaluate the usefulness of C-C chemokine ligand 5 (CCL5) chemokine and interleukin 5 (IL-5) cytokine and determine the total...
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description | Background and objectives Asthma is a common chronic inflammatory disorder of the lung that can be exacerbated by environmental triggers during sandstorms. This study aimed to evaluate the usefulness of C-C chemokine ligand 5 (CCL5) chemokine and interleukin 5 (IL-5) cytokine and determine the total eosinophil count in blood and sputum for use as biomarkers in Saudi patients with chronic asthma who visited emergency departments during sandstorms. Methods The study included 42 Saudi patients with chronic asthma and 20 healthy controls. Plasma levels of CCL5, IL-5, and total immunoglobulin E (IgE) were measured using a specific enzyme-linked immunosorbent assay (ELISA). Total eosinophils in peripheral blood were counted using a hematology analyzer (CELL-DYN Ruby System; Abbott Diagnostics, Chicago, Illinois); in sputum, eosinophils stained with Giemsa were examined under a microscope, counted, and expressed as a percentage of the total cells. Results Total IgE levels were significantly higher in patients with asthma (mean 433 IU/ml, P = 0.0001) as compared to normal controls (139 IU/ml). There was no significant difference in the levels of CCL5 in patients with asthma (625 pg/ml) as compared to normal controls (663 pg/ml, P = 0. 57). No correlation was found between total IgE and CCL5 levels. IL-5 was not detected in patients with asthma or in controls. Moreover, the total counts of eosinophils in the blood did not increase in patients with asthma as compared to controls while eosinophils in sputum samples were increased in the former (mean =3.128%). Conclusion Plasma levels of CCL5 and IL-5 or eosinophil counts in the peripheral blood may not be useful diagnostic biomarkers to evaluate airway inflammation and monitor asthma severity. Conversely, the sputum eosinophil count may represent a useful diagnostic marker for assessing the magnitude of asthma exacerbation during sandstorms. |
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fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7249775</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2407959015</sourcerecordid><originalsourceid>FETCH-LOGICAL-c339t-3a6e85d8ab113ddfe3049f55b6781a8edf7516385b34b407ba9ff26c20ac3a893</originalsourceid><addsrcrecordid>eNpdkt9v1SAcxYlxccvck--GxJcZ7YRSCn0xmd3V3aSJS9RnQlt6y9bClR8z-9v2z0lzr3PbE5Dz4XC-4QDwBqMzxmj1qYtORX_GOKpegKMclzzjmBcvH-0PwYn31wghjFiOGHoFDklelIxxegTuV7dyijJoa6AdYJ3VsB7VbG-0UbDRG2l6SOFpXTf0_X_lI1yboNykYjot-rrJFv0u7OXl2sp6bex21BOsbTTBQ-nhlQ3KBC0n-EXbWbob5TxMHj9k7DW8SkHUQv7RYUzPOWt0B899GGcJL6LTZpNI0_tg3exfg4NBTl6d7Ndj8Ovr6md9mTXfv63r8ybrCKlCRmSpOO25bDEmfT8ogopqoLQtGceSq35gFJeE05YUbYFYK6thyMsuR7IjklfkGHze-W5jO6u-SwmdnMTW6TTAnbBSi6eK0aPY2FvB8qJKv5QMTvcGzv6Oygcxa9-paZJG2ehFXiCep__hKKHvnqHXNjqTxlsoVtEK4cXww47qnPXeqeEhDEZi6YXY9UIsvUj028f5H9h_LSB_AQNBtZc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2407959015</pqid></control><display><type>article</type><title>Evaluation of C-C Chemokine Ligand 5 (CCL5) Chemokine, Interleukin 5 (IL-5) Cytokine, and Eosinophil Counts as Potential Biomarkers in Saudi Patients with Chronic Asthma During Sandstorms</title><source>PubMed (Medline)</source><source>PubMed Central Open Access</source><creator>Alturaiki, Wael H</creator><creatorcontrib>Alturaiki, Wael H</creatorcontrib><description>Background and objectives Asthma is a common chronic inflammatory disorder of the lung that can be exacerbated by environmental triggers during sandstorms. This study aimed to evaluate the usefulness of C-C chemokine ligand 5 (CCL5) chemokine and interleukin 5 (IL-5) cytokine and determine the total eosinophil count in blood and sputum for use as biomarkers in Saudi patients with chronic asthma who visited emergency departments during sandstorms. Methods The study included 42 Saudi patients with chronic asthma and 20 healthy controls. Plasma levels of CCL5, IL-5, and total immunoglobulin E (IgE) were measured using a specific enzyme-linked immunosorbent assay (ELISA). Total eosinophils in peripheral blood were counted using a hematology analyzer (CELL-DYN Ruby System; Abbott Diagnostics, Chicago, Illinois); in sputum, eosinophils stained with Giemsa were examined under a microscope, counted, and expressed as a percentage of the total cells. Results Total IgE levels were significantly higher in patients with asthma (mean 433 IU/ml, P = 0.0001) as compared to normal controls (139 IU/ml). There was no significant difference in the levels of CCL5 in patients with asthma (625 pg/ml) as compared to normal controls (663 pg/ml, P = 0. 57). No correlation was found between total IgE and CCL5 levels. IL-5 was not detected in patients with asthma or in controls. Moreover, the total counts of eosinophils in the blood did not increase in patients with asthma as compared to controls while eosinophils in sputum samples were increased in the former (mean =3.128%). Conclusion Plasma levels of CCL5 and IL-5 or eosinophil counts in the peripheral blood may not be useful diagnostic biomarkers to evaluate airway inflammation and monitor asthma severity. Conversely, the sputum eosinophil count may represent a useful diagnostic marker for assessing the magnitude of asthma exacerbation during sandstorms.</description><identifier>ISSN: 2168-8184</identifier><identifier>EISSN: 2168-8184</identifier><identifier>DOI: 10.7759/cureus.7809</identifier><identifier>PMID: 32467785</identifier><language>eng</language><publisher>United States: Cureus Inc</publisher><subject>Allergy/Immunology ; Asthma ; Biomarkers ; Blood & organ donations ; Chemokines ; Chronic illnesses ; Cytokines ; Emergency medical care ; Environmental Health ; Enzymes ; Immunoglobulins ; Infections ; Inflammation ; Ligands ; Molecular weight ; Plasma ; Proteins ; Software</subject><ispartof>Curēus (Palo Alto, CA), 2020-04, Vol.12 (4), p.e7809-e7809</ispartof><rights>Copyright © 2020, Alturaiki et al.</rights><rights>Copyright © 2020, Alturaiki et al. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2020, Alturaiki et al. 2020 Alturaiki et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-3a6e85d8ab113ddfe3049f55b6781a8edf7516385b34b407ba9ff26c20ac3a893</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249775/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249775/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32467785$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alturaiki, Wael H</creatorcontrib><title>Evaluation of C-C Chemokine Ligand 5 (CCL5) Chemokine, Interleukin 5 (IL-5) Cytokine, and Eosinophil Counts as Potential Biomarkers in Saudi Patients with Chronic Asthma During Sandstorms</title><title>Curēus (Palo Alto, CA)</title><addtitle>Cureus</addtitle><description>Background and objectives Asthma is a common chronic inflammatory disorder of the lung that can be exacerbated by environmental triggers during sandstorms. This study aimed to evaluate the usefulness of C-C chemokine ligand 5 (CCL5) chemokine and interleukin 5 (IL-5) cytokine and determine the total eosinophil count in blood and sputum for use as biomarkers in Saudi patients with chronic asthma who visited emergency departments during sandstorms. Methods The study included 42 Saudi patients with chronic asthma and 20 healthy controls. Plasma levels of CCL5, IL-5, and total immunoglobulin E (IgE) were measured using a specific enzyme-linked immunosorbent assay (ELISA). Total eosinophils in peripheral blood were counted using a hematology analyzer (CELL-DYN Ruby System; Abbott Diagnostics, Chicago, Illinois); in sputum, eosinophils stained with Giemsa were examined under a microscope, counted, and expressed as a percentage of the total cells. Results Total IgE levels were significantly higher in patients with asthma (mean 433 IU/ml, P = 0.0001) as compared to normal controls (139 IU/ml). There was no significant difference in the levels of CCL5 in patients with asthma (625 pg/ml) as compared to normal controls (663 pg/ml, P = 0. 57). No correlation was found between total IgE and CCL5 levels. IL-5 was not detected in patients with asthma or in controls. Moreover, the total counts of eosinophils in the blood did not increase in patients with asthma as compared to controls while eosinophils in sputum samples were increased in the former (mean =3.128%). Conclusion Plasma levels of CCL5 and IL-5 or eosinophil counts in the peripheral blood may not be useful diagnostic biomarkers to evaluate airway inflammation and monitor asthma severity. Conversely, the sputum eosinophil count may represent a useful diagnostic marker for assessing the magnitude of asthma exacerbation during sandstorms.</description><subject>Allergy/Immunology</subject><subject>Asthma</subject><subject>Biomarkers</subject><subject>Blood & organ donations</subject><subject>Chemokines</subject><subject>Chronic illnesses</subject><subject>Cytokines</subject><subject>Emergency medical care</subject><subject>Environmental Health</subject><subject>Enzymes</subject><subject>Immunoglobulins</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Ligands</subject><subject>Molecular weight</subject><subject>Plasma</subject><subject>Proteins</subject><subject>Software</subject><issn>2168-8184</issn><issn>2168-8184</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkt9v1SAcxYlxccvck--GxJcZ7YRSCn0xmd3V3aSJS9RnQlt6y9bClR8z-9v2z0lzr3PbE5Dz4XC-4QDwBqMzxmj1qYtORX_GOKpegKMclzzjmBcvH-0PwYn31wghjFiOGHoFDklelIxxegTuV7dyijJoa6AdYJ3VsB7VbG-0UbDRG2l6SOFpXTf0_X_lI1yboNykYjot-rrJFv0u7OXl2sp6bex21BOsbTTBQ-nhlQ3KBC0n-EXbWbob5TxMHj9k7DW8SkHUQv7RYUzPOWt0B899GGcJL6LTZpNI0_tg3exfg4NBTl6d7Ndj8Ovr6md9mTXfv63r8ybrCKlCRmSpOO25bDEmfT8ogopqoLQtGceSq35gFJeE05YUbYFYK6thyMsuR7IjklfkGHze-W5jO6u-SwmdnMTW6TTAnbBSi6eK0aPY2FvB8qJKv5QMTvcGzv6Oygcxa9-paZJG2ehFXiCep__hKKHvnqHXNjqTxlsoVtEK4cXww47qnPXeqeEhDEZi6YXY9UIsvUj028f5H9h_LSB_AQNBtZc</recordid><startdate>20200424</startdate><enddate>20200424</enddate><creator>Alturaiki, Wael H</creator><general>Cureus Inc</general><general>Cureus</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200424</creationdate><title>Evaluation of C-C Chemokine Ligand 5 (CCL5) Chemokine, Interleukin 5 (IL-5) Cytokine, and Eosinophil Counts as Potential Biomarkers in Saudi Patients with Chronic Asthma During Sandstorms</title><author>Alturaiki, Wael H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-3a6e85d8ab113ddfe3049f55b6781a8edf7516385b34b407ba9ff26c20ac3a893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Allergy/Immunology</topic><topic>Asthma</topic><topic>Biomarkers</topic><topic>Blood & organ donations</topic><topic>Chemokines</topic><topic>Chronic illnesses</topic><topic>Cytokines</topic><topic>Emergency medical care</topic><topic>Environmental Health</topic><topic>Enzymes</topic><topic>Immunoglobulins</topic><topic>Infections</topic><topic>Inflammation</topic><topic>Ligands</topic><topic>Molecular weight</topic><topic>Plasma</topic><topic>Proteins</topic><topic>Software</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alturaiki, Wael H</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Curēus (Palo Alto, CA)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alturaiki, Wael H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of C-C Chemokine Ligand 5 (CCL5) Chemokine, Interleukin 5 (IL-5) Cytokine, and Eosinophil Counts as Potential Biomarkers in Saudi Patients with Chronic Asthma During Sandstorms</atitle><jtitle>Curēus (Palo Alto, CA)</jtitle><addtitle>Cureus</addtitle><date>2020-04-24</date><risdate>2020</risdate><volume>12</volume><issue>4</issue><spage>e7809</spage><epage>e7809</epage><pages>e7809-e7809</pages><issn>2168-8184</issn><eissn>2168-8184</eissn><abstract>Background and objectives Asthma is a common chronic inflammatory disorder of the lung that can be exacerbated by environmental triggers during sandstorms. This study aimed to evaluate the usefulness of C-C chemokine ligand 5 (CCL5) chemokine and interleukin 5 (IL-5) cytokine and determine the total eosinophil count in blood and sputum for use as biomarkers in Saudi patients with chronic asthma who visited emergency departments during sandstorms. Methods The study included 42 Saudi patients with chronic asthma and 20 healthy controls. Plasma levels of CCL5, IL-5, and total immunoglobulin E (IgE) were measured using a specific enzyme-linked immunosorbent assay (ELISA). Total eosinophils in peripheral blood were counted using a hematology analyzer (CELL-DYN Ruby System; Abbott Diagnostics, Chicago, Illinois); in sputum, eosinophils stained with Giemsa were examined under a microscope, counted, and expressed as a percentage of the total cells. Results Total IgE levels were significantly higher in patients with asthma (mean 433 IU/ml, P = 0.0001) as compared to normal controls (139 IU/ml). There was no significant difference in the levels of CCL5 in patients with asthma (625 pg/ml) as compared to normal controls (663 pg/ml, P = 0. 57). No correlation was found between total IgE and CCL5 levels. IL-5 was not detected in patients with asthma or in controls. Moreover, the total counts of eosinophils in the blood did not increase in patients with asthma as compared to controls while eosinophils in sputum samples were increased in the former (mean =3.128%). Conclusion Plasma levels of CCL5 and IL-5 or eosinophil counts in the peripheral blood may not be useful diagnostic biomarkers to evaluate airway inflammation and monitor asthma severity. Conversely, the sputum eosinophil count may represent a useful diagnostic marker for assessing the magnitude of asthma exacerbation during sandstorms.</abstract><cop>United States</cop><pub>Cureus Inc</pub><pmid>32467785</pmid><doi>10.7759/cureus.7809</doi><oa>free_for_read</oa></addata></record> |
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subjects | Allergy/Immunology Asthma Biomarkers Blood & organ donations Chemokines Chronic illnesses Cytokines Emergency medical care Environmental Health Enzymes Immunoglobulins Infections Inflammation Ligands Molecular weight Plasma Proteins Software |
title | Evaluation of C-C Chemokine Ligand 5 (CCL5) Chemokine, Interleukin 5 (IL-5) Cytokine, and Eosinophil Counts as Potential Biomarkers in Saudi Patients with Chronic Asthma During Sandstorms |
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