SARS-CoV-2 detection by direct rRT-PCR without RNA extraction

Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar® SARS-CoV-2 rRT-PCR kit (Altona Diagnostics). F...

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Veröffentlicht in:	Journal of clinical virology 2020-07, Vol.128, p.104423-104423, Article 104423
Hauptverfasser:	Merindol, Natacha, Pépin, Geneviève, Marchand, Caroline, Rheault, Marylène, Peterson, Christine, Poirier, André, Houle, Claudia, Germain, Hugo, Danylo, Alexis
Format:	Artikel
Sprache:	eng
Schlagworte:	Betacoronavirus - genetics Betacoronavirus - isolation & purification Coronavirus Coronavirus Infections - diagnosis Coronavirus Infections - virology COVID-19 COVID19 Direct rRTPCR Humans Nasopharynx - virology Pandemics Pneumonia, Viral - diagnosis Pneumonia, Viral - virology Reverse Transcriptase Polymerase Chain Reaction - methods RNA extraction RNA, Viral - genetics SARS-CoV-2 Specimen Handling Virus detection
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