Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD
Sphingomyelins (SMs) are a class of relevant bioactive molecules that act as key modulators of different cellular processes, such as growth arrest, exosome formation, and the inflammatory response influenced by many environmental conditions, leading to pyroptosis, a form of programmed cell death due...
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| Veröffentlicht in: | International journal of molecular sciences 2020-05, Vol.21 (9), p.3281 |
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| creator | Cataldi, Samuela Borrelli, Antonella Ceccarini, Maria Rachele Nakashidze, Irina Codini, Michela Belov, Oleg Ivanov, Alexander Krasavin, Eugene Ferri, Ivana Conte, Carmela Patria, Federica Filomena Beccari, Tommaso Mancini, Aldo Curcio, Francesco Ambesi-Impiombato, Francesco Saverio Albi, Elisabetta |
| description | Sphingomyelins (SMs) are a class of relevant bioactive molecules that act as key modulators of different cellular processes, such as growth arrest, exosome formation, and the inflammatory response influenced by many environmental conditions, leading to pyroptosis, a form of programmed cell death due to Caspase-1 involvement. To study liver pyroptosis and hepatic SM metabolism via both lysosomal acid SMase (aSMase) and endoplasmic reticulum/nucleus neutral SMase (nSMase) during the exposure of mice to radiation and to ascertain if this process can be modulated by protective molecules, we used an experimental design (previously used by us) to evaluate the effects of both ionizing radiation and a specific protective molecule (rMnSOD) in the brain in collaboration with the Joint Institute for Nuclear Research, Dubna (Russia). As shown by the Caspase-1 immunostaining of the liver sections, the radiation resulted in the loss of the normal cell structure alongside a progressive and dose-dependent increase of the labelling, treatment, and pretreatment with rMnSOD, which had a significant protective effect on the livers. SM metabolic analyses, performed on aSMase and nSMase gene expression, as well as protein content and activity, proved that rMnSOD was able to significantly reduce radiation-induced damage by playing both a protective role via aSMase and a preventive role via nSMase. |
| doi_str_mv | 10.3390/ijms21093281 |
| format | Article |
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To study liver pyroptosis and hepatic SM metabolism via both lysosomal acid SMase (aSMase) and endoplasmic reticulum/nucleus neutral SMase (nSMase) during the exposure of mice to radiation and to ascertain if this process can be modulated by protective molecules, we used an experimental design (previously used by us) to evaluate the effects of both ionizing radiation and a specific protective molecule (rMnSOD) in the brain in collaboration with the Joint Institute for Nuclear Research, Dubna (Russia). As shown by the Caspase-1 immunostaining of the liver sections, the radiation resulted in the loss of the normal cell structure alongside a progressive and dose-dependent increase of the labelling, treatment, and pretreatment with rMnSOD, which had a significant protective effect on the livers. SM metabolic analyses, performed on aSMase and nSMase gene expression, as well as protein content and activity, proved that rMnSOD was able to significantly reduce radiation-induced damage by playing both a protective role via aSMase and a preventive role via nSMase.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms21093281</identifier><identifier>PMID: 32384654</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animals ; Apoptosis ; Brain research ; Caspase 1 - metabolism ; Caspase-1 ; Cell cycle ; Cell death ; Cell growth ; Cytology ; Design of experiments ; Endoplasmic reticulum ; Environmental conditions ; Enzymes ; Female ; Gene expression ; Immunomodulation ; Inflammation ; Inflammatory response ; Ionizing radiation ; Labeling ; Liver ; Liver - drug effects ; Liver - metabolism ; Liver - radiation effects ; Metabolism ; Mice ; Nuclear research ; Pretreatment ; Proteins ; Pyroptosis ; Radiation damage ; Radiation effects ; Radiation Injuries - drug therapy ; Radiation Injuries - metabolism ; Radiation-Protective Agents - pharmacology ; Radiation-Protective Agents - therapeutic use ; Sphingomyelin phosphodiesterase ; Sphingomyelin Phosphodiesterase - metabolism ; Sphingomyelins - metabolism ; Thyroid gland</subject><ispartof>International journal of molecular sciences, 2020-05, Vol.21 (9), p.3281</ispartof><rights>2020. 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SM metabolic analyses, performed on aSMase and nSMase gene expression, as well as protein content and activity, proved that rMnSOD was able to significantly reduce radiation-induced damage by playing both a protective role via aSMase and a preventive role via nSMase.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Brain research</subject><subject>Caspase 1 - metabolism</subject><subject>Caspase-1</subject><subject>Cell cycle</subject><subject>Cell death</subject><subject>Cell growth</subject><subject>Cytology</subject><subject>Design of experiments</subject><subject>Endoplasmic reticulum</subject><subject>Environmental conditions</subject><subject>Enzymes</subject><subject>Female</subject><subject>Gene expression</subject><subject>Immunomodulation</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Ionizing radiation</subject><subject>Labeling</subject><subject>Liver</subject><subject>Liver - drug effects</subject><subject>Liver - 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| fulltext | fulltext |
| identifier | ISSN: 1422-0067 |
| ispartof | International journal of molecular sciences, 2020-05, Vol.21 (9), p.3281 |
| issn | 1422-0067 1661-6596 1422-0067 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7247354 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Animals Apoptosis Brain research Caspase 1 - metabolism Caspase-1 Cell cycle Cell death Cell growth Cytology Design of experiments Endoplasmic reticulum Environmental conditions Enzymes Female Gene expression Immunomodulation Inflammation Inflammatory response Ionizing radiation Labeling Liver Liver - drug effects Liver - metabolism Liver - radiation effects Metabolism Mice Nuclear research Pretreatment Proteins Pyroptosis Radiation damage Radiation effects Radiation Injuries - drug therapy Radiation Injuries - metabolism Radiation-Protective Agents - pharmacology Radiation-Protective Agents - therapeutic use Sphingomyelin phosphodiesterase Sphingomyelin Phosphodiesterase - metabolism Sphingomyelins - metabolism Thyroid gland |
| title | Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T00%3A12%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Acid%20and%20Neutral%20Sphingomyelinase%20Behavior%20in%20Radiation-Induced%20Liver%20Pyroptosis%20and%20in%20the%20Protective/Preventive%20Role%20of%20rMnSOD&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Cataldi,%20Samuela&rft.date=2020-05-06&rft.volume=21&rft.issue=9&rft.spage=3281&rft.pages=3281-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms21093281&rft_dat=%3Cproquest_pubme%3E2400716757%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2400716757&rft_id=info:pmid/32384654&rfr_iscdi=true |