Long non‐coding RNA‐H19 stimulates osteogenic differentiation of bone marrow mesenchymal stem cells via the microRNA‐149/SDF‐1 axis
Bone defects resulting from non‐union fractures or tumour resections are common clinical problems. Long non‐coding RNAs (lncRNAs) are reported to play vital roles in stem cell differentiation. The aim of this study was to elucidate the role of lncRNA‐H19 in osteogenic differentiation of bone marrow...
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| creator | Li, Guangjie Yun, Xiangdong Ye, Kaishan Zhao, Haiyan An, Jiangdong Zhang, Xueliang Han, Xingwen Li, Yanhong Wang, Shuanke |
| description | Bone defects resulting from non‐union fractures or tumour resections are common clinical problems. Long non‐coding RNAs (lncRNAs) are reported to play vital roles in stem cell differentiation. The aim of this study was to elucidate the role of lncRNA‐H19 in osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs). Following the establishment of an osteogenic differentiation model in rats, the expression of H19, microRNA‐149 (miR‐149) and stromal cell‐derived factor‐1 (SDF‐1) was measured by RT‐qPCR. Thereafter, BMMSCs were isolated from rats and treated with a series of mimic, inhibitor or siRNA. SDF‐1 expression, alkaline phosphatase (ALP) activity and osteocalcin (OCN) content were detected. The mineralized and calcified nodules were assessed by alizarin red S and Von Kossa staining. BMMSC surface markers were detected by flow cytometry. Western blot analysis was used to measure the expression of ALP, OCN, runt‐related transcription factor 2 (RUNX2) and osterix (OSX) proteins. Lastly, dual‐luciferase reporter gene assay and RNA immunoprecipitation were applied to verify the relationship of H19, miR‐149 and SDF‐1. Overexpressed H19 and SDF‐1 and poorly expressed miR‐149 were found in rats with osteogenic differentiation. H19 increased SDF‐1 expression by binding to miR‐149. H19 enhanced ALP activity, OCN content, calcium deposit and ALP, OCN, RUNX2 and OSX protein expression of BMMSCS by up‐regulating SDF‐1 via binding to miR‐149. Taken together, up‐regulated H19 could promote the osteogenic differentiation of BMMSCs by increasing SDF‐1 via miR‐149. |
| doi_str_mv | 10.1111/jcmm.15040 |
| format | Article |
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Long non‐coding RNAs (lncRNAs) are reported to play vital roles in stem cell differentiation. The aim of this study was to elucidate the role of lncRNA‐H19 in osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs). Following the establishment of an osteogenic differentiation model in rats, the expression of H19, microRNA‐149 (miR‐149) and stromal cell‐derived factor‐1 (SDF‐1) was measured by RT‐qPCR. Thereafter, BMMSCs were isolated from rats and treated with a series of mimic, inhibitor or siRNA. SDF‐1 expression, alkaline phosphatase (ALP) activity and osteocalcin (OCN) content were detected. The mineralized and calcified nodules were assessed by alizarin red S and Von Kossa staining. BMMSC surface markers were detected by flow cytometry. Western blot analysis was used to measure the expression of ALP, OCN, runt‐related transcription factor 2 (RUNX2) and osterix (OSX) proteins. Lastly, dual‐luciferase reporter gene assay and RNA immunoprecipitation were applied to verify the relationship of H19, miR‐149 and SDF‐1. Overexpressed H19 and SDF‐1 and poorly expressed miR‐149 were found in rats with osteogenic differentiation. H19 increased SDF‐1 expression by binding to miR‐149. H19 enhanced ALP activity, OCN content, calcium deposit and ALP, OCN, RUNX2 and OSX protein expression of BMMSCS by up‐regulating SDF‐1 via binding to miR‐149. Taken together, up‐regulated H19 could promote the osteogenic differentiation of BMMSCs by increasing SDF‐1 via miR‐149.</description><identifier>ISSN: 1582-1838</identifier><identifier>ISSN: 1582-4934</identifier><identifier>EISSN: 1582-4934</identifier><identifier>DOI: 10.1111/jcmm.15040</identifier><identifier>PMID: 32198976</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Alkaline phosphatase ; Alkaline Phosphatase - metabolism ; Animals ; Bone cancer ; bone defects ; Bone marrow ; Bone Marrow Cells - cytology ; bone marrow mesenchymal stem cells ; Bone Regeneration ; Cbfa-1 protein ; Cell Differentiation ; Chemokine CXCL12 - metabolism ; Core Binding Factor Alpha 1 Subunit - metabolism ; Flow cytometry ; Fractures ; Gene Expression Regulation ; Genes, Reporter ; Immunoprecipitation ; Laboratory animals ; long non‐coding RNA‐H19 ; Male ; Mesenchymal stem cells ; Mesenchymal Stem Cells - cytology ; MicroRNAs ; MicroRNAs - metabolism ; microRNA‐149 ; miRNA ; Non-coding RNA ; Original ; Osteocalcin ; Osteocalcin - biosynthesis ; Osteogenesis ; osteogenic differentiation ; Polymethyl methacrylate ; Rats ; Rats, Sprague-Dawley ; Reporter gene ; RNA, Long Noncoding - metabolism ; siRNA ; Stem cell transplantation ; Stem cells ; stromal cell‐derived factor‐1 ; Surface markers ; Sutures ; Transcription Factors - metabolism ; Transfection ; Tumors ; Up-Regulation</subject><ispartof>Journal of cellular and molecular medicine, 2020-05, Vol.24 (9), p.4944-4955</ispartof><rights>2020 The Authors. published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.</rights><rights>2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.</rights><rights>2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4480-5207924e1fa1f9e2f072c13ea72e58cb9fbc85df87b19453e0092163903a00903</citedby><cites>FETCH-LOGICAL-c4480-5207924e1fa1f9e2f072c13ea72e58cb9fbc85df87b19453e0092163903a00903</cites><orcidid>0000-0002-0446-8327</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205807/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205807/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32198976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Guangjie</creatorcontrib><creatorcontrib>Yun, Xiangdong</creatorcontrib><creatorcontrib>Ye, Kaishan</creatorcontrib><creatorcontrib>Zhao, Haiyan</creatorcontrib><creatorcontrib>An, Jiangdong</creatorcontrib><creatorcontrib>Zhang, Xueliang</creatorcontrib><creatorcontrib>Han, Xingwen</creatorcontrib><creatorcontrib>Li, Yanhong</creatorcontrib><creatorcontrib>Wang, Shuanke</creatorcontrib><title>Long non‐coding RNA‐H19 stimulates osteogenic differentiation of bone marrow mesenchymal stem cells via the microRNA‐149/SDF‐1 axis</title><title>Journal of cellular and molecular medicine</title><addtitle>J Cell Mol Med</addtitle><description>Bone defects resulting from non‐union fractures or tumour resections are common clinical problems. Long non‐coding RNAs (lncRNAs) are reported to play vital roles in stem cell differentiation. The aim of this study was to elucidate the role of lncRNA‐H19 in osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs). Following the establishment of an osteogenic differentiation model in rats, the expression of H19, microRNA‐149 (miR‐149) and stromal cell‐derived factor‐1 (SDF‐1) was measured by RT‐qPCR. Thereafter, BMMSCs were isolated from rats and treated with a series of mimic, inhibitor or siRNA. SDF‐1 expression, alkaline phosphatase (ALP) activity and osteocalcin (OCN) content were detected. The mineralized and calcified nodules were assessed by alizarin red S and Von Kossa staining. BMMSC surface markers were detected by flow cytometry. Western blot analysis was used to measure the expression of ALP, OCN, runt‐related transcription factor 2 (RUNX2) and osterix (OSX) proteins. Lastly, dual‐luciferase reporter gene assay and RNA immunoprecipitation were applied to verify the relationship of H19, miR‐149 and SDF‐1. Overexpressed H19 and SDF‐1 and poorly expressed miR‐149 were found in rats with osteogenic differentiation. H19 increased SDF‐1 expression by binding to miR‐149. H19 enhanced ALP activity, OCN content, calcium deposit and ALP, OCN, RUNX2 and OSX protein expression of BMMSCS by up‐regulating SDF‐1 via binding to miR‐149. Taken together, up‐regulated H19 could promote the osteogenic differentiation of BMMSCs by increasing SDF‐1 via miR‐149.</description><subject>Alkaline phosphatase</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Bone cancer</subject><subject>bone defects</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - cytology</subject><subject>bone marrow mesenchymal stem cells</subject><subject>Bone Regeneration</subject><subject>Cbfa-1 protein</subject><subject>Cell Differentiation</subject><subject>Chemokine CXCL12 - metabolism</subject><subject>Core Binding Factor Alpha 1 Subunit - metabolism</subject><subject>Flow cytometry</subject><subject>Fractures</subject><subject>Gene Expression Regulation</subject><subject>Genes, Reporter</subject><subject>Immunoprecipitation</subject><subject>Laboratory animals</subject><subject>long non‐coding RNA‐H19</subject><subject>Male</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>MicroRNAs</subject><subject>MicroRNAs - metabolism</subject><subject>microRNA‐149</subject><subject>miRNA</subject><subject>Non-coding RNA</subject><subject>Original</subject><subject>Osteocalcin</subject><subject>Osteocalcin - biosynthesis</subject><subject>Osteogenesis</subject><subject>osteogenic differentiation</subject><subject>Polymethyl methacrylate</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reporter gene</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>siRNA</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>stromal cell‐derived factor‐1</subject><subject>Surface markers</subject><subject>Sutures</subject><subject>Transcription Factors - metabolism</subject><subject>Transfection</subject><subject>Tumors</subject><subject>Up-Regulation</subject><issn>1582-1838</issn><issn>1582-4934</issn><issn>1582-4934</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1u1DAUhSMEoqWw4QGQJTYIaVpfOz_2BqkaaEs1BYmfteV4rmc8Suw2Tlpmx55Nn5EnwWmGCljgje-VP517j0-WPQd6COkcbUzbHkJBc_og24dCsFkuef5wV4PgYi97EuOGUl4Cl4-zPc5AClmV-9mPRfAr4oP_-f3WhKVLzacPx6k5A0li79qh0T1GEmKPYYXeGbJ01mKHvne6d8GTYEkdPJJWd124IS1G9Ga9bXWTBLAlBpsmkmunSb9OlDNdmEZALo8-vz0ZK6K_ufg0e2R1E_HZ7j7Ivp68-zI_my0-nr6fHy9mJs8FnRWMVpLlCFaDlcgsrZgBjrpiWAhTS1sbUSytqGqQecGRUsmg5JJynUrKD7I3k-7lULe4NMlKpxt12blkYauCdurvF-_WahWuVcVoIWiVBF7tBLpwNWDsVeviaFN7DENUjIs0jwoYZ738B92EofPJnmIlCOBQSp6o1xOV_ibGDu39MkDVmLEaM1Z3GSf4xZ_r36O_Q00ATMCNa3D7Hyl1Pr-4mER_AQyJtY0</recordid><startdate>202005</startdate><enddate>202005</enddate><creator>Li, Guangjie</creator><creator>Yun, Xiangdong</creator><creator>Ye, Kaishan</creator><creator>Zhao, Haiyan</creator><creator>An, Jiangdong</creator><creator>Zhang, Xueliang</creator><creator>Han, Xingwen</creator><creator>Li, Yanhong</creator><creator>Wang, Shuanke</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0446-8327</orcidid></search><sort><creationdate>202005</creationdate><title>Long non‐coding RNA‐H19 stimulates osteogenic differentiation of bone marrow mesenchymal stem cells via the microRNA‐149/SDF‐1 axis</title><author>Li, Guangjie ; Yun, Xiangdong ; Ye, Kaishan ; Zhao, Haiyan ; An, Jiangdong ; Zhang, Xueliang ; Han, Xingwen ; Li, Yanhong ; Wang, Shuanke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4480-5207924e1fa1f9e2f072c13ea72e58cb9fbc85df87b19453e0092163903a00903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alkaline phosphatase</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Bone cancer</topic><topic>bone defects</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - cytology</topic><topic>bone marrow mesenchymal stem cells</topic><topic>Bone Regeneration</topic><topic>Cbfa-1 protein</topic><topic>Cell Differentiation</topic><topic>Chemokine CXCL12 - metabolism</topic><topic>Core Binding Factor Alpha 1 Subunit - metabolism</topic><topic>Flow cytometry</topic><topic>Fractures</topic><topic>Gene Expression Regulation</topic><topic>Genes, Reporter</topic><topic>Immunoprecipitation</topic><topic>Laboratory animals</topic><topic>long non‐coding RNA‐H19</topic><topic>Male</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>MicroRNAs</topic><topic>MicroRNAs - metabolism</topic><topic>microRNA‐149</topic><topic>miRNA</topic><topic>Non-coding RNA</topic><topic>Original</topic><topic>Osteocalcin</topic><topic>Osteocalcin - biosynthesis</topic><topic>Osteogenesis</topic><topic>osteogenic differentiation</topic><topic>Polymethyl methacrylate</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reporter gene</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>siRNA</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>stromal cell‐derived factor‐1</topic><topic>Surface markers</topic><topic>Sutures</topic><topic>Transcription Factors - metabolism</topic><topic>Transfection</topic><topic>Tumors</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Guangjie</creatorcontrib><creatorcontrib>Yun, Xiangdong</creatorcontrib><creatorcontrib>Ye, Kaishan</creatorcontrib><creatorcontrib>Zhao, Haiyan</creatorcontrib><creatorcontrib>An, Jiangdong</creatorcontrib><creatorcontrib>Zhang, Xueliang</creatorcontrib><creatorcontrib>Han, Xingwen</creatorcontrib><creatorcontrib>Li, Yanhong</creatorcontrib><creatorcontrib>Wang, Shuanke</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of cellular and molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Guangjie</au><au>Yun, Xiangdong</au><au>Ye, Kaishan</au><au>Zhao, Haiyan</au><au>An, Jiangdong</au><au>Zhang, Xueliang</au><au>Han, Xingwen</au><au>Li, Yanhong</au><au>Wang, Shuanke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long non‐coding RNA‐H19 stimulates osteogenic differentiation of bone marrow mesenchymal stem cells via the microRNA‐149/SDF‐1 axis</atitle><jtitle>Journal of cellular and molecular medicine</jtitle><addtitle>J Cell Mol Med</addtitle><date>2020-05</date><risdate>2020</risdate><volume>24</volume><issue>9</issue><spage>4944</spage><epage>4955</epage><pages>4944-4955</pages><issn>1582-1838</issn><issn>1582-4934</issn><eissn>1582-4934</eissn><abstract>Bone defects resulting from non‐union fractures or tumour resections are common clinical problems. Long non‐coding RNAs (lncRNAs) are reported to play vital roles in stem cell differentiation. The aim of this study was to elucidate the role of lncRNA‐H19 in osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs). Following the establishment of an osteogenic differentiation model in rats, the expression of H19, microRNA‐149 (miR‐149) and stromal cell‐derived factor‐1 (SDF‐1) was measured by RT‐qPCR. Thereafter, BMMSCs were isolated from rats and treated with a series of mimic, inhibitor or siRNA. SDF‐1 expression, alkaline phosphatase (ALP) activity and osteocalcin (OCN) content were detected. The mineralized and calcified nodules were assessed by alizarin red S and Von Kossa staining. BMMSC surface markers were detected by flow cytometry. Western blot analysis was used to measure the expression of ALP, OCN, runt‐related transcription factor 2 (RUNX2) and osterix (OSX) proteins. Lastly, dual‐luciferase reporter gene assay and RNA immunoprecipitation were applied to verify the relationship of H19, miR‐149 and SDF‐1. Overexpressed H19 and SDF‐1 and poorly expressed miR‐149 were found in rats with osteogenic differentiation. H19 increased SDF‐1 expression by binding to miR‐149. H19 enhanced ALP activity, OCN content, calcium deposit and ALP, OCN, RUNX2 and OSX protein expression of BMMSCS by up‐regulating SDF‐1 via binding to miR‐149. Taken together, up‐regulated H19 could promote the osteogenic differentiation of BMMSCs by increasing SDF‐1 via miR‐149.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>32198976</pmid><doi>10.1111/jcmm.15040</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-0446-8327</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Alkaline phosphatase Alkaline Phosphatase - metabolism Animals Bone cancer bone defects Bone marrow Bone Marrow Cells - cytology bone marrow mesenchymal stem cells Bone Regeneration Cbfa-1 protein Cell Differentiation Chemokine CXCL12 - metabolism Core Binding Factor Alpha 1 Subunit - metabolism Flow cytometry Fractures Gene Expression Regulation Genes, Reporter Immunoprecipitation Laboratory animals long non‐coding RNA‐H19 Male Mesenchymal stem cells Mesenchymal Stem Cells - cytology MicroRNAs MicroRNAs - metabolism microRNA‐149 miRNA Non-coding RNA Original Osteocalcin Osteocalcin - biosynthesis Osteogenesis osteogenic differentiation Polymethyl methacrylate Rats Rats, Sprague-Dawley Reporter gene RNA, Long Noncoding - metabolism siRNA Stem cell transplantation Stem cells stromal cell‐derived factor‐1 Surface markers Sutures Transcription Factors - metabolism Transfection Tumors Up-Regulation |
| title | Long non‐coding RNA‐H19 stimulates osteogenic differentiation of bone marrow mesenchymal stem cells via the microRNA‐149/SDF‐1 axis |
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