Effect of Sleeve Gastrectomy on Bone Metabolism and Serum 5-Hydroxytryptamine in Obese Rats
BACKGROUND Studies have shown that bariatric surgery, such as sleeve gastrectomy (SG), has an adverse effect on bone, including decreased bone mineral density (BMD) and bone metabolism. Peripheral 5-hydroxytryptamine (5-HT) has an adverse regulatory effect on bone formation. Here, we assessed change...
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| Veröffentlicht in: | Medical science monitor 2020-04, Vol.26, p.e924097-e924097 |
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| creator | Xu, Kai Ma, Junxing Liu, Zhi Wang, Xiaoyang Yan, Shaohua Liu, Zitian Sun, Fuyun Wang, Kexin |
| description | BACKGROUND Studies have shown that bariatric surgery, such as sleeve gastrectomy (SG), has an adverse effect on bone, including decreased bone mineral density (BMD) and bone metabolism. Peripheral 5-hydroxytryptamine (5-HT) has an adverse regulatory effect on bone formation. Here, we assessed changes in bone metabolism and whether 5-HT is involved in the effect of SG on bone metabolism. MATERIAL AND METHODS A rat model of obesity was established using Wistar rats. After successful modeling, rats were randomly assigned to 2 groups - the SG group and the Sham group - with 10 rats in each group. We then performed sleeve gastrectomy or sham operation. Bone metabolic markers and BMD of rats were measured at 2 and 16 weeks after the operation and the level of 5-HT in serum was determined. Rats were killed at 16 weeks after the operation, and bones of the hind limbs were harvested to measure 5-HT by immunofluorescence. RESULTS BMD was decreased and bone metabolism demonstrated a trend of bone destruction in the rats after SG. A significantly increasing trend in the level of serum 5-HT was found, and bone immunofluorescence showed increased expression of 5-HT. CONCLUSIONS BMD was decrease and bone metabolism demonstrated a trend of bone destruction after SG. SG can affect the level of 5-HT in serum or bone tissue and the 5-HT may be involved in the process through which SG affects bone metabolism. |
| doi_str_mv | 10.12659/MSM.924097 |
| format | Article |
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Peripheral 5-hydroxytryptamine (5-HT) has an adverse regulatory effect on bone formation. Here, we assessed changes in bone metabolism and whether 5-HT is involved in the effect of SG on bone metabolism. MATERIAL AND METHODS A rat model of obesity was established using Wistar rats. After successful modeling, rats were randomly assigned to 2 groups - the SG group and the Sham group - with 10 rats in each group. We then performed sleeve gastrectomy or sham operation. Bone metabolic markers and BMD of rats were measured at 2 and 16 weeks after the operation and the level of 5-HT in serum was determined. Rats were killed at 16 weeks after the operation, and bones of the hind limbs were harvested to measure 5-HT by immunofluorescence. RESULTS BMD was decreased and bone metabolism demonstrated a trend of bone destruction in the rats after SG. A significantly increasing trend in the level of serum 5-HT was found, and bone immunofluorescence showed increased expression of 5-HT. CONCLUSIONS BMD was decrease and bone metabolism demonstrated a trend of bone destruction after SG. SG can affect the level of 5-HT in serum or bone tissue and the 5-HT may be involved in the process through which SG affects bone metabolism.</description><identifier>ISSN: 1643-3750</identifier><identifier>ISSN: 1234-1010</identifier><identifier>EISSN: 1643-3750</identifier><identifier>DOI: 10.12659/MSM.924097</identifier><identifier>PMID: 32324718</identifier><language>eng</language><publisher>United States: International Scientific Literature, Inc</publisher><subject>Animal Study ; Animals ; Bariatric Surgery - adverse effects ; Bariatric Surgery - methods ; Bone and Bones - metabolism ; Diabetes Mellitus, Experimental - metabolism ; Diabetes Mellitus, Type 2 - blood ; Gastrectomy - adverse effects ; Gastrectomy - methods ; Male ; Obesity - metabolism ; Obesity - surgery ; Obesity, Morbid - metabolism ; Obesity, Morbid - surgery ; Rats ; Rats, Wistar ; Serotonin - analysis ; Serotonin - blood ; Weight Loss</subject><ispartof>Medical science monitor, 2020-04, Vol.26, p.e924097-e924097</ispartof><rights>Med Sci Monit, 2020 2020</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-cf334f018122201b02b893b615a3398172ddc7a76caae695b7a51534ea4e58d73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193245/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193245/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32324718$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Kai</creatorcontrib><creatorcontrib>Ma, Junxing</creatorcontrib><creatorcontrib>Liu, Zhi</creatorcontrib><creatorcontrib>Wang, Xiaoyang</creatorcontrib><creatorcontrib>Yan, Shaohua</creatorcontrib><creatorcontrib>Liu, Zitian</creatorcontrib><creatorcontrib>Sun, Fuyun</creatorcontrib><creatorcontrib>Wang, Kexin</creatorcontrib><title>Effect of Sleeve Gastrectomy on Bone Metabolism and Serum 5-Hydroxytryptamine in Obese Rats</title><title>Medical science monitor</title><addtitle>Med Sci Monit</addtitle><description>BACKGROUND Studies have shown that bariatric surgery, such as sleeve gastrectomy (SG), has an adverse effect on bone, including decreased bone mineral density (BMD) and bone metabolism. Peripheral 5-hydroxytryptamine (5-HT) has an adverse regulatory effect on bone formation. Here, we assessed changes in bone metabolism and whether 5-HT is involved in the effect of SG on bone metabolism. MATERIAL AND METHODS A rat model of obesity was established using Wistar rats. After successful modeling, rats were randomly assigned to 2 groups - the SG group and the Sham group - with 10 rats in each group. We then performed sleeve gastrectomy or sham operation. Bone metabolic markers and BMD of rats were measured at 2 and 16 weeks after the operation and the level of 5-HT in serum was determined. Rats were killed at 16 weeks after the operation, and bones of the hind limbs were harvested to measure 5-HT by immunofluorescence. RESULTS BMD was decreased and bone metabolism demonstrated a trend of bone destruction in the rats after SG. A significantly increasing trend in the level of serum 5-HT was found, and bone immunofluorescence showed increased expression of 5-HT. CONCLUSIONS BMD was decrease and bone metabolism demonstrated a trend of bone destruction after SG. SG can affect the level of 5-HT in serum or bone tissue and the 5-HT may be involved in the process through which SG affects bone metabolism.</description><subject>Animal Study</subject><subject>Animals</subject><subject>Bariatric Surgery - adverse effects</subject><subject>Bariatric Surgery - methods</subject><subject>Bone and Bones - metabolism</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Diabetes Mellitus, Type 2 - blood</subject><subject>Gastrectomy - adverse effects</subject><subject>Gastrectomy - methods</subject><subject>Male</subject><subject>Obesity - metabolism</subject><subject>Obesity - surgery</subject><subject>Obesity, Morbid - metabolism</subject><subject>Obesity, Morbid - surgery</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Serotonin - analysis</subject><subject>Serotonin - blood</subject><subject>Weight Loss</subject><issn>1643-3750</issn><issn>1234-1010</issn><issn>1643-3750</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1LxDAQxYMofp-8S46CdM1n01wElXVX2EVw9eQhpO1UK22zJlmx_73FVVlPM8z8eDO8h9AJJSPKUqkv5ov5SDNBtNpC-zQVPOFKku2Nfg8dhPBGCMtSInfRHmecCUWzffQ8riooInYVXjQAH4AnNkQ_jFzbY9fha9cBnkO0uWvq0GLblXgBftVimUz70rvPPvp-GW1bD2Dd4fscAuAHG8MR2qlsE-D4px6ip9vx4800md1P7m6uZkkhhIpJUXEuKkIzyhgjNCcszzTPUyot5zqjipVloaxKC2sh1TJXVlLJBVgBMisVP0SXa93lKm-hLKCL3jZm6evW-t44W5v_m65-NS_uwyiqBx_kIHD2I-Dd-wpCNG0dCmga24FbBcO4Fpkm9Bs9X6OFdyF4qP7OUGK-4zBDHGYdx0Cfbn72x_76z78AFCqFzQ</recordid><startdate>20200423</startdate><enddate>20200423</enddate><creator>Xu, Kai</creator><creator>Ma, Junxing</creator><creator>Liu, Zhi</creator><creator>Wang, Xiaoyang</creator><creator>Yan, Shaohua</creator><creator>Liu, Zitian</creator><creator>Sun, Fuyun</creator><creator>Wang, Kexin</creator><general>International Scientific Literature, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200423</creationdate><title>Effect of Sleeve Gastrectomy on Bone Metabolism and Serum 5-Hydroxytryptamine in Obese Rats</title><author>Xu, Kai ; Ma, Junxing ; Liu, Zhi ; Wang, Xiaoyang ; Yan, Shaohua ; Liu, Zitian ; Sun, Fuyun ; Wang, Kexin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-cf334f018122201b02b893b615a3398172ddc7a76caae695b7a51534ea4e58d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animal Study</topic><topic>Animals</topic><topic>Bariatric Surgery - adverse effects</topic><topic>Bariatric Surgery - methods</topic><topic>Bone and Bones - metabolism</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Diabetes Mellitus, Type 2 - blood</topic><topic>Gastrectomy - adverse effects</topic><topic>Gastrectomy - methods</topic><topic>Male</topic><topic>Obesity - metabolism</topic><topic>Obesity - surgery</topic><topic>Obesity, Morbid - metabolism</topic><topic>Obesity, Morbid - surgery</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Serotonin - analysis</topic><topic>Serotonin - blood</topic><topic>Weight Loss</topic><toplevel>online_resources</toplevel><creatorcontrib>Xu, Kai</creatorcontrib><creatorcontrib>Ma, Junxing</creatorcontrib><creatorcontrib>Liu, Zhi</creatorcontrib><creatorcontrib>Wang, Xiaoyang</creatorcontrib><creatorcontrib>Yan, Shaohua</creatorcontrib><creatorcontrib>Liu, Zitian</creatorcontrib><creatorcontrib>Sun, Fuyun</creatorcontrib><creatorcontrib>Wang, Kexin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Medical science monitor</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Kai</au><au>Ma, Junxing</au><au>Liu, Zhi</au><au>Wang, Xiaoyang</au><au>Yan, Shaohua</au><au>Liu, Zitian</au><au>Sun, Fuyun</au><au>Wang, Kexin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Sleeve Gastrectomy on Bone Metabolism and Serum 5-Hydroxytryptamine in Obese Rats</atitle><jtitle>Medical science monitor</jtitle><addtitle>Med Sci Monit</addtitle><date>2020-04-23</date><risdate>2020</risdate><volume>26</volume><spage>e924097</spage><epage>e924097</epage><pages>e924097-e924097</pages><issn>1643-3750</issn><issn>1234-1010</issn><eissn>1643-3750</eissn><abstract>BACKGROUND Studies have shown that bariatric surgery, such as sleeve gastrectomy (SG), has an adverse effect on bone, including decreased bone mineral density (BMD) and bone metabolism. Peripheral 5-hydroxytryptamine (5-HT) has an adverse regulatory effect on bone formation. Here, we assessed changes in bone metabolism and whether 5-HT is involved in the effect of SG on bone metabolism. MATERIAL AND METHODS A rat model of obesity was established using Wistar rats. After successful modeling, rats were randomly assigned to 2 groups - the SG group and the Sham group - with 10 rats in each group. We then performed sleeve gastrectomy or sham operation. Bone metabolic markers and BMD of rats were measured at 2 and 16 weeks after the operation and the level of 5-HT in serum was determined. Rats were killed at 16 weeks after the operation, and bones of the hind limbs were harvested to measure 5-HT by immunofluorescence. RESULTS BMD was decreased and bone metabolism demonstrated a trend of bone destruction in the rats after SG. A significantly increasing trend in the level of serum 5-HT was found, and bone immunofluorescence showed increased expression of 5-HT. CONCLUSIONS BMD was decrease and bone metabolism demonstrated a trend of bone destruction after SG. SG can affect the level of 5-HT in serum or bone tissue and the 5-HT may be involved in the process through which SG affects bone metabolism.</abstract><cop>United States</cop><pub>International Scientific Literature, Inc</pub><pmid>32324718</pmid><doi>10.12659/MSM.924097</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Animal Study Animals Bariatric Surgery - adverse effects Bariatric Surgery - methods Bone and Bones - metabolism Diabetes Mellitus, Experimental - metabolism Diabetes Mellitus, Type 2 - blood Gastrectomy - adverse effects Gastrectomy - methods Male Obesity - metabolism Obesity - surgery Obesity, Morbid - metabolism Obesity, Morbid - surgery Rats Rats, Wistar Serotonin - analysis Serotonin - blood Weight Loss |
| title | Effect of Sleeve Gastrectomy on Bone Metabolism and Serum 5-Hydroxytryptamine in Obese Rats |
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