Inhibition of pathogenic Vibrio harveyi using calamenene, derived from the Indian gorgonian Subergorgia reticulata, and its synthetic analog
We report the synthesis and antimicrobial properties of a partially reduced dihydronathphthoquinone analogue of 2-methoxy, 5-acetoxy calamenene, extracted from Subergorgia reticulata . The growth of a pathogenic Vibrio harveyi strain was effectively controlled by the calamenene derivative 1 ( Cala1)...
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| Veröffentlicht in: | 3 Biotech 2020-06, Vol.10 (6), p.248-248, Article 248 |
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| creator | Limna Mol, V. P. Abdulaziz, Anas Sneha, K. G. Praveen, P. J. Raveendran, T. V. Parameswaran, P. S. |
| description | We report the synthesis and antimicrobial properties of a partially reduced dihydronathphthoquinone analogue of 2-methoxy, 5-acetoxy calamenene, extracted from
Subergorgia reticulata
. The growth of a pathogenic
Vibrio harveyi
strain was effectively controlled by the calamenene derivative
1
(
Cala1)
and its synthetic analog
2 (Cala2)
. Complete mortality of
V. harveyi
was observed with 2.5 and 0.5 µg mL
−1
concentrations of
Cala1 and Cala2
, respectively. The metabolic assays demonstrated that
Cala1
is a bacteriostatic agent while
Cala2
showed bactericidal properties. It was confirmed that translocation of
Cala2
into the cytoplasm does not induce any change to the integrity of the bacterial cell wall. The
Cala2
induced damage to the genetic material of 70% of cells while genetic material of 91% of cells treated with
Cala1
remained intact. The
Cala2
is, therefore, proposed as a potential bactericidal compound against the aquaculture pathogen
V. harveyi
. The fact that the
Cala2
exhibited minimal cytotoxicity to
Artemia
nauplii indicates its potential use as an antimicrobial agent for aquaculture operations. |
| doi_str_mv | 10.1007/s13205-020-02241-1 |
| format | Article |
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Subergorgia reticulata
. The growth of a pathogenic
Vibrio harveyi
strain was effectively controlled by the calamenene derivative
1
(
Cala1)
and its synthetic analog
2 (Cala2)
. Complete mortality of
V. harveyi
was observed with 2.5 and 0.5 µg mL
−1
concentrations of
Cala1 and Cala2
, respectively. The metabolic assays demonstrated that
Cala1
is a bacteriostatic agent while
Cala2
showed bactericidal properties. It was confirmed that translocation of
Cala2
into the cytoplasm does not induce any change to the integrity of the bacterial cell wall. The
Cala2
induced damage to the genetic material of 70% of cells while genetic material of 91% of cells treated with
Cala1
remained intact. The
Cala2
is, therefore, proposed as a potential bactericidal compound against the aquaculture pathogen
V. harveyi
. The fact that the
Cala2
exhibited minimal cytotoxicity to
Artemia
nauplii indicates its potential use as an antimicrobial agent for aquaculture operations.</description><identifier>ISSN: 2190-572X</identifier><identifier>EISSN: 2190-5738</identifier><identifier>DOI: 10.1007/s13205-020-02241-1</identifier><identifier>PMID: 32411572</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Agriculture ; Antiinfectives and antibacterials ; Antimicrobial agents ; Aquaculture ; Artemia ; Bacteria ; Bioinformatics ; Biomaterials ; Biotechnology ; Cancer Research ; Cell walls ; Chemistry ; Chemistry and Materials Science ; Cytoplasm ; Cytotoxicity ; Original ; Original Article ; Stem Cells ; Subergorgia reticulata ; Toxicity ; Vibrio harveyi</subject><ispartof>3 Biotech, 2020-06, Vol.10 (6), p.248-248, Article 248</ispartof><rights>King Abdulaziz City for Science and Technology 2020</rights><rights>King Abdulaziz City for Science and Technology 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-f58233b68102de12e22b1512e45947fcc7dc018b626912064742ed8e3ffef5c73</citedby><cites>FETCH-LOGICAL-c474t-f58233b68102de12e22b1512e45947fcc7dc018b626912064742ed8e3ffef5c73</cites><orcidid>0000-0002-1396-3645</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214568/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214568/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32411572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Limna Mol, V. P.</creatorcontrib><creatorcontrib>Abdulaziz, Anas</creatorcontrib><creatorcontrib>Sneha, K. G.</creatorcontrib><creatorcontrib>Praveen, P. J.</creatorcontrib><creatorcontrib>Raveendran, T. V.</creatorcontrib><creatorcontrib>Parameswaran, P. S.</creatorcontrib><title>Inhibition of pathogenic Vibrio harveyi using calamenene, derived from the Indian gorgonian Subergorgia reticulata, and its synthetic analog</title><title>3 Biotech</title><addtitle>3 Biotech</addtitle><addtitle>3 Biotech</addtitle><description>We report the synthesis and antimicrobial properties of a partially reduced dihydronathphthoquinone analogue of 2-methoxy, 5-acetoxy calamenene, extracted from
Subergorgia reticulata
. The growth of a pathogenic
Vibrio harveyi
strain was effectively controlled by the calamenene derivative
1
(
Cala1)
and its synthetic analog
2 (Cala2)
. Complete mortality of
V. harveyi
was observed with 2.5 and 0.5 µg mL
−1
concentrations of
Cala1 and Cala2
, respectively. The metabolic assays demonstrated that
Cala1
is a bacteriostatic agent while
Cala2
showed bactericidal properties. It was confirmed that translocation of
Cala2
into the cytoplasm does not induce any change to the integrity of the bacterial cell wall. The
Cala2
induced damage to the genetic material of 70% of cells while genetic material of 91% of cells treated with
Cala1
remained intact. The
Cala2
is, therefore, proposed as a potential bactericidal compound against the aquaculture pathogen
V. harveyi
. The fact that the
Cala2
exhibited minimal cytotoxicity to
Artemia
nauplii indicates its potential use as an antimicrobial agent for aquaculture operations.</description><subject>Agriculture</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Aquaculture</subject><subject>Artemia</subject><subject>Bacteria</subject><subject>Bioinformatics</subject><subject>Biomaterials</subject><subject>Biotechnology</subject><subject>Cancer Research</subject><subject>Cell walls</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cytoplasm</subject><subject>Cytotoxicity</subject><subject>Original</subject><subject>Original Article</subject><subject>Stem Cells</subject><subject>Subergorgia reticulata</subject><subject>Toxicity</subject><subject>Vibrio harveyi</subject><issn>2190-572X</issn><issn>2190-5738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9UcuKFDEUDaI4wzg_4EICblxMaZKq1GMjyOCjYcCFD9yFVOqmKkNV0iaphv4HP9rb02P7WJhLuDe555zkcgh5ytlLzljzKvFSMFkwwXCLihf8ATkXvGOFbMr24akW387IZUq3DJfksuPsMTkrkcCxd05-bPzkepdd8DRYutV5CiN4Z-hX10cX6KTjDvaOrsn5kRo96wU8xhUdILodDNTGsNA8Ad34wWlPxxDH4A_Vp7WHeDg6TSNkZ9ZZZ31FtR-oy4mmvUce3uONnsP4hDyyek5weZ8vyJd3bz9ffyhuPr7fXL-5KUzVVLmwshVl2dctZ2IALkCInkvMleyqxhrTDIbxtq9F3XHBaiQJGFoorQUrTVNekNdH3e3aLzAY8DnqWW2jW3Tcq6Cd-rvj3aTGsFON4JWsWxR4cS8Qw_cVUlaLSwbmWXsIa1KiYhhVxyRCn_8DvQ1rxHHvUFzgLB1HlDiiTAwpRbCnz3CmDn6ro98K_VZ3fqsD6dmfY5wov9xFQHkEJGz5EeLvt_8j-xOZELf6</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>Limna Mol, V. P.</creator><creator>Abdulaziz, Anas</creator><creator>Sneha, K. G.</creator><creator>Praveen, P. J.</creator><creator>Raveendran, T. V.</creator><creator>Parameswaran, P. S.</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1396-3645</orcidid></search><sort><creationdate>20200601</creationdate><title>Inhibition of pathogenic Vibrio harveyi using calamenene, derived from the Indian gorgonian Subergorgia reticulata, and its synthetic analog</title><author>Limna Mol, V. P. ; Abdulaziz, Anas ; Sneha, K. G. ; Praveen, P. J. ; Raveendran, T. V. ; Parameswaran, P. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-f58233b68102de12e22b1512e45947fcc7dc018b626912064742ed8e3ffef5c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Agriculture</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial agents</topic><topic>Aquaculture</topic><topic>Artemia</topic><topic>Bacteria</topic><topic>Bioinformatics</topic><topic>Biomaterials</topic><topic>Biotechnology</topic><topic>Cancer Research</topic><topic>Cell walls</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cytoplasm</topic><topic>Cytotoxicity</topic><topic>Original</topic><topic>Original Article</topic><topic>Stem Cells</topic><topic>Subergorgia reticulata</topic><topic>Toxicity</topic><topic>Vibrio harveyi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Limna Mol, V. P.</creatorcontrib><creatorcontrib>Abdulaziz, Anas</creatorcontrib><creatorcontrib>Sneha, K. G.</creatorcontrib><creatorcontrib>Praveen, P. J.</creatorcontrib><creatorcontrib>Raveendran, T. V.</creatorcontrib><creatorcontrib>Parameswaran, P. S.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>3 Biotech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Limna Mol, V. P.</au><au>Abdulaziz, Anas</au><au>Sneha, K. G.</au><au>Praveen, P. J.</au><au>Raveendran, T. V.</au><au>Parameswaran, P. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of pathogenic Vibrio harveyi using calamenene, derived from the Indian gorgonian Subergorgia reticulata, and its synthetic analog</atitle><jtitle>3 Biotech</jtitle><stitle>3 Biotech</stitle><addtitle>3 Biotech</addtitle><date>2020-06-01</date><risdate>2020</risdate><volume>10</volume><issue>6</issue><spage>248</spage><epage>248</epage><pages>248-248</pages><artnum>248</artnum><issn>2190-572X</issn><eissn>2190-5738</eissn><abstract>We report the synthesis and antimicrobial properties of a partially reduced dihydronathphthoquinone analogue of 2-methoxy, 5-acetoxy calamenene, extracted from
Subergorgia reticulata
. The growth of a pathogenic
Vibrio harveyi
strain was effectively controlled by the calamenene derivative
1
(
Cala1)
and its synthetic analog
2 (Cala2)
. Complete mortality of
V. harveyi
was observed with 2.5 and 0.5 µg mL
−1
concentrations of
Cala1 and Cala2
, respectively. The metabolic assays demonstrated that
Cala1
is a bacteriostatic agent while
Cala2
showed bactericidal properties. It was confirmed that translocation of
Cala2
into the cytoplasm does not induce any change to the integrity of the bacterial cell wall. The
Cala2
induced damage to the genetic material of 70% of cells while genetic material of 91% of cells treated with
Cala1
remained intact. The
Cala2
is, therefore, proposed as a potential bactericidal compound against the aquaculture pathogen
V. harveyi
. The fact that the
Cala2
exhibited minimal cytotoxicity to
Artemia
nauplii indicates its potential use as an antimicrobial agent for aquaculture operations.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>32411572</pmid><doi>10.1007/s13205-020-02241-1</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-1396-3645</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Springer Nature Link eJournals; PubMed Central; EZB Electronic Journals Library |
| subjects | Agriculture Antiinfectives and antibacterials Antimicrobial agents Aquaculture Artemia Bacteria Bioinformatics Biomaterials Biotechnology Cancer Research Cell walls Chemistry Chemistry and Materials Science Cytoplasm Cytotoxicity Original Original Article Stem Cells Subergorgia reticulata Toxicity Vibrio harveyi |
| title | Inhibition of pathogenic Vibrio harveyi using calamenene, derived from the Indian gorgonian Subergorgia reticulata, and its synthetic analog |
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