Prostaglandin F2α agonist-induced suppression of 3T3-L1 cell adipogenesis affects spatial formation of extra-cellular matrix
To establish a deepening of the upper eyelid sulcus (DUES) model that can be induced by prostaglandin (PG) analogues, a three-dimension (3D) tissue culture was employed. Upon adipogenesis of the 3T3-L1 organoid, the effects of either Bimatoprost acid (BIM-A), or PGF2α were examined. During the adipo...
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| Veröffentlicht in: | Scientific reports 2020-05, Vol.10 (1), p.7958-7958, Article 7958 |
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| description | To establish a deepening of the upper eyelid sulcus (DUES) model that can be induced by prostaglandin (PG) analogues, a three-dimension (3D) tissue culture was employed. Upon adipogenesis of the 3T3-L1 organoid, the effects of either Bimatoprost acid (BIM-A), or PGF2α were examined. During the adipogenesis, organoid size, lipid staining by BODIPY and expression of the extracellular matrix (ECM) by immunocytochemistry and/or quantitative PCR were employed. The size of the organoid increased remarkably during the adipogenesis, while such increases were significantly inhibited by the presence of PGF2α or BIM-A. BODIPY positive lipid-laden cells significantly increased during the adipogenesis, while in contrast they were greatly suppressed by the presence of PGF2α. Characteristic and spatial changes in ECM expressions observed upon adipogenesis were greatly modified by the presence of PGs. Our present study using a 3D tissue culture may be a suitable strategy toward understanding disease etiology of DUES. |
| doi_str_mv | 10.1038/s41598-020-64674-1 |
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Upon adipogenesis of the 3T3-L1 organoid, the effects of either Bimatoprost acid (BIM-A), or PGF2α were examined. During the adipogenesis, organoid size, lipid staining by BODIPY and expression of the extracellular matrix (ECM) by immunocytochemistry and/or quantitative PCR were employed. The size of the organoid increased remarkably during the adipogenesis, while such increases were significantly inhibited by the presence of PGF2α or BIM-A. BODIPY positive lipid-laden cells significantly increased during the adipogenesis, while in contrast they were greatly suppressed by the presence of PGF2α. Characteristic and spatial changes in ECM expressions observed upon adipogenesis were greatly modified by the presence of PGs. Our present study using a 3D tissue culture may be a suitable strategy toward understanding disease etiology of DUES.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-64674-1</identifier><identifier>PMID: 32409724</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/1 ; 13/106 ; 3T3-L1 Cells ; 631/154/309/436/108 ; 692/699/3161/3169/3170 ; Adipocytes ; Adipogenesis ; Adipogenesis - drug effects ; Animals ; Cell culture ; Dinoprost - agonists ; Etiology ; Extracellular matrix ; Extracellular Matrix - drug effects ; Extracellular Matrix - metabolism ; Eyelid ; Fatty acids ; Gene expression ; Growth factors ; Humanities and Social Sciences ; Immunocytochemistry ; Lipids ; Mice ; multidisciplinary ; Organoids ; Science ; Science (multidisciplinary) ; Time Factors ; Tissue culture</subject><ispartof>Scientific reports, 2020-05, Vol.10 (1), p.7958-7958, Article 7958</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. 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Hikage, Fumihito ; Itoh, Kaku ; Ida, Haruka ; Ohguro, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-43c5bcb93d166ef8eee003a4fda223fc72268ba642ebf489ec682bf75ea7b0b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>13/1</topic><topic>13/106</topic><topic>3T3-L1 Cells</topic><topic>631/154/309/436/108</topic><topic>692/699/3161/3169/3170</topic><topic>Adipocytes</topic><topic>Adipogenesis</topic><topic>Adipogenesis - drug effects</topic><topic>Animals</topic><topic>Cell culture</topic><topic>Dinoprost - agonists</topic><topic>Etiology</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix - drug effects</topic><topic>Extracellular Matrix - metabolism</topic><topic>Eyelid</topic><topic>Fatty acids</topic><topic>Gene expression</topic><topic>Growth factors</topic><topic>Humanities and Social Sciences</topic><topic>Immunocytochemistry</topic><topic>Lipids</topic><topic>Mice</topic><topic>multidisciplinary</topic><topic>Organoids</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Time Factors</topic><topic>Tissue culture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ida, Yosuke</creatorcontrib><creatorcontrib>Hikage, Fumihito</creatorcontrib><creatorcontrib>Itoh, Kaku</creatorcontrib><creatorcontrib>Ida, Haruka</creatorcontrib><creatorcontrib>Ohguro, Hiroshi</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ida, Yosuke</au><au>Hikage, Fumihito</au><au>Itoh, Kaku</au><au>Ida, Haruka</au><au>Ohguro, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prostaglandin F2α agonist-induced suppression of 3T3-L1 cell adipogenesis affects spatial formation of extra-cellular matrix</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-05-14</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>7958</spage><epage>7958</epage><pages>7958-7958</pages><artnum>7958</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>To establish a deepening of the upper eyelid sulcus (DUES) model that can be induced by prostaglandin (PG) analogues, a three-dimension (3D) tissue culture was employed. Upon adipogenesis of the 3T3-L1 organoid, the effects of either Bimatoprost acid (BIM-A), or PGF2α were examined. During the adipogenesis, organoid size, lipid staining by BODIPY and expression of the extracellular matrix (ECM) by immunocytochemistry and/or quantitative PCR were employed. The size of the organoid increased remarkably during the adipogenesis, while such increases were significantly inhibited by the presence of PGF2α or BIM-A. BODIPY positive lipid-laden cells significantly increased during the adipogenesis, while in contrast they were greatly suppressed by the presence of PGF2α. Characteristic and spatial changes in ECM expressions observed upon adipogenesis were greatly modified by the presence of PGs. Our present study using a 3D tissue culture may be a suitable strategy toward understanding disease etiology of DUES.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32409724</pmid><doi>10.1038/s41598-020-64674-1</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/1 13/106 3T3-L1 Cells 631/154/309/436/108 692/699/3161/3169/3170 Adipocytes Adipogenesis Adipogenesis - drug effects Animals Cell culture Dinoprost - agonists Etiology Extracellular matrix Extracellular Matrix - drug effects Extracellular Matrix - metabolism Eyelid Fatty acids Gene expression Growth factors Humanities and Social Sciences Immunocytochemistry Lipids Mice multidisciplinary Organoids Science Science (multidisciplinary) Time Factors Tissue culture |
| title | Prostaglandin F2α agonist-induced suppression of 3T3-L1 cell adipogenesis affects spatial formation of extra-cellular matrix |
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