CRISPR/Cas9-Directed Gene-Editing for the generation of loss-of–function mutants in high-throughput zebrafish FO screens

The ability to perform reverse genetics in the zebrafish model organism has been greatly advanced with the advent of the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated) system. The high level of efficiency in generating mutations when using the CRISPR/Cas9...

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Veröffentlicht in:	Current protocols in molecular biology (Print) 2017-07, Vol.119, p.31.9.1-31.9.22
Hauptverfasser:	Shankaran, Sunita, Dahlem, Timothy, Bisgrove, Brent W., Yost, H. Joseph, Tristani-Firouzi, Martin
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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