Canine parvovirus 2b in fecal samples of asymptomatic free-living South American coatis (Nasua nasua, Linnaeus, 1766)
Canine parvovirus type 2 (CPV-2) is classified into three subtypes (CPV-2a, CPV-2b, and CPV-2c) and is the main cause of enteritis and myocarditis in young domestic and wild animals. This study aimed to evaluate the presence of CPV-2 in the feces of asymptomatic free-living coatis from Garden Forest...
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| Veröffentlicht in: | Brazilian journal of microbiology 2020-09, Vol.51 (3), p.1399-1403 |
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| creator | Spera, Caroline Giuseppa Lorenzetti, Elis Lavorente, Fernanda Louise Pereira de Calasans Marques, Gustavo Bisca, Jacqueline Muniz Teixeira, Carlos Roberto Alfieri, Amauri Alcindo Alfieri, Alice Fernandes |
| description | Canine parvovirus type 2 (CPV-2) is classified into three subtypes (CPV-2a, CPV-2b, and CPV-2c) and is the main cause of enteritis and myocarditis in young domestic and wild animals. This study aimed to evaluate the presence of CPV-2 in the feces of asymptomatic free-living coatis from Garden Forest Reserve, Palmital city, SP, Brazil. Fecal samples from 21 coatis (both sexes, different ages, and different aspects of feces) were collected in August 2014 and March 2015. The nucleic acid extracted was submitted to a polymerase chain reaction (PCR) assay to amplify a fragment of the VP2 gene of CPV-2. Eight (38%) fecal samples were positive in the PCR assay and were confirmed by sequencing. The 7 nucleotide (nt) sequences analyzed showed 100% nt identity with the prototype strain of CPV-2b (CPV-39 strain). The analysis of the deduced amino acid (aa) sequence revealed the presence of the GAT codon (aa D-Asp) at position 426 of the VP2 viral protein (subtype 2b). This study describes for the first time the identification of CPV-2b in asymptomatic free-living coatis (
Nasua nasua
) and suggests that coatis are susceptible to
Carnivore protoparvovirus 1
infection and are important as a reservoir and an asymptomatic carrier to other wild and domestic animal species. |
| doi_str_mv | 10.1007/s42770-020-00293-2 |
| format | Article |
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Nasua nasua
) and suggests that coatis are susceptible to
Carnivore protoparvovirus 1
infection and are important as a reservoir and an asymptomatic carrier to other wild and domestic animal species.</description><identifier>ISSN: 1517-8382</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1007/s42770-020-00293-2</identifier><identifier>PMID: 32406051</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Amino Acid Sequence ; Amino acids ; Animal species ; Animals ; Asymptomatic ; Base Sequence ; Biomedical and Life Sciences ; Brazil ; Coating ; Dogs ; Domestic animals ; Enteritis ; Feces ; Feces - virology ; Female ; Food Microbiology ; Heart diseases ; Life Sciences ; Male ; Medical Microbiology ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Mycology ; Myocarditis ; Nasua nasua ; Nucleic acids ; Nucleotides ; Parvoviridae Infections - veterinary ; Parvoviridae Infections - virology ; Parvovirus, Canine - classification ; Parvovirus, Canine - genetics ; Parvovirus, Canine - isolation & purification ; Parvoviruses ; Phylogeny ; Polymerase chain reaction ; Procyonidae - virology ; Veterinary Microbiology - Research Paper ; Wild animals</subject><ispartof>Brazilian journal of microbiology, 2020-09, Vol.51 (3), p.1399-1403</ispartof><rights>Sociedade Brasileira de Microbiologia 2020</rights><rights>Sociedade Brasileira de Microbiologia 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-6a03a3178a02633063073935cf8c1ddab5d80d53f3d46e1f7fd3eca07230e0653</citedby><cites>FETCH-LOGICAL-c474t-6a03a3178a02633063073935cf8c1ddab5d80d53f3d46e1f7fd3eca07230e0653</cites><orcidid>0000-0002-8607-956X ; 0000-0002-5032-712X ; 0000-0002-7024-4487</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223230/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223230/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,41469,42538,51300,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32406051$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Spera, Caroline Giuseppa</creatorcontrib><creatorcontrib>Lorenzetti, Elis</creatorcontrib><creatorcontrib>Lavorente, Fernanda Louise Pereira</creatorcontrib><creatorcontrib>de Calasans Marques, Gustavo</creatorcontrib><creatorcontrib>Bisca, Jacqueline Muniz</creatorcontrib><creatorcontrib>Teixeira, Carlos Roberto</creatorcontrib><creatorcontrib>Alfieri, Amauri Alcindo</creatorcontrib><creatorcontrib>Alfieri, Alice Fernandes</creatorcontrib><title>Canine parvovirus 2b in fecal samples of asymptomatic free-living South American coatis (Nasua nasua, Linnaeus, 1766)</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><addtitle>Braz J Microbiol</addtitle><description>Canine parvovirus type 2 (CPV-2) is classified into three subtypes (CPV-2a, CPV-2b, and CPV-2c) and is the main cause of enteritis and myocarditis in young domestic and wild animals. This study aimed to evaluate the presence of CPV-2 in the feces of asymptomatic free-living coatis from Garden Forest Reserve, Palmital city, SP, Brazil. Fecal samples from 21 coatis (both sexes, different ages, and different aspects of feces) were collected in August 2014 and March 2015. The nucleic acid extracted was submitted to a polymerase chain reaction (PCR) assay to amplify a fragment of the VP2 gene of CPV-2. Eight (38%) fecal samples were positive in the PCR assay and were confirmed by sequencing. The 7 nucleotide (nt) sequences analyzed showed 100% nt identity with the prototype strain of CPV-2b (CPV-39 strain). The analysis of the deduced amino acid (aa) sequence revealed the presence of the GAT codon (aa D-Asp) at position 426 of the VP2 viral protein (subtype 2b). This study describes for the first time the identification of CPV-2b in asymptomatic free-living coatis (
Nasua nasua
) and suggests that coatis are susceptible to
Carnivore protoparvovirus 1
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This study aimed to evaluate the presence of CPV-2 in the feces of asymptomatic free-living coatis from Garden Forest Reserve, Palmital city, SP, Brazil. Fecal samples from 21 coatis (both sexes, different ages, and different aspects of feces) were collected in August 2014 and March 2015. The nucleic acid extracted was submitted to a polymerase chain reaction (PCR) assay to amplify a fragment of the VP2 gene of CPV-2. Eight (38%) fecal samples were positive in the PCR assay and were confirmed by sequencing. The 7 nucleotide (nt) sequences analyzed showed 100% nt identity with the prototype strain of CPV-2b (CPV-39 strain). The analysis of the deduced amino acid (aa) sequence revealed the presence of the GAT codon (aa D-Asp) at position 426 of the VP2 viral protein (subtype 2b). This study describes for the first time the identification of CPV-2b in asymptomatic free-living coatis (
Nasua nasua
) and suggests that coatis are susceptible to
Carnivore protoparvovirus 1
infection and are important as a reservoir and an asymptomatic carrier to other wild and domestic animal species.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>32406051</pmid><doi>10.1007/s42770-020-00293-2</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-8607-956X</orcidid><orcidid>https://orcid.org/0000-0002-5032-712X</orcidid><orcidid>https://orcid.org/0000-0002-7024-4487</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Brazilian journal of microbiology, 2020-09, Vol.51 (3), p.1399-1403 |
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| subjects | Amino Acid Sequence Amino acids Animal species Animals Asymptomatic Base Sequence Biomedical and Life Sciences Brazil Coating Dogs Domestic animals Enteritis Feces Feces - virology Female Food Microbiology Heart diseases Life Sciences Male Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbiology Mycology Myocarditis Nasua nasua Nucleic acids Nucleotides Parvoviridae Infections - veterinary Parvoviridae Infections - virology Parvovirus, Canine - classification Parvovirus, Canine - genetics Parvovirus, Canine - isolation & purification Parvoviruses Phylogeny Polymerase chain reaction Procyonidae - virology Veterinary Microbiology - Research Paper Wild animals |
| title | Canine parvovirus 2b in fecal samples of asymptomatic free-living South American coatis (Nasua nasua, Linnaeus, 1766) |
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