Integrating Pharmacology and Microbial Network Analysis with Experimental Validation to Reveal the Mechanism of Composite Sophora Colon-Soluble Capsule against Ulcerative Colitis
Ulcerative colitis (UC) has multifactorial pathogenesis that acts synergistically, such as immune system dysregulation and expansion of infectious gut microbiota. Therefore, a multicomponent treatment derived from Chinese herbal medicine that interacts with multiple targets synergistically is needed...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2020, Vol.2020 (2020), p.1-16 |
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| creator | Tong, Zhanqi Wang, Shida Feng, Yang Gao, Lu Wang, Qiying Chen, Mingjun Ding, Yuxuan Song, Zitian |
| description | Ulcerative colitis (UC) has multifactorial pathogenesis that acts synergistically, such as immune system dysregulation and expansion of infectious gut microbiota. Therefore, a multicomponent treatment derived from Chinese herbal medicine that interacts with multiple targets synergistically is needed. Composite sophora colon-soluble capsule (CSCC) is a Chinese herbal formula that has shown therapeutic efficacy against UC in randomized clinical trials. However, its bioactive components and potential target genes against UC remain unclear. Here, we used a network pharmacology approach to detect component-target-pathway interactions of CSCC against UC. A total of 29 gene targets, 91 bioactive components, and 20 enriched pathways of CSCC were identified. The IL-17 signaling pathway activated by infectious gastrointestinal microbes and predicted by the network analysis to be a major pathway modulated by CSCC against UC was studied in a dextran sulfate sodium-induced colitis model. CSCC showed remarkable efficacy against UC with respect to the attenuation of colon length, body weight loss, and disease activity index through gut microbiota recovery and intestinal immune homeostasis. The rectal administration of CSCC reduced the numbers of Th17 cells isolated from both mesenteric lymph nodes and lamina propria mononuclear cells and the levels of IL-17A, IL-6, IL-1β, and TNF-α. Additionally, the percentage of Treg cells and the levels of their hallmark cytokines were upregulated. Rectal administration of CSCC led to microbiota regulation with a significant correlation between suppression of Verrucomicrobiaceae and Ruminococcaceae, as well as the elevation of Lactobacillaceae, and CSCC administration via microbiome correlation heatmaps and cooccurrence network analysis at multiple time points. Thus, our study presents an effective herbal formula, CSCC, for UC treatment and explores its components and mechanisms of efficacy through the examination of gut microbiota and hallmark cytokines in the IL-17 pathway. |
| doi_str_mv | 10.1155/2020/9521073 |
| format | Article |
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Therefore, a multicomponent treatment derived from Chinese herbal medicine that interacts with multiple targets synergistically is needed. Composite sophora colon-soluble capsule (CSCC) is a Chinese herbal formula that has shown therapeutic efficacy against UC in randomized clinical trials. However, its bioactive components and potential target genes against UC remain unclear. Here, we used a network pharmacology approach to detect component-target-pathway interactions of CSCC against UC. A total of 29 gene targets, 91 bioactive components, and 20 enriched pathways of CSCC were identified. The IL-17 signaling pathway activated by infectious gastrointestinal microbes and predicted by the network analysis to be a major pathway modulated by CSCC against UC was studied in a dextran sulfate sodium-induced colitis model. CSCC showed remarkable efficacy against UC with respect to the attenuation of colon length, body weight loss, and disease activity index through gut microbiota recovery and intestinal immune homeostasis. The rectal administration of CSCC reduced the numbers of Th17 cells isolated from both mesenteric lymph nodes and lamina propria mononuclear cells and the levels of IL-17A, IL-6, IL-1β, and TNF-α. Additionally, the percentage of Treg cells and the levels of their hallmark cytokines were upregulated. Rectal administration of CSCC led to microbiota regulation with a significant correlation between suppression of Verrucomicrobiaceae and Ruminococcaceae, as well as the elevation of Lactobacillaceae, and CSCC administration via microbiome correlation heatmaps and cooccurrence network analysis at multiple time points. Thus, our study presents an effective herbal formula, CSCC, for UC treatment and explores its components and mechanisms of efficacy through the examination of gut microbiota and hallmark cytokines in the IL-17 pathway.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2020/9521073</identifier><identifier>PMID: 32382313</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Alternative medicine ; Body weight ; Body weight loss ; Clinical trials ; Colon ; Cytokines ; Dextran ; Dextran sulfate ; Digestive system ; Flow cytometry ; Gastrointestinal tract ; Genes ; Health aspects ; Helper cells ; Herbal medicine ; Homeostasis ; IL-1β ; Immune system ; Immunoglobulins ; Inflammatory bowel disease ; Interleukin 17 ; Interleukin 6 ; Intestinal microflora ; Intestine ; Laboratory animals ; Lamina propria ; Leukocytes (mononuclear) ; Lymph nodes ; Lymphocytes T ; Medicine, Botanic ; Medicine, Herbal ; Microbiota ; Microbiota (Symbiotic organisms) ; Ontology ; Pharmacology ; Polysaccharides ; Proteins ; Rectum ; Signal transduction ; Software ; Tumor necrosis factor-α ; Ulcerative colitis</subject><ispartof>Evidence-based complementary and alternative medicine, 2020, Vol.2020 (2020), p.1-16</ispartof><rights>Copyright © 2020 Yuxuan Ding et al.</rights><rights>COPYRIGHT 2020 John Wiley & Sons, Inc.</rights><rights>Copyright © 2020 Yuxuan Ding et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 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Therefore, a multicomponent treatment derived from Chinese herbal medicine that interacts with multiple targets synergistically is needed. Composite sophora colon-soluble capsule (CSCC) is a Chinese herbal formula that has shown therapeutic efficacy against UC in randomized clinical trials. However, its bioactive components and potential target genes against UC remain unclear. Here, we used a network pharmacology approach to detect component-target-pathway interactions of CSCC against UC. A total of 29 gene targets, 91 bioactive components, and 20 enriched pathways of CSCC were identified. The IL-17 signaling pathway activated by infectious gastrointestinal microbes and predicted by the network analysis to be a major pathway modulated by CSCC against UC was studied in a dextran sulfate sodium-induced colitis model. CSCC showed remarkable efficacy against UC with respect to the attenuation of colon length, body weight loss, and disease activity index through gut microbiota recovery and intestinal immune homeostasis. The rectal administration of CSCC reduced the numbers of Th17 cells isolated from both mesenteric lymph nodes and lamina propria mononuclear cells and the levels of IL-17A, IL-6, IL-1β, and TNF-α. Additionally, the percentage of Treg cells and the levels of their hallmark cytokines were upregulated. Rectal administration of CSCC led to microbiota regulation with a significant correlation between suppression of Verrucomicrobiaceae and Ruminococcaceae, as well as the elevation of Lactobacillaceae, and CSCC administration via microbiome correlation heatmaps and cooccurrence network analysis at multiple time points. Thus, our study presents an effective herbal formula, CSCC, for UC treatment and explores its components and mechanisms of efficacy through the examination of gut microbiota and hallmark cytokines in the IL-17 pathway.</description><subject>Alternative medicine</subject><subject>Body weight</subject><subject>Body weight loss</subject><subject>Clinical trials</subject><subject>Colon</subject><subject>Cytokines</subject><subject>Dextran</subject><subject>Dextran sulfate</subject><subject>Digestive system</subject><subject>Flow cytometry</subject><subject>Gastrointestinal tract</subject><subject>Genes</subject><subject>Health aspects</subject><subject>Helper cells</subject><subject>Herbal medicine</subject><subject>Homeostasis</subject><subject>IL-1β</subject><subject>Immune system</subject><subject>Immunoglobulins</subject><subject>Inflammatory bowel disease</subject><subject>Interleukin 17</subject><subject>Interleukin 6</subject><subject>Intestinal microflora</subject><subject>Intestine</subject><subject>Laboratory animals</subject><subject>Lamina propria</subject><subject>Leukocytes (mononuclear)</subject><subject>Lymph nodes</subject><subject>Lymphocytes T</subject><subject>Medicine, Botanic</subject><subject>Medicine, Herbal</subject><subject>Microbiota</subject><subject>Microbiota (Symbiotic organisms)</subject><subject>Ontology</subject><subject>Pharmacology</subject><subject>Polysaccharides</subject><subject>Proteins</subject><subject>Rectum</subject><subject>Signal transduction</subject><subject>Software</subject><subject>Tumor necrosis factor-α</subject><subject>Ulcerative 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Pharmacology and Microbial Network Analysis with Experimental Validation to Reveal the Mechanism of Composite Sophora Colon-Soluble Capsule against Ulcerative Colitis</title><author>Tong, Zhanqi ; Wang, Shida ; Feng, Yang ; Gao, Lu ; Wang, Qiying ; Chen, Mingjun ; Ding, Yuxuan ; Song, Zitian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-6c4fbd92395736041ada57df871f3780f837e7d3ff7fc3bdcdaa0c3fb57ad09f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alternative medicine</topic><topic>Body weight</topic><topic>Body weight loss</topic><topic>Clinical trials</topic><topic>Colon</topic><topic>Cytokines</topic><topic>Dextran</topic><topic>Dextran sulfate</topic><topic>Digestive system</topic><topic>Flow cytometry</topic><topic>Gastrointestinal tract</topic><topic>Genes</topic><topic>Health aspects</topic><topic>Helper cells</topic><topic>Herbal medicine</topic><topic>Homeostasis</topic><topic>IL-1β</topic><topic>Immune system</topic><topic>Immunoglobulins</topic><topic>Inflammatory bowel disease</topic><topic>Interleukin 17</topic><topic>Interleukin 6</topic><topic>Intestinal microflora</topic><topic>Intestine</topic><topic>Laboratory animals</topic><topic>Lamina propria</topic><topic>Leukocytes (mononuclear)</topic><topic>Lymph nodes</topic><topic>Lymphocytes T</topic><topic>Medicine, Botanic</topic><topic>Medicine, Herbal</topic><topic>Microbiota</topic><topic>Microbiota (Symbiotic organisms)</topic><topic>Ontology</topic><topic>Pharmacology</topic><topic>Polysaccharides</topic><topic>Proteins</topic><topic>Rectum</topic><topic>Signal transduction</topic><topic>Software</topic><topic>Tumor necrosis factor-α</topic><topic>Ulcerative colitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tong, Zhanqi</creatorcontrib><creatorcontrib>Wang, 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medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tong, Zhanqi</au><au>Wang, Shida</au><au>Feng, Yang</au><au>Gao, Lu</au><au>Wang, Qiying</au><au>Chen, Mingjun</au><au>Ding, Yuxuan</au><au>Song, Zitian</au><au>Kang, Wen-yi</au><au>Wen-yi Kang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrating Pharmacology and Microbial Network Analysis with Experimental Validation to Reveal the Mechanism of Composite Sophora Colon-Soluble Capsule against Ulcerative Colitis</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2020</date><risdate>2020</risdate><volume>2020</volume><issue>2020</issue><spage>1</spage><epage>16</epage><pages>1-16</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Ulcerative colitis (UC) has multifactorial pathogenesis that acts synergistically, such as immune system dysregulation and expansion of infectious gut microbiota. Therefore, a multicomponent treatment derived from Chinese herbal medicine that interacts with multiple targets synergistically is needed. Composite sophora colon-soluble capsule (CSCC) is a Chinese herbal formula that has shown therapeutic efficacy against UC in randomized clinical trials. However, its bioactive components and potential target genes against UC remain unclear. Here, we used a network pharmacology approach to detect component-target-pathway interactions of CSCC against UC. A total of 29 gene targets, 91 bioactive components, and 20 enriched pathways of CSCC were identified. The IL-17 signaling pathway activated by infectious gastrointestinal microbes and predicted by the network analysis to be a major pathway modulated by CSCC against UC was studied in a dextran sulfate sodium-induced colitis model. CSCC showed remarkable efficacy against UC with respect to the attenuation of colon length, body weight loss, and disease activity index through gut microbiota recovery and intestinal immune homeostasis. The rectal administration of CSCC reduced the numbers of Th17 cells isolated from both mesenteric lymph nodes and lamina propria mononuclear cells and the levels of IL-17A, IL-6, IL-1β, and TNF-α. Additionally, the percentage of Treg cells and the levels of their hallmark cytokines were upregulated. Rectal administration of CSCC led to microbiota regulation with a significant correlation between suppression of Verrucomicrobiaceae and Ruminococcaceae, as well as the elevation of Lactobacillaceae, and CSCC administration via microbiome correlation heatmaps and cooccurrence network analysis at multiple time points. Thus, our study presents an effective herbal formula, CSCC, for UC treatment and explores its components and mechanisms of efficacy through the examination of gut microbiota and hallmark cytokines in the IL-17 pathway.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>32382313</pmid><doi>10.1155/2020/9521073</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-1697-5633</orcidid><orcidid>https://orcid.org/0000-0001-8418-2507</orcidid><orcidid>https://orcid.org/0000-0001-6460-8291</orcidid><orcidid>https://orcid.org/0000-0002-2870-9893</orcidid><orcidid>https://orcid.org/0000-0002-5986-7739</orcidid><orcidid>https://orcid.org/0000-0002-3297-1798</orcidid><orcidid>https://orcid.org/0000-0001-9660-0008</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1741-427X |
| ispartof | Evidence-based complementary and alternative medicine, 2020, Vol.2020 (2020), p.1-16 |
| issn | 1741-427X 1741-4288 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7189316 |
| source | Wiley Online Library Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Alternative medicine Body weight Body weight loss Clinical trials Colon Cytokines Dextran Dextran sulfate Digestive system Flow cytometry Gastrointestinal tract Genes Health aspects Helper cells Herbal medicine Homeostasis IL-1β Immune system Immunoglobulins Inflammatory bowel disease Interleukin 17 Interleukin 6 Intestinal microflora Intestine Laboratory animals Lamina propria Leukocytes (mononuclear) Lymph nodes Lymphocytes T Medicine, Botanic Medicine, Herbal Microbiota Microbiota (Symbiotic organisms) Ontology Pharmacology Polysaccharides Proteins Rectum Signal transduction Software Tumor necrosis factor-α Ulcerative colitis |
| title | Integrating Pharmacology and Microbial Network Analysis with Experimental Validation to Reveal the Mechanism of Composite Sophora Colon-Soluble Capsule against Ulcerative Colitis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T18%3A24%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Integrating%20Pharmacology%20and%20Microbial%20Network%20Analysis%20with%20Experimental%20Validation%20to%20Reveal%20the%20Mechanism%20of%20Composite%20Sophora%20Colon-Soluble%20Capsule%20against%20Ulcerative%20Colitis&rft.jtitle=Evidence-based%20complementary%20and%20alternative%20medicine&rft.au=Tong,%20Zhanqi&rft.date=2020&rft.volume=2020&rft.issue=2020&rft.spage=1&rft.epage=16&rft.pages=1-16&rft.issn=1741-427X&rft.eissn=1741-4288&rft_id=info:doi/10.1155/2020/9521073&rft_dat=%3Cgale_pubme%3EA628849580%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2397490065&rft_id=info:pmid/32382313&rft_galeid=A628849580&rfr_iscdi=true |