Comparative gene expression analysis of Fas and related genes in preeclamptic and healthy women: A cross-sectional study
Preeclampsia is a hypertensive disorder of pregnancy affecting about 2-10% pregnancies worldwide. mRNA expression of tumor necrosis factor alpha and have been reported to be altered in placental bed in preeclamptic pregnancies. We hypothesized that the expression of these genes is also altered in pe...
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| Veröffentlicht in: | International Journal of Reproductive BioMedicine 2020-04, Vol.18 (4), p.235-242 |
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| creator | Ali, Zaima Khaliq, Saba Zaki, Saima Ahmad, Hafiz Usman Lone, Khalid Pervaiz |
| description | Preeclampsia is a hypertensive disorder of pregnancy affecting about 2-10% pregnancies worldwide. mRNA expression of tumor necrosis factor alpha
and
have been reported to be altered in placental bed in preeclamptic pregnancies. We hypothesized that the expression of these genes is also altered in peripheral blood mononuclear cells (PBMCs) in preeclampsia.
To compare the expression of Fas receptor and related genes in PBMCs of preeclamptic and normotensive pregnant women.
A cross-sectional comparative study comprising of 18 cases and 18 controls was designed. 5 ml of venous blood was drawn and collected considering aseptic measures. Buffy coat was separated by centrifugation and stored at -20°C. Favor Prep total RNA Isolation Kit (Favorgen, Taiwan) was used for RNA extraction. The mRNA expression of
and
was measured by real-time polymerase chain reaction in PBMCs in preeclamptic and normal pregnancies.
A significant increase in mRNA expression of
and
(p
0.001) was observed in PBMCs of preeclamptic pregnancies compared to the control group (p
0.001). Moreover, a significant positive correlation was found between the
mRNA expression and
and
(p
0.001).
The results lead to the conclusion that mRNA expression of
and
in the maternal PBMCs is altered in preeclamptic pregnancies and might contribute to the pathogenesis of the disease. |
| doi_str_mv | 10.18502/ijrm.v13i4.6886 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7218673</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_00856a9b18b144088da0ce90417ecc44</doaj_id><sourcerecordid>2410350493</sourcerecordid><originalsourceid>FETCH-LOGICAL-d360t-e2870cec6ee83b53e57eda907a12182bc1122a33eed25804cdb9d31a4c91f98c3</originalsourceid><addsrcrecordid>eNpdkU1vEzEQhlcIRKvSOydkiQuXDf5cezkgVRGFSpW4wHk1a08SR951sHfT5t_jpAFRTv569Pidmap6y-iCGUX5R79Nw2LPhJeLxpjmRXXJpW5qoTV_ed5LRs1FdZ3zllLKGsUYF6-rC8Flq5lSl9XjMg47SDD5PZI1jkjwcZcwZx9HAiOEQ_aZxBW5hVzOjiQMMKE7sZn4kRQabYBhN3l7IjYIYdocyEMccPxEbohNMec6o52KFALJ0-wOb6pXKwgZr8_rVfXz9suP5bf6_vvXu-XNfe1EQ6caudHUom0QjeiVQKXRQUs1MM4M722piIMQiI4rQ6V1fesEA2lbtmqNFVfV3ZPXRdh2u-QHSIcugu9OFzGtO0glesCOUqMaaHtmeiYlNcZB-bqlkmm0Vsri-vzk2s39gM7iOCUIz6TPX0a_6dZx3-mStdGiCD6cBSn-mjFP3eCzxRBgxDjnjpd5CUVle0Tf_4du45xK-44UVY0yhh6pd_8m-hvlz4DFb7_0qyU</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2405658803</pqid></control><display><type>article</type><title>Comparative gene expression analysis of Fas and related genes in preeclamptic and healthy women: A cross-sectional study</title><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><creator>Ali, Zaima ; Khaliq, Saba ; Zaki, Saima ; Ahmad, Hafiz Usman ; Lone, Khalid Pervaiz</creator><creatorcontrib>Ali, Zaima ; Khaliq, Saba ; Zaki, Saima ; Ahmad, Hafiz Usman ; Lone, Khalid Pervaiz</creatorcontrib><description>Preeclampsia is a hypertensive disorder of pregnancy affecting about 2-10% pregnancies worldwide. mRNA expression of tumor necrosis factor alpha
and
have been reported to be altered in placental bed in preeclamptic pregnancies. We hypothesized that the expression of these genes is also altered in peripheral blood mononuclear cells (PBMCs) in preeclampsia.
To compare the expression of Fas receptor and related genes in PBMCs of preeclamptic and normotensive pregnant women.
A cross-sectional comparative study comprising of 18 cases and 18 controls was designed. 5 ml of venous blood was drawn and collected considering aseptic measures. Buffy coat was separated by centrifugation and stored at -20°C. Favor Prep total RNA Isolation Kit (Favorgen, Taiwan) was used for RNA extraction. The mRNA expression of
and
was measured by real-time polymerase chain reaction in PBMCs in preeclamptic and normal pregnancies.
A significant increase in mRNA expression of
and
(p
0.001) was observed in PBMCs of preeclamptic pregnancies compared to the control group (p
0.001). Moreover, a significant positive correlation was found between the
mRNA expression and
and
(p
0.001).
The results lead to the conclusion that mRNA expression of
and
in the maternal PBMCs is altered in preeclamptic pregnancies and might contribute to the pathogenesis of the disease.</description><identifier>ISSN: 2476-4108</identifier><identifier>EISSN: 2476-3772</identifier><identifier>DOI: 10.18502/ijrm.v13i4.6886</identifier><identifier>PMID: 32497155</identifier><language>eng</language><publisher>Iran: Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</publisher><subject>Apoptosis ; Cross-sectional studies ; Gene expression ; Preeclampsia ; preeclampsia, tnf- α , fas, apoptosis ; Pregnancy ; Tumor necrosis factor-TNF ; Womens health</subject><ispartof>International Journal of Reproductive BioMedicine, 2020-04, Vol.18 (4), p.235-242</ispartof><rights>Copyright © 2020 Ali et al.</rights><rights>2020. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2020 Ali et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218673/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218673/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32497155$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ali, Zaima</creatorcontrib><creatorcontrib>Khaliq, Saba</creatorcontrib><creatorcontrib>Zaki, Saima</creatorcontrib><creatorcontrib>Ahmad, Hafiz Usman</creatorcontrib><creatorcontrib>Lone, Khalid Pervaiz</creatorcontrib><title>Comparative gene expression analysis of Fas and related genes in preeclamptic and healthy women: A cross-sectional study</title><title>International Journal of Reproductive BioMedicine</title><addtitle>Int J Reprod Biomed</addtitle><description>Preeclampsia is a hypertensive disorder of pregnancy affecting about 2-10% pregnancies worldwide. mRNA expression of tumor necrosis factor alpha
and
have been reported to be altered in placental bed in preeclamptic pregnancies. We hypothesized that the expression of these genes is also altered in peripheral blood mononuclear cells (PBMCs) in preeclampsia.
To compare the expression of Fas receptor and related genes in PBMCs of preeclamptic and normotensive pregnant women.
A cross-sectional comparative study comprising of 18 cases and 18 controls was designed. 5 ml of venous blood was drawn and collected considering aseptic measures. Buffy coat was separated by centrifugation and stored at -20°C. Favor Prep total RNA Isolation Kit (Favorgen, Taiwan) was used for RNA extraction. The mRNA expression of
and
was measured by real-time polymerase chain reaction in PBMCs in preeclamptic and normal pregnancies.
A significant increase in mRNA expression of
and
(p
0.001) was observed in PBMCs of preeclamptic pregnancies compared to the control group (p
0.001). Moreover, a significant positive correlation was found between the
mRNA expression and
and
(p
0.001).
The results lead to the conclusion that mRNA expression of
and
in the maternal PBMCs is altered in preeclamptic pregnancies and might contribute to the pathogenesis of the disease.</description><subject>Apoptosis</subject><subject>Cross-sectional studies</subject><subject>Gene expression</subject><subject>Preeclampsia</subject><subject>preeclampsia, tnf- α , fas, apoptosis</subject><subject>Pregnancy</subject><subject>Tumor necrosis factor-TNF</subject><subject>Womens health</subject><issn>2476-4108</issn><issn>2476-3772</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>DOA</sourceid><recordid>eNpdkU1vEzEQhlcIRKvSOydkiQuXDf5cezkgVRGFSpW4wHk1a08SR951sHfT5t_jpAFRTv569Pidmap6y-iCGUX5R79Nw2LPhJeLxpjmRXXJpW5qoTV_ed5LRs1FdZ3zllLKGsUYF6-rC8Flq5lSl9XjMg47SDD5PZI1jkjwcZcwZx9HAiOEQ_aZxBW5hVzOjiQMMKE7sZn4kRQabYBhN3l7IjYIYdocyEMccPxEbohNMec6o52KFALJ0-wOb6pXKwgZr8_rVfXz9suP5bf6_vvXu-XNfe1EQ6caudHUom0QjeiVQKXRQUs1MM4M722piIMQiI4rQ6V1fesEA2lbtmqNFVfV3ZPXRdh2u-QHSIcugu9OFzGtO0glesCOUqMaaHtmeiYlNcZB-bqlkmm0Vsri-vzk2s39gM7iOCUIz6TPX0a_6dZx3-mStdGiCD6cBSn-mjFP3eCzxRBgxDjnjpd5CUVle0Tf_4du45xK-44UVY0yhh6pd_8m-hvlz4DFb7_0qyU</recordid><startdate>20200401</startdate><enddate>20200401</enddate><creator>Ali, Zaima</creator><creator>Khaliq, Saba</creator><creator>Zaki, Saima</creator><creator>Ahmad, Hafiz Usman</creator><creator>Lone, Khalid Pervaiz</creator><general>Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</general><general>Knowledge E</general><general>Shahid Sadoughi University of Medical Sciences</general><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20200401</creationdate><title>Comparative gene expression analysis of Fas and related genes in preeclamptic and healthy women: A cross-sectional study</title><author>Ali, Zaima ; Khaliq, Saba ; Zaki, Saima ; Ahmad, Hafiz Usman ; Lone, Khalid Pervaiz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d360t-e2870cec6ee83b53e57eda907a12182bc1122a33eed25804cdb9d31a4c91f98c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Apoptosis</topic><topic>Cross-sectional studies</topic><topic>Gene expression</topic><topic>Preeclampsia</topic><topic>preeclampsia, tnf- α , fas, apoptosis</topic><topic>Pregnancy</topic><topic>Tumor necrosis factor-TNF</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ali, Zaima</creatorcontrib><creatorcontrib>Khaliq, Saba</creatorcontrib><creatorcontrib>Zaki, Saima</creatorcontrib><creatorcontrib>Ahmad, Hafiz Usman</creatorcontrib><creatorcontrib>Lone, Khalid Pervaiz</creatorcontrib><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>International Journal of Reproductive BioMedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ali, Zaima</au><au>Khaliq, Saba</au><au>Zaki, Saima</au><au>Ahmad, Hafiz Usman</au><au>Lone, Khalid Pervaiz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative gene expression analysis of Fas and related genes in preeclamptic and healthy women: A cross-sectional study</atitle><jtitle>International Journal of Reproductive BioMedicine</jtitle><addtitle>Int J Reprod Biomed</addtitle><date>2020-04-01</date><risdate>2020</risdate><volume>18</volume><issue>4</issue><spage>235</spage><epage>242</epage><pages>235-242</pages><issn>2476-4108</issn><eissn>2476-3772</eissn><abstract>Preeclampsia is a hypertensive disorder of pregnancy affecting about 2-10% pregnancies worldwide. mRNA expression of tumor necrosis factor alpha
and
have been reported to be altered in placental bed in preeclamptic pregnancies. We hypothesized that the expression of these genes is also altered in peripheral blood mononuclear cells (PBMCs) in preeclampsia.
To compare the expression of Fas receptor and related genes in PBMCs of preeclamptic and normotensive pregnant women.
A cross-sectional comparative study comprising of 18 cases and 18 controls was designed. 5 ml of venous blood was drawn and collected considering aseptic measures. Buffy coat was separated by centrifugation and stored at -20°C. Favor Prep total RNA Isolation Kit (Favorgen, Taiwan) was used for RNA extraction. The mRNA expression of
and
was measured by real-time polymerase chain reaction in PBMCs in preeclamptic and normal pregnancies.
A significant increase in mRNA expression of
and
(p
0.001) was observed in PBMCs of preeclamptic pregnancies compared to the control group (p
0.001). Moreover, a significant positive correlation was found between the
mRNA expression and
and
(p
0.001).
The results lead to the conclusion that mRNA expression of
and
in the maternal PBMCs is altered in preeclamptic pregnancies and might contribute to the pathogenesis of the disease.</abstract><cop>Iran</cop><pub>Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</pub><pmid>32497155</pmid><doi>10.18502/ijrm.v13i4.6886</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | International Journal of Reproductive BioMedicine, 2020-04, Vol.18 (4), p.235-242 |
| issn | 2476-4108 2476-3772 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7218673 |
| source | DOAJ Directory of Open Access Journals; PubMed Central |
| subjects | Apoptosis Cross-sectional studies Gene expression Preeclampsia preeclampsia, tnf- α , fas, apoptosis Pregnancy Tumor necrosis factor-TNF Womens health |
| title | Comparative gene expression analysis of Fas and related genes in preeclamptic and healthy women: A cross-sectional study |
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