Comet Test in Saliva Leukocytes of Pre-School Children Exposed to Air Pollution in North Italy: The Respira Study
Air pollution is a well-known problem for human health, especially for children living in highly polluted urban areas. This study aimed to assess the relationship between airborne pollutants concentration and biomarkers of DNA damage in the buccal mucosa cells of pre-school children. DNA damage was...
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| Veröffentlicht in: | International journal of environmental research and public health 2020-05, Vol.17 (9), p.3276 |
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| creator | Zani, Claudia Ceretti, Elisabetta Zerbini, Ilaria Viola, Gaia Claudia Viviana Donato, Francesco Gelatti, Umberto Feretti, Donatella |
| description | Air pollution is a well-known problem for human health, especially for children living in highly polluted urban areas. This study aimed to assess the relationship between airborne pollutants concentration and biomarkers of DNA damage in the buccal mucosa cells of pre-school children. DNA damage was investigated with comet test in saliva leukocytes taken from sputum of 3- to 6-year-old children living in Brescia, Northern Italy, collected during two consecutive winter seasons (2012-2013). The daily levels of PM10, PM2.5, NO
, CO, SO
, benzene and O
in urban air were collected for the whole period. A questionnaire filled in by the children's parents was used to evaluate indoor and outdoor exposure. DNA damage in saliva leukocytes was evaluated in 152 children and the means of tail intensity and visual score as DNA damage were 6.2 ± 4.3 and 182.1 ± 30.9, respectively. No demographic and indoor or outdoor exposure variable was associated with the two measures of DNA damage. No significant association between air pollution and DNA damage in children's buccal leukocytes was found. In this study, the comet assay does not appear to be a valuable biomarker to detect DNA damage in children exposed to high levels of air pollutants, such as PM10, PM2.5 and NO
. |
| doi_str_mv | 10.3390/ijerph17093276 |
| format | Article |
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, CO, SO
, benzene and O
in urban air were collected for the whole period. A questionnaire filled in by the children's parents was used to evaluate indoor and outdoor exposure. DNA damage in saliva leukocytes was evaluated in 152 children and the means of tail intensity and visual score as DNA damage were 6.2 ± 4.3 and 182.1 ± 30.9, respectively. No demographic and indoor or outdoor exposure variable was associated with the two measures of DNA damage. No significant association between air pollution and DNA damage in children's buccal leukocytes was found. In this study, the comet assay does not appear to be a valuable biomarker to detect DNA damage in children exposed to high levels of air pollutants, such as PM10, PM2.5 and NO
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, CO, SO
, benzene and O
in urban air were collected for the whole period. A questionnaire filled in by the children's parents was used to evaluate indoor and outdoor exposure. DNA damage in saliva leukocytes was evaluated in 152 children and the means of tail intensity and visual score as DNA damage were 6.2 ± 4.3 and 182.1 ± 30.9, respectively. No demographic and indoor or outdoor exposure variable was associated with the two measures of DNA damage. No significant association between air pollution and DNA damage in children's buccal leukocytes was found. In this study, the comet assay does not appear to be a valuable biomarker to detect DNA damage in children exposed to high levels of air pollutants, such as PM10, PM2.5 and NO
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This study aimed to assess the relationship between airborne pollutants concentration and biomarkers of DNA damage in the buccal mucosa cells of pre-school children. DNA damage was investigated with comet test in saliva leukocytes taken from sputum of 3- to 6-year-old children living in Brescia, Northern Italy, collected during two consecutive winter seasons (2012-2013). The daily levels of PM10, PM2.5, NO
, CO, SO
, benzene and O
in urban air were collected for the whole period. A questionnaire filled in by the children's parents was used to evaluate indoor and outdoor exposure. DNA damage in saliva leukocytes was evaluated in 152 children and the means of tail intensity and visual score as DNA damage were 6.2 ± 4.3 and 182.1 ± 30.9, respectively. No demographic and indoor or outdoor exposure variable was associated with the two measures of DNA damage. No significant association between air pollution and DNA damage in children's buccal leukocytes was found. In this study, the comet assay does not appear to be a valuable biomarker to detect DNA damage in children exposed to high levels of air pollutants, such as PM10, PM2.5 and NO
.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>32397090</pmid><doi>10.3390/ijerph17093276</doi><orcidid>https://orcid.org/0000-0001-7888-5716</orcidid><orcidid>https://orcid.org/0000-0002-9337-2166</orcidid><orcidid>https://orcid.org/0000-0002-8455-6174</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Air Pollutants - analysis Air Pollutants - toxicity Air pollution Air pollution measurements Benzene Biomarkers Buccal mucosa Child Child, Preschool Children Comet Assay Damage detection Deoxyribonucleic acid DNA DNA Damage Environmental Exposure - adverse effects Environmental Exposure - analysis Environmental health Evaluation Exposure Female Humans Hydrocarbons Italy Leukocytes Male Nitrogen dioxide Parents & parenting Particulate matter Particulate Matter - analysis Particulate Matter - toxicity Pollutants Preschool children Public health Questionnaires Regression analysis Saliva Saliva - chemistry Software Sputum Sulfur dioxide Toxicity Urban areas |
| title | Comet Test in Saliva Leukocytes of Pre-School Children Exposed to Air Pollution in North Italy: The Respira Study |
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