High efficiency CHO cell display-based antibody maturation

Previously, we developed a CHO cell display-based antibody maturation procedure in which an antibody (or other protein) gene of interest was induced to mutate by activation-induced cytidine deaminase (AID) and then form a library by simply proliferating the CHO cells in culture. In this study, we fu...

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Veröffentlicht in:	Scientific reports 2020-05, Vol.10 (1), p.8102-8102, Article 8102
Hauptverfasser:	Luo, Ruiqi, Zhao, Yun, Fan, Yingjun, An, Lili, Jiang, Tao, Ma, Shaohua, Hang, Haiying
Format:	Artikel
Sprache:	eng
Schlagworte:	13/1 13/109 13/31 14 14/35 38 38/22 38/23 38/77 631/154/1435/2163 631/1647/664/2228 82/103 82/80 82/83 Activation-induced cytidine deaminase Animals Antibodies Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - genetics Antibodies, Monoclonal - immunology Cell culture CHO Cells Cricetinae Cricetulus Cytidine deaminase Cytidine Deaminase - genetics Cytidine Deaminase - immunology Efficiency Gene Library Humanities and Social Sciences Humans Maturation multidisciplinary Mutation Mutation Rate Mutation rates Nucleic acids Science Science (multidisciplinary) Single-Chain Antibodies - biosynthesis Single-Chain Antibodies - genetics Single-Chain Antibodies - immunology Transfection Tumor Necrosis Factor-alpha - antagonists & inhibitors Tumor Necrosis Factor-alpha - immunology
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