High efficiency CHO cell display-based antibody maturation
Previously, we developed a CHO cell display-based antibody maturation procedure in which an antibody (or other protein) gene of interest was induced to mutate by activation-induced cytidine deaminase (AID) and then form a library by simply proliferating the CHO cells in culture. In this study, we fu...
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| Veröffentlicht in: | Scientific reports 2020-05, Vol.10 (1), p.8102-8102, Article 8102 |
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| creator | Luo, Ruiqi Zhao, Yun Fan, Yingjun An, Lili Jiang, Tao Ma, Shaohua Hang, Haiying |
| description | Previously, we developed a CHO cell display-based antibody maturation procedure in which an antibody (or other protein) gene of interest was induced to mutate by activation-induced cytidine deaminase (AID) and then form a library by simply proliferating the CHO cells in culture. In this study, we further improved the efficiency of this maturation system by reengineering AID, and optimizing the nucleic acid sequence of the target antibody gene and
AID
gene as well as the protocol for
AID
gene transfection. These changes have increased both the mutation rate and the number of mutation type of antibody genes by more than 10 fold, and greatly improved the maturation efficiency of antibody/other proteins. |
| doi_str_mv | 10.1038/s41598-020-65044-7 |
| format | Article |
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AID
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AID
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AID
gene as well as the protocol for
AID
gene transfection. 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biosynthesis</topic><topic>Antibodies, Monoclonal - genetics</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Cell culture</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Cytidine deaminase</topic><topic>Cytidine Deaminase - genetics</topic><topic>Cytidine Deaminase - immunology</topic><topic>Efficiency</topic><topic>Gene Library</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Maturation</topic><topic>multidisciplinary</topic><topic>Mutation</topic><topic>Mutation Rate</topic><topic>Mutation rates</topic><topic>Nucleic acids</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Single-Chain Antibodies - biosynthesis</topic><topic>Single-Chain Antibodies - genetics</topic><topic>Single-Chain Antibodies - immunology</topic><topic>Transfection</topic><topic>Tumor Necrosis Factor-alpha - antagonists & inhibitors</topic><topic>Tumor Necrosis Factor-alpha - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Ruiqi</creatorcontrib><creatorcontrib>Zhao, Yun</creatorcontrib><creatorcontrib>Fan, Yingjun</creatorcontrib><creatorcontrib>An, Lili</creatorcontrib><creatorcontrib>Jiang, Tao</creatorcontrib><creatorcontrib>Ma, Shaohua</creatorcontrib><creatorcontrib>Hang, Haiying</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Ruiqi</au><au>Zhao, Yun</au><au>Fan, Yingjun</au><au>An, Lili</au><au>Jiang, Tao</au><au>Ma, Shaohua</au><au>Hang, Haiying</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High efficiency CHO cell display-based antibody maturation</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-05-15</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>8102</spage><epage>8102</epage><pages>8102-8102</pages><artnum>8102</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Previously, we developed a CHO cell display-based antibody maturation procedure in which an antibody (or other protein) gene of interest was induced to mutate by activation-induced cytidine deaminase (AID) and then form a library by simply proliferating the CHO cells in culture. In this study, we further improved the efficiency of this maturation system by reengineering AID, and optimizing the nucleic acid sequence of the target antibody gene and
AID
gene as well as the protocol for
AID
gene transfection. These changes have increased both the mutation rate and the number of mutation type of antibody genes by more than 10 fold, and greatly improved the maturation efficiency of antibody/other proteins.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32415149</pmid><doi>10.1038/s41598-020-65044-7</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-7399-6975</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 13/1 13/109 13/31 14 14/35 38 38/22 38/23 38/77 631/154/1435/2163 631/1647/664/2228 82/103 82/80 82/83 Activation-induced cytidine deaminase Animals Antibodies Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - genetics Antibodies, Monoclonal - immunology Cell culture CHO Cells Cricetinae Cricetulus Cytidine deaminase Cytidine Deaminase - genetics Cytidine Deaminase - immunology Efficiency Gene Library Humanities and Social Sciences Humans Maturation multidisciplinary Mutation Mutation Rate Mutation rates Nucleic acids Science Science (multidisciplinary) Single-Chain Antibodies - biosynthesis Single-Chain Antibodies - genetics Single-Chain Antibodies - immunology Transfection Tumor Necrosis Factor-alpha - antagonists & inhibitors Tumor Necrosis Factor-alpha - immunology |
| title | High efficiency CHO cell display-based antibody maturation |
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